##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479338
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11585903
Total Bases	1.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.35833288091571	39.0	39.0	39.0	37.0	39.0
2	40.092983602572886	41.0	40.0	41.0	38.0	41.0
3	40.11162996962774	41.0	40.0	41.0	38.0	41.0
4	40.10881931257322	41.0	40.0	41.0	38.0	41.0
5	40.095723742896865	41.0	40.0	41.0	38.0	41.0
6	40.0776946777476	41.0	40.0	41.0	38.0	41.0
7	40.103363285537604	41.0	40.0	41.0	38.0	41.0
8	40.08373805649849	41.0	40.0	41.0	38.0	41.0
9	40.05931173426879	41.0	40.0	41.0	38.0	41.0
10-14	39.96530045176453	41.0	40.0	41.0	38.0	41.0
15-19	39.77880619231837	40.4	40.0	41.0	38.0	41.0
20-24	39.59598356727137	40.0	39.0	41.0	37.0	41.0
25-29	39.507458400091906	40.0	39.0	41.0	37.0	41.0
30-34	39.53065595318725	40.0	39.0	41.0	36.8	41.0
35-39	39.28942372467644	40.0	38.8	41.0	35.4	41.0
40-44	38.86881942650478	40.0	37.8	41.0	35.0	41.0
45-49	38.2947201612166	39.4	36.4	41.0	34.4	41.0
50-54	37.640804035732046	38.6	35.2	40.2	34.0	41.0
55-59	36.93535409367747	37.0	35.0	39.6	33.2	41.0
60-64	36.05536835583726	35.8	34.8	38.6	32.6	40.2
65-69	35.65176328508879	35.0	34.6	37.0	32.8	39.6
70-74	35.35711769725674	35.0	34.2	36.4	33.0	39.0
75-79	35.108823403751956	35.0	34.0	36.0	32.8	39.0
80-84	35.01418561850552	35.0	34.0	36.0	32.0	39.6
85-89	34.90265392347924	34.8	33.8	36.0	31.6	40.0
90-94	39.39719347365264	40.6	38.8	40.6	36.8	40.8
95-99	39.59877521497744	40.8	39.0	41.0	37.0	41.0
100-104	39.418617273677306	40.0	39.0	41.0	36.0	41.0
105-109	39.18572058067366	40.0	38.2	41.0	35.4	41.0
110-114	38.9072223011789	40.0	38.0	41.0	35.0	41.0
115-119	38.6968115616023	40.0	38.0	41.0	34.8	41.0
120-124	38.77409273820047	40.0	37.8	41.0	35.0	41.0
125-129	38.20513621488301	39.4	36.6	40.6	33.8	41.0
130-134	38.162874546043824	39.4	36.0	40.8	34.0	41.0
135-139	37.7239664278663	38.8	35.0	40.8	34.0	41.0
140-144	37.095997445275415	37.2	35.0	40.0	33.2	41.0
145-149	36.36964577109428	36.2	35.0	39.0	33.0	40.8
150-154	35.64467747370694	35.0	34.6	37.2	33.0	39.4
155-159	35.01461645477681	35.0	34.0	36.2	32.2	38.0
160-164	34.5288969614493	35.0	34.0	35.4	32.0	36.8
165-169	34.20111335113961	35.0	34.0	35.0	31.6	36.0
170-174	33.987187840457864	35.0	34.0	35.0	31.0	35.6
175-178	33.65538315345944	34.75	33.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	5.0
30	81.0
31	1326.0
32	11076.0
33	59082.0
34	231460.0
35	728696.0
36	1894009.0
37	3781358.0
38	3995848.0
39	877545.0
40	5417.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.571111289297	28.742446747569005	29.309446143300182	21.37699581983381
2	21.281457302033342	28.99753260492514	29.383924584902875	20.337085508138642
3	20.06456466966796	28.4536475059389	30.054532650584076	21.42725517380907
4	20.383478094025126	27.910634156008385	30.546630676952844	21.15925707301364
5	20.88515672882813	28.525269027368864	29.362588310984478	21.22698593281853
6	21.0056911403453	28.604529142009905	29.177794773527793	21.211984944117003
7	20.842656804566722	28.89265515169599	29.17400568604795	21.090682357689342
8	20.81195570168333	29.067039487556556	29.140861959572767	20.980142851187345
9	21.041898935283683	28.983515570603345	28.94726461977111	21.02732087434186
10-14	20.915433177716057	28.917248832482024	29.02951112226643	21.137806867535485
15-19	21.06255161984353	28.75158889212174	28.927771965637895	21.258087522396828
20-24	21.035327155768524	28.730673819727304	28.90332501489094	21.330674009613233
25-29	21.105070532698228	28.70310583473727	28.780425660390907	21.4113979721736
30-34	21.17588244955961	28.708529667476068	28.73396575131002	21.381622131654304
35-39	21.134454517701382	28.701735203548655	28.74722842060735	21.416581858142607
40-44	21.17110940770003	28.697070914541577	28.71763383484222	21.414185842916172
45-49	21.398755021511917	28.579502176049637	28.571283567625244	21.450459234813206
50-54	21.31949663310663	28.646222914174235	28.589533332015638	21.444747120703497
55-59	21.231349856804428	28.650343438918828	28.625608206800973	21.492698497475768
60-64	21.215592776842684	28.66978430597943	28.628385720129025	21.48623719704886
65-69	21.12889431233802	28.679242351675132	28.66942007023535	21.52244326575149
70-74	21.071966509645385	28.71881803256941	28.690946230086684	21.51826922769852
75-79	21.04719330034094	28.695154792854733	28.69053883844876	21.56711306835557
80-84	20.99670780948192	28.722220443240374	28.698291363219596	21.582780384058108
85-89	20.91050477463863	28.70584191840722	28.732967987044255	21.650685319909893
90-94	20.641931431648725	28.615468501652657	29.542769170045986	21.19983089665263
95-99	20.785142954393685	28.98508122027204	29.08536026859841	21.14441555673587
100-104	20.879386071425614	28.900857721250667	28.9872039508934	21.232552256430324
105-109	20.8926940734242	28.85013554415059	28.965182681016355	21.291987701408853
110-114	20.95938406532234	28.77308893885923	28.893081828376932	21.374445167441497
115-119	21.052276240484623	28.79406260490719	28.74991741396015	21.403743740648036
120-124	21.03435585787031	28.835554318318028	28.77042983669253	21.35965998711913
125-129	21.05358743803574	28.844584629418723	28.79128122821645	21.310546704329084
130-134	21.106124765422297	28.883632135576775	28.733286588844592	21.276956510156335
135-139	21.121666863471088	28.889299826418313	28.76384239188159	21.22519091822901
140-144	21.083219159073714	29.005411226957005	28.79077919915399	21.12059041481529
145-149	20.976208924761828	29.09833777960606	28.8745319416376	21.050921353994514
150-154	20.842894052873596	29.22432361540446	28.9714323307821	20.961350000939838
155-159	20.740374078771197	29.35918148423883	29.043692630602397	20.85675180638757
160-164	20.615126637233377	29.5155546464221	29.13727749492665	20.732041221417877
165-169	20.441175877208956	29.635890938678262	29.285479988906634	20.637453195206145
170-174	20.30812528460391	29.8493628946476	29.39150742917876	20.45100439156973
175-178	20.113723855181366	30.007914808858853	29.543012016456032	20.33534931950375
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	2.0
9	9.5
10	22.5
11	54.5
12	127.5
13	277.0
14	519.0
15	1006.0
16	1902.0
17	3438.5
18	5923.0
19	9798.5
20	15619.0
21	23586.0
22	33871.0
23	47050.5
24	63101.0
25	81442.5
26	102628.0
27	126091.0
28	151312.5
29	178470.5
30	207068.0
31	237093.5
32	267114.0
33	297219.5
34	328059.5
35	359864.5
36	391554.0
37	422163.5
38	451178.5
39	476496.0
40	497855.0
41	513235.5
42	520793.5
43	520022.5
44	510273.5
45	492202.0
46	466803.0
47	434843.5
48	397384.5
49	358660.0
50	319798.0
51	281177.5
52	245720.5
53	214609.0
54	188183.0
55	165542.5
56	146627.5
57	131181.5
58	118605.0
59	107899.5
60	98429.0
61	89717.5
62	81038.5
63	72553.0
64	64117.0
65	55854.5
66	47458.5
67	39348.0
68	32183.0
69	25547.5
70	19536.0
71	14491.5
72	10495.5
73	7336.5
74	5009.0
75	3383.5
76	2170.5
77	1328.5
78	809.5
79	471.0
80	273.0
81	144.5
82	75.5
83	46.5
84	21.5
85	8.0
86	5.5
87	3.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1853.0
92-93	4260.0
94-95	6608.0
96-97	9044.0
98-99	11577.0
100-101	13884.0
102-103	16736.0
104-105	19349.0
106-107	22523.0
108-109	25146.0
110-111	27825.0
112-113	30116.0
114-115	33094.0
116-117	35650.0
118-119	38162.0
120-121	41343.0
122-123	44209.0
124-125	47879.0
126-127	51844.0
128-129	55096.0
130-131	57697.0
132-133	62839.0
134-135	168981.0
136-137	157912.0
138-139	165856.0
140-141	166788.0
142-143	169587.0
144-145	174492.0
146-147	178381.0
148-149	186882.0
150-151	198801.0
152-153	191504.0
154-155	197179.0
156-157	199565.0
158-159	205500.0
160-161	213714.0
162-163	218298.0
164-165	232074.0
166-167	241835.0
168-169	253209.0
170-171	264553.0
172-173	269083.0
174-175	279144.0
176-177	486222.0
178-179	6109609.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.11737234355428
#Duplication Level	Percentage of total
1	88.85221241453577
2	9.553745901195176
3	1.2177923277728269
4	0.2369178282222498
5	0.06609492299944199
6	0.033700691781524114
7	0.014358871737603566
8	0.0030901404052900983
9	0.005172362007685026
>10	0.016914539342438317
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.7262357539157716E-5	8.631178769578858E-6	8.631178769578858E-6	1.7262357539157716E-5	8.631178769578858E-6	0.0
2	4.315589384789429E-5	1.7262357539157716E-5	1.7262357539157716E-5	3.452471507831543E-5	8.631178769578858E-6	0.0
3	5.178707261747315E-5	1.7262357539157716E-5	2.5893536308736574E-5	4.315589384789429E-5	1.7262357539157716E-5	0.0
4	5.178707261747315E-5	2.5893536308736574E-5	3.452471507831543E-5	6.041825138705201E-5	1.7262357539157716E-5	0.0
5	5.178707261747315E-5	4.315589384789429E-5	3.452471507831543E-5	6.904943015663086E-5	3.452471507831543E-5	0.0
6	6.904943015663086E-5	4.315589384789429E-5	3.452471507831543E-5	8.631178769578858E-5	4.315589384789429E-5	0.0
7	6.904943015663086E-5	5.178707261747315E-5	5.178707261747315E-5	9.494296646536744E-5	4.315589384789429E-5	0.0
8	8.631178769578858E-5	6.041825138705201E-5	5.178707261747315E-5	1.1220532400452516E-4	4.315589384789429E-5	0.0
9	9.494296646536744E-5	6.904943015663086E-5	5.178707261747315E-5	1.2083650277410401E-4	4.315589384789429E-5	0.0
10-14	1.3119391729759865E-4	1.035741452349463E-4	8.45855519418728E-5	1.7780228265332448E-4	5.869201563313624E-5	0.0
15-19	1.4327756757500902E-4	1.450038033289248E-4	1.2946768154368287E-4	2.433992413021238E-4	6.387072289488354E-5	0.0
20-24	1.639923966219983E-4	1.950646401924822E-4	1.9161216868465063E-4	2.969125496735127E-4	8.113308043404126E-5	0.0
25-29	1.7952851840724024E-4	2.2613688376296607E-4	2.7447148487260765E-4	3.40068443521407E-4	9.494296646536744E-5	0.0
30-34	2.4685171280995535E-4	2.606615988412815E-4	3.711406870918909E-4	3.745931585997225E-4	1.1738403126627248E-4	0.0
35-39	4.22927759709364E-4	3.003650211813442E-4	4.643574178033426E-4	4.108441094319537E-4	1.5708745360633523E-4	3.4524715078315433E-6
40-44	6.318022859331723E-4	3.5560456530664896E-4	5.731102703000361E-4	4.332851742328587E-4	1.8643346142290337E-4	1.898859329307349E-5
45-49	8.199619831099914E-4	4.00486694908459E-4	6.369809931949197E-4	4.6608365355725834E-4	2.485779485638711E-4	2.7619772062652347E-5
50-54	0.0010927072322286834	4.22927759709364E-4	7.198403093828767E-4	5.057870758973211E-4	3.1762737872050197E-4	4.142965809397852E-5
55-59	0.00133092776626906	4.5572623903376364E-4	7.56091260215108E-4	5.454904982373839E-4	3.435209150292385E-4	5.6965779879220455E-5
60-64	0.0014949201628910581	4.937034256199107E-4	8.00973389816918E-4	5.782889775617834E-4	3.9185551613888015E-4	6.214448714096778E-5
65-69	0.0016140304299112466	5.489429697452154E-4	8.579391696961385E-4	6.318022859331724E-4	4.298327027250271E-4	8.113308043404126E-5
70-74	0.0034283042072767223	6.266235786714251E-4	9.010950635440329E-4	7.008517160898033E-4	4.971558971277422E-4	9.494296646536744E-5
75-79	0.036012730298190826	6.887680658123928E-4	9.787756724702426E-4	7.319239596602871E-4	5.662053272843731E-4	1.1047908825060939E-4
80-84	0.05000904979093991	7.129353663672136E-4	0.0010012167372711474	8.113308043404127E-4	7.629962032307711E-4	1.1220532400452516E-4
85-89	0.05010917146466702	7.336501954142029E-4	0.001004669208778979	8.76927762989212E-4	0.0011859239629401352	1.1220532400452516E-4
90-94	0.05019720948811672	7.612699674768554E-4	0.0010236578020720525	9.390722501301798E-4	0.0012100912634949558	1.1220532400452516E-4
95-99	0.05026971138978119	7.854372680316761E-4	0.0010460988668729576	9.701444937006635E-4	0.001216996206510619	1.1220532400452516E-4
100-104	0.05032322469815258	8.320456333874019E-4	0.001066813695919947	0.001015026623302474	0.001216996206510619	1.1738403126627248E-4
105-109	0.05040090530707879	8.855589417587908E-4	0.0011462205406000724	0.0010875285249669362	0.001216996206510619	1.2083650277410403E-4
110-114	0.05048203838751283	9.097262423136117E-4	0.0012066387919871242	0.001141041833338325	0.0012273536210341137	1.2083650277410403E-4
115-119	0.050554540289177295	9.511559004075903E-4	0.0012636045718663446	0.0011600304266313987	0.001248068450081103	1.2083650277410403E-4
120-124	0.050903239911468265	9.82228143978074E-4	0.0013326540020229757	0.0011893764344479666	0.0012722357506359237	1.2083650277410403E-4
125-129	0.05162998516386681	0.0010063954445328949	0.0013758098958708699	0.0012135437350027875	0.0013015817584524918	1.2083650277410403E-4
130-134	0.05231012205090964	0.0010322889808416316	0.0014431330902735851	0.001279140693651587	0.0013326540020229757	1.2083650277410403E-4
135-139	0.05312317909100396	0.001056456281396452	0.001496646398644974	0.0013292015305151443	0.0013568213025777966	1.2083650277410403E-4
140-144	0.05418654031541607	0.0011082433540139252	0.0015380760567389525	0.0013947984891639436	0.0013792623673787017	1.2083650277410403E-4
145-149	0.056370228544119515	0.001149673012107904	0.0016088517226494994	0.00142069202547268	0.0014224182612265956	1.2946768154368287E-4
150-154	0.05812753654160578	0.0011755665484166405	0.0016623650310208884	0.0014396806187657535	0.0014759315695979844	1.2946768154368287E-4
155-159	0.05865058597504226	0.0011876501986940507	0.001691711038837456	0.0014828365126136477	0.0015018251059067214	1.2946768154368287E-4
160-164	0.059163277993955245	0.0012083650277410402	0.0017348669326853502	0.0015104562846763002	0.001519087463445879	1.2946768154368287E-4
165-167	0.05950910055665637	0.001240012683229496	0.001769391647763666	0.0015305957018053172	0.0015190874634458788	1.2946768154368287E-4
>>END_MODULE
