##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479315
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9065405
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.162811700084	39.0	39.0	39.0	37.0	39.0
2	39.8506960251638	41.0	40.0	41.0	38.0	41.0
3	39.89797697951719	41.0	40.0	41.0	38.0	41.0
4	39.911090127799035	41.0	40.0	41.0	38.0	41.0
5	39.91578920081342	41.0	40.0	41.0	38.0	41.0
6	39.90370865945868	41.0	40.0	41.0	38.0	41.0
7	39.92643119639994	41.0	40.0	41.0	38.0	41.0
8	39.91107049271378	41.0	40.0	41.0	38.0	41.0
9	39.901103701379036	41.0	40.0	41.0	38.0	41.0
10-14	39.834839259801406	41.0	40.0	41.0	38.0	41.0
15-19	39.71533062229432	41.0	39.6	41.0	37.2	41.0
20-24	39.55461241941204	40.0	39.0	41.0	37.0	41.0
25-29	39.35589216367057	40.0	38.8	41.0	36.0	41.0
30-34	39.268787505908456	40.0	38.6	41.0	35.4	41.0
35-39	38.914288992052754	40.0	38.0	41.0	35.0	41.0
40-44	38.665390547912644	40.0	37.2	41.0	35.0	41.0
45-49	38.112945775726516	39.2	35.6	41.0	34.4	41.0
50-54	37.46474583319774	37.8	35.0	40.2	34.0	41.0
55-59	36.77325899946003	36.4	35.0	39.2	33.8	41.0
60-64	36.00050954149318	35.4	34.8	38.0	32.8	40.0
65-69	35.5520730072181	35.0	34.8	36.8	33.0	39.0
70-74	35.24977615451267	35.0	35.0	36.0	33.0	38.2
75-79	35.027966759345006	35.0	35.0	35.6	33.0	37.0
80-84	34.93754264701908	35.0	35.0	35.0	33.0	37.0
85-89	34.76599677565426	35.0	34.0	35.0	32.6	37.2
90-94	39.053042703439566	40.2	38.6	40.2	36.4	40.2
95-99	39.576952163605725	41.0	39.0	41.0	36.6	41.0
100-104	39.481654087585085	41.0	39.0	41.0	36.0	41.0
105-109	39.34211866862763	40.0	39.0	41.0	35.8	41.0
110-114	39.168333893212235	40.0	38.2	41.0	35.0	41.0
115-119	38.9330390151143	40.0	38.0	41.0	35.0	41.0
120-124	38.64460418948431	40.0	37.8	41.0	34.4	41.0
125-129	38.330034095794005	39.8	36.8	40.8	33.8	40.8
130-134	37.97946260366133	39.0	35.8	40.6	34.0	41.0
135-139	37.69527655815883	39.0	35.0	40.8	33.6	41.0
140-144	37.401323674679055	37.8	35.0	40.4	34.0	41.0
145-149	36.75365633577259	36.4	35.0	39.4	34.0	41.0
150-154	36.07294480592027	35.4	35.0	38.0	33.0	40.2
155-159	35.470200377989485	35.0	35.0	36.6	33.0	38.8
160-164	35.00412798757678	35.0	35.0	35.8	33.0	37.0
165-169	34.70044900820361	35.0	35.0	35.0	33.0	36.0
170-174	34.530797892424324	35.0	34.2	35.0	33.0	36.0
175-179	34.26655820406515	35.0	33.8	35.0	32.4	35.8
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	8.0
29	125.0
30	1169.0
31	7089.0
32	32758.0
33	111852.0
34	300544.0
35	675024.0
36	1445644.0
37	2607334.0
38	2957473.0
39	914804.0
40	11581.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.552131427112194	28.031753683371015	28.193467363013568	22.222647526503227
2	21.44079608136647	28.456731938617192	29.60435854768761	20.498113432328726
3	20.784531965201776	27.141379783914783	29.6533359513447	22.42075229953874
4	21.178789033694578	27.04815725276477	29.762663664778355	22.0103900487623
5	21.589074067843633	27.686849070725465	29.105097896894844	21.618978964536055
6	22.411497335199034	27.124954704174826	28.22339432159953	22.24015363902661
7	22.069626232915134	27.91263048920594	28.14764481013259	21.870098467746338
8	21.750732592752335	28.074983963761134	28.672607566898556	21.501675876587974
9	22.583491857230868	27.517325480770026	27.637783419494223	22.261399242504883
10-14	22.067656105822078	27.946100587894307	28.068105065355603	21.918138240928013
15-19	22.134280818121198	27.84290608086456	27.99466322795286	22.02814987306138
20-24	21.961099366216956	27.90333360726851	28.155602535132186	21.979964491382347
25-29	21.87039189093041	28.151022486033444	28.0292452460756	21.949340376960542
30-34	21.991551397869152	28.142162429588087	27.87042829305475	21.99585787948801
35-39	21.873427607481407	28.205672002519467	27.958077989896758	21.962822400102368
40-44	21.878949699434276	28.341134235039693	27.92515943854687	21.854756626979157
45-49	22.132714423679914	28.15188510607083	27.713235095398385	22.002165374850875
50-54	22.03439118274363	28.205627878732393	27.862695599369253	21.89728533915473
55-59	21.944387481860986	28.273419665199732	27.905861900268107	21.87633095267117
60-64	22.035227328508764	28.19780031890467	27.792803520636973	21.97416883194959
65-69	21.96171048066799	28.176195106561703	27.918765901799205	21.943328510971106
70-74	21.88680152734489	28.33334418043099	27.881126105231925	21.898728186992198
75-79	22.019490579847233	28.227447091442688	27.730251433885194	22.022810894824886
80-84	21.958763011691147	28.27947124259754	27.78644307672961	21.975322668981693
85-89	21.9719802921105	28.356818035156728	27.693330855047293	21.977870817685478
90-94	21.200326320606287	27.342180981179187	29.583481334546057	21.874011363668462
95-99	21.77726006710197	27.749196949429944	28.468614811772763	22.00492817169533
100-104	21.776633840723928	27.975707041666343	28.243615017948564	22.004044099661165
105-109	21.851660891242602	27.860929392397495	28.192657838881086	22.094751877478817
110-114	21.7297203840641	27.83095773431954	28.357788912493632	22.081532969122733
115-119	21.650794232254945	27.983157870920333	28.277632959176778	22.088414937647944
120-124	21.74524830020518	27.96066597667135	28.162913769981284	22.131171953142186
125-129	21.653324596613828	27.97334727680304	28.280514621695296	22.092813504887843
130-134	21.671541123947033	28.111207626500377	28.205284552752413	22.011966696800176
135-139	21.887439681003933	27.995529174148153	28.045184435048835	22.071846709799082
140-144	21.76316848652514	28.073079183709265	28.24260810647826	21.92114422328733
145-149	21.646166285583814	28.210344876305122	28.277972225094018	21.86551661301705
150-154	21.71414528850931	28.21211703638444	28.20203986696902	21.871697808137224
155-159	21.583905940854347	28.260243652097678	28.362438162749353	21.793412244298622
160-164	21.489850833087765	28.46311361606589	28.363291454630048	21.683744096216298
165-169	21.54059520330424	28.458012344740187	28.26475422199958	21.736638229955997
170-174	21.44275730299472	28.6158002036118	28.35551575461575	21.58592673877773
175-179	21.382639950249803	28.790176636323388	28.34503253022659	21.482150883200216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	2.0
7	3.0
8	8.5
9	20.0
10	40.5
11	73.0
12	149.5
13	275.5
14	480.0
15	849.0
16	1464.0
17	2462.5
18	4069.0
19	6299.0
20	9553.5
21	14179.0
22	20063.5
23	27777.0
24	37739.5
25	49436.5
26	62906.0
27	78307.0
28	95623.5
29	115001.0
30	135734.5
31	158100.5
32	182010.0
33	206935.5
34	232656.5
35	257499.0
36	283486.0
37	309083.0
38	331175.0
39	351796.5
40	368955.5
41	380507.5
42	386435.0
43	386482.5
44	380842.0
45	369596.0
46	352400.5
47	331648.0
48	309336.0
49	284899.5
50	259879.5
51	236235.5
52	214554.0
53	194765.0
54	176926.0
55	161376.0
56	148336.5
57	137435.0
58	127455.5
59	118132.5
60	109483.5
61	100505.0
62	91976.5
63	83154.5
64	73475.5
65	63830.5
66	54416.5
67	45342.0
68	36759.0
69	29238.5
70	22593.5
71	16948.0
72	12289.5
73	8688.0
74	6097.5
75	4027.0
76	2525.0
77	1606.0
78	980.5
79	569.0
80	317.5
81	175.5
82	103.5
83	55.5
84	20.5
85	8.0
86	5.5
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1871.0
92-93	4040.0
94-95	5654.0
96-97	7378.0
98-99	9002.0
100-101	10456.0
102-103	11644.0
104-105	12918.0
106-107	14397.0
108-109	15575.0
110-111	16576.0
112-113	17326.0
114-115	18432.0
116-117	19518.0
118-119	20461.0
120-121	22954.0
122-123	22327.0
124-125	24522.0
126-127	25175.0
128-129	25868.0
130-131	26879.0
132-133	30593.0
134-135	118649.0
136-137	113044.0
138-139	99262.0
140-141	99428.0
142-143	92449.0
144-145	101654.0
146-147	99871.0
148-149	103148.0
150-151	97334.0
152-153	93494.0
154-155	93187.0
156-157	93982.0
158-159	101161.0
160-161	103923.0
162-163	99102.0
164-165	104314.0
166-167	100480.0
168-169	104252.0
170-171	106998.0
172-173	112888.0
174-175	115118.0
176-177	132842.0
178-179	6415259.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.99737450933233
#Duplication Level	Percentage of total
1	85.49163963639838
2	10.99264194870477
3	2.2400070396883054
4	0.6671911368854365
5	0.24609032036551462
6	0.13039752046055164
7	0.07226821182923052
8	0.04048489955475748
9	0.022962853982415637
>10	0.09126526860730765
>50	0.0014360451792483294
>100	0.0036151183441153196
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.1030946769614816E-5	0.0	0.0
2	2.206189353922963E-5	0.0	0.0	3.309284030884445E-5	0.0	0.0
3	3.309284030884445E-5	0.0	0.0	3.309284030884445E-5	0.0	0.0
4	3.309284030884445E-5	2.206189353922963E-5	0.0	3.309284030884445E-5	1.1030946769614816E-5	0.0
5	3.309284030884445E-5	3.309284030884445E-5	0.0	6.61856806176889E-5	2.206189353922963E-5	0.0
6	3.309284030884445E-5	4.412378707845926E-5	1.1030946769614816E-5	6.61856806176889E-5	3.309284030884445E-5	0.0
7	3.309284030884445E-5	6.61856806176889E-5	2.206189353922963E-5	7.721662738730372E-5	3.309284030884445E-5	0.0
8	3.309284030884445E-5	7.721662738730372E-5	3.309284030884445E-5	8.824757415691853E-5	3.309284030884445E-5	0.0
9	4.412378707845926E-5	8.824757415691853E-5	3.309284030884445E-5	8.824757415691853E-5	3.309284030884445E-5	0.0
10-14	7.28042486794578E-5	1.081032783422252E-4	3.529902966276741E-5	1.1030946769614817E-4	3.309284030884445E-5	0.0
15-19	1.0148471028045632E-4	1.5002087606676148E-4	5.294854449415112E-5	1.4340230800499263E-4	3.309284030884445E-5	0.0
20-24	1.3016517188145483E-4	2.0076323120698966E-4	6.397949126376595E-5	1.5443325477460743E-4	3.309284030884445E-5	0.0
25-29	1.6546420154422224E-4	2.6474272247075563E-4	1.1472184640399408E-4	1.8531990572952895E-4	3.309284030884445E-5	0.0
30-34	4.985987939865896E-4	3.2651602438059856E-4	1.676703908981452E-4	2.2282512474621928E-4	3.750521901669037E-5	0.0
35-39	0.0015840439561166874	3.838769475825956E-4	2.4268082893152596E-4	2.537117757011408E-4	8.38351954490726E-5	0.0
40-44	0.0030202732255205367	4.2358835595320894E-4	2.845984266560622E-4	2.934231840717541E-4	1.389899292971467E-4	4.412378707845926E-6
45-49	0.0043329558911047	4.544750069081304E-4	3.154850776109838E-4	3.221036456727526E-4	1.7428895895991412E-4	3.529902966276741E-5
50-54	0.005603720958964327	4.919802259248208E-4	3.6181505404336595E-4	3.375469711502134E-4	2.0296942056091265E-4	5.074235514022815E-5
55-59	0.006742114665588575	5.31691634295434E-4	3.949078943522104E-4	3.5299029662767405E-4	2.162065566844504E-4	6.618568061768891E-5
60-64	0.007779023661932369	5.802278000817393E-4	4.257945453071319E-4	3.7946456887474965E-4	2.2503131410014227E-4	1.1251565705007113E-4
65-69	0.008458529982940641	6.243515871601987E-4	4.544750069081304E-4	3.8608313693651856E-4	2.448870182854489E-4	1.2134041446576298E-4
70-74	0.014382148398223799	6.662691848847348E-4	4.941864152787438E-4	4.1035121982967116E-4	2.735674798864474E-4	1.2134041446576298E-4
75-79	0.12686250641863211	7.059805932553482E-4	5.40516391711126E-4	4.4344406013851556E-4	3.154850776109837E-4	1.2134041446576298E-4
80-84	0.17532366176690398	7.611353271034222E-4	5.603720958964326E-4	4.7874308980128306E-4	4.544750069081304E-4	1.2134041446576298E-4
85-89	0.17569209538900907	8.140838715975735E-4	5.736092320199704E-4	5.008049833405126E-4	7.986405461201127E-4	1.2134041446576298E-4
90-94	0.17599213714114262	8.38351954490726E-4	5.956711255592001E-4	5.3610401300328E-4	8.118776822436504E-4	1.2134041446576298E-4
95-99	0.17632085935487715	8.758571735074164E-4	6.04495882974892E-4	5.846401787895853E-4	8.273210077211112E-4	1.323713612353778E-4
100-104	0.17663193205378028	9.310119073554904E-4	6.397949126376593E-4	6.331763445758904E-4	8.471767119064179E-4	1.323713612353778E-4
105-109	0.17701801519071678	9.994037773271025E-4	7.170115400249629E-4	6.59650616822966E-4	8.736509841534934E-4	1.323713612353778E-4
110-114	0.17741954165313079	0.0010192594815124092	7.390734335641927E-4	7.280424867945779E-4	8.824757415691854E-4	1.323713612353778E-4
115-119	0.1778740166600389	0.001025878049574178	7.765786525808831E-4	7.765786525808831E-4	8.824757415691854E-4	1.323713612353778E-4
120-124	0.17875208002290024	0.0010942699195457897	8.118776822436504E-4	8.251148183671883E-4	8.824757415691854E-4	1.3898992929714665E-4
125-129	0.18094503224069966	0.001129568949208557	8.427643331985719E-4	8.604138480299556E-4	9.001252564005688E-4	1.434023080049926E-4
130-134	0.18331006722810508	0.0011758989256409394	8.780633628613394E-4	8.890943096309542E-4	9.376304754172592E-4	1.456084973589156E-4
135-139	0.18606780392050876	0.0012178165233654756	8.868881202770313E-4	9.177747712319526E-4	9.508676115407972E-4	1.5443325477460743E-4
140-144	0.18884980869580564	0.0012884145826910106	9.089500138162608E-4	9.354242860633363E-4	9.729295050800267E-4	1.5443325477460743E-4
145-149	0.1910515856710208	0.0013303321804155467	9.310119073554904E-4	9.376304754172592E-4	0.0010236718602202547	1.6546420154422224E-4
150-154	0.19307907368727598	0.0013788683462018518	9.464552328329512E-4	9.464552328329512E-4	0.0010589708898830223	1.6546420154422224E-4
155-159	0.19551691292336085	0.0014362292694038489	0.0010060223453888712	0.0010082285347427942	0.001063383268590868	1.6546420154422224E-4
160-164	0.198261412479641	0.0014737344884205395	0.0010347028069898699	0.0010523523218212536	0.001070001836652637	1.7428895895991412E-4
165-168	0.2003357820196671	0.0014781468671283854	0.0010672440999602334	0.0010755173100374445	0.0011058524136538853	1.8752609508345186E-4
>>END_MODULE
