##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479366
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9766442
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.29875895438687	39.0	39.0	39.0	37.0	39.0
2	39.99898755350208	41.0	40.0	41.0	38.0	41.0
3	40.02861451488679	41.0	40.0	41.0	38.0	41.0
4	40.02763862213076	41.0	40.0	41.0	38.0	41.0
5	40.03097473982849	41.0	40.0	41.0	38.0	41.0
6	40.012898146530745	41.0	40.0	41.0	38.0	41.0
7	40.033788354039274	41.0	40.0	41.0	38.0	41.0
8	40.00976015625752	41.0	40.0	41.0	38.0	41.0
9	39.98149326028865	41.0	40.0	41.0	38.0	41.0
10-14	39.89323090230813	41.0	40.0	41.0	38.0	41.0
15-19	39.835377694353795	40.6	40.0	41.0	38.0	41.0
20-24	39.84127120193823	41.0	40.0	41.0	38.0	41.0
25-29	39.69677950270938	40.6	39.8	41.0	37.2	41.0
30-34	39.467517566786356	40.0	39.0	41.0	36.4	41.0
35-39	39.134672463114	40.0	38.2	41.0	35.2	41.0
40-44	38.70397702663877	40.0	37.4	41.0	35.0	41.0
45-49	38.283227935004376	39.4	36.4	41.0	34.8	41.0
50-54	37.61706650180281	38.2	35.2	40.2	34.0	41.0
55-59	36.85545829279486	36.6	35.0	39.4	33.8	41.0
60-64	35.84766720572343	35.4	34.6	38.0	32.4	40.0
65-69	35.44083237273103	35.0	34.6	36.8	32.8	39.0
70-74	35.259113052634724	35.0	35.0	36.0	33.0	38.8
75-79	35.01684791656982	35.0	34.0	35.6	33.0	37.6
80-84	34.92003753260399	35.0	34.0	35.0	33.0	37.8
85-89	34.59987141683737	34.8	33.4	35.0	31.8	38.4
90-94	39.4747597539616	40.6	39.8	40.6	37.0	40.8
95-99	39.67756092551164	41.0	39.4	41.0	37.0	41.0
100-104	39.52492542887075	40.4	39.0	41.0	36.6	41.0
105-109	39.50996207879291	41.0	39.0	41.0	36.0	41.0
110-114	39.379384752763364	40.2	39.0	41.0	35.8	41.0
115-119	39.18043028039093	40.0	38.2	41.0	35.0	41.0
120-124	38.91569644301268	40.0	38.0	41.0	35.0	41.0
125-129	38.2962014762914	39.4	37.2	40.4	34.4	40.8
130-134	38.38497918262466	39.6	36.6	40.8	34.6	41.0
135-139	37.948317979298935	39.0	35.4	41.0	34.0	41.0
140-144	37.279075137665664	37.4	35.0	40.2	34.0	41.0
145-149	36.55309176726418	36.2	35.0	39.0	33.8	41.0
150-154	35.84426671470239	35.0	35.0	37.2	33.0	39.6
155-159	35.264301925924094	35.0	35.0	36.2	33.0	38.4
160-164	34.82634316934537	35.0	34.4	35.4	33.0	36.8
165-169	34.54108260738876	35.0	34.0	35.0	33.0	36.0
170-174	34.35035150075207	35.0	34.0	35.0	32.8	36.0
175-178	33.82654866139804	34.75	33.25	35.0	31.25	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	160.0
31	2134.0
32	15823.0
33	73093.0
34	235895.0
35	604545.0
36	1447995.0
37	3103051.0
38	3411482.0
39	853037.0
40	19224.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.466814629114676	28.53387139349212	28.89020382243605	21.109110154957147
2	21.62473293754266	28.928016978957128	28.87908411271986	20.568165970780353
3	20.35928744572486	28.506881011529074	29.79759670922123	21.336234833524838
4	20.714626677760435	27.855722687955346	30.028387001120777	21.40126363316344
5	21.23255326760759	28.42626823565839	29.076781493198855	21.264397003535166
6	21.059010026373986	28.521645856290345	29.161274904412476	21.258069212923193
7	21.049323796731706	28.471525249420417	28.955846970677758	21.523303983170123
8	21.273673667442043	28.593975165162504	28.735080800152197	21.39727036724326
9	21.235481662615722	28.67661529142343	28.751309842417534	21.336593203543316
10-14	21.36551878360615	28.584422044384226	28.516630723860338	21.533428448149287
15-19	21.419272238549105	28.45935090793556	28.548277868234923	21.57309898528041
20-24	21.453241620643425	28.371769371077	28.54041011045783	21.634578897821747
25-29	21.529070668724597	28.34813947597293	28.399843054410194	21.722946800892277
30-34	21.526457639332726	28.379147697800285	28.373028785713366	21.721365877153627
35-39	21.519775574359628	28.38854927925646	28.356291881936123	21.735383264447787
40-44	21.59427353380074	28.32906395184654	28.271857857754135	21.804804656598588
45-49	21.757581727306626	28.26071152626514	28.197462289746873	21.78424445668136
50-54	21.73881337748179	28.302089952512897	28.215737112860552	21.74335955714476
55-59	21.695219200605504	28.281523609109644	28.195629483080943	21.827627707203913
60-64	21.632561786574886	28.311739321238992	28.249092146351764	21.806606745834358
65-69	21.643073291174	28.346065025523114	28.221722916083465	21.789138767219423
70-74	21.599405392465343	28.348278728322967	28.20541400849972	21.846901870711974
75-79	21.56336155992121	28.377900570135985	28.223592583665575	21.835145286277232
80-84	21.55391697406282	28.432811048281454	28.18499101310385	21.82828096455188
85-89	21.50634181823841	28.419129504890318	28.13793395793473	21.93659471893654
90-94	21.05266725961541	28.501056407563794	29.192590765049296	21.253685567771498
95-99	21.10060797295948	28.589007450369646	28.791399284913755	21.518985291757115
100-104	21.281807858405312	28.590620626794255	28.538084656227213	21.589486858573217
105-109	21.319925506460454	28.447562196200526	28.5507102013373	21.681802096001725
110-114	21.3899268202843	28.393861087643295	28.488067733253903	21.728144358818504
115-119	21.45673296268533	28.41097238148846	28.3768966110335	21.75539804479271
120-124	21.458819417755922	28.42644085171479	28.32025280108701	21.79448692944228
125-129	21.50373401387307	28.39731616035322	28.32979306272545	21.769156763048258
130-134	21.598452185708393	28.377183374411857	28.27029462771548	21.75406981216427
135-139	21.647698519626754	28.37604108928849	28.22441849369361	21.75184189739115
140-144	21.62690561560147	28.42076215508239	28.254247994010818	21.69808423530532
145-149	21.582646652105453	28.483227897596088	28.294158141939395	21.63996730835906
150-154	21.516479007558175	28.550456640509303	28.310488449349165	21.622575902583357
155-159	21.45415983167571	28.619148862457134	28.368299003351865	21.558392302515294
160-164	21.4029493590308	28.72466003382204	28.390333393043104	21.482057214104056
165-169	21.327332849010112	28.801676364202134	28.433680875071893	21.437309911715857
170-174	21.244831329547083	28.941879113984932	28.46962661928612	21.343662937181865
175-178	21.184744890888553	29.05380798659215	28.492428150634435	21.269018971884865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	2.5
11	6.0
12	25.0
13	74.5
14	219.0
15	519.5
16	1077.5
17	2114.5
18	3840.5
19	6634.5
20	10834.5
21	16903.0
22	24912.0
23	34877.0
24	46927.5
25	60599.5
26	75662.5
27	92178.0
28	110206.0
29	129495.0
30	149560.5
31	171000.5
32	193371.5
33	217258.5
34	242780.5
35	268563.0
36	295189.5
37	323580.5
38	352715.0
39	379296.0
40	402896.0
41	422962.0
42	437323.5
43	444588.5
44	445000.5
45	437663.5
46	422103.0
47	400897.5
48	374133.5
49	344078.5
50	313008.5
51	280973.5
52	248715.0
53	218161.5
54	190719.5
55	166018.0
56	144690.0
57	126318.5
58	109967.0
59	95691.5
60	83782.0
61	73490.0
62	64614.0
63	56447.5
64	48757.0
65	41885.0
66	35526.5
67	29598.5
68	24163.0
69	19223.0
70	14953.5
71	11503.5
72	8485.5
73	6112.5
74	4358.5
75	2967.0
76	1956.5
77	1280.5
78	781.5
79	474.0
80	268.0
81	136.5
82	74.0
83	42.5
84	24.0
85	13.0
86	5.5
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1098.0
92-93	2568.0
94-95	3888.0
96-97	5384.0
98-99	6526.0
100-101	7811.0
102-103	9003.0
104-105	10442.0
106-107	11459.0
108-109	13018.0
110-111	14281.0
112-113	15494.0
114-115	16788.0
116-117	17791.0
118-119	19117.0
120-121	20295.0
122-123	21784.0
124-125	23173.0
126-127	24762.0
128-129	26256.0
130-131	27990.0
132-133	31800.0
134-135	96324.0
136-137	100230.0
138-139	98951.0
140-141	99425.0
142-143	105150.0
144-145	105310.0
146-147	105818.0
148-149	106778.0
150-151	113714.0
152-153	116880.0
154-155	113979.0
156-157	116435.0
158-159	121205.0
160-161	128037.0
162-163	137619.0
164-165	138280.0
166-167	146118.0
168-169	153412.0
170-171	162319.0
172-173	167855.0
174-175	179660.0
176-177	737343.0
178-179	6084872.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.85449304247759
#Duplication Level	Percentage of total
1	85.95670257729364
2	9.323515537640573
3	2.236690169516206
4	1.0769367690582292
5	0.564673176534205
6	0.30222282955073226
7	0.15960664205141042
8	0.11074221470210342
9	0.07073667759860547
>10	0.1771075928462986
>50	0.005393270052882003
>100	0.003400836398662524
>500	0.012271706756449141
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.023914338507309E-5	0.0	0.0	0.0
2	7.167400369551163E-5	0.0	2.047828677014618E-5	3.071743015521927E-5	0.0	0.0
3	7.167400369551163E-5	1.023914338507309E-5	2.047828677014618E-5	3.071743015521927E-5	0.0	0.0
4	8.191314708058472E-5	1.023914338507309E-5	2.047828677014618E-5	5.119571692536545E-5	1.023914338507309E-5	0.0
5	1.023914338507309E-4	1.023914338507309E-5	2.047828677014618E-5	6.143486031043854E-5	1.023914338507309E-5	0.0
6	1.023914338507309E-4	1.023914338507309E-5	3.071743015521927E-5	7.167400369551163E-5	1.023914338507309E-5	0.0
7	1.023914338507309E-4	2.047828677014618E-5	4.095657354029236E-5	7.167400369551163E-5	1.023914338507309E-5	0.0
8	1.023914338507309E-4	3.071743015521927E-5	4.095657354029236E-5	7.167400369551163E-5	1.023914338507309E-5	0.0
9	1.023914338507309E-4	3.071743015521927E-5	4.095657354029236E-5	7.167400369551163E-5	2.047828677014618E-5	0.0
10-14	1.2696537797490633E-4	4.5052230894321595E-5	4.5052230894321595E-5	1.0034360517371629E-4	3.071743015521927E-5	0.0
15-19	1.494914934220671E-4	8.191314708058473E-5	6.143486031043854E-5	1.4949149342206712E-4	4.5052230894321595E-5	0.0
20-24	1.6587412283818403E-4	1.0443926252774551E-4	6.143486031043854E-5	1.7816109490027175E-4	7.576966104954087E-5	0.0
25-29	1.8430458093131563E-4	1.2082189194386245E-4	7.781748972655548E-5	2.170698397635495E-4	9.829577649670166E-5	0.0
30-34	2.5393075594981265E-4	1.2286972062087708E-4	9.624794781968706E-5	2.3550029785668106E-4	1.3925235003699402E-4	6.143486031043854E-6
35-39	4.1775705011098203E-4	1.4334800739102327E-4	1.12630577235804E-4	2.723612140429442E-4	2.3140464050265186E-4	2.4573944124175415E-5
40-44	6.184442604584147E-4	1.5358715077609634E-4	1.249175492978917E-4	3.051264728751781E-4	3.64513504508602E-4	4.095657354029236E-5
45-49	7.392661524022772E-4	1.82256752254301E-4	1.4130017871400867E-4	3.6041784715457276E-4	4.648571096823183E-4	6.553051766446779E-5
50-54	8.252749568368912E-4	2.1297418240952027E-4	1.474436647450525E-4	4.136613927569529E-4	6.041094597193123E-4	9.010446178864319E-5
55-59	9.071881039174757E-4	2.355002978566811E-4	1.6996978019221328E-4	4.6690493835933286E-4	6.860226067998971E-4	1.1672623458983322E-4
60-64	9.993403943831336E-4	2.477872699187688E-4	1.9863938167041795E-4	4.935267111605229E-4	7.454096384333209E-4	1.2901320665192092E-4
65-69	0.0011119709716189378	2.7440904271995884E-4	2.3550029785668106E-4	5.447224280858883E-4	8.047966700667449E-4	1.3925235003699405E-4
70-74	0.0021993679991136996	3.071743015521927E-4	2.498350985957834E-4	5.959181450112537E-4	8.662315303771834E-4	1.4949149342206712E-4
75-79	0.025073614321366983	3.174134449372658E-4	2.7645687139697347E-4	6.245877464894584E-4	9.297142193646366E-4	1.5358715077609634E-4
80-84	0.03620970666697247	3.522265324465143E-4	2.969351581671196E-4	6.368747185515462E-4	0.0010116273664452214	1.5358715077609634E-4
85-89	0.03628957198537605	3.7065699053964583E-4	3.1331778758323655E-4	6.778312920918386E-4	0.001243032006947873	1.5358715077609634E-4
90-94	0.03630800244346918	4.0137442069486514E-4	3.174134449372658E-4	7.228835229861601E-4	0.0012676059510720486	1.5358715077609634E-4
95-99	0.03632028941553127	4.1570922143396746E-4	3.3584390303039737E-4	7.536009531413794E-4	0.0012901320665192095	1.5358715077609634E-4
100-104	0.0363530546743635	4.3413967952709903E-4	3.4813087509248506E-4	7.863662119736133E-4	0.001318801667997414	1.5358715077609634E-4
105-109	0.036416537363350945	4.6895276703634754E-4	3.686091618626312E-4	8.232271281598764E-4	0.0013474712694756187	1.5358715077609634E-4
110-114	0.03647592439498437	4.7714408174440604E-4	3.829439626017336E-4	8.539445583150958E-4	0.0013638538988917357	1.5358715077609634E-4
115-119	0.036539407083971825	5.037658545455961E-4	3.8908744863277745E-4	8.785185024392711E-4	0.0014089061297860573	1.5358715077609634E-4
120-124	0.03668070726268584	5.262919699927568E-4	3.9318310598680664E-4	9.194750759795635E-4	0.0014560061893573934	1.5358715077609634E-4
125-129	0.03702679030910131	5.426745994088738E-4	4.1775705011098203E-4	9.563359921658266E-4	0.0015440628224690218	1.5358715077609634E-4
130-134	0.037399495128317965	5.426745994088738E-4	4.198048787879967E-4	9.993403943831336E-4	0.0016177846548415483	1.5358715077609634E-4
135-139	0.0378664000666773	5.590572288249907E-4	4.423309942351575E-4	0.0010484882826314844	0.0017017456305991475	1.5358715077609634E-4
140-144	0.03824934402927903	5.733920295640931E-4	4.546179662972452E-4	0.001103779656910879	0.0017652283195866009	1.5973063680714019E-4
145-149	0.03853399221538407	5.79535515595137E-4	4.730484243903768E-4	0.0011467840591281862	0.0018246153512200248	1.6382629416116944E-4
150-154	0.03871624896763837	5.856790016261807E-4	4.935267111605229E-4	0.0011979797760535516	0.0019290546137477702	1.6587412283818406E-4
155-159	0.03891898400666281	6.06157288396327E-4	5.221963126387276E-4	0.0012348406922398146	0.002010967760828355	1.7406543754624253E-4
160-164	0.03933264539941977	6.245877464894585E-4	5.447224280858883E-4	0.001253271150332946	0.0020887852505549103	1.7406543754624253E-4
165-167	0.039748354620853736	6.280007942844829E-4	5.6315288617902E-4	0.001266240731954039	0.002160459254250422	1.7406543754624253E-4
>>END_MODULE
