##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479259
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7304662
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.30820248767157	39.0	39.0	39.0	37.0	39.0
2	40.02881379042589	41.0	40.0	41.0	38.0	41.0
3	40.0514133302814	41.0	40.0	41.0	38.0	41.0
4	40.07053892431984	41.0	40.0	41.0	38.0	41.0
5	40.079260340861765	41.0	40.0	41.0	38.0	41.0
6	40.06678419891297	41.0	40.0	41.0	38.0	41.0
7	40.0923622475619	41.0	40.0	41.0	38.0	41.0
8	40.079261299153885	41.0	40.0	41.0	38.0	41.0
9	40.06179478256489	41.0	40.0	41.0	38.0	41.0
10-14	39.99903661524654	41.0	40.0	41.0	38.0	41.0
15-19	39.88643534772725	41.0	40.0	41.0	38.0	41.0
20-24	39.73644527289558	41.0	39.8	41.0	37.6	41.0
25-29	39.56626737828527	40.0	39.0	41.0	37.0	41.0
30-34	39.57182369286902	40.2	39.0	41.0	36.8	41.0
35-39	39.548700460062356	40.8	39.0	41.0	36.4	41.0
40-44	39.217318802704355	40.0	38.4	41.0	35.2	41.0
45-49	38.706775152635394	39.8	37.2	41.0	35.0	41.0
50-54	38.03641537418158	38.8	35.8	40.8	34.8	41.0
55-59	37.234548922318375	37.2	35.0	39.6	34.0	41.0
60-64	36.30672512978698	35.8	34.8	38.4	33.8	40.2
65-69	35.72697622422502	35.0	35.0	37.0	33.8	39.0
70-74	35.32916181474242	35.0	35.0	36.0	33.8	37.8
75-79	35.045924972298515	35.0	35.0	35.6	33.0	37.0
80-84	34.92728490380527	35.0	35.0	35.0	33.0	36.2
85-89	34.73938791418412	35.0	34.6	35.0	32.6	36.0
90-94	39.401509526154136	40.6	39.8	40.6	36.8	40.6
95-99	39.691728826290934	41.0	39.8	41.0	37.0	41.0
100-104	39.596889813540926	41.0	39.0	41.0	37.0	41.0
105-109	39.46563352322612	40.4	39.0	41.0	36.0	41.0
110-114	39.30636156051752	40.0	38.8	41.0	35.8	41.0
115-119	39.10071107087381	40.0	38.0	41.0	35.0	41.0
120-124	38.889996286319686	40.0	38.0	41.0	35.0	41.0
125-129	38.55292835403841	39.6	37.6	40.6	34.6	41.0
130-134	38.46298938586462	40.0	37.0	41.0	34.0	41.0
135-139	38.197836370914295	39.0	36.0	41.0	34.0	41.0
140-144	37.730366467793964	38.4	35.2	40.6	34.0	41.0
145-149	36.956634981026866	36.8	35.0	39.4	34.0	41.0
150-154	36.16315841408382	35.8	35.0	38.0	34.0	39.8
155-159	35.486547462969874	35.0	35.0	36.6	33.2	38.6
160-164	35.002435741204536	35.0	35.0	35.8	33.0	37.0
165-169	34.69907841111247	35.0	35.0	35.0	33.0	36.0
170-174	34.538645861622136	35.0	34.2	35.0	33.0	36.0
175-178	34.24410882107104	35.0	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	45.0
30	519.0
31	3504.0
32	17179.0
33	64039.0
34	177763.0
35	408407.0
36	878053.0
37	1953709.0
38	2950695.0
39	839313.0
40	11435.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.85187514494168	29.414694341777896	29.536260541555514	20.197169971724907
2	20.67745229005805	29.982852047089924	29.748015719276268	19.59167994357576
3	18.887937593827065	28.90240506679159	31.621901191321378	20.587756148059967
4	19.560809247573673	27.836277708674267	32.009201794689474	20.593711249062586
5	20.44439564760149	28.69812456757068	30.244794351881033	20.612685432946794
6	20.465916150535097	28.91038627112384	30.02292234739951	20.600775230941554
7	20.511380266465444	29.016441828519923	29.567418725192212	20.90475917982242
8	20.702696442354213	29.110286006388797	29.372132481968364	20.81488506928863
9	20.82337279945328	29.15128721903902	29.242858875605744	20.782481105901958
10-14	20.913038823699168	29.21247827757123	28.941213159486367	20.93326973924324
15-19	20.943041580842483	29.103394517090592	29.059685444720095	20.89387845734683
20-24	20.870318161196234	28.95287420554161	29.20686816173014	20.969939471532015
25-29	20.950351980693974	28.971207702697267	28.985713507346407	21.09272680926236
30-34	21.020386706462258	29.003458339345478	28.954812693592118	21.021342260600147
35-39	20.99064953313377	29.00623464850256	29.00574455053499	20.997371267828683
40-44	20.998220040845148	28.976927337637253	28.990828049264977	21.034024572252623
45-49	21.105901956859878	28.92845966041961	28.92094391225768	21.044694470462836
50-54	21.078199100793437	28.997270510257696	28.922841330646097	21.001689058302766
55-59	21.053738010054403	28.95911679417884	28.900789660082836	21.086355535683925
60-64	21.04029727864205	28.994316232564906	28.915645925848448	21.049740562944596
65-69	21.01849202605131	28.971333649660995	28.96025853078486	21.049915793502834
70-74	20.97978250054554	29.002639684081206	28.93599457442384	21.08158324094941
75-79	20.965008374104098	28.98161749304759	28.96564960842815	21.087724524420157
80-84	20.95787868076579	29.027256839536175	28.956132398733846	21.058732080964184
85-89	20.93780656791512	28.98625015093101	28.929497353881672	21.1464459272722
90-94	20.034290100228766	29.061530870483175	30.46338682325345	20.440792206034605
95-99	20.491186570946454	29.18505718835126	29.426657608569762	20.897098632132522
100-104	20.774623510219257	29.254154991434007	28.99965082432509	20.971570674021645
105-109	20.710340840262102	29.128349209845272	29.145561918396563	21.015748031496063
110-114	20.717947723566645	28.995045988513564	29.198386073701165	21.088620214218626
115-119	20.808046118632834	29.062200093682183	29.019399466209567	21.11035432147542
120-124	20.7991805790254	29.06948052975451	28.987328958601537	21.14400993261855
125-129	20.793374190038552	29.061456507273554	29.042578590701236	21.102590711986657
130-134	20.908402127484745	29.042239883991833	28.957178453176734	21.092179535346688
135-139	20.978923711806765	29.01792554314323	28.870789337855783	21.13236140719422
140-144	20.96888072431872	29.051687483191497	28.925279596817816	21.054152195671968
145-149	20.967326303796373	29.0637903244146	28.927512944450985	21.041370427338045
150-154	20.904740840070055	29.101030130659232	28.945179864810676	21.049049164460033
155-159	20.88899839177877	29.102959548087735	28.99162042137597	21.016421638757528
160-164	20.855270281796532	29.186645415795493	28.988969601388114	20.969114701019862
165-169	20.77490007857489	29.18005792176695	29.032741355811137	21.012300643847023
170-174	20.787117816374874	29.257292141573103	29.03822810016311	20.917361941888906
175-178	20.74176299742443	29.30975197227835	28.99842355492081	20.950061475376405
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	6.5
9	18.0
10	41.5
11	88.0
12	170.0
13	309.5
14	569.5
15	991.0
16	1638.0
17	2633.5
18	4277.5
19	6702.5
20	9916.0
21	14451.0
22	20450.0
23	27535.0
24	36012.5
25	46294.5
26	57707.5
27	70736.5
28	84870.0
29	99741.0
30	116421.0
31	133999.0
32	152494.5
33	172843.0
34	194740.5
35	218185.0
36	243173.5
37	269256.0
38	296364.0
39	322235.5
40	344542.0
41	362095.0
42	374316.0
43	378650.5
44	374539.5
45	361895.5
46	341474.5
47	316032.0
48	286255.5
49	253777.5
50	220621.5
51	189702.0
52	160470.5
53	133031.0
54	109479.5
55	88969.5
56	72192.5
57	58853.5
58	47899.5
59	39568.5
60	33269.0
61	28246.0
62	23795.5
63	20242.5
64	17285.0
65	14487.0
66	11871.0
67	9584.5
68	7547.5
69	5724.5
70	4292.5
71	3103.5
72	2199.5
73	1530.5
74	1013.0
75	662.0
76	446.0
77	297.0
78	187.0
79	116.0
80	73.0
81	50.5
82	26.0
83	15.5
84	11.5
85	5.0
86	1.5
87	1.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1062.0
92-93	2170.0
94-95	3242.0
96-97	4061.0
98-99	5118.0
100-101	5978.0
102-103	6666.0
104-105	7693.0
106-107	8386.0
108-109	9311.0
110-111	10222.0
112-113	10649.0
114-115	12465.0
116-117	11589.0
118-119	12552.0
120-121	13658.0
122-123	13194.0
124-125	14115.0
126-127	16864.0
128-129	15447.0
130-131	17921.0
132-133	17496.0
134-135	82543.0
136-137	73199.0
138-139	68260.0
140-141	64799.0
142-143	62966.0
144-145	68407.0
146-147	67135.0
148-149	68102.0
150-151	68774.0
152-153	73341.0
154-155	65716.0
156-157	64170.0
158-159	65114.0
160-161	66983.0
162-163	69599.0
164-165	72668.0
166-167	75740.0
168-169	86461.0
170-171	79797.0
172-173	81669.0
174-175	92245.0
176-177	204036.0
178-179	5363079.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.20036774337018
#Duplication Level	Percentage of total
1	85.30763140786176
2	12.327407861581383
3	1.832787282700736
4	0.34358787385869294
5	0.10265390303874553
6	0.03307395146751402
7	0.019771674349222612
8	0.011524693298863421
9	0.005274079142395561
>10	0.01628727270068263
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.106966208703428E-5	0.0	0.0
2	1.368988736234476E-5	0.0	0.0	6.84494368117238E-5	0.0	0.0
3	1.368988736234476E-5	0.0	1.368988736234476E-5	9.582921153641333E-5	0.0	0.0
4	1.368988736234476E-5	0.0	1.368988736234476E-5	1.0951909889875809E-4	0.0	0.0
5	1.368988736234476E-5	0.0	1.368988736234476E-5	1.5058876098579237E-4	0.0	0.0
6	1.368988736234476E-5	0.0	4.106966208703428E-5	1.5058876098579237E-4	0.0	0.0
7	2.737977472468952E-5	0.0	4.106966208703428E-5	1.9165842307282665E-4	0.0	0.0
8	2.737977472468952E-5	0.0	5.475954944937904E-5	1.9165842307282665E-4	0.0	0.0
9	2.737977472468952E-5	0.0	6.84494368117238E-5	2.053483104351714E-4	0.0	0.0
10-14	3.559370714209638E-5	0.0	6.84494368117238E-5	2.4367999504973674E-4	1.0951909889875808E-5	0.0
15-19	5.475954944937904E-5	0.0	8.761527911900647E-5	3.2581931922380525E-4	1.368988736234476E-5	0.0
20-24	7.39253917566617E-5	0.0	1.0951909889875809E-4	3.8057886867318434E-4	1.368988736234476E-5	0.0
25-29	8.213932417406856E-5	2.737977472468952E-6	1.368988736234476E-4	4.5176628295737713E-4	1.368988736234476E-5	0.0
30-34	1.0404314395382017E-4	1.368988736234476E-5	1.4237482856838552E-4	5.09263809879225E-4	4.380763955950323E-5	0.0
35-39	1.4511280604085448E-4	1.368988736234476E-5	1.5058876098579237E-4	5.831892016358868E-4	7.940134670159961E-5	8.213932417406856E-6
40-44	1.8070651318295085E-4	1.368988736234476E-5	1.8344449065541983E-4	6.762804356998312E-4	1.1499505384369597E-4	2.737977472468952E-5
45-49	2.080862879076404E-4	2.464179725222057E-5	2.3272808515986096E-4	7.365159400941481E-4	1.9165842307282665E-4	2.737977472468952E-5
50-54	2.683217923019573E-4	2.737977472468952E-5	2.601078598845505E-4	8.405590840479683E-4	2.3546606263232986E-4	3.833168461456533E-5
55-59	3.0117752197158474E-4	3.559370714209638E-5	3.1486740933392947E-4	9.254363856945058E-4	3.1760538680639843E-4	4.106966208703428E-5
60-64	3.1760538680639843E-4	4.106966208703428E-5	3.258193192238053E-4	0.0010103136873410433	3.6962695878330857E-4	5.20215719769101E-5
65-69	3.5867504889343274E-4	4.106966208703428E-5	3.2855729669627426E-4	0.0011116188538223945	4.326004406500944E-4	7.39253917566617E-5
70-74	8.077033543783408E-4	4.6545617031972184E-5	3.5319909394849477E-4	0.0011609024483268354	4.791460576820666E-4	8.213932417406856E-5
75-79	0.007504796252037399	5.475954944937904E-5	3.559370714209638E-4	0.0012375658175559662	4.983118999893493E-4	8.213932417406856E-5
80-84	0.010426218215161768	6.571145933925485E-5	3.559370714209638E-4	0.0013251810966749726	6.625905483374864E-4	8.213932417406856E-5
85-89	0.01045086001241399	9.035325659147541E-5	3.559370714209638E-4	0.001379940646124352	0.0010157896422859814	8.213932417406856E-5
90-94	0.010524785404170654	1.0951909889875809E-4	3.8057886867318434E-4	0.001478507835133234	0.0010322175071207948	8.213932417406856E-5
95-99	0.01055490315636781	1.0951909889875809E-4	3.9700673350799803E-4	0.001566123114252241	0.0010404314395382017	8.213932417406856E-5
100-104	0.010576806976147563	1.0951909889875809E-4	4.2986246317762547E-4	0.0016865941230408744	0.0010404314395382017	8.213932417406856E-5
105-109	0.010595972818454844	1.0951909889875809E-4	4.4629032801243916E-4	0.0017495676049076605	0.0010404314395382017	8.213932417406856E-5
110-114	0.010604186750872251	1.0951909889875809E-4	4.8462201262700446E-4	0.001870038613696294	0.0010404314395382017	8.213932417406856E-5
115-119	0.010628828548124471	1.2047100878863389E-4	4.928359450444114E-4	0.001946701982925425	0.0010513833494280775	8.213932417406856E-5
120-124	0.010708229894826072	1.259469637335718E-4	5.12001787351694E-4	0.0020096754647922106	0.001089715034042643	8.487730164653752E-5
125-129	0.010804059106362486	1.368988736234476E-4	5.503334719662593E-4	0.002102766698856155	0.0011170948087673325	1.0130516648135123E-4
130-134	0.010957385844820746	1.368988736234476E-4	5.667613368010732E-4	0.0021575262483055344	0.0011472125609644907	1.2868494120604076E-4
135-139	0.011099760673389131	1.6975460329307504E-4	5.777132466909488E-4	0.0022506174823694785	0.0011828062681065875	1.5058876098579237E-4
140-144	0.011236659547012579	1.943964005452956E-4	6.297348186678589E-4	0.0023053770318188574	0.0012320898626110284	1.6427864834813713E-4
145-149	0.011354392578328744	2.1356224285257828E-4	6.84494368117238E-4	0.0023710884911581124	0.0012457797499733731	1.6427864834813713E-4
150-154	0.011485815497007253	2.1903819779751617E-4	6.87232345589707E-4	0.002461441747749588	0.001262207614808187	1.6427864834813713E-4
155-159	0.011630928303048108	2.2999010768739195E-4	6.981842554795827E-4	0.0025463190493961252	0.0012978013219502832	1.779685357104819E-4
160-164	0.011784255041506368	2.3272808515986096E-4	7.200880752593344E-4	0.0025901266889556285	0.001366250758762007	1.779685357104819E-4
165-167	0.011896512117877596	2.3272808515986093E-4	7.39253917566617E-4	0.002646711556719987	0.0013826786235968207	1.779685357104819E-4
>>END_MODULE
