##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479335
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2374864
Total Bases	376.4 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.45625433709046	39.0	39.0	39.0	37.0	39.0
2	40.15638032325219	41.0	40.0	41.0	38.0	41.0
3	40.14899884793403	41.0	40.0	41.0	38.0	41.0
4	40.12378856220819	41.0	40.0	41.0	38.0	41.0
5	40.09544378120179	41.0	40.0	41.0	38.0	41.0
6	40.06260021626502	41.0	40.0	41.0	38.0	41.0
7	40.05278828598185	41.0	40.0	41.0	38.0	41.0
8	39.99671475924516	41.0	40.0	41.0	38.0	41.0
9	39.95528080765888	41.0	40.0	41.0	38.0	41.0
10-14	40.06474307581403	41.0	40.0	41.0	38.0	41.0
15-19	39.93903120347102	41.0	40.0	41.0	38.0	41.0
20-24	39.718428592121484	40.2	39.4	41.0	37.8	41.0
25-29	39.46044329275277	40.0	39.0	41.0	36.8	41.0
30-34	39.10771530496062	40.0	38.0	41.0	35.6	41.0
35-39	38.784043296795105	40.0	38.0	41.0	35.0	41.0
40-44	38.26915638116541	39.4	36.8	40.6	34.2	41.0
45-49	37.60007175147714	38.6	35.4	40.0	33.4	41.0
50-54	36.83646154053453	37.4	35.0	39.8	32.6	41.0
55-59	36.10575123459701	36.2	34.2	39.0	31.6	40.2
60-64	35.30728504874385	35.0	33.6	38.0	30.6	40.0
65-69	35.39628105019908	35.0	33.8	37.8	31.6	40.0
70-74	35.29461341786308	35.0	34.0	37.0	31.4	40.0
75-79	35.16751451872612	35.0	34.0	37.2	31.0	40.0
80-84	35.267464915885704	35.0	34.0	38.4	31.0	41.0
85-89	35.54484753653262	35.4	33.6	39.4	30.2	41.0
90-94	39.27015603765203	39.8	38.8	40.8	36.0	41.0
95-99	39.22341740747501	40.0	38.6	41.0	35.8	41.0
100-104	39.093579381043625	40.0	38.0	41.0	35.0	41.0
105-109	38.84831365001564	40.0	38.0	41.0	34.8	41.0
110-114	38.529873272969844	40.0	37.4	41.0	34.0	41.0
115-119	38.19261986357857	39.8	36.6	41.0	34.0	41.0
120-124	37.79662990481571	39.0	35.6	40.2	33.0	41.0
125-129	37.14550785283521	38.0	35.0	39.8	32.8	40.6
130-134	37.171946412140535	38.0	35.0	40.0	33.0	41.0
135-139	36.71515654375153	37.2	35.0	40.0	32.2	41.0
140-144	36.188557587096895	36.2	34.4	39.0	32.0	40.8
145-149	35.47872086091262	35.0	34.0	37.8	31.4	39.8
150-154	34.80755770603614	35.0	34.0	36.4	31.0	38.8
155-159	34.17384700514948	35.0	33.2	35.4	30.8	37.0
160-164	33.69250112200543	34.4	33.0	35.0	30.0	36.0
165-169	33.35100166564063	34.0	32.6	35.0	29.4	35.4
170-174	33.106503730862116	34.0	32.0	35.0	29.0	35.0
175-178	32.2537654534307	33.25	30.75	34.75	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	2.0
31	97.0
32	1045.0
33	7603.0
34	41072.0
35	175372.0
36	549176.0
37	980603.0
38	572520.0
39	47198.0
40	176.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.737355907538284	28.525001852737674	29.137331653517844	21.600310586206202
2	21.47861940725869	28.787374771776403	29.254138342237702	20.479867478727204
3	20.339817353751624	28.222247673972067	29.798548464248903	21.639386508027407
4	20.608295885574922	27.735356635158897	30.375002526460467	21.28134495280572
5	21.107861334375357	28.353076218259236	29.162385719771745	21.37667672759366
6	21.21927824077505	28.399731521468176	28.9668376799682	21.414152557788572
7	21.1156091464606	28.694358919079153	28.961532112996785	21.228499821463462
8	21.0825546220752	28.89175969655526	28.954626454399072	21.07105922697047
9	21.3122940934723	28.733855917644124	28.731203134158417	21.222646854725156
10-14	21.16626467873529	28.700725599444855	28.846990817158375	21.28601890466149
15-19	21.330290913500733	28.51839936939547	28.739380444522293	21.411929272581503
20-24	21.271188581746156	28.50411644624703	28.76172277654636	21.462972195460456
25-29	21.282776613734512	28.50530388266444	28.653640797957276	21.558278705643776
30-34	21.315570070538776	28.534433971797963	28.592778365413768	21.557217592249494
35-39	21.314955298492883	28.5064407898726	28.620527322827748	21.558076588806767
40-44	21.33534383442589	28.517498265163816	28.584104184492247	21.56305371591805
45-49	21.55713337690074	28.416776708055703	28.429594284135852	21.596495630907704
50-54	21.473937033868047	28.440163310404298	28.49417903509422	21.591720620633435
55-59	21.362031678445586	28.43236496910981	28.586260097420315	21.619343255024287
60-64	21.266068288541998	28.438933766312513	28.64390550364147	21.65109244150402
65-69	21.179739134535705	28.430402751483875	28.761419601290854	21.62843851268957
70-74	21.10730551307359	28.437678957616097	28.837988196376717	21.617027332933592
75-79	21.038097339468703	28.358727068160533	28.951847347890237	21.651328244480524
80-84	20.916540905079195	28.367072809221916	29.078052469530885	21.638333816168
85-89	20.85652062602322	28.190237419911202	29.37321042383901	21.580031530226574
90-94	20.902967148655332	28.49716993975928	29.222579284147756	21.37728362743763
95-99	21.07420298574673	28.72870178620005	28.839244022514503	21.357851205538715
100-104	21.172549937566103	28.61495745860289	28.76921040541746	21.44328219841354
105-109	21.164686769917356	28.613457198524912	28.747763278637745	21.474092752919987
110-114	21.236085055111474	28.53385290326879	28.705588257136448	21.524473784483277
115-119	21.224456254442785	28.599657573924127	28.665010907799427	21.510875263833665
120-124	21.15083974190538	28.674791827741757	28.68227228336362	21.492096146989244
125-129	21.168103590084435	28.719649667436915	28.68596455494435	21.42628218753431
130-134	21.21288744233036	28.728716006365325	28.689068801555333	21.369327749748983
135-139	21.196496582516307	28.815313794801668	28.71078643666969	21.27740318601234
140-144	21.135946406604823	28.896480992702823	28.81968318612154	21.147889414570813
145-149	21.016830665457352	28.978543952691044	28.928637914346627	21.075987467504977
150-154	20.923584285817	29.11714137226809	29.02433314533686	20.93494119657805
155-159	20.79340045768589	29.226593275336494	29.159610857811668	20.820395409165947
160-164	20.619644230332895	29.451244789105168	29.296726963214976	20.63238401734696
165-169	20.339280577029843	29.58254767226759	29.63067396995883	20.44749778074373
170-174	20.15919765121242	29.762678429040896	29.917719128321856	20.160404791424828
175-178	20.014686508694858	29.700278762284515	30.456802006869598	19.82823272215103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	3.0
11	5.5
12	15.5
13	41.0
14	91.5
15	182.5
16	307.0
17	590.0
18	1068.5
19	1823.0
20	2912.0
21	4358.5
22	6326.5
23	8826.0
24	11891.5
25	15646.5
26	19869.0
27	24484.0
28	29610.5
29	35041.0
30	40789.5
31	46905.5
32	53346.0
33	59733.5
34	66010.0
35	72908.0
36	79571.0
37	85869.0
38	91883.0
39	97541.0
40	102397.5
41	105259.0
42	106847.0
43	106859.0
44	104949.5
45	101118.0
46	95567.0
47	88773.5
48	81426.5
49	73822.5
50	65633.0
51	57778.5
52	50858.0
53	44477.5
54	39135.5
55	34880.0
56	31153.0
57	28068.5
58	25498.0
59	23407.0
60	21535.0
61	19764.0
62	17834.5
63	15876.0
64	14157.5
65	12470.0
66	10655.5
67	8807.5
68	7151.5
69	5690.5
70	4464.5
71	3420.5
72	2533.5
73	1798.5
74	1287.0
75	898.0
76	556.0
77	355.0
78	242.5
79	149.5
80	82.0
81	53.0
82	32.0
83	13.0
84	6.0
85	5.0
86	3.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	708.0
92-93	1576.0
94-95	2509.0
96-97	3578.0
98-99	4636.0
100-101	5881.0
102-103	7284.0
104-105	8718.0
106-107	10235.0
108-109	11565.0
110-111	12545.0
112-113	13779.0
114-115	15170.0
116-117	16317.0
118-119	18348.0
120-121	19811.0
122-123	21570.0
124-125	23439.0
126-127	25497.0
128-129	27239.0
130-131	28871.0
132-133	33330.0
134-135	45940.0
136-137	46332.0
138-139	46225.0
140-141	47908.0
142-143	49326.0
144-145	52511.0
146-147	53823.0
148-149	55596.0
150-151	58088.0
152-153	53898.0
154-155	56856.0
156-157	59644.0
158-159	63322.0
160-161	66936.0
162-163	71707.0
164-165	74405.0
166-167	78478.0
168-169	82476.0
170-171	86648.0
172-173	90446.0
174-175	101066.0
176-177	295793.0
178-179	424834.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.70436491450366
#Duplication Level	Percentage of total
1	97.45063659632783
2	2.438023146500513
3	0.09176334870927824
4	0.009583962797930091
5	0.005436528062053231
6	0.0022200547908916954
7	0.0
8	0.0011563711806735681
9	0.001179991630831977
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	4.210767437630113E-5	0.0	0.0	0.0
2	0.0	0.0	4.210767437630113E-5	0.0	0.0	0.0
3	0.0	4.210767437630113E-5	4.210767437630113E-5	0.0	0.0	0.0
4	4.210767437630113E-5	4.210767437630113E-5	4.210767437630113E-5	0.0	0.0	0.0
5	8.421534875260226E-5	4.210767437630113E-5	4.210767437630113E-5	0.0	0.0	0.0
6	1.2632302312890338E-4	8.421534875260226E-5	8.421534875260226E-5	0.0	4.210767437630113E-5	0.0
7	1.684306975052045E-4	8.421534875260226E-5	8.421534875260226E-5	0.0	4.210767437630113E-5	0.0
8	1.684306975052045E-4	8.421534875260226E-5	1.684306975052045E-4	0.0	4.210767437630113E-5	0.0
9	1.684306975052045E-4	8.421534875260226E-5	1.684306975052045E-4	0.0	1.2632302312890338E-4	0.0
10-14	1.684306975052045E-4	8.421534875260226E-5	2.0211683700624541E-4	8.421534875260226E-5	1.2632302312890338E-4	0.0
15-19	2.1053837188150567E-4	1.1790148825364315E-4	2.358029765072863E-4	8.421534875260226E-5	1.2632302312890338E-4	0.0
20-24	2.5264604625780676E-4	1.2632302312890338E-4	3.36861395010409E-4	1.347445580041636E-4	1.2632302312890338E-4	0.0
25-29	2.5264604625780676E-4	1.5158762775468407E-4	4.2107674376301135E-4	1.684306975052045E-4	1.4316609287942384E-4	0.0
30-34	3.621259996361897E-4	2.0211683700624541E-4	5.558213017671749E-4	1.684306975052045E-4	1.684306975052045E-4	8.421534875260226E-6
35-39	5.22135162266134E-4	2.9475372063410786E-4	6.905658597713384E-4	1.684306975052045E-4	1.684306975052045E-4	4.210767437630113E-5
40-44	8.16888882900242E-4	3.36861395010409E-4	7.579381387734203E-4	1.7685223238046474E-4	1.684306975052045E-4	4.210767437630113E-5
45-49	0.0010526918594075281	3.36861395010409E-4	8.842611619023237E-4	2.1053837188150567E-4	2.1895990675676585E-4	6.73722790020818E-5
50-54	0.00112006413840961	3.4528292988566926E-4	9.179473014033646E-4	2.1053837188150567E-4	2.5264604625780676E-4	8.421534875260226E-5
55-59	0.0011790148825364315	4.12655208887751E-4	9.684765106549259E-4	2.5264604625780676E-4	2.5264604625780676E-4	8.421534875260226E-5
60-64	0.001305337905665335	4.631844181393124E-4	9.684765106549259E-4	3.705475345114499E-4	2.7791065088358745E-4	8.421534875260226E-5
65-69	0.001347445580041636	4.631844181393124E-4	9.684765106549259E-4	4.4634134838879194E-4	3.9581213913723065E-4	8.421534875260226E-5
70-74	0.0038065337636176214	5.221351622661339E-4	0.0010358487896570078	4.884490227650932E-4	4.968705576403533E-4	8.421534875260226E-5
75-79	0.04770799506834918	5.473997668919146E-4	0.0010526918594075281	5.473997668919146E-4	6.316151156445169E-4	8.421534875260226E-5
80-84	0.0650226707718842	5.895074412682157E-4	0.0010611133942827883	5.810859063929555E-4	9.01104231652844E-4	8.421534875260226E-5
85-89	0.06516583686476363	5.895074412682157E-4	0.0010947995337838292	6.147720458939964E-4	0.0010947995337838292	8.421534875260226E-5
90-94	0.06537637523664512	6.147720458939964E-4	0.0011705933476611713	6.989873946465987E-4	0.0010947995337838292	8.421534875260226E-5
95-99	0.06551111979464928	6.316151156445169E-4	0.0012463871615385133	7.158304643971192E-4	0.0011116426035343496	8.421534875260226E-5
100-104	0.06553638439927507	6.484581853950374E-4	0.0013727101846674166	7.242519992723794E-4	0.0011369072081601303	8.421534875260226E-5
105-109	0.06558691360852663	7.916242782744611E-4	0.0013895532544179372	7.663596736486804E-4	0.0011369072081601303	8.421534875260226E-5
110-114	0.06573007970140606	8.000458131497215E-4	0.0013895532544179372	8.000458131497215E-4	0.0011369072081601303	8.421534875260226E-5
115-119	0.06594061807328756	8.000458131497215E-4	0.0013895532544179372	8.000458131497215E-4	0.0011705933476611713	8.421534875260226E-5
120-124	0.06626063639854746	8.253104177755021E-4	0.00149903320779632	8.000458131497215E-4	0.0012127010220374724	8.421534875260226E-5
125-129	0.06706068221169717	8.674180921518033E-4	0.0015579839519231416	8.337319526507623E-4	0.0012884948359148146	8.421534875260226E-5
130-134	0.0680291587223521	9.179473014033646E-4	0.0016085131611747027	8.758396270270635E-4	0.001347445580041636	8.421534875260226E-5
135-139	0.0694692411860216	9.432119060291452E-4	0.0016421993006757439	8.842611619023237E-4	0.001347445580041636	8.421534875260226E-5
140-144	0.07115354816107364	0.001010584185031227	0.0016590423704262644	8.842611619023237E-4	0.001381131719542677	8.421534875260226E-5
145-149	0.0744042606229241	0.001010584185031227	0.001717993114553086	8.842611619023237E-4	0.001456925533420019	8.421534875260226E-5
150-154	0.07740232703851672	0.001010584185031227	0.001751679254054127	8.842611619023237E-4	0.0014906116729210598	8.421534875260226E-5
155-159	0.07873292954880785	0.001010584185031227	0.0017685223238046474	8.842611619023237E-4	0.0015495624170478815	8.421534875260226E-5
160-164	0.08011406126835052	0.001010584185031227	0.0017685223238046474	8.842611619023237E-4	0.0015579839519231416	8.421534875260226E-5
165-167	0.0807484835622868	0.001010584185031227	0.0017685223238046472	8.842611619023236E-4	0.0015860557348406756	8.421534875260226E-5
>>END_MODULE
