##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479260
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7212280
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.207056437076766	39.0	39.0	39.0	37.0	39.0
2	39.957258314985	41.0	40.0	41.0	38.0	41.0
3	40.016766542618974	41.0	40.0	41.0	38.0	41.0
4	40.03875833994243	41.0	40.0	41.0	38.0	41.0
5	40.05704839523701	41.0	40.0	41.0	38.0	41.0
6	40.048026698907975	41.0	40.0	41.0	38.0	41.0
7	40.07564001952226	41.0	40.0	41.0	38.0	41.0
8	40.06039629631684	41.0	40.0	41.0	38.0	41.0
9	40.04001369885806	41.0	40.0	41.0	38.0	41.0
10-14	39.98249305351428	41.0	40.0	41.0	38.0	41.0
15-19	39.864000011092195	41.0	40.0	41.0	38.0	41.0
20-24	39.71070352232581	40.8	39.6	41.0	37.4	41.0
25-29	39.538753514838575	40.0	39.0	41.0	37.0	41.0
30-34	39.52913475350375	40.2	39.0	41.0	36.8	41.0
35-39	39.49659541781517	40.6	39.0	41.0	36.0	41.0
40-44	39.15877373035988	40.0	38.2	41.0	35.0	41.0
45-49	38.660652276395254	39.8	37.2	41.0	35.0	41.0
50-54	37.99447076375293	38.8	35.8	40.8	34.8	41.0
55-59	37.212805132357595	37.2	35.0	39.6	34.0	41.0
60-64	36.31636996899732	35.8	34.8	38.4	33.8	40.2
65-69	35.75679956962292	35.0	35.0	37.0	33.8	39.0
70-74	35.35811174829596	35.0	35.0	36.0	33.6	38.0
75-79	35.08460234488955	35.0	35.0	35.6	33.0	37.0
80-84	34.958253506519426	35.0	35.0	35.0	33.0	36.8
85-89	34.778920840566364	35.0	34.6	35.0	32.6	36.6
90-94	39.33477448495097	40.6	38.8	40.6	36.6	40.6
95-99	39.62962365365694	41.0	39.0	41.0	37.0	41.0
100-104	39.52861324052488	41.0	39.0	41.0	36.4	41.0
105-109	39.386910557296275	40.0	39.0	41.0	36.0	41.0
110-114	39.2209526095107	40.0	38.4	41.0	35.4	41.0
115-119	39.0079135364915	40.0	38.0	41.0	35.0	41.0
120-124	38.78684513460085	40.0	38.0	41.0	35.0	41.0
125-129	38.45677028025865	39.6	37.6	40.6	34.6	41.0
130-134	38.36666680463147	39.8	36.8	41.0	34.0	41.0
135-139	38.107684673567825	39.0	36.0	41.0	34.0	41.0
140-144	37.64745768638293	38.4	35.2	40.6	34.0	41.0
145-149	36.892066565607976	36.8	35.0	39.4	34.0	41.0
150-154	36.113648711201236	35.6	35.0	38.0	33.6	39.8
155-159	35.44246343216663	35.0	35.0	36.6	33.0	38.4
160-164	34.959666049999	35.0	35.0	35.8	33.0	37.0
165-169	34.66254154219936	35.0	34.6	35.0	33.0	36.0
170-174	34.50097552152131	35.0	34.0	35.0	33.0	36.0
175-178	34.20424780600634	34.75	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	43.0
30	454.0
31	3454.0
32	17570.0
33	64269.0
34	181120.0
35	420877.0
36	900507.0
37	1962096.0
38	2851373.0
39	799241.0
40	11274.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.822985241837532	29.417313249069643	29.569359481329066	20.19034202776376
2	20.699889632682037	29.97063341966757	29.75221427897974	19.577262668670656
3	18.90981769981199	28.936896515387645	31.572956124831535	20.58032965996883
4	19.566919198921838	27.878118985951737	31.981301336054617	20.573660479071805
5	20.437711791555515	28.694213203037044	30.28219924905855	20.58587575634889
6	20.47538365121709	28.952855407721273	29.97708075670939	20.594680184352242
7	20.47642354428835	29.05197801527395	29.58304447414687	20.888553966290825
8	20.71519685869101	29.114343869067756	29.37140543628367	20.79905383595756
9	20.833730803573904	29.181312982857015	29.22670778172783	20.75824843184125
10-14	20.908472771439822	29.20623436694083	28.97195339060602	20.91333947101333
15-19	20.92247389175129	29.092184440981217	29.092264859378723	20.893076807888768
20-24	20.864065177724658	28.976595473276134	29.201908411764382	20.95743093723483
25-29	20.95792176676446	28.958950567642965	29.007551010221455	21.075576655371115
30-34	21.025695064528833	28.996020675847305	28.96537849334746	21.012905766276408
35-39	20.976448501722064	29.01231233396374	29.030780834909347	20.98045832940485
40-44	21.001270056071032	28.983586327763206	28.984224128846915	21.03091948731885
45-49	21.08382370068827	28.939137138325187	28.935110672353265	21.041928488633275
50-54	21.07328334451796	28.993924251415642	28.934248254366164	20.998544149700233
55-59	21.053334035838876	28.95710926364478	28.930637745622747	21.0589189548936
60-64	21.025107178312545	28.974182921350806	28.954718896104975	21.04599100423167
65-69	20.99396862018668	29.005587692102914	28.975502892289263	21.024940795421145
70-74	20.97889155717748	28.99669729960567	28.955969540838684	21.068441602378165
75-79	20.953032882805438	28.982948526679497	28.98328961160687	21.080728978908194
80-84	20.948382480990755	29.04045877309256	28.954544194069005	21.056614551847684
85-89	20.920402424753338	28.99868557515792	28.94930867908623	21.131603321002512
90-94	20.054646865482677	29.07017868278538	30.43057282186731	20.444601629864636
95-99	20.492327695066958	29.194332801875117	29.4186570572313	20.894682445826625
100-104	20.7642431171255	29.24513701137141	29.008201810723754	20.982418060779338
105-109	20.713814076463375	29.152572881997337	29.141959635616526	20.991653405922765
110-114	20.726267037903185	29.008587564006156	29.18606734163961	21.07907805645105
115-119	20.807746880639023	29.078863505116953	28.985282394415897	21.12810721982813
120-124	20.79083012267296	29.080822168471563	28.992853434656062	21.135494274199413
125-129	20.781367385673306	29.068359046077507	29.037902222733848	21.112371345515342
130-134	20.880932493821263	29.07090279198518	28.962556867659227	21.085607846534323
135-139	20.986852042406106	29.014539042125815	28.886331074666483	21.112277840801593
140-144	20.979868339892587	29.0497507608194	28.91842527130681	21.051955627981204
145-149	20.96669388580205	29.074491589498123	28.924895444797393	21.03391907990243
150-154	20.895604326873414	29.129444144053323	28.946002765361428	21.028948763711835
155-159	20.869125672738047	29.11163686077409	28.99945212566498	21.019785340822875
160-164	20.878953849580164	29.178314334120852	28.9881204787704	20.95461133752858
165-169	20.789659097182547	29.183460382131365	29.032323112248893	20.994557408437192
170-174	20.764539568664723	29.276739986932967	29.034044479206518	20.924675965195792
175-178	20.717074543349934	29.293551684843788	29.05440647990173	20.934967291904545
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	2.5
8	8.5
9	26.0
10	53.0
11	82.0
12	164.0
13	317.0
14	571.0
15	948.5
16	1539.0
17	2589.0
18	4180.0
19	6425.5
20	9585.5
21	13991.5
22	19919.5
23	27213.5
24	35855.0
25	46000.0
26	57286.0
27	69667.0
28	83441.5
29	98509.5
30	114473.5
31	131772.5
32	150598.5
33	170959.5
34	192469.5
35	215923.5
36	241642.0
37	268122.5
38	294183.0
39	319308.5
40	342045.5
41	359124.5
42	369993.5
43	373569.0
44	368673.5
45	355914.0
46	336681.0
47	311828.0
48	282613.0
49	250839.5
50	217978.0
51	186187.5
52	157123.5
53	130998.0
54	107529.0
55	87402.0
56	70823.0
57	57465.5
58	47107.5
59	38987.0
60	32615.0
61	27553.0
62	23402.0
63	20023.0
64	16939.5
65	14200.0
66	11723.0
67	9363.0
68	7315.0
69	5576.0
70	4132.0
71	3061.5
72	2150.5
73	1448.0
74	986.0
75	682.0
76	442.0
77	269.5
78	185.5
79	121.0
80	76.5
81	47.5
82	23.0
83	11.5
84	3.5
85	3.0
86	2.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1193.0
92-93	2610.0
94-95	3466.0
96-97	4388.0
98-99	5422.0
100-101	7739.0
102-103	7254.0
104-105	8921.0
106-107	9325.0
108-109	10632.0
110-111	11362.0
112-113	12024.0
114-115	13338.0
116-117	13322.0
118-119	13704.0
120-121	14423.0
122-123	15739.0
124-125	16588.0
126-127	15707.0
128-129	17735.0
130-131	18205.0
132-133	19871.0
134-135	94637.0
136-137	80972.0
138-139	72511.0
140-141	71853.0
142-143	82708.0
144-145	81897.0
146-147	76748.0
148-149	81174.0
150-151	76452.0
152-153	71316.0
154-155	78006.0
156-157	77437.0
158-159	83072.0
160-161	75261.0
162-163	73546.0
164-165	76979.0
166-167	80463.0
168-169	87085.0
170-171	86226.0
172-173	89544.0
174-175	96532.0
176-177	209725.0
178-179	5065168.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.3706654415024
#Duplication Level	Percentage of total
1	85.6229474664535
2	12.091418507965205
3	1.7551936136847406
4	0.33043824405575506
5	0.10885863384931793
6	0.03826161954866899
7	0.018742677515247344
8	0.01153299778989079
9	0.007389768846743342
>10	0.015216470290922284
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.3865240950157232E-5	0.0	0.0	0.0
2	4.15957228504717E-5	0.0	1.3865240950157232E-5	0.0	0.0	0.0
3	4.15957228504717E-5	0.0	1.3865240950157232E-5	1.3865240950157232E-5	0.0	0.0
4	5.546096380062893E-5	0.0	2.7730481900314463E-5	1.3865240950157232E-5	0.0	0.0
5	5.546096380062893E-5	1.3865240950157232E-5	2.7730481900314463E-5	8.31914457009434E-5	2.7730481900314463E-5	0.0
6	5.546096380062893E-5	1.3865240950157232E-5	2.7730481900314463E-5	8.31914457009434E-5	2.7730481900314463E-5	0.0
7	5.546096380062893E-5	1.3865240950157232E-5	2.7730481900314463E-5	1.1092192760125785E-4	2.7730481900314463E-5	0.0
8	5.546096380062893E-5	2.7730481900314463E-5	2.7730481900314463E-5	1.1092192760125785E-4	2.7730481900314463E-5	0.0
9	5.546096380062893E-5	2.7730481900314463E-5	2.7730481900314463E-5	1.1092192760125785E-4	2.7730481900314463E-5	0.0
10-14	5.546096380062893E-5	2.7730481900314463E-5	3.050353009034591E-5	1.5251765045172955E-4	3.327657828037736E-5	0.0
15-19	5.546096380062893E-5	2.7730481900314463E-5	4.991486742056603E-5	2.162977588224528E-4	4.714181923053459E-5	0.0
20-24	5.546096380062893E-5	2.7730481900314463E-5	5.546096380062893E-5	2.6621262624301886E-4	6.932620475078616E-5	0.0
25-29	6.378010837072327E-5	2.7730481900314463E-5	6.932620475078616E-5	3.1890054185361634E-4	8.873754208100628E-5	8.31914457009434E-6
30-34	1.0260278303116351E-4	2.7730481900314463E-5	6.932620475078616E-5	3.632693128941194E-4	1.1092192760125785E-4	1.3865240950157232E-5
35-39	1.7747508416201255E-4	3.60496264704088E-5	8.041839751091194E-5	4.520068549751257E-4	1.2478716855141508E-4	2.218438552025157E-5
40-44	2.551204334828931E-4	7.764534932088051E-5	1.1092192760125785E-4	5.40744397056132E-4	1.5251765045172955E-4	2.7730481900314463E-5
45-49	3.4108492737386787E-4	8.31914457009434E-5	1.3310631312150943E-4	6.100706018069182E-4	2.3570909615267294E-4	3.6049626470408806E-5
50-54	4.3814161402496853E-4	8.31914457009434E-5	1.5529069864176102E-4	6.516663246573899E-4	2.8839701176327045E-4	4.15957228504717E-5
55-59	4.7696428868540877E-4	9.428363846106916E-5	2.0243251787229556E-4	6.904889993178303E-4	3.35538830993805E-4	4.15957228504717E-5
60-64	5.296522042960062E-4	9.705668665110062E-5	2.218438552025157E-4	7.764534932088049E-4	3.6049626470408805E-4	5.8234011990660375E-5
65-69	5.851131680966352E-4	9.705668665110062E-5	2.329360479626415E-4	8.346875051994654E-4	3.6326931289411946E-4	9.428363846106916E-5
70-74	0.0010093895411714465	9.982973484113206E-5	2.773048190031446E-4	9.151059027103774E-4	3.9099979479443394E-4	1.2478716855141508E-4
75-79	0.007883776004259402	1.2201412036138363E-4	3.41084927373868E-4	9.56701625560849E-4	4.40914662215E-4	1.2478716855141508E-4
80-84	0.010825980133882767	1.497446022616981E-4	3.6049626470408805E-4	9.982973484113206E-4	6.21162794567044E-4	1.2478716855141508E-4
85-89	0.01091194462777374	1.6360984321185535E-4	3.6049626470408805E-4	0.0010787157459222325	0.001056531360401981	1.2478716855141508E-4
90-94	0.010936902061484025	1.663828914018868E-4	3.743615056542453E-4	0.0011452689024829873	0.0010676235531621068	1.2478716855141508E-4
95-99	0.010961859495194308	1.663828914018868E-4	3.9099979479443394E-4	0.001217368155423805	0.001075942697732201	1.2478716855141508E-4
100-104	0.011000682169854748	1.663828914018868E-4	4.0209198755455976E-4	0.0013116517938848741	0.001081488794112264	1.2478716855141508E-4
105-109	0.01104782398908528	1.663828914018868E-4	4.0209198755455976E-4	0.0013948432395858175	0.0010898079386823586	1.2478716855141508E-4
110-114	0.011078327519175628	1.8302118054207547E-4	4.1595722850471694E-4	0.0014253467696761636	0.0010953540350624213	1.2478716855141508E-4
115-119	0.01108110056736566	1.9411337330220123E-4	4.436877104050314E-4	0.0015113112635671384	0.0010953540350624213	1.2478716855141508E-4
120-124	0.011128242386596193	2.0243251787229556E-4	4.575529513551887E-4	0.0015945027092680816	0.0011119923242026099	1.2478716855141508E-4
125-129	0.011258575651527672	2.218438552025157E-4	4.575529513551887E-4	0.0016832402513490877	0.0011397228061029243	1.2478716855141508E-4
130-134	0.011372270627318963	2.3570909615267294E-4	4.6032599954522007E-4	0.0017359281669596855	0.0011785454807633647	1.2478716855141508E-4
135-139	0.011508149988630503	2.773048190031446E-4	4.8251038506547166E-4	0.001777523889810157	0.0011868646253334592	1.3310631312150943E-4
140-144	0.011635710205371949	3.327657828037736E-4	4.936025778255975E-4	0.0018496231427509748	0.0012145951072337732	1.386524095015723E-4
145-149	0.011813185289533961	3.35538830993805E-4	5.40744397056132E-4	0.0019161762993117296	0.001242325589134088	1.386524095015723E-4
150-154	0.011976795132745816	3.466310237539308E-4	5.546096380062893E-4	0.0019577720221622013	0.0012617369264643081	1.4419850588163522E-4
155-159	0.012162589361477924	3.466310237539308E-4	5.740209753365094E-4	0.00199382164863261	0.0013005596011247482	1.5251765045172955E-4
160-164	0.012284603481839306	3.466310237539308E-4	5.90659264476698E-4	0.002038190419673113	0.001317197890264937	1.5251765045172955E-4
165-167	0.012386281915473793	3.4663102375393087E-4	5.96205360856761E-4	0.0020705426485568136	0.001331063131215094	1.5251765045172955E-4
>>END_MODULE
