##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479312
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4295970
Total Bases	736.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.293506239568714	39.0	39.0	39.0	37.0	39.0
2	39.98831183644206	41.0	40.0	41.0	38.0	41.0
3	40.01885767358711	41.0	40.0	41.0	38.0	41.0
4	40.02941244934206	41.0	40.0	41.0	38.0	41.0
5	40.02181207038224	41.0	40.0	41.0	38.0	41.0
6	40.006403908779625	41.0	40.0	41.0	38.0	41.0
7	40.03146739851535	41.0	40.0	41.0	38.0	41.0
8	40.01109877396723	41.0	40.0	41.0	38.0	41.0
9	39.99141008899038	41.0	40.0	41.0	38.0	41.0
10-14	39.92510403936713	41.0	40.0	41.0	38.0	41.0
15-19	39.80390901239999	41.0	40.0	41.0	38.0	41.0
20-24	39.62761550941929	40.6	39.2	41.0	37.0	41.0
25-29	39.40629832145011	40.0	39.0	41.0	36.2	41.0
30-34	39.337362411748686	40.0	38.8	41.0	36.0	41.0
35-39	39.20340854335574	40.0	38.2	41.0	35.0	41.0
40-44	38.75102791686162	40.0	37.2	41.0	35.0	41.0
45-49	38.153005258416606	39.2	35.6	41.0	34.8	41.0
50-54	37.48844302916454	37.8	35.0	40.2	34.0	41.0
55-59	36.7819874440464	36.4	35.0	39.2	34.0	41.0
60-64	35.99920264806318	35.4	34.8	38.0	33.2	40.0
65-69	35.56194158711537	35.0	34.8	36.8	33.0	39.0
70-74	35.26551316699139	35.0	35.0	36.0	33.0	38.0
75-79	35.03219924720145	35.0	35.0	35.4	33.0	37.0
80-84	34.917102307511456	35.0	35.0	35.0	33.0	37.0
85-89	34.73433976494249	35.0	34.4	35.0	32.4	37.2
90-94	39.161710057010794	40.2	39.6	40.2	37.0	40.2
95-99	39.683142492461876	41.0	39.8	41.0	37.0	41.0
100-104	39.573934752823426	41.0	39.0	41.0	36.6	41.0
105-109	39.436306265337386	40.4	39.0	41.0	36.0	41.0
110-114	39.2484123509761	40.0	38.6	41.0	35.4	41.0
115-119	39.00867071724696	40.0	38.0	41.0	35.0	41.0
120-124	38.70763920943956	40.0	37.8	41.0	34.8	41.0
125-129	38.39771060344576	39.8	37.0	40.8	34.2	40.8
130-134	38.076107641292694	39.0	36.0	40.6	34.0	41.0
135-139	37.84504283409667	39.0	35.0	41.0	34.0	41.0
140-144	37.47199343494192	37.8	35.0	40.6	34.0	41.0
145-149	36.79100930046178	36.4	35.0	39.4	34.0	41.0
150-154	36.1000308541314	35.4	35.0	38.0	33.4	40.2
155-159	35.491246547075185	35.0	35.0	36.6	33.0	38.8
160-164	35.02287641983878	35.0	35.0	35.8	33.0	37.0
165-169	34.71523122378172	35.0	35.0	35.0	33.0	36.0
170-174	34.52619753947525	35.0	34.2	35.0	33.0	36.0
175-179	34.252250417754496	34.8	33.8	35.0	32.4	35.6
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	17.0
30	228.0
31	1870.0
32	10733.0
33	41430.0
34	121804.0
35	294255.0
36	674748.0
37	1263709.0
38	1451800.0
39	430925.0
40	4451.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.644727500424818	27.956759474577336	28.08522871435322	22.313284310644626
2	21.495354948940516	28.36006769134794	29.582399318430998	20.56217804128055
3	20.925006459542313	27.012851579503582	29.58817217066227	22.473969790291832
4	21.267513506844786	26.92933144318978	29.69948579715408	22.103669252811358
5	21.68904810787785	27.58950830662225	29.000877566649674	21.720566018850224
6	22.499482072733283	27.04532387330452	28.112673040081752	22.34252101388045
7	22.20467088922874	27.839347109034744	28.04768189721995	21.90830010451656
8	21.846311775920224	27.947262201551688	28.619427044416046	21.586998978112046
9	22.670572652974766	27.379194919890036	27.556570460222023	22.39366196691318
10-14	22.152603486523418	27.844477498678994	27.994376124600496	22.00854289019709
15-19	22.216305048685165	27.74411832484864	27.912545944222146	22.127030682244055
20-24	22.052472433466715	27.814570399700184	28.071918565539335	22.06103860129377
25-29	21.97340763552818	28.058249941223984	27.93283938202548	22.035503041222356
30-34	22.140485152363727	28.020456381213087	27.751739420899124	22.087319045524062
35-39	21.97464600544231	28.07257033917835	27.9081418166328	22.044641838746546
40-44	21.951847894654758	28.235071473962808	27.867824961533717	21.945255669848716
45-49	22.21152382349039	28.059711776385775	27.656100950425632	22.072663449698204
50-54	22.099604978619496	28.11994031615677	27.802391543702587	21.978063161521145
55-59	22.038389467337993	28.182054343954917	27.814863697837737	21.96469249086935
60-64	22.137803569391778	28.125461769984426	27.684080661643357	22.05265399898044
65-69	22.04729083303654	28.109516593458522	27.846106932776532	21.997085640728404
70-74	21.98026522531582	28.27007171837792	27.77916512452368	21.970497931782578
75-79	22.109032418755252	28.155210581079476	27.657623307425332	22.07813369273994
80-84	22.039804747239856	28.188288093259494	27.72571968612444	22.04618747337621
85-89	22.068236044478894	28.28951785045054	27.61630551423776	22.025940590832803
90-94	21.259871852529923	27.246922995755146	29.542137149029386	21.95106800268555
95-99	21.86447887091881	27.641234804571614	28.403048878764935	22.091237445744643
100-104	21.88209426730523	27.88211248880721	28.15522009822324	22.080573145664324
105-109	21.929397760317933	27.773538571130647	28.11271988508683	22.184343783464595
110-114	21.819538782575737	27.729531889499437	28.28636504717718	22.164564280747644
115-119	21.72483903537209	27.89287558594357	28.215896640068692	22.166388738615638
120-124	21.847321596758803	27.870829110055745	28.062201485437683	22.219647807747773
125-129	21.733113401123944	27.901885043802245	28.18489462123166	22.180106933842154
130-134	21.754345413734292	28.02152263137576	28.131466443162346	22.092665511727606
135-139	21.963183814193435	27.915141376736457	27.975652476686154	22.146022332383957
140-144	21.842435559523768	28.01579211764629	28.14098730211356	22.000785020716386
145-149	21.731467159227556	28.10774035966092	28.20865666271013	21.952135818401395
150-154	21.800172253808604	28.12388093002129	28.13105683922525	21.944889976944857
155-159	21.669387111952226	28.171108596552198	28.28595476099886	21.873549530496714
160-164	21.56602624804012	28.393008216755145	28.279304953870916	21.761660581333814
165-169	21.61060621982633	28.383081055328535	28.19570511323695	21.810607611608184
170-174	21.534355890009138	28.496577970174187	28.30549285916834	21.663573280648336
175-179	21.482396723493945	28.716446345914886	28.238420987440428	21.56273594315074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	3.0
9	7.0
10	10.5
11	23.0
12	58.5
13	122.5
14	210.0
15	359.5
16	617.0
17	1047.0
18	1779.5
19	2822.5
20	4201.5
21	6257.5
22	9065.0
23	12684.0
24	17255.5
25	22512.5
26	28665.0
27	35987.0
28	44224.0
29	53044.0
30	62742.5
31	73240.5
32	84228.5
33	95979.0
34	107929.5
35	120139.5
36	132498.5
37	144638.5
38	156022.5
39	165777.5
40	173696.0
41	179632.0
42	183273.0
43	183393.5
44	180539.0
45	175409.0
46	167735.5
47	158405.0
48	147415.5
49	135680.0
50	124434.0
51	113371.0
52	102623.0
53	93190.5
54	84927.0
55	77802.5
56	71704.5
57	66054.0
58	61188.0
59	57241.0
60	53231.5
61	48765.0
62	44716.5
63	40493.5
64	35798.5
65	31137.5
66	26715.5
67	22309.0
68	18128.0
69	14406.5
70	11206.0
71	8540.5
72	6219.5
73	4411.5
74	3063.0
75	2059.5
76	1323.5
77	840.5
78	533.5
79	316.0
80	172.5
81	92.0
82	54.5
83	27.0
84	12.5
85	4.5
86	2.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	761.0
92-93	1639.0
94-95	2294.0
96-97	2886.0
98-99	3437.0
100-101	4146.0
102-103	4757.0
104-105	5377.0
106-107	6023.0
108-109	6710.0
110-111	6862.0
112-113	7451.0
114-115	7905.0
116-117	8245.0
118-119	8614.0
120-121	8976.0
122-123	9319.0
124-125	9747.0
126-127	10160.0
128-129	10775.0
130-131	11202.0
132-133	12029.0
134-135	47948.0
136-137	46990.0
138-139	41102.0
140-141	41146.0
142-143	41425.0
144-145	43568.0
146-147	43294.0
148-149	44464.0
150-151	45622.0
152-153	44302.0
154-155	42842.0
156-157	42348.0
158-159	42307.0
160-161	43726.0
162-163	45305.0
164-165	46062.0
166-167	47728.0
168-169	49654.0
170-171	51523.0
172-173	54350.0
174-175	54578.0
176-177	56922.0
178-179	3129449.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.70673613198457
#Duplication Level	Percentage of total
1	91.95290667244036
2	6.749490692030843
3	0.8966694620770627
4	0.19032844210168992
5	0.07953026091311177
6	0.04975416984473347
7	0.019275107793516672
8	0.01299804750971743
9	0.01314711832889419
>10	0.032430147316704225
>50	0.0
>100	0.003469879643353098
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.32776299648275E-5	0.0	0.0	0.0	0.0	0.0
2	4.6555259929655E-5	0.0	4.6555259929655E-5	0.0	0.0	0.0
3	4.6555259929655E-5	2.32776299648275E-5	4.6555259929655E-5	0.0	0.0	0.0
4	6.98328898944825E-5	2.32776299648275E-5	4.6555259929655E-5	0.0	0.0	0.0
5	6.98328898944825E-5	2.32776299648275E-5	9.311051985931E-5	2.32776299648275E-5	0.0	0.0
6	6.98328898944825E-5	2.32776299648275E-5	1.39665779788965E-4	6.98328898944825E-5	0.0	0.0
7	6.98328898944825E-5	2.32776299648275E-5	1.39665779788965E-4	6.98328898944825E-5	0.0	0.0
8	6.98328898944825E-5	2.32776299648275E-5	1.629434097537925E-4	6.98328898944825E-5	0.0	0.0
9	6.98328898944825E-5	2.32776299648275E-5	1.8622103971862E-4	6.98328898944825E-5	0.0	0.0
10-14	8.3799467873379E-5	5.5866311915586E-5	1.95532091704551E-4	7.914394188041349E-5	0.0	0.0
15-19	9.77660458522755E-5	8.3799467873379E-5	2.3277629964827502E-4	1.3035472780303402E-4	0.0	0.0
20-24	1.3501025379599954E-4	9.77660458522755E-5	2.60709455606068E-4	1.629434097537925E-4	0.0	0.0
25-29	1.8622103971862E-4	1.58287883760827E-4	2.7933155957793E-4	1.8622103971862E-4	0.0	0.0
30-34	5.86596275113653E-4	2.14154195676413E-4	2.839870855708955E-4	2.60709455606068E-4	9.311051985931E-6	0.0
35-39	0.001792377507291718	2.88642611563861E-4	3.0260918954275754E-4	2.7933155957793E-4	3.7244207943724E-5	1.8622103971862E-5
40-44	0.0027933155957793	3.5381997546537797E-4	3.0260918954275754E-4	2.839870855708955E-4	8.845499386634449E-5	3.25886819507585E-5
45-49	0.003957197094020675	4.3761944333875697E-4	3.119202415286885E-4	3.0260918954275754E-4	1.3501025379599954E-4	6.98328898944825E-5
50-54	0.005456276463755566	4.702081252895156E-4	3.35197871493516E-4	3.25886819507585E-4	1.8622103971862E-4	8.845499386634449E-5
55-59	0.006694646377884389	5.21418911212136E-4	3.6313102745130896E-4	3.7244207943724E-4	2.746760335849645E-4	9.311051985931E-5
60-64	0.0075745407905548675	5.5866311915586E-4	3.7244207943724E-4	4.28308391352826E-4	3.35197871493516E-4	9.311051985931E-5
65-69	0.008342702579394175	6.284960090503425E-4	3.9106418340910203E-4	4.748636512824811E-4	3.770976054302055E-4	9.311051985931E-5
70-74	0.015232881048983116	6.797067949729631E-4	4.00375235395033E-4	5.074523332332395E-4	3.9571970940206747E-4	1.0242157184524101E-4
75-79	0.14410715158625412	6.983288989448251E-4	4.2365286535986054E-4	5.679741711417911E-4	4.143418133739295E-4	1.3035472780303402E-4
80-84	0.1996103324743888	7.122954769237216E-4	4.6555259929655004E-4	6.052183790855151E-4	5.214189112121361E-4	1.39665779788965E-4
85-89	0.20007122954769238	7.216065289096526E-4	4.934857552543429E-4	6.936733729518595E-4	7.867838928111695E-4	1.39665779788965E-4
90-94	0.20037849426322812	7.26262054902618E-4	5.12107859226205E-4	7.216065289096526E-4	7.914394188041349E-4	1.39665779788965E-4
95-99	0.20074628081667237	7.588507368533765E-4	5.5866311915586E-4	7.681617888393075E-4	7.914394188041349E-4	1.39665779788965E-4
100-104	0.20120717788997594	7.960949447971006E-4	6.005628530925495E-4	7.960949447971006E-4	7.914394188041349E-4	1.39665779788965E-4
105-109	0.20157496444342024	8.56616782705652E-4	6.23840483057377E-4	8.519612567126865E-4	7.914394188041349E-4	1.39665779788965E-4
110-114	0.2019706841528223	8.84549938663445E-4	6.284960090503425E-4	9.031720426353071E-4	8.240281007548936E-4	1.39665779788965E-4
115-119	0.2024455478041048	9.264496726001344E-4	6.5177363901517E-4	9.078275686282726E-4	8.379946787337901E-4	1.39665779788965E-4
120-124	0.20345579694457827	0.0010149046664664789	6.70395742987032E-4	9.264496726001346E-4	8.473057307197211E-4	1.39665779788965E-4
125-129	0.205788215467054	0.0010474933484172376	6.983288989448251E-4	9.543828285579276E-4	9.031720426353071E-4	1.67598935746758E-4
130-134	0.20815322267148048	0.001070770978382065	7.54195210860411E-4	9.776604585227551E-4	9.078275686282726E-4	1.8622103971862E-4
135-139	0.2107323840715834	0.0011033596603328235	7.681617888393075E-4	9.823159845157206E-4	9.171386206142035E-4	1.8622103971862E-4
140-144	0.21328361231572845	0.0011592259722484097	7.821283668182041E-4	0.0010009380884875825	9.311051985931001E-4	1.8622103971862E-4
145-149	0.21597450633966248	0.0012104367581710301	7.914394188041349E-4	0.0010288712444453756	9.497273025649622E-4	1.8622103971862E-4
150-154	0.21812535934841257	0.001256992018100685	7.914394188041349E-4	0.0010474933484172376	9.823159845157206E-4	1.8622103971862E-4
155-159	0.22072314285248731	0.0012942362260444091	8.147170487689626E-4	0.0010754265043750304	0.00102421571845241	1.8622103971862E-4
160-164	0.22381441211181646	0.001312858330016271	8.985165166423415E-4	0.0010940486083468926	0.0010381822964313065	1.8622103971862E-4
165-168	0.2261538139232816	0.0013326443154863745	9.194663836106863E-4	0.0011231456458029268	0.0010474933484172376	1.8622103971862E-4
>>END_MODULE
