##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479242
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6740629
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.914336777769556	39.0	37.0	39.0	35.0	39.0
2	39.569145698420726	40.0	39.0	41.0	37.0	41.0
3	39.59501509428868	40.0	39.0	41.0	37.0	41.0
4	39.590601262879176	40.0	39.0	41.0	37.0	41.0
5	39.57760514634465	40.0	39.0	41.0	37.0	41.0
6	39.5512911332162	40.0	39.0	41.0	37.0	41.0
7	39.56465991526903	40.0	39.0	41.0	37.0	41.0
8	39.53681726141581	40.0	39.0	41.0	37.0	41.0
9	39.48966468856245	40.0	39.0	41.0	37.0	41.0
10-14	39.34246008792355	40.0	39.0	41.0	36.8	41.0
15-19	39.23113934916162	40.0	39.0	41.0	36.0	41.0
20-24	39.32283785385607	40.0	39.0	41.0	36.6	41.0
25-29	39.19439393564013	40.0	38.6	41.0	36.0	41.0
30-34	38.87827652285863	40.0	38.0	41.0	35.0	41.0
35-39	38.468067208564655	40.0	37.4	40.4	34.4	41.0
40-44	37.885224628146716	39.2	36.0	40.0	33.4	41.0
45-49	37.227902915291736	38.2	35.0	40.0	33.0	41.0
50-54	36.69232874261438	37.2	34.6	40.0	32.0	41.0
55-59	36.234460878947644	36.4	34.0	39.2	32.0	40.8
60-64	35.69760994114941	35.8	33.8	38.8	30.8	40.0
65-69	35.63194713727754	35.0	33.8	38.8	31.8	40.0
70-74	35.55201521994461	35.0	34.0	38.0	31.4	40.6
75-79	35.53744865649779	35.0	34.0	38.6	31.0	41.0
80-84	35.66307233345731	35.0	34.0	39.0	31.0	41.0
85-89	35.775437692832526	35.0	33.8	40.0	30.6	41.0
90-94	39.15456220227172	39.8	38.8	40.8	35.8	41.0
95-99	39.12066486671276	40.0	38.0	41.0	35.0	41.0
100-104	38.89622347533051	40.0	38.0	41.0	35.0	41.0
105-109	38.741683216639004	40.0	38.0	41.0	34.6	41.0
110-114	38.598060659325114	40.0	37.2	41.0	34.0	41.0
115-119	38.34484618511733	40.0	36.4	41.0	34.0	41.0
120-124	38.050480862670526	39.6	35.6	41.0	33.8	41.0
125-129	37.55407992806714	38.6	35.0	40.6	33.0	41.0
130-134	37.42897810131633	38.6	35.0	40.8	33.0	41.0
135-139	37.06497208308716	37.6	35.0	40.0	33.0	41.0
140-144	36.60200189115156	36.6	35.0	39.8	32.6	41.0
145-149	36.092084075069224	35.8	34.4	39.0	32.0	40.8
150-154	35.544747140792914	35.0	34.0	37.4	32.0	39.8
155-159	34.99187919788811	35.0	34.0	36.4	31.4	38.8
160-164	34.51391126069122	35.0	34.0	35.8	31.0	37.0
165-169	34.133458623066545	35.0	34.0	35.0	31.0	36.2
170-174	33.85048904958393	35.0	33.4	35.0	31.0	36.0
175-178	33.246046284434556	34.0	32.5	35.0	29.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	47.0
31	786.0
32	7792.0
33	46542.0
34	192118.0
35	608527.0
36	1477292.0
37	2278927.0
38	1742706.0
39	382685.0
40	3207.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.292082534137393	29.388132769211893	28.828897125179264	21.490887571471447
2	20.750570903694594	29.28778605082701	29.714304110195055	20.24733893528334
3	19.827036913023992	28.86824063451645	29.95058769737958	21.35413475507998
4	19.97116292856349	28.338497787075955	30.340744164973326	21.349595119387228
5	20.53889926296196	29.018923901612148	29.505006135184118	20.937170700241772
6	20.787881961757577	28.855675041602204	29.114093061641576	21.242349934998646
7	20.676868583035795	29.07655947241719	28.968231303043083	21.27834064150393
8	20.58831601620561	29.480913428108863	29.099999421418982	20.83077113426655
9	20.769619571111242	29.295574641476335	28.85287114896844	21.08193463844398
10-14	20.613619292798933	29.441368157185334	28.905889939944775	21.039122610070958
15-19	20.62139898220181	29.404893816289253	28.897240895471327	21.07646630603761
20-24	20.610542428607182	29.387669904396162	29.036925189029095	20.96486247796756
25-29	20.548749975707015	29.377860730801235	29.044369598148776	21.02901969534297
30-34	20.53676889797673	29.27650817156678	29.135283962372053	21.051438968084433
35-39	20.509201737701332	29.27948118788321	29.18979816275306	21.021518911662397
40-44	20.61654186871878	29.165293031258656	29.17269886831036	21.045466231712204
45-49	21.03761533233768	28.928297344357627	28.896484289522533	21.13760303378216
50-54	20.887169431814154	28.999317422750902	29.038933903646086	21.07457924178886
55-59	20.79883049489892	29.013446667959325	29.028104053790827	21.15961878335093
60-64	20.709850668238825	29.013538647506042	29.064759386698185	21.21185129755695
65-69	20.617218363449464	29.103408598811775	29.10069965280688	21.17867338493188
70-74	20.64499025239336	29.15528803024169	29.03422514427066	21.16549657309429
75-79	20.63835882378336	29.163492012392318	29.029077256736723	21.169071907087602
80-84	20.664083426042286	29.187107019241083	29.007470964504943	21.141338590211685
85-89	20.71495701662263	29.09912116510195	29.015247686825667	21.17067413144975
90-94	20.509718210101397	29.04622251169232	29.30164213683679	21.142417141369492
95-99	20.698258213560116	29.18327356008933	29.007197553042353	21.1112706733082
100-104	20.722893266862876	29.24204757042465	28.987918193284884	21.047140969427595
105-109	20.66464468994052	29.28433177741723	29.077800212105448	20.973223320536803
110-114	20.655368515674443	29.284627652158417	29.12697766921172	20.93302616295542
115-119	20.582461741914667	29.40883166096016	29.20412820885503	20.80457838827014
120-124	20.507482258268567	29.4713282407566	29.28964496279854	20.731544538176294
125-129	20.475236351989803	29.55410332278455	29.36470642900645	20.605953896219198
130-134	20.458382828648464	29.63622423733448	29.45992394155545	20.445468992461606
135-139	20.36662462490286	29.77425407043514	29.54838924365932	20.31073206100268
140-144	20.23917811378145	29.929403324480518	29.699211239743185	20.132207321994848
145-149	20.05399610426476	30.12684129304518	29.92531731127526	19.8938452914148
150-154	19.823424424700264	30.365213792804024	30.162481349480792	19.64888043301492
155-159	19.689240153753556	30.537701066918988	30.284247506949356	19.488811272378108
160-164	19.513741684738093	30.920849360859613	30.493150244047527	19.07225871035476
165-169	19.28780150833895	31.14481515389989	30.738369935270093	18.829013402491064
170-174	19.18198373460492	31.484451335459145	30.950538279261618	18.38302665067431
175-178	18.949919281937802	31.64976086497085	31.388159752500126	18.01216010059122
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	5.5
4	7.5
5	12.5
6	26.5
7	44.5
8	91.5
9	163.5
10	280.0
11	529.5
12	955.5
13	1779.0
14	2995.5
15	4765.0
16	7311.5
17	10758.5
18	15356.0
19	21303.0
20	28436.5
21	36496.0
22	45428.0
23	55233.0
24	66098.5
25	77028.0
26	88259.5
27	100075.5
28	111730.5
29	123692.0
30	135623.5
31	147740.5
32	159076.5
33	169993.0
34	182338.0
35	194588.5
36	205903.0
37	216961.0
38	227456.0
39	237608.0
40	246251.5
41	251860.0
42	255498.5
43	256459.0
44	254493.5
45	250329.0
46	242797.5
47	232493.5
48	220488.5
49	206701.5
50	191989.5
51	176800.5
52	161700.0
53	146575.5
54	132087.5
55	119048.0
56	106620.5
57	95306.0
58	84776.0
59	75135.0
60	66229.5
61	57643.0
62	49792.0
63	42190.5
64	34719.0
65	28185.0
66	22411.5
67	17251.0
68	12992.5
69	9339.0
70	6546.5
71	4481.5
72	2974.0
73	1900.5
74	1179.0
75	701.5
76	385.5
77	207.5
78	120.5
79	61.5
80	26.0
81	14.5
82	7.0
83	2.0
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	3043.0
92-93	7024.0
94-95	10895.0
96-97	15361.0
98-99	19683.0
100-101	23857.0
102-103	28391.0
104-105	32698.0
106-107	36984.0
108-109	41004.0
110-111	43922.0
112-113	47900.0
114-115	51349.0
116-117	55287.0
118-119	59450.0
120-121	62629.0
122-123	66422.0
124-125	71083.0
126-127	74537.0
128-129	78771.0
130-131	82655.0
132-133	88509.0
134-135	146283.0
136-137	149682.0
138-139	152702.0
140-141	150736.0
142-143	151139.0
144-145	152259.0
146-147	154520.0
148-149	158061.0
150-151	160720.0
152-153	146219.0
154-155	143649.0
156-157	151332.0
158-159	156124.0
160-161	162050.0
162-163	160726.0
164-165	169316.0
166-167	174004.0
168-169	180522.0
170-171	181144.0
172-173	195140.0
174-175	209043.0
176-177	326800.0
178-179	2007004.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.28983926146738
#Duplication Level	Percentage of total
1	94.77390824377612
2	4.710292711053865
3	0.41501989331755124
4	0.0654308218101036
5	0.019570616736285594
6	0.007264223153914235
7	0.002090995392769215
8	0.002106574086354582
9	0.0021222283844974776
>10	0.002193692288565262
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.4835410760627829E-5	0.0	1.4835410760627829E-5	1.4835410760627829E-5	0.0	0.0
2	2.9670821521255658E-5	1.4835410760627829E-5	1.4835410760627829E-5	2.9670821521255658E-5	0.0	0.0
3	4.450623228188348E-5	1.4835410760627829E-5	1.4835410760627829E-5	2.9670821521255658E-5	1.4835410760627829E-5	0.0
4	5.9341643042511315E-5	1.4835410760627829E-5	1.4835410760627829E-5	2.9670821521255658E-5	1.4835410760627829E-5	0.0
5	7.417705380313914E-5	2.9670821521255658E-5	1.4835410760627829E-5	4.450623228188348E-5	2.9670821521255658E-5	0.0
6	8.901246456376697E-5	2.9670821521255658E-5	2.9670821521255658E-5	7.417705380313914E-5	2.9670821521255658E-5	0.0
7	1.038478753243948E-4	2.9670821521255658E-5	2.9670821521255658E-5	7.417705380313914E-5	2.9670821521255658E-5	0.0
8	1.1868328608502263E-4	2.9670821521255658E-5	2.9670821521255658E-5	8.901246456376697E-5	4.450623228188348E-5	0.0
9	1.3351869684565047E-4	4.450623228188348E-5	5.9341643042511315E-5	8.901246456376697E-5	4.450623228188348E-5	0.0
10-14	1.7209076482328282E-4	8.011121810739027E-5	5.9341643042511315E-5	1.2758453254139933E-4	5.3407478738260174E-5	0.0
15-19	2.789057222998032E-4	1.3351869684565047E-4	6.824288949888803E-5	2.0176158634453847E-4	1.0088079317226923E-4	0.0
20-24	3.738523511678213E-4	1.8989325773603621E-4	1.0978203962864592E-4	2.4330073647429643E-4	1.3351869684565047E-4	0.0
25-29	7.120997165101358E-4	2.343994900179197E-4	1.3351869684565047E-4	2.581361472349242E-4	1.4835410760627828E-4	0.0
30-34	0.0028780696875617988	2.729715579955521E-4	1.4835410760627828E-4	2.907740509083054E-4	1.6022243621478054E-4	0.0
35-39	0.009002127249548967	3.0560946166893326E-4	1.5132118975840387E-4	3.1451070812531E-4	2.3143240786579412E-4	2.6703739369130087E-5
40-44	0.016108289003889695	3.5011569395081675E-4	1.6615660051903168E-4	3.471486117986912E-4	2.6110322938704977E-4	3.8572067977632356E-5
45-49	0.023736657217004527	4.0352317268907694E-4	2.136299149530407E-4	3.946219262327002E-4	3.6198402255931903E-4	5.340747873826019E-5
50-54	0.03079831273906337	4.153915012975793E-4	2.2253116140941742E-4	4.480294049709604E-4	4.5396356927521154E-4	7.417705380313914E-5
55-59	0.037192374776893966	4.3022691205820703E-4	2.25498243561543E-4	4.98469801557095E-4	5.044039658613461E-4	8.901246456376697E-5
60-64	0.04390688168715411	4.777002264922161E-4	2.4923490077854754E-4	5.370418695347275E-4	5.40008951686853E-4	9.791371102014366E-5
65-69	0.050087313810031675	4.895685551007183E-4	2.700044758434265E-4	5.993505947293643E-4	6.052847590336155E-4	1.0384787532439482E-4
70-74	0.07364297901575653	4.895685551007183E-4	2.8187280445192875E-4	6.76494730684629E-4	6.794618128367546E-4	1.0384787532439482E-4
75-79	0.39378520906580083	5.162722944698484E-4	3.0264237951680773E-4	7.506717844877681E-4	7.684742774005215E-4	1.0384787532439482E-4
80-84	0.5037037344734445	5.281406230783507E-4	3.1154362597318443E-4	8.278159204430329E-4	9.613346172886834E-4	1.0384787532439482E-4
85-89	0.5044989124902142	5.756139375123597E-4	3.3824736534231447E-4	8.456184133557863E-4	0.0012194707645236075	1.0384787532439482E-4
90-94	0.5052050780424201	6.112189233378665E-4	3.5604985825506786E-4	8.574867419642886E-4	0.0012461745038927377	1.0384787532439482E-4
95-99	0.5060239927164067	6.408897448591223E-4	3.5604985825506786E-4	9.197954671589254E-4	0.0012610099146533656	1.0384787532439482E-4
100-104	0.5070268664838252	6.705605663803779E-4	3.5604985825506786E-4	9.643016994408087E-4	0.0013025490647831234	1.0384787532439482E-4
105-109	0.5078991886365501	6.824288949888801E-4	3.5901694040719345E-4	0.001017709178179069	0.0013440882149128813	1.0384787532439482E-4
110-114	0.5088961282396643	6.913301414452567E-4	3.768194333199469E-4	0.0011067216427428361	0.0013796932007383881	1.0384787532439482E-4
115-119	0.5101007635934273	7.091326343580103E-4	4.0055609053695135E-4	0.0011423266285683427	0.0013826602828905135	1.0384787532439482E-4
120-124	0.5179397946393431	7.23968045118638E-4	4.0649025484120247E-4	0.0011719974500895985	0.0014182652687160205	1.0384787532439482E-4
125-129	0.5348966691387406	7.536388666398938E-4	4.153915012975793E-4	0.001222437846675733	0.0014538702545415273	1.0384787532439482E-4
130-134	0.5544764442606172	7.744084417047727E-4	4.3319399421033256E-4	0.0012639769968054912	0.001510244815431913	1.0384787532439482E-4
135-139	0.5778036441406285	8.040792632260284E-4	4.4506232281883484E-4	0.0013233186398480025	0.0015458498012574198	1.0384787532439482E-4
140-144	0.5998906036810511	8.307830025951585E-4	4.4506232281883484E-4	0.0013678248721298858	0.0015666193763222986	1.0384787532439482E-4
145-149	0.6243927680933041	8.485854955079117E-4	4.5396356927521154E-4	0.0013856273650426393	0.00162596101936481	1.0384787532439482E-4
150-154	0.6484409689362818	8.663879884206651E-4	4.747331443400905E-4	0.001415298186563895	0.0016437635122775632	1.0384787532439482E-4
155-159	0.6801976492104818	8.752892348770419E-4	4.806673086443417E-4	0.0014360677616287738	0.0016645330873424422	1.0384787532439482E-4
160-164	0.7169182579251877	8.752892348770419E-4	4.955027194049695E-4	0.0014509031723894017	0.0017209076482328281	1.0384787532439482E-4
165-167	0.7314450921419945	8.901246456376697E-4	5.044039658613461E-4	0.0014835410760627827	0.0017357430589934563	1.038478753243948E-4
>>END_MODULE
