##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479241
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6854723
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.91100428128168	39.0	37.0	39.0	35.0	39.0
2	39.538233857152214	40.0	39.0	41.0	37.0	41.0
3	39.566002448238976	40.0	39.0	41.0	37.0	41.0
4	39.56145944920021	40.0	39.0	41.0	37.0	41.0
5	39.54322165899337	40.0	39.0	41.0	37.0	41.0
6	39.5132039617064	40.0	39.0	41.0	37.0	41.0
7	39.55122665058821	40.0	39.0	41.0	37.0	41.0
8	39.51634792536475	40.0	39.0	41.0	37.0	41.0
9	39.470702171335006	40.0	39.0	41.0	37.0	41.0
10-14	39.316059394376694	40.0	39.0	41.0	36.6	41.0
15-19	39.205638652356924	40.0	38.6	41.0	36.0	41.0
20-24	39.30835288311431	40.0	39.0	41.0	36.6	41.0
25-29	39.1666759400781	40.0	38.6	41.0	36.0	41.0
30-34	38.84677694488895	40.0	38.0	41.0	35.0	41.0
35-39	38.44650662032586	40.0	37.4	40.2	34.4	41.0
40-44	37.85692761034982	39.2	36.0	40.0	33.4	41.0
45-49	37.21284118993576	38.2	35.0	40.0	33.0	41.0
50-54	36.682267073374085	37.2	34.6	40.0	32.0	41.0
55-59	36.21344804159118	36.2	34.0	39.2	32.0	40.6
60-64	35.67023749902076	35.8	33.8	38.8	30.8	40.0
65-69	35.590317012080575	35.0	33.8	38.6	31.6	40.0
70-74	35.520989455007886	35.0	34.0	38.0	31.2	40.2
75-79	35.50125269832201	35.0	34.0	38.4	31.0	41.0
80-84	35.62083807033486	35.0	34.0	39.0	31.0	41.0
85-89	35.73126155498916	35.0	33.8	39.8	30.6	41.0
90-94	39.124219197102164	39.8	38.6	40.8	35.8	41.0
95-99	39.095667643937205	40.0	38.0	41.0	35.0	41.0
100-104	38.87014171804816	40.0	38.0	41.0	35.0	41.0
105-109	38.71117170512686	40.0	38.0	41.0	34.6	41.0
110-114	38.57595850055086	40.0	37.2	41.0	34.0	41.0
115-119	38.328879850354575	40.0	36.4	41.0	34.0	41.0
120-124	38.0344125657737	39.4	35.6	41.0	33.6	41.0
125-129	37.544038961377375	38.6	35.0	40.6	33.0	41.0
130-134	37.419817563687346	38.6	35.0	40.6	33.0	41.0
135-139	37.05388763989836	37.6	35.0	40.0	33.0	41.0
140-144	36.59885673017512	36.6	35.0	39.8	32.6	41.0
145-149	36.09025594271669	35.8	34.4	39.0	32.0	40.8
150-154	35.53294649985329	35.0	34.0	37.4	32.0	39.8
155-159	34.975353631409	35.0	34.0	36.4	31.4	38.8
160-164	34.493518435651524	35.0	34.0	35.8	31.0	37.0
165-169	34.11289390734729	35.0	34.0	35.0	31.0	36.2
170-174	33.804323847512656	35.0	33.0	35.0	31.0	36.0
175-178	33.24894273670829	34.0	32.5	35.0	29.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	43.0
31	847.0
32	8463.0
33	49539.0
34	204385.0
35	639973.0
36	1528137.0
37	2310199.0
38	1737919.0
39	372315.0
40	2901.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.25709864570749	29.419058946656197	28.820974968645707	21.502867438990606
2	20.70222239469049	29.302569921497923	29.728509817245712	20.26669786656587
3	19.790500651886298	28.893348425603776	29.97884816060401	21.337302761905917
4	19.946349400260228	28.35935164703227	30.373831298507614	21.320467654199884
5	20.487056880343673	29.01800700042876	29.535227025220422	20.959709094007152
6	20.767885150136628	28.79681644320274	29.179209721530686	21.256088685129946
7	20.628142085391342	29.122052050826852	29.032522539568706	21.217283324213103
8	20.55986799174817	29.44226922079857	29.16307486093895	20.834787926514316
9	20.743639093804376	29.31003922405034	28.88494546023231	21.06137622191298
10-14	20.5817507140697	29.443144529691427	28.95480969836418	21.020295057874694
15-19	20.608753993414467	29.414481081146533	28.922732545137126	21.054032380301873
20-24	20.564194935375216	29.413319838015333	29.067864594966125	20.95462063164332
25-29	20.525048203990153	29.420193930520604	29.058688440072633	20.996069425416607
30-34	20.52986240290089	29.309884586145934	29.134452260142385	21.02580075081079
35-39	20.49287184908858	29.303599868295187	29.20372712361973	20.999801158996505
40-44	20.59612036839417	29.18824874469763	29.19243563890182	21.023195248006374
45-49	21.008145770441782	28.973477119352598	28.910393607444096	21.107983502761527
50-54	20.8699636732221	29.039866381179806	29.04073585468005	21.049434090918044
55-59	20.769749558078423	29.045894341755314	29.042471883984227	21.14188421618204
60-64	20.668525920011646	29.060182300583115	29.097117418165546	21.17417436123969
65-69	20.584131554258285	29.116490921660876	29.13407295962215	21.165304564458694
70-74	20.599957139041216	29.173488119067688	29.070163739658046	21.156391002233057
75-79	20.617148205697006	29.181908590617013	29.043026246283038	21.157916957402946
80-84	20.65004523158704	29.188099942185847	29.04030695332255	21.121547872904564
85-89	20.68553608949625	29.073265250835078	29.062919099721462	21.178279559947207
90-94	20.4851613680749	29.05464698447298	29.34287255603838	21.117319091413737
95-99	20.666334442586653	29.22111427135889	29.018084428776547	21.09446685727791
100-104	20.68847431879634	29.278934691625924	29.011618292062046	21.020972697515692
105-109	20.631590768282894	29.296522233959767	29.11142363627991	20.960463361477434
110-114	20.626835730739312	29.31455203904438	29.15755347584248	20.90105875437383
115-119	20.55941962655097	29.42801755432208	29.230368725785222	20.78219409334173
120-124	20.497396759598065	29.503632214244213	29.313238758429062	20.685732267728657
125-129	20.446878066284235	29.5838137321377	29.379297447213666	20.590010754364403
130-134	20.420338505790433	29.65824270141034	29.49407444276456	20.427344350034655
135-139	20.327544347920384	29.80168066077305	29.57750563225242	20.293269359054143
140-144	20.201124182095555	29.947911174362083	29.762089794575964	20.0888748489664
145-149	20.044747033716543	30.17046100071674	29.917452225978046	19.867339739588665
150-154	19.780469807436454	30.402733206936674	30.180286997325517	19.636509988301356
155-159	19.67020387139175	30.581857115171886	30.29238387392696	19.455555139509407
160-164	19.50505456079892	30.96981709940475	30.4809722266491	19.04415611314723
165-169	19.279051054657124	31.174044263118006	30.744264102286483	18.802640579938384
170-174	19.15755358989924	31.51025163291379	30.957257438072176	18.37493733911479
175-178	18.897808623475555	31.691665992999006	31.400064911460486	18.010460472064953
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	1.5
3	5.0
4	10.0
5	15.5
6	26.5
7	38.5
8	67.0
9	155.0
10	297.0
11	537.5
12	1025.0
13	1826.5
14	2984.5
15	4723.0
16	7399.5
17	11006.0
18	15557.0
19	21411.0
20	28562.0
21	37053.0
22	46601.0
23	56852.5
24	67621.5
25	78506.0
26	90341.0
27	102484.5
28	114525.0
29	126675.5
30	138828.5
31	150242.0
32	161672.5
33	174008.0
34	185824.0
35	197802.5
36	210097.5
37	221470.5
38	232108.0
39	242748.0
40	251137.5
41	256833.0
42	260823.5
43	261549.5
44	259557.5
45	254772.5
46	246675.0
47	236497.0
48	223708.5
49	209677.5
50	194939.5
51	178784.0
52	163488.5
53	148545.0
54	133848.5
55	120315.5
56	107498.5
57	96197.5
58	85792.5
59	75693.5
60	66705.0
61	58030.5
62	49650.0
63	41961.0
64	34714.0
65	28084.0
66	22223.0
67	17229.0
68	12966.5
69	9492.0
70	6631.5
71	4511.0
72	2967.0
73	1854.0
74	1170.0
75	711.0
76	380.0
77	202.5
78	114.0
79	58.5
80	30.5
81	15.0
82	6.0
83	2.5
84	0.5
85	0.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	3196.0
92-93	7135.0
94-95	11390.0
96-97	15519.0
98-99	19685.0
100-101	24354.0
102-103	28368.0
104-105	32845.0
106-107	37611.0
108-109	41104.0
110-111	44912.0
112-113	48470.0
114-115	52099.0
116-117	55926.0
118-119	59975.0
120-121	63905.0
122-123	67854.0
124-125	71890.0
126-127	76148.0
128-129	79990.0
130-131	84568.0
132-133	89957.0
134-135	148886.0
136-137	148988.0
138-139	151694.0
140-141	153487.0
142-143	153733.0
144-145	154614.0
146-147	156197.0
148-149	158986.0
150-151	160951.0
152-153	148456.0
154-155	150643.0
156-157	150916.0
158-159	158814.0
160-161	163899.0
162-163	166319.0
164-165	171276.0
166-167	175959.0
168-169	185544.0
170-171	187593.0
172-173	200480.0
174-175	210345.0
176-177	330422.0
178-179	2049620.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.25954585419942
#Duplication Level	Percentage of total
1	94.71508257290992
2	4.763057332568275
3	0.42607179555316893
4	0.060295699052586964
5	0.01441302651633125
6	0.01037079328213575
7	0.003134079883748941
8	0.0031570313479329726
9	0.002120061560594014
>10	0.0022976073252926267
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.3765444643058516E-5	1.4588481547686171E-5	2.9176963095372343E-5	1.4588481547686171E-5	1.4588481547686171E-5	0.0
2	7.294240773843086E-5	2.9176963095372343E-5	2.9176963095372343E-5	4.3765444643058516E-5	1.4588481547686171E-5	0.0
3	1.1670785238148937E-4	4.3765444643058516E-5	2.9176963095372343E-5	7.294240773843086E-5	2.9176963095372343E-5	0.0
4	1.3129633392917556E-4	4.3765444643058516E-5	2.9176963095372343E-5	8.753088928611703E-5	2.9176963095372343E-5	0.0
5	1.4588481547686172E-4	4.3765444643058516E-5	2.9176963095372343E-5	8.753088928611703E-5	4.3765444643058516E-5	0.0
6	1.4588481547686172E-4	4.3765444643058516E-5	2.9176963095372343E-5	1.021193708338032E-4	4.3765444643058516E-5	0.0
7	1.604732970245479E-4	4.3765444643058516E-5	2.9176963095372343E-5	1.4588481547686172E-4	4.3765444643058516E-5	0.0
8	1.604732970245479E-4	4.3765444643058516E-5	2.9176963095372343E-5	1.604732970245479E-4	4.3765444643058516E-5	0.0
9	1.7506177857223406E-4	4.3765444643058516E-5	4.3765444643058516E-5	1.604732970245479E-4	4.3765444643058516E-5	0.0
10-14	2.2758031214390432E-4	5.5436229881207456E-5	5.5436229881207456E-5	1.6630868964362237E-4	4.3765444643058516E-5	0.0
15-19	3.617943423826171E-4	6.710701511935639E-5	5.835392619074469E-5	1.8965026011992023E-4	5.5436229881207456E-5	0.0
20-24	4.6974910583549476E-4	7.294240773843086E-5	5.835392619074469E-5	2.450864900011277E-4	7.877780035750532E-5	0.0
25-29	8.723911965516331E-4	9.044858559565427E-5	5.835392619074469E-5	3.0927580881094684E-4	1.2254324500056386E-4	0.0
30-34	0.0030898403917999314	1.021193708338032E-4	6.710701511935639E-5	3.647120386921543E-4	1.6339099333408512E-4	0.0
35-39	0.008875632173612268	1.021193708338032E-4	7.294240773843086E-5	4.493252316687341E-4	1.86732563810383E-4	0.0
40-44	0.015775983945667826	1.283786376196383E-4	7.58601040479681E-5	5.339384246453139E-4	2.800988457155745E-4	5.835392619074468E-6
45-49	0.023181097179273323	1.4588481547686172E-4	1.1087245976241491E-4	5.922923508360587E-4	4.0264209071613836E-4	1.4588481547686173E-5
50-54	0.030379053974901683	1.6339099333408512E-4	1.3129633392917556E-4	6.185516176218937E-4	4.930906763117927E-4	2.3341570476297873E-5
55-59	0.037156862501956676	1.7797947488177131E-4	1.3129633392917556E-4	6.827409364317128E-4	6.09798528693282E-4	2.9176963095372346E-5
60-64	0.04344449804900942	1.8965026011992023E-4	1.3421403023871278E-4	7.206709884556968E-4	7.819426109559787E-4	2.9176963095372346E-5
65-69	0.04953081255070409	1.984033490485319E-4	1.5755560071501067E-4	8.315434482181118E-4	8.928150707183937E-4	4.3765444643058516E-5
70-74	0.07304160941295512	2.1882722321529255E-4	1.604732970245479E-4	8.986504633374682E-4	0.0010270291009571064	5.5436229881207456E-5
75-79	0.3888851526166703	2.1882722321529255E-4	1.7797947488177131E-4	9.657574784568245E-4	0.0012225147536961013	5.835392619074469E-5
80-84	0.497508068524432	2.450864900011277E-4	2.3341570476297872E-4	0.0010036875304808087	0.0014238357990541705	5.835392619074469E-5
85-89	0.49821998642395904	2.625926678583511E-4	2.5675727523927666E-4	0.001021193708338032	0.0017652062672700267	5.835392619074469E-5
90-94	0.4987772664190807	2.771811494060373E-4	2.7134575678696283E-4	0.0010795476345287769	0.0017885478377463245	5.835392619074469E-5
95-99	0.4997605300753947	2.800988457155745E-4	3.1511120143002134E-4	0.0010999715086955375	0.0018089717119130853	5.835392619074469E-5
100-104	0.5006941928944466	2.9176963095372345E-4	3.296996829777075E-4	0.0011379015607195214	0.0018381486750084577	5.835392619074469E-5
105-109	0.5017183042990941	3.005227198823352E-4	3.413704682158564E-4	0.0011729139164339685	0.001878996423341979	5.835392619074469E-5
110-114	0.5030487738162431	3.209465940490958E-4	3.647120386921543E-4	0.0012137616647674896	0.001931514956913649	5.835392619074469E-5
115-119	0.5044200910817256	3.617943423826171E-4	3.647120386921543E-4	0.0012429386278628618	0.001943185742151798	5.835392619074469E-5
120-124	0.5124291674514054	3.647120386921543E-4	3.705474313112288E-4	0.0012983748577440693	0.001957774223699484	5.835392619074469E-5
125-129	0.5295531270920795	3.647120386921543E-4	3.7930052023984047E-4	0.0013859057470301862	0.0019781980978662446	5.835392619074469E-5
130-134	0.5484277045184759	3.647120386921543E-4	3.968066980970639E-4	0.0014588481547686172	0.0020248812388188406	5.835392619074469E-5
135-139	0.5710515217026275	3.676297350016915E-4	4.143128759542873E-4	0.0014851074215544526	0.0020715643797714362	5.835392619074469E-5
140-144	0.5933602276853491	3.7930052023984047E-4	4.3181905381151065E-4	0.0014909428141735269	0.002106576735485883	5.835392619074469E-5
145-149	0.6164742178495032	4.055597870256756E-4	4.376544464305851E-4	0.0015405436514356597	0.002138671394890793	7.294240773843086E-5
150-154	0.640483940780685	4.60996016906883E-4	4.522429279782713E-4	0.0015609675256024204	0.002179519143224314	8.753088928611703E-5
155-159	0.6725581763114279	4.6683140952595754E-4	4.6683140952595754E-4	0.0015901444886977927	0.0022057784100101493	8.753088928611703E-5
160-164	0.7076872398782562	4.6683140952595754E-4	4.6683140952595754E-4	0.0016251568444122396	0.0022320376767959844	8.753088928611703E-5
165-167	0.7221055224745526	4.668314095259575E-4	4.668314095259575E-4	0.0016484984148885373	0.0022612146398913566	8.753088928611703E-5
>>END_MODULE
