##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479225
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5213429
Total Bases	844.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.29855935508089	40.0	39.0	41.0	36.0	41.0
2	39.494677303555875	40.0	39.0	41.0	37.0	41.0
3	39.504901860176865	40.0	39.0	41.0	37.0	41.0
4	39.554266683213676	40.0	39.0	41.0	37.0	41.0
5	39.526909065031866	40.0	39.0	41.0	37.0	41.0
6	39.54346457964614	40.0	39.0	41.0	37.0	41.0
7	39.496198950824876	40.0	39.0	41.0	37.0	41.0
8	39.445218684286296	40.0	39.0	41.0	37.0	41.0
9	39.433108996017786	40.0	39.0	41.0	37.0	41.0
10-14	39.25197627127942	40.0	38.4	41.0	36.0	41.0
15-19	39.047465497276356	40.0	38.0	41.0	36.0	41.0
20-24	38.98889502475242	40.0	38.0	41.0	35.4	41.0
25-29	38.693107434665365	40.0	38.0	41.0	35.0	41.0
30-34	38.265236565032346	39.6	36.8	40.6	34.0	41.0
35-39	37.866756984702384	39.0	36.0	40.0	33.4	41.0
40-44	37.415816576767426	38.2	35.0	40.0	33.0	41.0
45-49	36.79081487443293	37.2	35.0	40.0	33.0	41.0
50-54	36.365956992988686	36.4	34.6	39.4	32.6	41.0
55-59	35.916225961838165	35.6	34.0	39.0	32.0	40.0
60-64	35.41252327402944	35.0	33.8	38.0	31.4	40.0
65-69	35.54193852069339	35.0	34.0	37.2	32.2	40.0
70-74	35.280719349971	35.0	34.0	37.0	32.0	40.0
75-79	35.121075514790746	35.0	34.0	36.0	32.0	40.0
80-84	35.06270625340827	35.0	34.0	36.0	31.2	40.0
85-89	34.95716155336536	34.8	33.6	36.8	30.6	40.2
90-94	38.96958682913092	40.0	38.0	41.0	35.0	41.0
95-99	38.892590130977126	40.0	38.0	41.0	35.0	41.0
100-104	38.7401779262717	40.0	38.0	41.0	34.2	41.0
105-109	38.53473146954606	40.0	37.6	41.0	34.0	41.0
110-114	38.26554795474946	40.0	36.6	41.0	34.0	41.0
115-119	37.97336952633733	39.2	35.8	41.0	33.2	41.0
120-124	37.5946085295905	38.8	35.0	40.0	33.0	41.0
125-129	36.970786929376864	37.8	35.0	39.6	32.8	40.6
130-134	36.997761170906415	37.8	35.0	40.0	33.0	41.0
135-139	36.664664621746894	37.0	35.0	40.0	33.0	41.0
140-144	36.30335458616503	36.0	34.8	39.2	32.8	41.0
145-149	35.80395017601628	35.0	34.0	38.4	32.0	40.0
150-154	35.27580192379649	35.0	34.0	37.0	32.0	39.4
155-159	34.78805944939923	35.0	34.0	36.0	31.6	38.2
160-164	34.405571124074164	35.0	34.0	35.4	31.0	37.0
165-169	34.060330144298874	35.0	34.0	35.0	31.0	36.0
170-174	33.79935088142973	35.0	33.0	35.0	31.0	35.8
175-178	33.07694057719912	33.5	32.0	34.75	29.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	89.0
31	1638.0
32	13638.0
33	73518.0
34	281768.0
35	784260.0
36	1406361.0
37	1484530.0
38	966092.0
39	200828.0
40	705.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.363219677490573	27.610676197949562	30.601452518102768	21.424651606457093
2	20.983713406282124	28.656226065416828	29.28575415527861	21.074306373022438
3	20.5937781065015	28.96882263094021	29.672601276434378	20.764797986123913
4	20.58528081997472	28.75809759756966	29.546312033788126	21.110309548667487
5	20.755073100640672	29.177917259446712	29.23856832038952	20.8284413195231
6	20.541336613580043	29.175423699066393	29.563824500151437	20.719415187202127
7	20.488626583386864	28.74275261061386	29.516945565001464	21.251675240997816
8	20.858575037657555	28.990420700080506	29.2273089362107	20.92369532605124
9	20.711570062620975	29.038738227757587	29.38931363599658	20.860378073624865
10-14	20.794436828429046	28.85993843974858	29.161463597183353	21.184161134639027
15-19	20.796711722745243	28.917087007418722	29.115056520382264	21.171144749453767
20-24	20.877595148989272	28.963210201961125	28.988663699074063	21.170530949975536
25-29	20.838001246396566	28.883040317610543	28.994544665324874	21.28441377066802
30-34	20.792177279099803	28.873672203074026	28.986304407329612	21.347846110496565
35-39	20.94238552016341	28.906656252535516	28.884452056410474	21.266506170890597
40-44	21.05982837782964	28.739744993170525	28.772180459348352	21.42824616965149
45-49	21.29019115825688	28.66779618558151	28.686850823133874	21.355161833027744
50-54	21.266847596850365	28.707723841640505	28.701865892870128	21.32356266863901
55-59	21.186689221239995	28.668586452409727	28.73628086236525	21.408443463985027
60-64	21.064124974177265	28.757752335363158	28.837972090921348	21.340150599538234
65-69	21.059598203025303	28.799835962089443	28.810941896398706	21.32962393848655
70-74	20.99626176936523	28.689624429526134	28.833414629795477	21.480699171313162
75-79	20.938591472138587	28.73799182841082	28.836126856239915	21.48728984321068
80-84	20.974099771954315	28.758101433816403	28.81978828137872	21.448010512850562
85-89	20.946954490029498	28.615166716569846	28.880055717647636	21.55782307575302
90-94	20.66150926062909	28.80096451467059	29.363288421378797	21.17423780332152
95-99	20.78459177190494	28.950236055696248	29.111360833759676	21.153811338639137
100-104	20.923295138412033	28.967616580612226	28.920991857714295	21.18809642326144
105-109	20.927942506731082	28.952493882828445	28.87390717810773	21.245656432332744
110-114	20.909119080568285	28.982698123317768	28.894111075437646	21.2140717206763
115-119	20.888015394283993	29.06356798126551	28.878639466325485	21.169777158125015
120-124	20.853900589599448	29.077297418194924	28.88511844655815	21.18368354564748
125-129	20.81746201939993	29.114573682468237	28.9581458679897	21.109818430142138
130-134	20.88829281065788	29.188929124169483	28.917091904618967	21.005686160553672
135-139	20.86244625285771	29.23342140540781	28.987196359934806	20.916935981799675
140-144	20.726296421237443	29.369002674532123	29.131103291810785	20.77359761241965
145-149	20.602250000037568	29.60711377521327	29.25388237230493	20.53675385244423
150-154	20.43766253818111	29.751848279713656	29.37921246478145	20.431276717323783
155-159	20.23655921288176	29.91679809474605	29.593688959588132	20.252953732784054
160-164	19.991979797629384	30.13540095735404	29.80325152977597	20.069367715240606
165-169	19.73523992506302	30.374782787671702	30.02188234983419	19.868094937431092
170-174	19.529385439365665	30.677807690457104	30.275725628847418	19.517081241329816
175-178	19.278307483421088	30.948169553592376	30.617025733842656	19.15649722914388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	2.0
3	4.0
4	7.0
5	8.5
6	8.5
7	15.5
8	41.5
9	98.0
10	217.5
11	462.5
12	910.5
13	1741.0
14	3135.5
15	5183.0
16	8142.5
17	12301.5
18	17711.0
19	24336.5
20	32165.0
21	40985.5
22	50717.5
23	61116.5
24	71211.5
25	81022.5
26	90203.5
27	98283.0
28	105225.5
29	111516.0
30	116622.5
31	120980.0
32	125134.5
33	128090.0
34	131066.5
35	134479.0
36	138306.5
37	142975.5
38	147617.5
39	151723.5
40	155204.0
41	158121.5
42	160288.0
43	160330.5
44	158960.5
45	156555.5
46	152291.0
47	146751.0
48	140641.0
49	134179.0
50	126983.0
51	120012.5
52	113627.0
53	107441.5
54	101727.5
55	96911.0
56	92253.5
57	87240.0
58	82781.0
59	78417.5
60	73819.0
61	68716.5
62	63390.0
63	57807.5
64	51363.5
65	44854.0
66	38457.0
67	32067.0
68	26073.0
69	20666.0
70	15978.0
71	11899.5
72	8643.0
73	6067.5
74	4023.5
75	2636.5
76	1671.0
77	1035.5
78	638.5
79	361.0
80	202.0
81	99.5
82	48.0
83	26.5
84	13.0
85	5.0
86	2.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2573.0
92-93	5317.0
94-95	7831.0
96-97	10225.0
98-99	12913.0
100-101	15747.0
102-103	18164.0
104-105	20826.0
106-107	22714.0
108-109	24128.0
110-111	25527.0
112-113	27269.0
114-115	28815.0
116-117	30399.0
118-119	31797.0
120-121	33882.0
122-123	35499.0
124-125	36915.0
126-127	38933.0
128-129	41572.0
130-131	43926.0
132-133	48754.0
134-135	106254.0
136-137	98984.0
138-139	96026.0
140-141	99551.0
142-143	102184.0
144-145	103750.0
146-147	100098.0
148-149	98666.0
150-151	89034.0
152-153	88332.0
154-155	90317.0
156-157	91954.0
158-159	91988.0
160-161	93586.0
162-163	99176.0
164-165	101629.0
166-167	106170.0
168-169	111963.0
170-171	118935.0
172-173	124312.0
174-175	133644.0
176-177	434750.0
178-179	2068400.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.98941236773001
#Duplication Level	Percentage of total
1	92.19659626955476
2	7.198035078087741
3	0.531842086657438
4	0.057612617098234335
5	0.008310420074567601
6	0.0
7	0.0010590048079805797
8	0.0010692012723849092
9	0.0
>10	0.0038959043227714332
>50	0.0015794181241296065
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.9181233694752533E-5	1.9181233694752533E-5	0.0	0.0	0.0
2	0.0	1.9181233694752533E-5	1.9181233694752533E-5	0.0	0.0	0.0
3	0.0	1.9181233694752533E-5	1.9181233694752533E-5	0.0	0.0	0.0
4	1.9181233694752533E-5	1.9181233694752533E-5	1.9181233694752533E-5	0.0	0.0	0.0
5	1.9181233694752533E-5	1.9181233694752533E-5	1.9181233694752533E-5	0.0	0.0	0.0
6	1.9181233694752533E-5	3.8362467389505065E-5	1.9181233694752533E-5	0.0	0.0	0.0
7	3.8362467389505065E-5	3.8362467389505065E-5	3.8362467389505065E-5	0.0	0.0	0.0
8	3.8362467389505065E-5	3.8362467389505065E-5	3.8362467389505065E-5	0.0	0.0	0.0
9	3.8362467389505065E-5	3.8362467389505065E-5	3.8362467389505065E-5	1.9181233694752533E-5	0.0	0.0
10-14	4.987120760635658E-5	3.8362467389505065E-5	5.75437010842576E-5	1.9181233694752533E-5	7.672493477901012E-6	0.0
15-19	9.974241521271319E-5	5.3707454345307084E-5	8.439742825691113E-5	3.068997391160405E-5	1.9181233694752533E-5	0.0
20-24	1.150874021685152E-4	1.0357866195166369E-4	1.074149086906142E-4	3.8362467389505065E-5	3.8362467389505065E-5	0.0
25-29	1.3810488260221823E-4	1.3810488260221823E-4	1.841398434696243E-4	5.37074543453071E-5	4.603496086740608E-5	0.0
30-34	9.974241521271319E-4	1.8030359673067378E-4	2.1099357064227787E-4	7.672493477901013E-5	8.439742825691113E-5	7.672493477901012E-6
35-39	0.003352879649842743	1.9181233694752533E-4	2.1099357064227787E-4	1.074149086906142E-4	1.3043238912431723E-4	4.2198714128455573E-5
40-44	0.005562557771478234	2.0332107716437684E-4	2.1099357064227787E-4	1.6112236303592126E-4	2.2633855759807988E-4	7.672493477901013E-5
45-49	0.0077377096724631726	2.3401105107598088E-4	2.1099357064227787E-4	1.7263110325277278E-4	2.7620976520443646E-4	7.672493477901013E-5
50-54	0.00954074563976991	2.531922847707334E-4	2.2633855759807993E-4	1.8797609020857479E-4	3.299172195497436E-4	9.206992173481215E-5
55-59	0.011006191894049003	2.6853727172653543E-4	2.7237351846548595E-4	2.1099357064227787E-4	4.1431464780665466E-4	9.590616847376266E-5
60-64	0.01250616436897865	2.7620976520443646E-4	3.1457223259394155E-4	2.1866606412017888E-4	4.450046217182588E-4	1.0357866195166369E-4
65-69	0.013775962039571269	3.222447260718426E-4	3.2608097281079304E-4	2.301748043370304E-4	4.795308423688133E-4	1.150874021685152E-4
70-74	0.02415300946843239	3.529346999834466E-4	3.2608097281079304E-4	2.301748043370304E-4	4.987120760635658E-4	1.3426863586326772E-4
75-79	0.2044412612121504	4.028059075898031E-4	3.2608097281079304E-4	2.301748043370304E-4	5.485832836699225E-4	1.5344986955802026E-4
80-84	0.2747251377164627	4.411683749793083E-4	3.3758971302764453E-4	2.9539099889918903E-4	8.209568021354084E-4	1.5344986955802026E-4
85-89	0.2752583760131767	4.411683749793083E-4	3.5677094672239716E-4	3.4526220650554556E-4	0.0013503588521105781	1.5344986955802026E-4
90-94	0.27566118192076655	4.5267711519615986E-4	3.6444344020029814E-4	3.6444344020029814E-4	0.001392557566239034	1.5344986955802026E-4
95-99	0.27609467780226793	4.795308423688133E-4	4.028059075898031E-4	3.6444344020029814E-4	0.0014002300597169349	1.5344986955802026E-4
100-104	0.27667011481311055	4.795308423688133E-4	4.488408684572093E-4	3.7978842715610014E-4	0.0014155750466727367	1.5344986955802026E-4
105-109	0.27714197316200145	5.178933097583183E-4	4.872033358467143E-4	3.8362467389505065E-4	0.00143859252710644	1.5344986955802026E-4
110-114	0.277874696289141	5.370745434530709E-4	5.178933097583183E-4	4.066421543287537E-4	0.0014501012673232916	1.5344986955802026E-4
115-119	0.27859974692280265	5.370745434530709E-4	5.178933097583183E-4	4.411683749793083E-4	0.0014731187477569946	1.5728611629697078E-4
120-124	0.28109330730312043	5.370745434530709E-4	5.639282706257244E-4	4.948758293246153E-4	0.00151531746188545	1.841398434696243E-4
125-129	0.2858042182985517	5.562557771478235E-4	5.869457510594276E-4	5.178933097583183E-4	0.0015498436825360045	1.9181233694752533E-4
130-134	0.2915278984330658	6.176357249710316E-4	6.176357249710315E-4	5.178933097583183E-4	0.0015728611629697077	2.1099357064227787E-4
135-139	0.2967375215045606	6.790156727942396E-4	6.713431793163386E-4	5.178933097583183E-4	0.0016150598770981635	2.1099357064227787E-4
140-144	0.30065816567176806	7.097056467058436E-4	6.905244130110911E-4	5.178933097583183E-4	0.0016419136042708168	2.1099357064227787E-4
145-149	0.304617172306365	7.135418934447942E-4	7.058693999668931E-4	5.178933097583183E-4	0.001653422344487668	2.1866606412017888E-4
150-154	0.30897514860181274	7.288868804005963E-4	7.097056467058436E-4	5.178933097583183E-4	0.0016687673314434704	2.301748043370304E-4
155-159	0.31642130352211567	7.595768543122003E-4	7.288868804005963E-4	5.409107901920213E-4	0.0016764398249213713	2.301748043370304E-4
160-164	0.32553238952712316	7.672493477901013E-4	7.442318673563983E-4	5.792732575815266E-4	0.001687948565138223	2.301748043370304E-4
165-167	0.3299555820171331	8.120055597445238E-4	7.480681140953488E-4	5.946182445373285E-4	0.0016879485651382228	2.3017480433703037E-4
>>END_MODULE
