##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479226
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8652670
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-180
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.266371536184785	40.0	39.0	41.0	36.0	41.0
2	39.45891441601263	40.0	39.0	41.0	37.0	41.0
3	39.457173797220975	40.0	39.0	41.0	37.0	41.0
4	39.522434231283526	40.0	39.0	41.0	37.0	41.0
5	39.50147052875008	40.0	39.0	41.0	37.0	41.0
6	39.50083615808762	40.0	39.0	41.0	37.0	41.0
7	39.46208407347096	40.0	39.0	41.0	37.0	41.0
8	39.41129570410058	40.0	39.0	41.0	37.0	41.0
9	39.3949302354071	40.0	39.0	41.0	37.0	41.0
10-14	39.21675915064368	40.0	38.2	41.0	36.0	41.0
15-19	38.99840296694546	40.0	38.0	41.0	35.8	41.0
20-24	38.949137595678565	40.0	38.0	41.0	35.2	41.0
25-29	38.642500892788	40.0	38.0	41.0	34.8	41.0
30-34	38.21357474629218	39.6	36.8	40.4	34.0	41.0
35-39	37.81892245977254	39.0	35.8	40.0	33.0	41.0
40-44	37.38018324979458	38.2	35.0	40.0	33.0	41.0
45-49	36.74299019840119	37.2	35.0	40.0	32.6	41.0
50-54	36.340942021364505	36.4	34.4	39.4	32.0	41.0
55-59	35.89784644508573	35.6	34.0	39.0	32.0	40.0
60-64	35.41004792740275	35.0	33.8	38.2	31.4	40.0
65-69	35.53312528965048	35.0	34.0	37.4	32.0	40.0
70-74	35.29813874792406	35.0	34.0	37.0	32.0	40.0
75-79	35.181364503673436	35.0	34.0	37.0	31.6	40.0
80-84	35.171216953842	35.0	34.0	37.0	31.0	40.0
85-89	35.1219752284555	34.8	33.6	38.0	30.6	40.2
90-94	38.97611713091292	40.0	38.0	41.0	35.0	41.0
95-99	38.85526545567762	40.0	38.0	41.0	35.0	41.0
100-104	38.75553048205994	40.0	38.0	41.0	34.2	41.0
105-109	38.55246258221679	40.0	37.6	41.0	34.0	41.0
110-114	38.31014442365845	40.0	36.8	41.0	34.0	41.0
115-119	38.02469917340943	39.4	36.0	41.0	33.2	41.0
120-124	37.70911264344415	39.0	35.2	40.4	33.0	41.0
125-129	37.05808213020289	38.0	35.0	39.8	32.8	40.6
130-134	37.07419211096778	38.0	35.0	40.0	33.0	41.0
135-139	36.72588634456919	37.0	35.0	40.0	32.4	41.0
140-144	36.45194048313732	36.4	35.0	39.6	32.6	41.0
145-149	35.992070796672785	35.4	34.0	38.8	32.0	40.4
150-154	35.46897149780568	35.0	34.0	37.4	32.0	39.8
155-159	34.95013354615769	35.0	34.0	36.4	31.6	38.8
160-164	34.52834285727732	35.0	34.0	35.8	31.0	37.2
165-169	34.135424906797574	35.0	34.0	35.0	31.0	36.4
170-174	33.83717479430958	35.0	33.0	35.0	31.0	36.0
175-179	33.61628744464578	34.0	33.0	35.0	30.8	35.2
180	31.424593938793702	32.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	159.0
31	2691.0
32	22743.0
33	120647.0
34	460524.0
35	1274016.0
36	2324122.0
37	2487821.0
38	1621090.0
39	337655.0
40	1201.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.196690732455995	27.800736651230196	30.747503371791595	21.25506924452221
2	20.79136266609035	28.79164465997201	29.473468883015304	20.943523790922338
3	20.45753507298903	29.125934538125225	29.819292773213355	20.597237615672388
4	20.4027774085918	28.961291716892013	29.70500435125805	20.93092652325814
5	20.548616785339092	29.36879599013946	29.44895621813845	20.633631006383002
6	20.418240843577763	29.322844855980872	29.721195885200753	20.53771841524061
7	20.331331253821077	28.970629874940336	29.660289829613284	21.0377490416253
8	20.643188749830976	29.153278698944952	29.427101692310004	20.77643085891407
9	20.54152070979247	29.21083318790616	29.571219057238977	20.67642704506239
10-14	20.612224897054897	29.022838037276355	29.335828131663405	21.029108934005343
15-19	20.627401715308686	29.05539446205622	29.302823290383202	21.014380532251895
20-24	20.69128488663037	29.103698627128967	29.18306141341343	21.021955072827232
25-29	20.656481756498284	29.053203230910228	29.184966027827247	21.10534898476424
30-34	20.619561360828506	29.022935117137255	29.162942767954863	21.19456075407938
35-39	20.737628963083072	29.070562034608972	29.076698868672906	21.11511013363505
40-44	20.85461019546568	28.91395141615247	28.9690973999933	21.262340988388555
45-49	21.121489667351234	28.8330885148746	28.85896954350507	21.1864522742691
50-54	21.08780757847	28.88006361042314	28.898007204712535	21.134121606394327
55-59	20.983989912940167	28.851688553937688	28.938505686683996	21.225815846438152
60-64	20.851011306336655	28.905933081927316	29.063558416072727	21.179497195663306
65-69	20.861627682553475	28.970322455380824	29.0386620546028	21.129387807462898
70-74	20.789469608802833	28.869787013719467	29.062467423350245	21.278275954127455
75-79	20.73894647548098	28.88415945598295	29.098826142681972	21.2780679258541
80-84	20.75491842402403	28.916907729059353	29.090558174528784	21.23761567238783
85-89	20.73052364183541	28.837339225926794	29.1494648472668	21.282672284970996
90-94	20.454775382525618	28.980710529058456	29.582042111274877	20.982471977141053
95-99	20.59240501826906	29.175636979359204	29.282947577094465	20.94901042527727
100-104	20.727401238097432	29.061807957066755	29.14742707183399	21.06336373300182
105-109	20.773531490785533	29.160961461132363	29.048742975063945	21.01676407301816
110-114	20.760772099859953	29.12763156165924	29.04638769452775	21.065208643953053
115-119	20.717699772649077	29.19858860360302	29.086866988409753	20.99684463533815
120-124	20.696312863227366	29.272488062581036	29.085498972162462	20.94570010202914
125-129	20.64289572139053	29.301717429397367	29.121072878361304	20.9343139708508
130-134	20.67496131761437	29.336260930299886	29.151972175763834	20.836805576321915
135-139	20.676889390043858	29.457106861940147	29.191825385647736	20.67417836236826
140-144	20.54104967783615	29.551160803537137	29.333501212121423	20.5742883065053
145-149	20.39148236826358	29.728981513499914	29.48788340561207	20.39165271262444
150-154	20.23916704353028	29.974586707680512	29.616742693110243	20.16950355567897
155-159	20.04123419648368	30.117717616904837	29.78906001307789	20.051988173533598
160-164	19.80744489659387	30.278427021049893	30.037012746029077	19.877115336327158
165-169	19.569625625804058	30.58738721344104	30.2221299440248	19.620857216730098
170-174	19.351727537640105	30.788500178962025	30.46299046654478	19.396781816853085
175-179	19.102276432604572	31.140210971429173	30.719210293180943	19.03830230278531
180	18.803038161413237	31.148680170764635	31.603902670412786	18.444378997409338
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	5.5
2	4.5
3	7.5
4	12.0
5	15.0
6	21.5
7	39.5
8	82.5
9	195.0
10	421.5
11	829.5
12	1675.5
13	3166.5
14	5565.5
15	9322.0
16	14669.5
17	22016.5
18	31660.5
19	43532.5
20	57428.0
21	73294.5
22	90319.5
23	107835.5
24	125101.5
25	141538.5
26	156885.5
27	170584.0
28	182560.0
29	192354.5
30	199951.5
31	206198.5
32	211810.0
33	216855.0
34	221832.5
35	227290.0
36	233192.0
37	239542.0
38	245732.0
39	251502.5
40	257121.0
41	262029.0
42	264431.0
43	264089.0
44	261431.5
45	256538.5
46	249051.5
47	239774.0
48	229616.5
49	218115.5
50	206073.5
51	194570.5
52	183744.0
53	173439.0
54	164367.0
55	156061.0
56	148227.0
57	140662.0
58	133379.5
59	126258.0
60	118531.5
61	110199.0
62	101469.5
63	92039.0
64	81673.5
65	71229.5
66	60957.5
67	50727.0
68	41009.0
69	32185.5
70	24617.5
71	18326.0
72	13272.5
73	9252.5
74	6178.5
75	4012.0
76	2472.5
77	1471.5
78	888.0
79	513.0
80	278.0
81	153.5
82	79.5
83	39.0
84	17.0
85	10.0
86	4.0
87	1.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	4350.0
92-93	9786.0
94-95	14262.0
96-97	18168.0
98-99	23078.0
100-101	28023.0
102-103	32375.0
104-105	36248.0
106-107	40166.0
108-109	42898.0
110-111	45831.0
112-113	48581.0
114-115	51164.0
116-117	54781.0
118-119	57639.0
120-121	60939.0
122-123	64395.0
124-125	67485.0
126-127	71415.0
128-129	75682.0
130-131	79289.0
132-133	84821.0
134-135	136199.0
136-137	176750.0
138-139	166428.0
140-141	159893.0
142-143	167124.0
144-145	176157.0
146-147	169953.0
148-149	167358.0
150-151	165096.0
152-153	152204.0
154-155	150529.0
156-157	153553.0
158-159	155230.0
160-161	158093.0
162-163	162664.0
164-165	169878.0
166-167	180079.0
168-169	184978.0
170-171	193329.0
172-173	202369.0
174-175	210877.0
176-177	232768.0
178-179	780297.0
180-181	3069488.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.13552493908897
#Duplication Level	Percentage of total
1	88.71585105500893
2	10.043156816913882
3	1.088867229124325
4	0.12406676391301782
5	0.015343282352970654
6	0.00411541274863378
7	0.0
8	0.0010408572502533797
9	0.0
>10	0.004650380333497193
>50	0.002908202354473366
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.1557126297431892E-5	0.0	1.1557126297431892E-5	0.0	0.0	0.0
2	1.1557126297431892E-5	0.0	1.1557126297431892E-5	0.0	0.0	0.0
3	1.1557126297431892E-5	0.0	1.1557126297431892E-5	0.0	0.0	0.0
4	1.1557126297431892E-5	1.1557126297431892E-5	1.1557126297431892E-5	0.0	1.1557126297431892E-5	0.0
5	1.1557126297431892E-5	3.467137889229567E-5	1.1557126297431892E-5	1.1557126297431892E-5	1.1557126297431892E-5	0.0
6	1.1557126297431892E-5	3.467137889229567E-5	1.1557126297431892E-5	3.467137889229567E-5	1.1557126297431892E-5	0.0
7	1.1557126297431892E-5	4.622850518972757E-5	2.3114252594863784E-5	5.7785631487159456E-5	1.1557126297431892E-5	0.0
8	2.3114252594863784E-5	6.934275778459134E-5	2.3114252594863784E-5	5.7785631487159456E-5	1.1557126297431892E-5	0.0
9	4.622850518972757E-5	6.934275778459134E-5	2.3114252594863784E-5	5.7785631487159456E-5	1.1557126297431892E-5	0.0
10-14	4.622850518972757E-5	7.627703356305048E-5	3.9294229411268425E-5	7.165418304407773E-5	1.1557126297431892E-5	0.0
15-19	7.627703356305048E-5	1.2250553875277803E-4	5.31627809681867E-5	8.783415986048237E-5	1.3868551556918271E-5	0.0
20-24	1.2250553875277803E-4	1.5255406712610096E-4	7.396560830356411E-5	9.245701037945513E-5	4.160565467075481E-5	0.0
25-29	1.5948834290456008E-4	1.6642261868301923E-4	1.0170271141740067E-4	1.063255619363734E-4	5.31627809681867E-5	0.0
30-34	7.350332325166683E-4	2.010939975753149E-4	1.3406266505020992E-4	1.4330836608815545E-4	6.934275778459134E-5	0.0
35-39	0.00269974470308009	2.1727397439171955E-4	1.4561979134764184E-4	1.7797974498045112E-4	1.2250553875277803E-4	9.245701037945514E-6
40-44	0.0051983954085848645	2.727481806193926E-4	1.5024264186661458E-4	2.126511238727468E-4	2.0571684809428766E-4	2.7737103113836536E-5
45-49	0.007447412186065111	3.0048528373322915E-4	1.5486549238558734E-4	2.4269965224606968E-4	2.843053069168245E-4	6.240848200613221E-5
50-54	0.009127818349711709	3.1204241003066107E-4	1.6179976816404646E-4	2.658139048409335E-4	3.6289376573936133E-4	8.783415986048237E-5
55-59	0.01068340754934604	3.6751661625833405E-4	1.9878257231582854E-4	2.658139048409335E-4	4.322365235239527E-4	9.245701037945513E-5
60-64	0.012042525601924032	4.0681084566960256E-4	2.2883110068915147E-4	2.727481806193926E-4	5.431849359792988E-4	9.245701037945513E-5
65-69	0.012994832808832419	4.1836797196703453E-4	2.3807680172709694E-4	2.773710311383654E-4	6.287076705802949E-4	1.2019411349329166E-4
70-74	0.02268201607133983	4.345479487834391E-4	2.681253301004199E-4	2.981738584737428E-4	7.072961294028316E-4	1.386855155691827E-4
75-79	0.18716766038690946	4.599736266377893E-4	3.051081342522019E-4	3.3515666262552484E-4	7.858845882253686E-4	1.594883429045601E-4
80-84	0.2519083704798635	4.7846502871368027E-4	3.2359953632809293E-4	3.467137889229567E-4	0.0010909927224775704	1.6179976816404646E-4
85-89	0.2524561782663617	5.154478328654623E-4	3.397795131444976E-4	3.6982804151782054E-4	0.0015509663491153599	1.6179976816404646E-4
90-94	0.2529300204445565	5.524306370172443E-4	3.6520519099884766E-4	3.7445089203679325E-4	0.00160181770482406	1.6179976816404646E-4
95-99	0.2534154197490486	6.102162685044038E-4	3.883194435937116E-4	3.929422941126843E-4	0.0016179976816404647	1.6179976816404646E-4
100-104	0.2539331790071735	6.518219231751587E-4	4.206793972265208E-4	4.114336961885753E-4	0.0016295548079378966	1.6179976816404646E-4
105-109	0.25432149845076724	6.72624750510536E-4	4.322365235239527E-4	4.206793972265208E-4	0.001638800508975842	1.6179976816404646E-4
110-114	0.2548022749047404	7.165418304407771E-4	4.3917079930241187E-4	4.2761367300498E-4	0.0016619147615707058	1.6179976816404646E-4
115-119	0.2554217368742827	7.489017840735867E-4	4.3917079930241187E-4	4.322365235239527E-4	0.0017058318415009471	1.6179976816404646E-4
120-124	0.2569033604656135	7.951302892633142E-4	4.4610507508087096E-4	4.437936498213846E-4	0.0017728631740260522	1.6179976816404646E-4
125-129	0.26095066609497414	8.482930702315008E-4	4.6228505189727564E-4	4.6459647715676203E-4	0.0018491402075891026	1.6179976816404646E-4
130-134	0.26602655596480623	8.66784472307392E-4	4.900221550111121E-4	4.8539930449213937E-4	0.0018768773107029392	1.6179976816404646E-4
135-139	0.27169185927580736	8.66784472307392E-4	5.085135570870032E-4	4.8539930449213937E-4	0.0019069258390762623	1.6179976816404646E-4
140-144	0.27622918706017907	8.829644491237965E-4	5.13136407605976E-4	4.8539930449213937E-4	0.0019231058158926666	1.6179976816404646E-4
145-149	0.2803712611251787	9.199472532755784E-4	5.200706833844351E-4	5.038907065680304E-4	0.0019485314937470167	1.6411119342353285E-4
150-154	0.2851397314354991	9.361272300919831E-4	5.31627809681867E-4	5.085135570870032E-4	0.001971645746341881	1.8491402075891027E-4
155-159	0.29164870496621276	9.453729311299286E-4	5.31627809681867E-4	5.085135570870032E-4	0.0019878257231582853	1.8491402075891027E-4
160-164	0.3001732413231985	9.59241482686847E-4	5.408735107198125E-4	5.108249823464895E-4	0.0020016942747152038	1.8491402075891027E-4
165-169	0.3065134808099696	9.707986089842787E-4	5.547420622767308E-4	5.31627809681867E-4	0.0020664141819808223	1.9184829653736942E-4
>>END_MODULE
