##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479261
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7216797
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.24085962235047	39.0	39.0	39.0	37.0	39.0
2	39.989051375561765	41.0	40.0	41.0	38.0	41.0
3	40.04210953973072	41.0	40.0	41.0	38.0	41.0
4	40.06389967737765	41.0	40.0	41.0	38.0	41.0
5	40.07003480907112	41.0	40.0	41.0	38.0	41.0
6	40.06144013750144	41.0	40.0	41.0	38.0	41.0
7	40.089563417122584	41.0	40.0	41.0	38.0	41.0
8	40.06584735582835	41.0	40.0	41.0	38.0	41.0
9	40.05583585072436	41.0	40.0	41.0	38.0	41.0
10-14	39.99417253942435	41.0	40.0	41.0	38.0	41.0
15-19	39.88022553495685	41.0	40.0	41.0	38.0	41.0
20-24	39.726951388545366	40.8	39.6	41.0	37.4	41.0
25-29	39.55051197920629	40.0	39.0	41.0	37.0	41.0
30-34	39.54985495643012	40.2	39.0	41.0	36.8	41.0
35-39	39.51989080474343	40.6	39.0	41.0	36.2	41.0
40-44	39.18228302666681	40.0	38.4	41.0	35.2	41.0
45-49	38.674238169647836	39.8	37.2	41.0	35.0	41.0
50-54	38.01092598281482	38.8	35.8	40.8	34.8	41.0
55-59	37.22426084591267	37.2	35.0	39.6	34.0	41.0
60-64	36.32383366194172	35.8	34.8	38.4	33.8	40.2
65-69	35.7606133025496	35.0	35.0	37.0	33.8	39.0
70-74	35.375532275606474	35.0	35.0	36.0	33.6	38.2
75-79	35.098648694150604	35.0	35.0	35.6	33.0	37.0
80-84	34.97187647096074	35.0	35.0	35.0	33.0	37.0
85-89	34.78261652641747	35.0	34.4	35.0	32.6	37.0
90-94	39.344848795651636	40.6	38.8	40.6	36.6	40.6
95-99	39.6440459686955	41.0	39.2	41.0	37.0	41.0
100-104	39.53730464248906	41.0	39.0	41.0	36.4	41.0
105-109	39.39970982540888	40.0	39.0	41.0	36.0	41.0
110-114	39.22968853181662	40.0	38.4	41.0	35.6	41.0
115-119	39.01271580333296	40.0	38.0	41.0	35.0	41.0
120-124	38.79483348270116	40.0	38.0	41.0	35.0	41.0
125-129	38.463162521951006	39.6	37.6	40.6	34.6	41.0
130-134	38.3726756182527	39.8	36.8	41.0	34.0	41.0
135-139	38.1117726142222	39.0	36.0	41.0	34.0	41.0
140-144	37.64570258180135	38.2	35.2	40.6	34.0	41.0
145-149	36.895893606266725	36.8	35.0	39.4	34.0	41.0
150-154	36.1101861930843	35.6	35.0	38.0	33.8	39.8
155-159	35.441663307653656	35.0	35.0	36.6	33.0	38.4
160-164	34.95890187484164	35.0	35.0	35.8	33.0	37.0
165-169	34.66152046689508	35.0	34.6	35.0	33.0	36.0
170-174	34.49492364392875	35.0	34.0	35.0	33.0	36.0
175-178	34.193318925230116	34.75	33.75	35.0	32.25	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	31.0
30	439.0
31	3129.0
32	15948.0
33	60891.0
34	175350.0
35	413940.0
36	897503.0
37	1972274.0
38	2870722.0
39	795703.0
40	10867.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.83555904371427	29.42930222368732	29.526990990601508	20.2081477419969
2	20.6730077068816	29.963403432298293	29.73271937675398	19.630869484066128
3	18.895376993422428	28.924660067340124	31.59337861380887	20.586584325428582
4	19.56947105481836	27.853353780077228	32.00701086645502	20.570164298649388
5	20.44382016010704	28.714816282070842	30.241948055349212	20.59941550247291
6	20.45576451713967	28.929606860217906	30.013952172965375	20.600676449677053
7	20.483533068756127	29.037147643199607	29.562962627326222	20.916356660718044
8	20.696231305938078	29.134434569795992	29.339747259067977	20.829586865197953
9	20.791661453134957	29.200655082857395	29.207957491391262	20.799725972616383
10-14	20.888815356729584	29.238694118734394	28.945137295672858	20.927353228863165
15-19	20.94162825973905	29.103612031764232	29.067052876781762	20.887706831714954
20-24	20.873573137778436	28.976879909466764	29.20522774854274	20.94431920421206
25-29	20.96989564761209	28.964287065300574	28.98082902983138	21.084988257255954
30-34	21.02598147072725	29.010739806038604	28.950483157555908	21.012795565678235
35-39	20.976460887011232	29.015861191606195	29.03015839298237	20.977519528400205
40-44	20.9837328111072	28.991806198788744	28.989916163638803	21.03454482646526
45-49	21.11171479535866	28.933644662583692	28.91693087667562	21.037709665382025
50-54	21.094297096066303	28.99481030157839	28.90263090398691	21.0082616983684
55-59	21.04567718892467	28.93319570995277	28.933641891271154	21.087485209851405
60-64	21.028251175694702	28.98965566026036	28.9420639100698	21.04002925397514
65-69	21.012812193553458	28.990780813150213	28.968945641674555	21.027461351621778
70-74	20.96068103342799	28.991930907852886	28.971356683581373	21.076031375137752
75-79	20.950834559985545	28.980662751079183	28.97730946290993	21.09119322602534
80-84	20.961418202562715	29.03421005191084	28.944782567668177	21.059589177858264
85-89	20.909184503873394	29.003431854879665	28.948149712400113	21.139233928846828
90-94	20.060592243444443	29.05960755019268	30.417838400819353	20.461961805543524
95-99	20.479036831676133	29.199028351674762	29.412549014602195	20.90938580204691
100-104	20.77610770861141	29.242069102582757	29.0105521721199	20.971271016685932
105-109	20.711924305114273	29.14053742529934	29.152030907510408	20.995507362075983
110-114	20.71932202053894	29.00258752069591	29.20739698843985	21.0706934703253
115-119	20.78738767718954	29.0857408687063	29.000457049300177	21.126414404803988
120-124	20.793061496593097	29.081782487458195	28.99890075836062	21.12625525758809
125-129	20.786404237261102	29.06406774441144	29.04004520566284	21.109482812664616
130-134	20.876668804828398	29.066776612386068	28.95956066395083	21.096993918834702
135-139	20.972355116290096	29.01911749745179	28.884645041230694	21.123882345027422
140-144	20.968081279755463	29.051053330345937	28.921817228876357	21.059048161022243
145-149	20.947867351405623	29.078447882368337	28.93614312366129	21.037541642564754
150-154	20.893019828602128	29.10929280446804	28.961986536804684	21.035700830125148
155-159	20.874130740042567	29.11622119585359	29.000592579784357	21.009055484319482
160-164	20.843789349188842	29.212888776358113	28.990886706622643	20.952435167830398
165-169	20.79089634490292	29.197584129703635	29.027805129206197	20.98371439618725
170-174	20.765325626373745	29.269059601316645	29.061241886827865	20.904372885481745
175-178	20.739541317551367	29.31513183044937	29.01747062092871	20.927856231070553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	1.0
7	1.0
8	4.0
9	16.0
10	44.5
11	81.5
12	166.5
13	306.0
14	520.5
15	914.5
16	1532.5
17	2510.5
18	4074.0
19	6384.0
20	9586.5
21	13999.0
22	19674.5
23	26818.0
24	35495.0
25	45425.5
26	56713.5
27	69744.5
28	83716.5
29	98188.5
30	114029.0
31	131801.5
32	150795.0
33	170713.5
34	192695.5
35	216296.5
36	241446.5
37	268122.5
38	294516.5
39	318927.5
40	341591.0
41	359849.5
42	370442.0
43	373992.5
44	370282.5
45	357544.5
46	337086.0
47	311998.0
48	283113.5
49	251408.5
50	219189.5
51	187275.5
52	157455.0
53	130910.5
54	107373.0
55	87456.5
56	70765.0
57	57546.5
58	47099.5
59	38862.5
60	32628.5
61	27416.5
62	23083.0
63	19622.5
64	16626.5
65	14102.0
66	11631.5
67	9237.0
68	7348.0
69	5670.5
70	4132.5
71	2987.5
72	2187.5
73	1518.0
74	999.5
75	656.0
76	436.5
77	285.0
78	175.5
79	115.5
80	76.0
81	38.0
82	16.5
83	10.0
84	7.5
85	4.0
86	1.5
87	2.0
88	1.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1040.0
92-93	2307.0
94-95	3482.0
96-97	4551.0
98-99	5944.0
100-101	7318.0
102-103	7230.0
104-105	9221.0
106-107	9447.0
108-109	9935.0
110-111	10738.0
112-113	11599.0
114-115	13847.0
116-117	12601.0
118-119	13926.0
120-121	14223.0
122-123	14725.0
124-125	15403.0
126-127	15998.0
128-129	19859.0
130-131	18803.0
132-133	19823.0
134-135	87409.0
136-137	80498.0
138-139	86069.0
140-141	72288.0
142-143	76475.0
144-145	74114.0
146-147	76685.0
148-149	79904.0
150-151	77921.0
152-153	71688.0
154-155	73580.0
156-157	85274.0
158-159	75621.0
160-161	72132.0
162-163	74103.0
164-165	80902.0
166-167	78860.0
168-169	78840.0
170-171	88651.0
172-173	90629.0
174-175	91792.0
176-177	215929.0
178-179	5085413.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.38910120423695
#Duplication Level	Percentage of total
1	85.6645861938711
2	12.035998815600298
3	1.7500859480197652
4	0.3650987824417882
5	0.10063709147291176
6	0.03515673101778823
7	0.021864490323400855
8	0.009435088840672581
9	0.0052777624349517665
>10	0.011859095977343448
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3856562682863325E-5	0.0	0.0	2.771312536572665E-5	0.0	0.0
2	8.313937609717995E-5	0.0	2.771312536572665E-5	4.1569688048589974E-5	0.0	0.0
3	8.313937609717995E-5	0.0	2.771312536572665E-5	4.1569688048589974E-5	0.0	0.0
4	8.313937609717995E-5	0.0	2.771312536572665E-5	4.1569688048589974E-5	0.0	0.0
5	8.313937609717995E-5	0.0	2.771312536572665E-5	4.1569688048589974E-5	1.3856562682863325E-5	0.0
6	8.313937609717995E-5	0.0	2.771312536572665E-5	5.54262507314533E-5	1.3856562682863325E-5	0.0
7	9.699593878004327E-5	0.0	2.771312536572665E-5	6.928281341431663E-5	2.771312536572665E-5	0.0
8	9.699593878004327E-5	0.0	2.771312536572665E-5	1.108525014629066E-4	2.771312536572665E-5	0.0
9	1.108525014629066E-4	0.0	2.771312536572665E-5	1.108525014629066E-4	2.771312536572665E-5	0.0
10-14	1.2470906414576994E-4	5.54262507314533E-6	4.988362565830797E-5	1.6627875219435992E-4	2.7713125365726647E-5	0.0
15-19	1.3856562682863327E-4	1.662787521943599E-5	6.374018834117131E-5	2.715886285841212E-4	2.7713125365726647E-5	0.0
20-24	1.3856562682863327E-4	4.7112313121735306E-5	7.759675102403464E-5	3.7689850497388245E-4	4.988362565830797E-5	0.0
25-29	1.4687956443835127E-4	5.5426250731453294E-5	1.2470906414576994E-4	4.600378810710624E-4	9.699593878004327E-5	0.0
30-34	1.690500647309326E-4	5.5426250731453294E-5	1.3025168921891527E-4	5.29320694485379E-4	1.1362381399947926E-4	0.0
35-39	2.2724762799895853E-4	6.928281341431663E-5	1.607361271212146E-4	6.678863213140122E-4	1.524221895114966E-4	0.0
40-44	2.7713125365726654E-4	8.591068863375262E-5	1.9676319009665923E-4	7.260838845820382E-4	1.7736400234065057E-4	1.108525014629066E-5
45-49	3.408714419984378E-4	1.0808118892633393E-4	2.3833287814524923E-4	7.842814478500642E-4	2.4387550321839456E-4	1.9399187756008656E-5
50-54	4.046116303396091E-4	1.1085250146290659E-4	2.521894408281125E-4	8.535642612643808E-4	3.0484437902299314E-4	2.7713125365726647E-5
55-59	5.016075691196524E-4	1.2193775160919728E-4	2.826738787304119E-4	9.450175749712788E-4	3.325575043887198E-4	3.325575043887198E-5
60-64	5.764330076071143E-4	1.2470906414576994E-4	3.270148793155745E-4	0.0010392422012147494	3.5472800468130115E-4	4.434100058516264E-5
65-69	6.096887580459863E-4	1.3302300175548794E-4	3.5472800468130115E-4	0.0011057537020924934	3.852124425836005E-4	5.5426250731453294E-5
70-74	9.893585755564413E-4	1.524221895114966E-4	3.852124425836004E-4	0.0012027496408725368	4.378673807784811E-4	5.5426250731453294E-5
75-79	0.007759675102403463	1.607361271212146E-4	4.350960682419084E-4	0.0012775750793599986	4.932936315099344E-4	6.374018834117131E-5
80-84	0.010475561388244674	1.8013531487722322E-4	4.5726656853448973E-4	0.0013136021423354432	7.011420717528843E-4	6.928281341431663E-5
85-89	0.010533758951512701	1.9399187756008655E-4	4.628091936076351E-4	0.0013551718303840333	0.001058641388970758	6.928281341431663E-5
90-94	0.010572557327024718	2.1616237785266785E-4	4.905223189733617E-4	0.001416140706188632	0.0010724979516536213	6.928281341431663E-5
95-99	0.010600270452390445	2.2170500292581318E-4	4.988362565830798E-4	0.0014854235196029484	0.0010808118892633395	6.928281341431663E-5
100-104	0.010627983577756172	2.2170500292581318E-4	5.126928192659431E-4	0.0015075940198955298	0.0010808118892633395	6.928281341431663E-5
105-109	0.010664010640731613	2.3279025307210385E-4	5.126928192659431E-4	0.0015824194583829918	0.0010808118892633395	6.928281341431663E-5
110-114	0.010713894266389922	2.3556156560867653E-4	5.237780694122337E-4	0.0016988145849190436	0.0010808118892633395	6.928281341431663E-5
115-119	0.010744378704292222	2.3556156560867653E-4	5.62576444924251E-4	0.0018013531487722323	0.0010946684519462027	6.928281341431663E-5
120-124	0.010810890205169968	2.3556156560867653E-4	6.318592583385676E-4	0.0018567793995036858	0.0011112963271656389	7.20541259508893E-5
125-129	0.010910657456486582	2.3556156560867653E-4	6.374018834117129E-4	0.0018817212123328395	0.0011362381399947928	8.313937609717995E-5
130-134	0.011007653395266628	2.3556156560867653E-4	6.457158210214309E-4	0.0019260622129180023	0.0011778078280433827	9.976725131661594E-5
135-139	0.011171160834924412	2.3556156560867653E-4	6.651150087774396E-4	0.00197594583857631	0.0011944357032628188	1.1916643907262462E-4
140-144	0.011248757585948448	2.3556156560867653E-4	7.011420717528843E-4	0.0020812557149660718	0.0012304627662382633	1.2470906414576994E-4
145-149	0.011401179775459944	2.4110419068182185E-4	7.316265096551835E-4	0.002158852465990106	0.0012526332665308444	1.2470906414576994E-4
150-154	0.011573001152727448	2.494181282915399E-4	7.565683224843375E-4	0.00221427871672156	0.001291431642042862	1.2470906414576994E-4
155-159	0.011717109404629228	2.494181282915399E-4	7.648822600940556E-4	0.002316817280574748	0.0013274587050183066	1.2470906414576994E-4
160-164	0.011880616844287015	2.494181282915399E-4	7.870527603866369E-4	0.0023888714065256372	0.0013551718303840333	1.2470906414576994E-4
165-167	0.012018258700270124	2.494181282915399E-4	7.944429271508306E-4	0.002424898469501082	0.0013579431429206056	1.2470906414576994E-4
>>END_MODULE
