##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479314
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9158300
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.22897393621087	39.0	39.0	39.0	37.0	39.0
2	39.914390006879	41.0	40.0	41.0	38.0	41.0
3	39.9429182271819	41.0	40.0	41.0	38.0	41.0
4	39.95707205485734	41.0	40.0	41.0	38.0	41.0
5	39.95569144928644	41.0	40.0	41.0	38.0	41.0
6	39.93823460685935	41.0	40.0	41.0	38.0	41.0
7	39.96654455521221	41.0	40.0	41.0	38.0	41.0
8	39.9402422938755	41.0	40.0	41.0	38.0	41.0
9	39.924386185209045	41.0	40.0	41.0	38.0	41.0
10-14	39.8628101285173	41.0	40.0	41.0	38.0	41.0
15-19	39.733171745848026	41.0	39.8	41.0	37.4	41.0
20-24	39.55384270006442	40.0	39.0	41.0	37.0	41.0
25-29	39.32754902110654	40.0	38.8	41.0	36.0	41.0
30-34	39.26638740814344	40.0	38.6	41.0	35.4	41.0
35-39	39.15118458665909	40.0	38.2	41.0	35.0	41.0
40-44	38.6973431750434	40.0	37.2	41.0	35.0	41.0
45-49	38.11074603365254	39.2	35.6	41.0	34.4	41.0
50-54	37.45925861786576	37.8	35.0	40.2	34.0	41.0
55-59	36.7555424478342	36.4	35.0	39.2	34.0	41.0
60-64	35.9893545090246	35.4	34.8	38.0	32.8	40.0
65-69	35.54164882128779	35.0	34.8	36.8	33.0	39.0
70-74	35.23415489774303	35.0	35.0	36.0	33.0	38.2
75-79	35.009604883002304	35.0	35.0	35.4	33.0	37.0
80-84	34.91473939486586	35.0	34.6	35.0	33.0	37.0
85-89	34.72861020058308	35.0	33.8	35.0	32.4	37.2
90-94	39.10434669360273	40.2	39.4	40.2	36.8	40.2
95-99	39.627101730432805	41.0	39.0	41.0	37.0	41.0
100-104	39.516665121159036	41.0	39.0	41.0	36.2	41.0
105-109	39.375841127992246	40.0	39.0	41.0	36.0	41.0
110-114	39.19526163035526	40.0	38.4	41.0	35.0	41.0
115-119	38.95899655087573	40.0	38.0	41.0	35.0	41.0
120-124	38.65981712617715	40.0	37.8	41.0	34.4	41.0
125-129	38.369058148618016	39.8	37.0	40.8	33.8	41.0
130-134	38.029364330569	39.0	35.8	40.6	34.0	41.0
135-139	37.78126856550908	39.0	35.0	41.0	34.0	41.0
140-144	37.42433183238595	37.8	35.0	40.4	34.0	41.0
145-149	36.75922549057409	36.4	35.0	39.4	34.0	41.0
150-154	36.074713956057174	35.4	35.0	38.0	33.0	40.2
155-159	35.46668338939473	35.0	35.0	36.6	33.0	38.8
160-164	35.00255807324735	35.0	35.0	35.8	33.0	37.0
165-169	34.691307902566976	35.0	34.8	35.0	33.0	36.0
170-174	34.511675383477154	35.0	34.0	35.0	33.0	36.0
175-179	34.234695675841415	34.8	33.8	35.0	32.4	35.6
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	87.0
30	823.0
31	5423.0
32	27853.0
33	102669.0
34	287579.0
35	669267.0
36	1467692.0
37	2662484.0
38	3017708.0
39	906717.0
40	9996.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.61595492613258	28.004727951694093	28.136127883995936	22.24318923817739
2	21.42071126737495	28.47853859340707	29.61034253081904	20.49040760839894
3	20.84381380823952	27.119268859941254	29.61446993437647	22.422447397442756
4	21.217660482840703	26.986733345708263	29.741917168033368	22.053689003417666
5	21.63537992858937	27.654368168764947	29.08219866132361	21.62805324132208
6	22.464584038522435	27.09108677374622	28.161350905735784	22.282978281995565
7	22.116484500398546	27.875271611543628	28.142308070275053	21.865935817782773
8	21.764519616085956	28.064040269482327	28.66203334680017	21.509406767631546
9	22.577629035956452	27.469060851904832	27.616828450694996	22.33648166144372
10-14	22.097893713898866	27.921498531386828	28.04841509887206	21.932192655842243
15-19	22.162460281930052	27.814607514495048	27.96474891628359	22.05818328729131
20-24	21.9854448969787	27.874194992520447	28.1360427153511	22.004317395149755
25-29	21.90634943166308	28.127119661945994	27.99005492285686	21.976475983534062
30-34	22.07477807016586	28.086778113842087	27.815596781061984	22.022847034930063
35-39	21.929709662273567	28.15449155410939	27.945400347226013	21.970398436391033
40-44	21.905220401166154	28.310011683391025	27.914257012764377	21.870510902678443
45-49	22.172829018485963	28.111710688664925	27.701001277529674	22.01445901531944
50-54	22.042944651299916	28.158188746819825	27.865357107760175	21.933509494120088
55-59	21.98379830317854	28.248821287793586	27.872710000764332	21.894670408263543
60-64	22.08654444602164	28.156297566142186	27.77080025768975	21.986357730146423
65-69	21.986842536278566	28.149187076204097	27.91058384197941	21.953386545537928
70-74	21.91399713920706	28.319078868348928	27.86401624755686	21.902907744887152
75-79	22.048581068538923	28.202341045827282	27.707810401493727	22.04126748414007
80-84	21.986632890383586	28.252739045456032	27.76835438891497	21.992273675245404
85-89	22.00120983151895	28.341709705949796	27.694794885513684	21.962285577017568
90-94	21.217072318412583	27.31752988975501	29.562387853212822	21.903009938619586
95-99	21.80225743512714	27.720184656686865	28.447489189697684	22.030068718488316
100-104	21.792725267570813	27.93732367826011	28.219243351343952	22.05070770282512
105-109	21.872711935240815	27.828084608498422	28.184154196217705	22.115049260043058
110-114	21.74668043805598	27.789464975517912	28.35529530694251	22.108559279483597
115-119	21.65848525294962	27.954168104727778	28.266664553959604	22.120682088362997
120-124	21.766861107061107	27.932202339934324	28.129556952684474	22.17137960032009
125-129	21.652377956244365	27.953283991989906	28.260733922340876	22.13360412942485
130-134	21.687261765677952	28.088347538703996	28.17725964031519	22.047131055302863
135-139	21.904479607232897	27.962930277115234	28.038332217601663	22.094257898050206
140-144	21.779333158066212	28.065716861310104	28.21059590622695	21.94435407439674
145-149	21.652241501073252	28.199734451640683	28.263795124973484	21.88422892231258
150-154	21.728336912992965	28.200831256483422	28.175786803585734	21.89504502693788
155-159	21.60185178100607	28.239522877714784	28.341620344691776	21.81700499658737
160-164	21.489745605647006	28.439840119483684	28.363226174079514	21.707188100789796
165-169	21.5434116586955	28.44417827684203	28.261066056699246	21.751344007763223
170-174	21.444704824489467	28.59998936511034	28.351754563799286	21.60355124660091
175-179	21.383924788944913	28.77643956345832	28.334353561252378	21.505282086344383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	2.0
8	5.0
9	18.5
10	33.0
11	59.0
12	136.5
13	257.0
14	478.0
15	846.5
16	1431.5
17	2440.5
18	3959.5
19	6213.5
20	9442.0
21	14047.5
22	20087.0
23	27669.0
24	37390.0
25	49183.0
26	62879.5
27	78593.5
28	96034.0
29	115295.5
30	136194.0
31	158706.0
32	182629.5
33	207236.0
34	233370.5
35	259806.5
36	285920.5
37	311381.0
38	334686.5
39	354697.5
40	371114.5
41	383716.5
42	390514.0
43	390796.5
44	385068.5
45	373247.5
46	356605.0
47	336043.5
48	312792.5
49	287887.0
50	262681.0
51	239306.5
52	217073.0
53	196687.5
54	179122.5
55	163745.0
56	151045.5
57	139891.5
58	129156.0
59	119970.5
60	111605.5
61	102756.5
62	93463.0
63	84213.0
64	74787.0
65	65121.5
66	55596.5
67	46600.0
68	37935.0
69	29903.5
70	23108.5
71	17500.0
72	12836.5
73	9055.5
74	6264.5
75	4190.5
76	2694.0
77	1722.5
78	1068.5
79	617.0
80	341.5
81	192.0
82	101.0
83	59.0
84	33.5
85	14.5
86	6.5
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1743.0
92-93	3631.0
94-95	5230.0
96-97	6742.0
98-99	8096.0
100-101	9532.0
102-103	10847.0
104-105	12253.0
106-107	13913.0
108-109	14652.0
110-111	15770.0
112-113	16624.0
114-115	17662.0
116-117	18691.0
118-119	19343.0
120-121	20361.0
122-123	21434.0
124-125	22402.0
126-127	23172.0
128-129	24223.0
130-131	25085.0
132-133	26995.0
134-135	106758.0
136-137	106155.0
138-139	91622.0
140-141	89949.0
142-143	90344.0
144-145	95119.0
146-147	94026.0
148-149	97660.0
150-151	98329.0
152-153	95740.0
154-155	95340.0
156-157	96696.0
158-159	95064.0
160-161	101551.0
162-163	99129.0
164-165	99754.0
166-167	104775.0
168-169	110288.0
170-171	106660.0
172-173	113054.0
174-175	118902.0
176-177	128604.0
178-179	6584380.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.90887541441974
#Duplication Level	Percentage of total
1	85.32939226667335
2	11.132567090900361
3	2.250851585233775
4	0.6325540326470245
5	0.29095301120989425
6	0.1375456412347281
7	0.06914750242554356
8	0.031129936586053102
9	0.021909233622559244
>10	0.09780928760064339
>50	0.002667971461079137
>100	0.0034724404049468146
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.1838114060469738E-5	1.0919057030234869E-5	0.0	1.0919057030234869E-5	0.0	0.0
2	8.735245624187895E-5	1.0919057030234869E-5	0.0	1.0919057030234869E-5	0.0	0.0
3	9.827151327211383E-5	1.0919057030234869E-5	1.0919057030234869E-5	1.0919057030234869E-5	0.0	0.0
4	1.0919057030234868E-4	1.0919057030234869E-5	3.2757171090704604E-5	2.1838114060469738E-5	0.0	0.0
5	1.2010962733258356E-4	1.0919057030234869E-5	4.3676228120939476E-5	2.1838114060469738E-5	0.0	0.0
6	1.3102868436281842E-4	1.0919057030234869E-5	4.3676228120939476E-5	2.1838114060469738E-5	0.0	0.0
7	1.3102868436281842E-4	1.0919057030234869E-5	4.3676228120939476E-5	3.2757171090704604E-5	0.0	0.0
8	1.419477413930533E-4	1.0919057030234869E-5	5.459528515117434E-5	6.551434218140921E-5	0.0	0.0
9	1.419477413930533E-4	2.1838114060469738E-5	5.459528515117434E-5	7.643339921164408E-5	0.0	0.0
10-14	1.7907253529585184E-4	3.0573359684657635E-5	6.988196499350316E-5	9.390389046001987E-5	6.551434218140921E-6	0.0
15-19	2.0527827216841555E-4	3.9308605308845525E-5	1.004553246781608E-4	1.3539630717491238E-4	2.1838114060469738E-5	0.0
20-24	2.555059345074959E-4	8.735245624187895E-5	1.2010962733258356E-4	1.7252110107771095E-4	2.838954827861066E-5	0.0
25-29	2.729764257558717E-4	1.2010962733258356E-4	1.2884487295677146E-4	2.3148400904097918E-4	3.2757171090704604E-5	1.0919057030234869E-5
30-34	7.272091982136423E-4	1.5505060982933514E-4	1.5723442123538213E-4	2.5987355731958993E-4	3.2757171090704604E-5	1.0919057030234869E-5
35-39	0.002098642761211142	2.0964589498050952E-4	1.8780778092003975E-4	3.1228503106471724E-4	4.804385093303342E-5	2.6205736872563683E-5
40-44	0.0031097474422108907	2.4895450028935504E-4	2.4677068888330803E-4	3.6251269340379765E-4	8.735245624187895E-5	4.586003952698645E-5
45-49	0.004278086544446022	3.079174082526233E-4	2.838954827861066E-4	3.930860530884553E-4	1.4849917561119422E-4	6.551434218140921E-5
50-54	0.005638601050413286	3.36306956531234E-4	3.057335968465763E-4	4.4768133823962957E-4	1.7907253529585184E-4	8.080102202373803E-5
55-59	0.006767631547339571	3.712479390279856E-4	3.1446884247076425E-4	5.131956804210389E-4	2.48954500289355E-4	8.953626764792592E-5
60-64	0.007695751394909536	4.061889215247371E-4	3.3630695653123397E-4	5.175633032331328E-4	3.057335968465763E-4	9.827151327211383E-5
65-69	0.00843606346155946	4.6296801808195836E-4	3.7343175043403254E-4	5.328499830754617E-4	3.537774477796097E-4	1.0700675889630171E-4
70-74	0.014952556697203629	4.957251891726631E-4	3.930860530884553E-4	5.415852286996495E-4	3.821669960582204E-4	1.0919057030234868E-4
75-79	0.13666073397901357	5.415852286996495E-4	3.952698644945023E-4	5.89629079632683E-4	4.105565443368311E-4	1.1137438170839567E-4
80-84	0.18841706430232685	5.568719085419783E-4	4.12740355742878E-4	6.420405533778103E-4	5.306661716694146E-4	1.2010962733258356E-4
85-89	0.18876647412729436	5.699747769782602E-4	4.280270355852069E-4	6.66062478844327E-4	8.080102202373803E-4	1.2010962733258356E-4
90-94	0.18915300874616467	6.595110446261861E-4	4.520489610517236E-4	7.206577639955015E-4	8.25480711485756E-4	1.2010962733258356E-4
95-99	0.18956138147909546	7.075548955592194E-4	4.760708865182403E-4	7.337606324317831E-4	8.2984833429785E-4	1.2010962733258356E-4
100-104	0.18997630564624438	7.774368605527227E-4	4.9790900057871E-4	7.424958780559711E-4	8.2984833429785E-4	1.2010962733258356E-4
105-109	0.19050042038369566	8.189292772676152E-4	5.262985488573206E-4	7.555987464922529E-4	8.2984833429785E-4	1.244772501446775E-4
110-114	0.19103982180098927	8.626055053885546E-4	5.634233427601193E-4	7.708854263345817E-4	8.2984833429785E-4	1.3102868436281842E-4
115-119	0.19149842219625915	9.237522247578699E-4	5.80893834008495E-4	8.080102202373803E-4	8.429512027341319E-4	1.3102868436281842E-4
120-124	0.19262745269318543	0.001008920869593702	5.896290796326829E-4	8.538702597643668E-4	8.647893167946016E-4	1.332124957688654E-4
125-129	0.19498815282312218	0.0010438618520904534	5.961805138508238E-4	9.084655449155411E-4	8.909950536671651E-4	1.4849917561119422E-4
130-134	0.19773975519474138	0.0010547809091206882	6.398567419717634E-4	9.281198475699639E-4	9.215684133518229E-4	1.5505060982933514E-4
135-139	0.2005262985488573	0.0011093761942718625	6.6387866743828E-4	9.390389046001986E-4	9.586932072546213E-4	1.6378585545352305E-4
140-144	0.2032123865782951	0.0011399495539565203	6.900844043108438E-4	9.477741502243866E-4	9.696122642848564E-4	1.747049124837579E-4
145-149	0.2059312317788236	0.0012010962733258357	7.097387069652665E-4	9.914503783453263E-4	9.805313213150913E-4	1.7688872388980486E-4
150-154	0.20829848334297849	0.0012535077470709629	7.337606324317831E-4	0.0010220237380299836	9.89266566939279E-4	1.8562396951399276E-4
155-159	0.21076837404321763	0.0013037354094100435	7.708854263345818E-4	0.0010373104178723125	0.0010263913608420778	1.8562396951399276E-4
160-164	0.21355273358592752	0.0013102868436281844	7.927235403950515E-4	0.0010504132863085943	0.0010329427950602189	1.8780778092003975E-4
165-168	0.21564864658288108	0.0013184761364008603	8.025506917222628E-4	0.0010755271174781346	0.0010454997106449887	1.9654302654422765E-4
>>END_MODULE
