##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479336
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11403209
Total Bases	1.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.32384463005107	39.0	39.0	39.0	37.0	39.0
2	40.0357673879344	41.0	40.0	41.0	38.0	41.0
3	40.06422770993674	41.0	40.0	41.0	38.0	41.0
4	40.07118540053067	41.0	40.0	41.0	38.0	41.0
5	40.06140289106338	41.0	40.0	41.0	38.0	41.0
6	40.05055515513221	41.0	40.0	41.0	38.0	41.0
7	40.07197149504144	41.0	40.0	41.0	38.0	41.0
8	40.05079026438961	41.0	40.0	41.0	38.0	41.0
9	40.02902367219613	41.0	40.0	41.0	38.0	41.0
10-14	39.9431839756686	41.0	40.0	41.0	38.0	41.0
15-19	39.749383458638704	40.2	39.8	41.0	37.8	41.0
20-24	39.567232311536166	40.0	39.0	41.0	37.0	41.0
25-29	39.48750666588677	40.0	39.0	41.0	36.8	41.0
30-34	39.51087614021632	40.0	39.0	41.0	36.6	41.0
35-39	39.276018531274836	40.0	38.8	41.0	35.4	41.0
40-44	38.83728618847554	40.0	37.6	41.0	35.0	41.0
45-49	38.24584646304387	39.4	36.4	41.0	34.2	41.0
50-54	37.57912154376895	38.4	35.2	40.0	34.0	41.0
55-59	36.857059780277645	36.8	35.0	39.6	33.0	41.0
60-64	35.9681771157575	35.8	34.6	38.4	32.6	40.0
65-69	35.57209566184396	35.0	34.2	37.0	32.8	39.2
70-74	35.299827794088486	35.0	34.0	36.4	33.0	39.0
75-79	35.08071198203944	35.0	34.0	36.0	32.6	39.0
80-84	35.01605693625364	35.0	34.0	36.0	32.0	39.6
85-89	34.894574746459526	34.8	33.8	36.0	31.4	40.0
90-94	39.39936099641452	40.6	38.8	40.6	36.8	40.8
95-99	39.59811911023638	40.8	39.0	41.0	36.8	41.0
100-104	39.41876470502949	40.0	39.0	41.0	36.0	41.0
105-109	39.18377534431311	40.0	38.2	41.0	35.4	41.0
110-114	38.90811089423359	40.0	38.0	41.0	35.0	41.0
115-119	38.699408302196076	40.0	38.0	41.0	34.8	41.0
120-124	38.79445998626102	40.0	38.0	41.0	35.0	41.0
125-129	38.20214083107872	39.4	36.6	40.6	33.8	40.8
130-134	38.17679801637445	39.4	36.0	40.8	34.0	41.0
135-139	37.75629040837074	38.8	35.0	40.8	34.0	41.0
140-144	37.13077878869809	37.4	35.0	40.0	33.2	41.0
145-149	36.411733999459514	36.2	35.0	39.0	33.0	40.8
150-154	35.68768427219363	35.2	34.8	37.2	33.0	39.4
155-159	35.05402274026183	35.0	34.0	36.2	32.2	38.0
160-164	34.56427693341807	35.0	34.0	35.4	32.0	36.8
165-169	34.23595392119229	35.0	34.0	35.0	31.8	36.0
170-174	34.03129345450703	35.0	34.0	35.0	31.0	35.8
175-178	33.69039661455987	34.75	33.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	88.0
31	1345.0
32	11616.0
33	61432.0
34	234880.0
35	728397.0
36	1878132.0
37	3733100.0
38	3909397.0
39	840187.0
40	4635.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.53429872240349	28.77864467800248	29.343634761057174	21.343421838536855
2	21.23412804237825	29.022584782932594	29.440037449107525	20.303249725581633
3	20.017838838172658	28.511158569486888	30.093037845750263	21.37796474659019
4	20.3080904682182	27.972853957162407	30.57686656449075	21.14218901012864
5	20.842036658277507	28.585462215065956	29.375231130114337	21.1972699965422
6	20.933958151604518	28.689906499126693	29.19208970036417	21.184045648904622
7	20.812018792254005	28.95897988013725	29.200297916139224	21.028703411469525
8	20.774415342207618	29.098949251916718	29.1872489577276	20.939386448148063
9	21.00615712647203	29.03050360648481	28.95682259265791	21.006516674385253
10-14	20.876169155542094	28.962642007175347	29.062684021664424	21.09850481561813
15-19	21.000751630527866	28.824859739043635	28.954153168638758	21.220235461789745
20-24	20.99217334348603	28.761895006923048	28.94170579527219	21.30422585431873
25-29	21.062206261412904	28.757710219991584	28.80675957092429	21.37332394767122
30-34	21.140498257990362	28.752188967158283	28.765160754310475	21.342152020540883
35-39	21.10418742653932	28.738808523109594	28.77785367259339	21.3791503777577
40-44	21.1374903327651	28.741439361499033	28.736303964962843	21.384766340773023
45-49	21.362104298886393	28.62423024957273	28.591539451745557	21.422125999795323
50-54	21.300058606309854	28.68296985523987	28.604642780817223	21.41232875763305
55-59	21.19849421333942	28.67982688031062	28.65238022033973	21.469298686010227
60-64	21.182277725506918	28.705074159387937	28.65168041732814	21.460967697777004
65-69	21.09047900463808	28.72277794785661	28.696462548393175	21.490280499112135
70-74	21.022524449038862	28.764092633924363	28.71220548531558	21.501177431721192
75-79	21.004768043802407	28.72158179333554	28.715729054865168	21.55792110799688
80-84	20.92837200475761	28.76524494113894	28.74040456506585	21.565978489037605
85-89	20.85328612323075	28.734171231975143	28.77102050834989	21.641522136444223
90-94	20.585513929831233	28.70095719128458	29.552475708841513	21.16105317004267
95-99	20.735945572776775	29.02891332060282	29.13248796497622	21.102653141644183
100-104	20.821863792023994	28.95917776108613	29.018237438041055	21.200721008848824
105-109	20.844794189118886	28.905195466137677	28.99522656402795	21.25478378071549
110-114	20.910434692314002	28.83520980481044	28.915574246438425	21.338781256437137
115-119	21.004306225186138	28.8541177514894	28.778412326049736	21.363163697274732
120-124	20.987828398658962	28.87481842983969	28.80207473031567	21.335278441185686
125-129	20.99916035042661	28.891729393271383	28.82205257509335	21.287057681208662
130-134	21.05750597673532	28.910835788131596	28.776960393036934	21.25469784209615
135-139	21.08177654334254	28.943006082513843	28.784321227548197	21.19089614659542
140-144	21.03594029369407	29.049065073360282	28.829847778007885	21.08514685493777
145-149	20.92312170772065	29.14620897594239	28.89737132449924	21.033297991837728
150-154	20.79896517823369	29.283830055973663	28.991173399291238	20.926031366501405
155-159	20.695938763250595	29.399266840696104	29.08403706338542	20.820757332667878
160-164	20.570270715132768	29.57361924895507	29.168109511372442	20.688000524539717
165-169	20.396464100928398	29.707051728494875	29.30346531493999	20.59301885563674
170-174	20.253724518189053	29.9030949129507	29.428438022786956	20.414742546073292
175-178	20.07344252679063	30.05281735074028	29.55069743784043	20.323042684628664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	2.5
9	4.5
10	14.0
11	53.0
12	120.0
13	246.0
14	521.0
15	1009.0
16	1928.5
17	3513.5
18	5964.5
19	9876.0
20	15576.0
21	23398.0
22	33672.5
23	46515.5
24	62432.0
25	81096.5
26	102293.5
27	125615.0
28	151187.5
29	177756.5
30	205834.5
31	235218.5
32	264910.5
33	295135.0
34	325268.0
35	356347.0
36	387458.5
37	417644.5
38	445346.0
39	470538.5
40	491453.5
41	506062.0
42	513758.0
43	511917.5
44	501910.0
45	484132.0
46	457742.0
47	425846.0
48	389914.5
49	351508.0
50	312661.0
51	275083.5
52	240962.0
53	210353.5
54	183765.5
55	161310.5
56	142911.5
57	127986.0
58	115422.5
59	104817.0
60	95804.5
61	87255.5
62	78520.0
63	70254.5
64	62293.0
65	54138.0
66	45961.5
67	38016.0
68	30717.5
69	24231.0
70	18519.0
71	13638.0
72	9761.5
73	6850.0
74	4634.5
75	3082.0
76	1991.0
77	1262.0
78	722.5
79	397.5
80	241.0
81	136.0
82	63.5
83	25.5
84	17.5
85	8.0
86	2.5
87	2.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1763.0
92-93	4060.0
94-95	6466.0
96-97	8895.0
98-99	11260.0
100-101	13783.0
102-103	16445.0
104-105	19078.0
106-107	22016.0
108-109	24970.0
110-111	27495.0
112-113	30189.0
114-115	32261.0
116-117	35376.0
118-119	38193.0
120-121	40922.0
122-123	44078.0
124-125	47075.0
126-127	50702.0
128-129	53502.0
130-131	56648.0
132-133	61904.0
134-135	158725.0
136-137	155754.0
138-139	157788.0
140-141	162189.0
142-143	164632.0
144-145	169655.0
146-147	179531.0
148-149	184016.0
150-151	196420.0
152-153	192784.0
154-155	188803.0
156-157	198258.0
158-159	203602.0
160-161	213555.0
162-163	216650.0
164-165	226367.0
166-167	235421.0
168-169	244383.0
170-171	250029.0
172-173	262274.0
174-175	276198.0
176-177	486211.0
178-179	6032883.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.11664231286254
#Duplication Level	Percentage of total
1	88.86488434175108
2	9.507096036293932
3	1.2512167773207477
4	0.22987542824551063
5	0.06509532024466211
6	0.031668811933374716
7	0.01436466755779645
8	0.006183202140770002
9	0.010350325992778195
>10	0.017978439157079786
>50	0.001286649362305495
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.6308383894393237E-5	0.0	8.769461298131079E-6	8.769461298131079E-6	8.769461298131079E-6	0.0
2	3.5077845192524315E-5	8.769461298131079E-6	2.6308383894393237E-5	2.6308383894393237E-5	8.769461298131079E-6	0.0
3	3.5077845192524315E-5	8.769461298131079E-6	3.5077845192524315E-5	3.5077845192524315E-5	8.769461298131079E-6	0.0
4	4.3847306490655394E-5	2.6308383894393237E-5	4.3847306490655394E-5	3.5077845192524315E-5	8.769461298131079E-6	0.0
5	4.3847306490655394E-5	4.3847306490655394E-5	4.3847306490655394E-5	3.5077845192524315E-5	8.769461298131079E-6	0.0
6	5.261676778878647E-5	6.138622908691755E-5	5.261676778878647E-5	4.3847306490655394E-5	8.769461298131079E-6	0.0
7	5.261676778878647E-5	7.015569038504863E-5	6.138622908691755E-5	4.3847306490655394E-5	8.769461298131079E-6	0.0
8	6.138622908691755E-5	7.892515168317971E-5	6.138622908691755E-5	4.3847306490655394E-5	8.769461298131079E-6	0.0
9	7.892515168317971E-5	7.892515168317971E-5	7.015569038504863E-5	5.261676778878647E-5	8.769461298131079E-6	0.0
10-14	9.471018201981565E-5	1.0874132009682537E-4	1.0172575105832052E-4	8.769461298131079E-5	3.33239529328981E-5	0.0
15-19	1.1400299687570401E-4	1.332958117315924E-4	1.5960419562598564E-4	1.806509027415002E-4	4.910898326953404E-5	0.0
20-24	1.1575688913533024E-4	1.7538922596262158E-4	2.4028323956879158E-4	2.1397485567439833E-4	6.66479058657962E-5	0.0
25-29	1.332958117315924E-4	2.1397485567439833E-4	3.3148563706935475E-4	2.4028323956879152E-4	7.366347490430107E-5	0.0
30-34	2.1046707115514587E-4	2.700994079824372E-4	3.9988743519477716E-4	2.9991557639608287E-4	1.0347964331794673E-4	0.0
35-39	3.788407280792626E-4	3.279778525501023E-4	4.700431255798258E-4	3.788407280792626E-4	1.3855748851047104E-4	1.0523353557757294E-5
40-44	5.805383379362774E-4	3.683173745215053E-4	5.27921570147491E-4	4.139185732717869E-4	1.806509027415002E-4	2.455449163476702E-5
45-49	6.822640889945981E-4	4.314574958680491E-4	6.191239676480541E-4	4.7705869461833067E-4	2.0695928663589346E-4	3.1570060673271885E-5
50-54	8.436221768802097E-4	4.700431255798258E-4	6.857718735138504E-4	5.016131862530977E-4	2.3852934730916536E-4	4.033952197140296E-5
55-59	9.681485273136711E-4	5.401988159648744E-4	7.155880419274961E-4	5.577377385611366E-4	2.841305460594469E-4	5.2616767788786466E-5
60-64	0.0010611048170738606	5.963233682729134E-4	7.47158102600768E-4	6.296473212058114E-4	3.174544989923451E-4	5.61245523080389E-5
65-69	0.0011733539216899384	6.314012134654377E-4	8.190676852454428E-4	7.050646883697387E-4	3.963796506755247E-4	7.015569038504863E-5
70-74	0.002851828814152227	6.647251663983358E-4	8.804539143323603E-4	7.559275638988989E-4	4.665353410605734E-4	7.015569038504863E-5
75-79	0.03409566552713363	6.91033550292729E-4	9.19039544044137E-4	7.839898400529184E-4	5.27921570147491E-4	7.015569038504863E-5
80-84	0.04683067722427958	7.138341496678699E-4	9.699024195732972E-4	8.646688839957244E-4	7.506658871200203E-4	7.892515168317971E-5
85-89	0.04689381734562613	7.383886413026368E-4	9.97964695727317E-4	9.295628976018943E-4	0.0011119676926030207	7.892515168317971E-5
90-94	0.04691135626822239	7.927593013510495E-4	0.0010540892480353557	9.681485273136711E-4	0.0011330143997185353	7.892515168317971E-5
95-99	0.04692714129855903	8.225754697646952E-4	0.0011242449384204043	0.001022519187362084	0.0011487994300551712	7.892515168317971E-5
100-104	0.04694818800567454	8.927311601497439E-4	0.001169846137170686	0.0010751359551508704	0.0011487994300551712	7.892515168317971E-5
105-109	0.0470253592650981	9.330706821211468E-4	0.0012294784739979774	0.0011102138003433945	0.00115406110683405	7.892515168317971E-5
110-114	0.04711305387807941	9.769179886118021E-4	0.00128384913404639	0.001161076675872555	0.001169846137170686	7.892515168317971E-5
115-119	0.0472200413059166	0.0010260269718813362	0.001325942548277419	0.0011979084133247053	0.0011821233829880692	7.892515168317971E-5
120-124	0.047551526942985964	0.0010540892480353555	0.0014031138077009726	0.0012400018275557346	0.001211939551401715	7.892515168317971E-5
125-129	0.04820923654034579	0.0010856593087086276	0.0014592383600090116	0.0012996341643830259	0.001236494043036482	7.892515168317971E-5
130-134	0.04904233536366824	0.0011259988306800306	0.001545179080730696	0.0013522509321718125	0.00126981799596938	7.892515168317971E-5
135-139	0.04995962101545275	0.001155814999093676	0.0016153347711157448	0.00139960602318172	0.0012978802721233997	7.892515168317971E-5
140-144	0.05105931146223839	0.001187385059766948	0.0016398892627505115	0.0014381916528934968	0.0013504970399121862	7.892515168317971E-5
145-149	0.05325167678677116	0.0012154473359209674	0.0016977677073181769	0.0014697617135667688	0.0014101293767394775	7.892515168317971E-5
150-154	0.055187973841398494	0.0012417557198153607	0.001746876690587711	0.0015311479426536863	0.001446961114191628	7.892515168317971E-5
155-159	0.05576851217933478	0.0012803413495271374	0.001804755135155376	0.0015644718955865843	0.0014820389593841522	8.243293620243215E-5
160-164	0.05626837147332825	0.0012996341643830259	0.0018503563339056575	0.0015995497407791087	0.0015434251884710697	9.821796653906809E-5
165-167	0.05658933375683985	0.0013154191947196616	0.0018825110253321382	0.0016048114175579874	0.0015989651100259	1.1400299687570402E-4
>>END_MODULE
