##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479313
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9200289
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.23372417975131	39.0	39.0	39.0	37.0	39.0
2	39.938520518214155	41.0	40.0	41.0	38.0	41.0
3	39.97626998456244	41.0	40.0	41.0	38.0	41.0
4	39.98928055412173	41.0	40.0	41.0	38.0	41.0
5	39.98400485028242	41.0	40.0	41.0	38.0	41.0
6	39.971225686497455	41.0	40.0	41.0	38.0	41.0
7	40.002679481046734	41.0	40.0	41.0	38.0	41.0
8	39.98066886811925	41.0	40.0	41.0	38.0	41.0
9	39.9648528432096	41.0	40.0	41.0	38.0	41.0
10-14	39.90479212120401	41.0	40.0	41.0	38.0	41.0
15-19	39.78408871721312	41.0	40.0	41.0	37.8	41.0
20-24	39.608201220635564	40.4	39.0	41.0	37.0	41.0
25-29	39.39913933138404	40.0	39.0	41.0	36.2	41.0
30-34	39.32062931936159	40.0	38.8	41.0	36.0	41.0
35-39	39.17794327982523	40.0	38.2	41.0	35.0	41.0
40-44	38.72806923782503	40.0	37.2	41.0	35.0	41.0
45-49	38.14156911810053	39.2	35.6	41.0	34.6	41.0
50-54	37.47840442838263	37.8	35.0	40.2	34.0	41.0
55-59	36.77626035443017	36.4	35.0	39.2	34.0	41.0
60-64	36.007836819039056	35.4	34.8	38.0	33.0	40.0
65-69	35.558087990496816	35.0	35.0	36.8	33.0	39.0
70-74	35.24998106037756	35.0	35.0	36.0	33.0	38.0
75-79	35.016300053183116	35.0	35.0	35.4	33.0	37.0
80-84	34.91396659387548	35.0	35.0	35.0	33.0	37.0
85-89	34.72859885162303	35.0	34.0	35.0	32.4	37.2
90-94	39.1089990984319	40.2	39.6	40.2	36.8	40.2
95-99	39.63786983003356	41.0	39.2	41.0	37.0	41.0
100-104	39.528434452481235	41.0	39.0	41.0	36.2	41.0
105-109	39.39174599790638	40.2	39.0	41.0	36.0	41.0
110-114	39.20532226725696	40.0	38.4	41.0	35.2	41.0
115-119	38.971856326218116	40.0	38.0	41.0	35.0	41.0
120-124	38.67036804325589	40.0	37.8	41.0	34.6	41.0
125-129	38.36955213698154	39.8	37.0	40.8	34.0	41.0
130-134	38.03830836547489	39.0	36.0	40.6	34.0	41.0
135-139	37.78902580679452	39.0	35.0	41.0	34.0	41.0
140-144	37.42468350254416	37.8	35.0	40.4	34.0	41.0
145-149	36.75877942301083	36.4	35.0	39.4	34.0	41.0
150-154	36.07121077292545	35.4	35.0	38.0	33.2	40.2
155-159	35.46352080607457	35.0	35.0	36.6	33.0	38.8
160-164	35.005484684046344	35.0	35.0	35.8	33.0	37.0
165-169	34.69622439003239	35.0	35.0	35.0	33.0	36.0
170-174	34.51945453430104	35.0	34.0	35.0	33.0	36.0
175-179	34.22966423001526	34.8	33.8	35.0	32.4	35.6
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	61.0
30	687.0
31	5281.0
32	26754.0
33	99438.0
34	281236.0
35	655612.0
36	1454320.0
37	2677890.0
38	3060892.0
39	927803.0
40	10313.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.609201623992462	27.95530662134635	28.153006932717005	22.28248482194418
2	21.482412128575525	28.42550924215533	29.55121301080868	20.54086561846046
3	20.88420265928603	27.052813232279988	29.59430948310428	22.468674625329704
4	21.26549502955831	26.95426198024866	29.703175628504713	22.077067361688314
5	21.673264828963525	27.64167516911697	29.008980043996445	21.67607995792306
6	22.49156521061458	27.05499794626017	28.155039477564237	22.29839736556102
7	22.166901496246478	27.850375134955	28.084943853394172	21.897779515404352
8	21.811380055561298	28.007446287828568	28.613514205912445	21.56765945069769
9	22.634647672480725	27.437398977358214	27.58611169714343	22.34184165301764
10-14	22.145667380665977	27.88360887359082	28.016243837557713	21.954479908185494
15-19	22.214269573488398	27.761080113896426	27.93959624529186	22.08505406732332
20-24	22.031620963211047	27.841812360459546	28.104797577554358	22.02176909877505
25-29	21.945714966127696	28.09939774717946	27.965575863975577	21.989311422717265
30-34	22.11336187374114	28.055299132451168	27.78027733694018	22.051061656867518
35-39	21.968875108162365	28.109075704034947	27.925979281737778	21.996069906064907
40-44	21.94276723263802	28.274816149797033	27.897612781511537	21.88480383605341
45-49	22.184809629349687	28.088272009716214	27.686754187830402	22.040164173103694
50-54	22.08388236499962	28.14599410953286	27.835669075177965	21.93445445028955
55-59	22.0014197380104	28.223728624176914	27.85495107816722	21.919900559645463
60-64	22.093277722036774	28.12982070454526	27.757245451746137	22.01965612167183
65-69	22.024892913689996	28.11153214860968	27.887013114479338	21.97656182322099
70-74	21.957758066078142	28.268738079858142	27.85248811205822	21.921015742005494
75-79	22.07749560910532	28.17636924231402	27.691056226603315	22.05507892197734
80-84	22.025086385873312	28.211907256391623	27.751121731067364	22.011884626667705
85-89	22.037670773168102	28.30905420471031	27.657570321975754	21.995704700145833
90-94	21.241591043643382	27.277198913766398	29.547520371663545	21.93368967092667
95-99	21.82872579641357	27.69019757702289	28.41516356957988	22.065913056983657
100-104	21.825807415175028	27.91754366040577	28.198474033621356	22.058174890797847
105-109	21.901235017008087	27.795063000648295	28.152673734656787	22.15102824768683
110-114	21.766090102265327	27.773459358939494	28.31177438449896	22.148676154296215
115-119	21.702913215580672	27.91176626032566	28.25024051211762	22.135080011976044
120-124	21.796801278852833	27.907465779239242	28.10887116265252	22.186861779255405
125-129	21.67741708775456	27.926258830466494	28.229461314614014	22.16686276716493
130-134	21.703389294766474	28.05651770868024	28.180150393060156	22.05994260349313
135-139	21.936114631730856	27.939987936976195	28.006417424002528	22.117480007290418
140-144	21.81150564768212	28.033813754432312	28.169565399208686	21.98511519867688
145-149	21.697966781140565	28.16713609520109	28.216399769806888	21.918497353851464
150-154	21.771094399331385	28.144512327038417	28.16180376611844	21.922589507511763
155-159	21.637651466683877	28.199972413275525	28.30231699620569	21.860059123834912
160-164	21.525583137417968	28.40952039641973	28.315365604612314	21.74953086154999
165-169	21.589580257046702	28.396882982125316	28.22927621985899	21.78426054096899
170-174	21.489948305243814	28.547529109322344	28.300294559128336	21.662228026305506
175-179	21.44078917627865	28.727909256264223	28.272259688989347	21.55904187846778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	8.0
9	15.0
10	27.0
11	62.0
12	116.5
13	251.5
14	481.5
15	814.0
16	1430.0
17	2402.5
18	3846.0
19	6102.0
20	9359.5
21	13919.0
22	19873.0
23	27663.0
24	37511.0
25	48910.5
26	62303.5
27	78299.5
28	95991.5
29	114878.0
30	135723.5
31	158521.5
32	182184.5
33	206841.5
34	233329.5
35	259528.0
36	285277.0
37	311197.5
38	334918.0
39	355141.0
40	371736.5
41	384104.5
42	391280.5
43	391777.5
44	386499.5
45	374969.0
46	357833.5
47	338040.0
48	315296.5
49	290496.5
50	265561.5
51	241663.5
52	218831.5
53	198624.5
54	181019.5
55	165387.5
56	152692.0
57	141623.5
58	131170.0
59	121593.0
60	112741.5
61	104328.0
62	95270.5
63	85537.0
64	75796.0
65	65961.5
66	56504.0
67	47208.5
68	38311.5
69	30533.5
70	23716.5
71	17976.0
72	13245.5
73	9332.0
74	6382.0
75	4244.0
76	2841.0
77	1837.5
78	1056.5
79	613.0
80	367.5
81	208.0
82	108.0
83	56.5
84	31.0
85	14.5
86	7.0
87	3.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1571.0
92-93	3644.0
94-95	5071.0
96-97	6394.0
98-99	7839.0
100-101	9141.0
102-103	10604.0
104-105	11819.0
106-107	13238.0
108-109	14306.0
110-111	15096.0
112-113	16052.0
114-115	16808.0
116-117	18392.0
118-119	18723.0
120-121	19809.0
122-123	20624.0
124-125	21357.0
126-127	22363.0
128-129	23377.0
130-131	24155.0
132-133	26107.0
134-135	110760.0
136-137	102940.0
138-139	89170.0
140-141	91944.0
142-143	90911.0
144-145	97654.0
146-147	93066.0
148-149	98695.0
150-151	101601.0
152-153	94085.0
154-155	94484.0
156-157	94920.0
158-159	98109.0
160-161	94513.0
162-163	95914.0
164-165	98332.0
166-167	102302.0
168-169	105565.0
170-171	105347.0
172-173	115059.0
174-175	119488.0
176-177	123131.0
178-179	6655809.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.81170203170062
#Duplication Level	Percentage of total
1	85.17133453922763
2	11.203451149141614
3	2.305241797738785
4	0.674126760963198
5	0.2786728824943397
6	0.11494876774097613
7	0.07532845366497111
8	0.042536877320856675
9	0.034422063763055656
>10	0.0952500881914536
>50	0.0013158408506998309
>100	0.0033707789024191395
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.0869223781992066E-5	0.0	0.0	0.0
2	8.695379025593653E-5	0.0	1.0869223781992066E-5	1.0869223781992066E-5	1.0869223781992066E-5	0.0
3	1.0869223781992065E-4	3.2607671345976195E-5	2.1738447563984132E-5	2.1738447563984132E-5	1.0869223781992066E-5	0.0
4	1.1956146160191273E-4	4.3476895127968264E-5	3.2607671345976195E-5	2.1738447563984132E-5	2.1738447563984132E-5	0.0
5	1.3043068538390478E-4	7.608456647394447E-5	3.2607671345976195E-5	2.1738447563984132E-5	2.1738447563984132E-5	0.0
6	1.4129990916589684E-4	8.695379025593653E-5	4.3476895127968264E-5	3.2607671345976195E-5	2.1738447563984132E-5	0.0
7	1.5216913294788893E-4	9.782301403792859E-5	4.3476895127968264E-5	3.2607671345976195E-5	2.1738447563984132E-5	0.0
8	1.5216913294788893E-4	1.1956146160191273E-4	4.3476895127968264E-5	3.2607671345976195E-5	3.2607671345976195E-5	0.0
9	1.5216913294788893E-4	1.1956146160191273E-4	6.521534269195239E-5	4.3476895127968264E-5	3.2607671345976195E-5	0.0
10-14	1.7390758051187306E-4	1.3477837489670163E-4	6.956303220474923E-5	7.391072171754605E-5	3.2607671345976195E-5	0.0
15-19	1.8695064905026353E-4	1.6738604624267782E-4	8.695379025593653E-5	1.1521377208911589E-4	3.2607671345976195E-5	0.0
20-24	2.0434140710145083E-4	2.0651525185784924E-4	1.0651839306352223E-4	1.5869066721708414E-4	3.2607671345976195E-5	0.0
25-29	2.521659917422159E-4	2.543398364986143E-4	1.2173530635831114E-4	1.99993717588654E-4	5.2172274153561914E-5	0.0
30-34	6.217196003299462E-4	3.2825055821616045E-4	1.4129990916589684E-4	2.2173216515263813E-4	7.173687696114764E-5	8.695379025593653E-6
35-39	0.001867332645746237	3.9129205615171436E-4	1.7608142526827144E-4	2.3912292320382543E-4	9.782301403792859E-5	1.7390758051187307E-5
40-44	0.0029803411610222243	4.2389972749769055E-4	2.2173216515263813E-4	2.782521288189969E-4	1.2390915111470954E-4	2.1738447563984132E-5
45-49	0.004160738863746563	4.7824584640765086E-4	2.4347061271662228E-4	2.956428868701842E-4	1.7390758051187306E-4	3.4781516102374614E-5
50-54	0.0054237426672140415	5.325919653176112E-4	2.652090602806064E-4	3.2172902394696516E-4	2.0868909661424766E-4	4.99984293971635E-5
55-59	0.006486752753092864	5.738950156891811E-4	2.7390443930620003E-4	3.5433669529294135E-4	2.717305945498016E-4	6.521534269195239E-5
60-64	0.007362812189921425	6.260672898427429E-4	3.1738133443416834E-4	4.0650896944650325E-4	2.956428868701842E-4	7.173687696114764E-5
65-69	0.007923664137072215	6.782395639963049E-4	3.5651054004933976E-4	4.4129048554887785E-4	3.521628505365429E-4	8.043225598674129E-5
70-74	0.014582150625920557	7.347595276626636E-4	3.847705218825191E-4	4.717243121384556E-4	4.173781932284953E-4	8.695379025593653E-5
75-79	0.13816087733765756	7.564979752266478E-4	4.021612799337064E-4	4.8476738067684615E-4	4.6085508835646356E-4	9.564916928153018E-5
80-84	0.1913657277505087	7.956271808418193E-4	4.173781932284953E-4	4.912889149460413E-4	6.26067289842743E-4	1.0869223781992065E-4
85-89	0.19172658598007086	8.347563864569907E-4	4.456381750616746E-4	5.173750520228223E-4	9.195363319565288E-4	1.0869223781992065E-4
90-94	0.19204614115926139	8.89102505366951E-4	4.760720016512525E-4	5.782427052019778E-4	9.391009347641144E-4	1.0869223781992065E-4
95-99	0.1924743885762719	9.260578662257241E-4	4.825935359204477E-4	6.608488059451176E-4	9.456224690333097E-4	1.0869223781992065E-4
100-104	0.1929743728702435	9.93447053674075E-4	4.99984293971635E-4	7.195426143678747E-4	9.543178480589034E-4	1.0869223781992065E-4
105-109	0.1935634847992275	0.001010837811725262	5.152012072664239E-4	7.586718199830462E-4	9.673609165972938E-4	1.0869223781992065E-4
110-114	0.19406346909319913	0.0010825746886864098	5.369396548304081E-4	7.78236422790632E-4	9.717086061100907E-4	1.1956146160191272E-4
115-119	0.19463301641937553	0.0011130085152759876	5.673734814199858E-4	7.999748703546161E-4	0.0010108378117252622	1.3043068538390478E-4
120-124	0.19570037419476716	0.0011325731180835733	5.934596184967668E-4	8.282348521877954E-4	0.0010412716383148397	1.3043068538390478E-4
125-129	0.19787639279592198	0.001152137720891159	6.543272716759223E-4	8.717117473157636E-4	0.0010586623963660274	1.3043068538390478E-4
130-134	0.20032631583638297	0.0012195269083395098	6.891087877782968E-4	8.912763501233492E-4	0.0010738793096608163	1.3043068538390478E-4
135-139	0.2027588481187928	0.0012673514929802748	7.021518563166874E-4	9.064932634181382E-4	0.0011064869810067921	1.3043068538390478E-4
140-144	0.20517833733266425	0.001304306853839048	7.064995458294842E-4	9.521440033025049E-4	0.001130399273327175	1.4129990916589684E-4
145-149	0.20745000510310058	0.0013325668356722273	7.304118381498668E-4	9.912732089176763E-4	0.001152137720891159	1.4129990916589684E-4
150-154	0.2094868976398459	0.0013651745070182035	7.608456647394447E-4	0.0010151855012380591	0.001152137720891159	1.4129990916589684E-4
155-159	0.21214333593216472	0.0013912606440949844	8.086702493802096E-4	0.0010869223781992065	0.0011608330999167524	1.4129990916589684E-4
160-164	0.2152128047281993	0.0014043037126333748	8.347563864569906E-4	0.0011086608257631907	0.001199962305531924	1.4129990916589684E-4
165-168	0.2173953448636233	0.0014374548451684508	8.477994549953811E-4	0.0011276819673816769	0.0012173530635831114	1.4129990916589684E-4
>>END_MODULE
