##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479367
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9829270
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.3068370285891	39.0	39.0	39.0	37.0	39.0
2	40.00390395217549	41.0	40.0	41.0	38.0	41.0
3	40.026640025149376	41.0	40.0	41.0	38.0	41.0
4	40.02657664302639	41.0	40.0	41.0	38.0	41.0
5	40.02378233581945	41.0	40.0	41.0	38.0	41.0
6	40.00249713356129	41.0	40.0	41.0	38.0	41.0
7	40.0269447273297	41.0	40.0	41.0	38.0	41.0
8	40.004556289531166	41.0	40.0	41.0	38.0	41.0
9	39.97448386299288	41.0	40.0	41.0	38.0	41.0
10-14	39.890479740611454	41.0	40.0	41.0	38.0	41.0
15-19	39.820108390551894	40.8	40.0	41.0	38.0	41.0
20-24	39.8093133467694	41.0	40.0	41.0	38.0	41.0
25-29	39.66210485621007	40.6	39.4	41.0	37.0	41.0
30-34	39.43733854090893	40.0	39.0	41.0	36.4	41.0
35-39	39.10786038027239	40.0	38.0	41.0	35.2	41.0
40-44	38.7066316013295	40.0	37.4	41.0	35.0	41.0
45-49	38.319049125723474	39.4	36.4	41.0	34.8	41.0
50-54	37.66356472047263	38.2	35.2	40.4	34.0	41.0
55-59	36.903112357275766	36.8	35.0	39.4	34.0	41.0
60-64	35.9029251612785	35.6	34.6	38.2	32.4	40.2
65-69	35.50298475878677	35.0	34.6	37.0	32.8	39.0
70-74	35.310717459180594	35.0	35.0	36.0	33.0	39.0
75-79	35.06353442320742	35.0	34.4	35.8	33.0	38.0
80-84	34.95469728677714	35.0	34.0	35.0	33.0	38.0
85-89	34.648540776680264	34.8	33.4	35.0	32.2	38.4
90-94	39.42393113263553	40.6	39.4	40.6	36.8	40.8
95-99	39.65022690732209	41.0	39.2	41.0	37.0	41.0
100-104	39.49457625600968	40.4	39.0	41.0	36.4	41.0
105-109	39.4640477950367	40.6	39.0	41.0	36.0	41.0
110-114	39.32582151694511	40.0	38.8	41.0	35.6	41.0
115-119	39.118355832534746	40.0	38.0	41.0	35.0	41.0
120-124	38.84417631877223	40.0	38.0	41.0	35.0	41.0
125-129	38.2364405449603	39.4	36.6	40.4	34.4	40.8
130-134	38.308287938140566	39.6	36.4	40.8	34.0	41.0
135-139	37.85561881088928	38.8	35.2	41.0	34.0	41.0
140-144	37.18074796204786	37.2	35.0	39.8	34.0	41.0
145-149	36.441829502214624	36.0	35.0	38.8	33.4	40.8
150-154	35.73694529325699	35.0	35.0	37.0	33.0	39.4
155-159	35.16782994327377	35.0	35.0	36.0	33.0	38.0
160-164	34.75227806180804	35.0	34.0	35.2	33.0	36.6
165-169	34.47603669409936	35.0	34.0	35.0	33.0	36.0
170-174	34.29861518148253	35.0	34.0	35.0	32.0	36.0
175-178	33.8106439293401	34.75	33.25	35.0	31.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	174.0
31	2205.0
32	16264.0
33	75277.0
34	244833.0
35	627762.0
36	1487542.0
37	3140980.0
38	3384247.0
39	832172.0
40	17808.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.442558806503435	28.536747896842797	28.929188027188186	21.091505269465586
2	21.584980369854527	28.965253777747485	28.929371153707244	20.520394698690748
3	20.31805006882505	28.600842178513762	29.809426335831652	21.27168141682953
4	20.679755465054882	27.896598628382375	30.102408418936506	21.32123748762624
5	21.187341481106937	28.464514658769165	29.102853009429996	21.245290850693895
6	21.011499328027412	28.571857320024783	29.19550485437881	21.221138497568994
7	21.019607763343565	28.493733512254728	28.99656841250673	21.49009031189498
8	21.228473732026895	28.621474432994514	28.779237929164626	21.37081390581396
9	21.22436355904355	28.668751595998483	28.788333212944607	21.318551632013364
10-14	21.329929893064286	28.627336516343533	28.548197373762243	21.49453621682994
15-19	21.381003879230096	28.480713216749564	28.5850464988753	21.55323640514504
20-24	21.43185200935573	28.414893476321236	28.56961707227495	21.583637442048087
25-29	21.49026529945764	28.385104895887487	28.428658486337238	21.695971318317635
30-34	21.48854594491758	28.426414169109197	28.399398938069666	21.685640947903558
35-39	21.48029914734258	28.43939987404965	28.379104450279623	21.701196528328147
40-44	21.57126012409874	28.341579791785147	28.295769675672762	21.791390408443352
45-49	21.736714934069365	28.283131911118524	28.224187554111342	21.755965600700762
50-54	21.714149677442983	28.33938430829553	28.231382391571298	21.71508362269019
55-59	21.67062050386244	28.30527597675107	28.229661002292133	21.79444251709435
60-64	21.61990666651745	28.34595855032978	28.25848308165306	21.775651701499704
65-69	21.62038483020611	28.377863259428217	28.247485316813965	21.75426659355171
70-74	21.588396696804544	28.381485095027404	28.219525966831714	21.810592241336334
75-79	21.547252237449985	28.41788454279921	28.236573011017096	21.79829020873371
80-84	21.538814174399523	28.476226617032594	28.17989535336805	21.805063855199826
85-89	21.48326579695135	28.46162329450712	28.154339030263692	21.90077187827784
90-94	21.02093435071379	28.53892402136283	29.223371476790703	21.216770151132668
95-99	21.052125434787726	28.645095987623925	28.810081829873347	21.492696747715005
100-104	21.242963048313666	28.625402427484175	28.566433572855658	21.5652009513465
105-109	21.276698716012543	28.503456228176542	28.57143441818495	21.648410637625968
110-114	21.34070000783339	28.422753739972222	28.5314744000277	21.705071852166686
115-119	21.40949665549338	28.467330393103957	28.402415657520873	21.72075729388179
120-124	21.4179620658234	28.449437715626903	28.355007883084394	21.77759233546531
125-129	21.46713111645922	28.426277773044013	28.362075540834454	21.744515569662312
130-134	21.55383513697732	28.421541423527113	28.29983951128119	21.72478392821438
135-139	21.62002128583995	28.40516056556821	28.249764152086797	21.725053996505046
140-144	21.590286863463533	28.459139242504776	28.287979510544137	21.662594383487555
145-149	21.527776449836463	28.529984444419554	28.323565452315176	21.618673653428807
150-154	21.487927561673516	28.583833288327554	28.340766762392533	21.587472387606397
155-159	21.429567728225603	28.64977346202966	28.39608715418086	21.52457165556388
160-164	21.335846009936972	28.775723068344945	28.439720674281222	21.448710247436868
165-169	21.26440149309697	28.858801908661242	28.462649713804815	21.41414688443697
170-174	21.18912484310921	29.00347493561717	28.527440247897417	21.27995997337621
175-178	21.10509955235638	29.119265656913473	28.55258373860254	21.2230510521276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.0
11	6.5
12	29.5
13	93.5
14	230.5
15	530.0
16	1123.5
17	2133.5
18	3908.0
19	6719.5
20	10929.5
21	17012.0
22	25181.0
23	35526.0
24	47758.0
25	61545.5
26	76635.5
27	93370.5
28	111666.0
29	131137.5
30	151930.5
31	173590.0
32	196231.0
33	219445.0
34	244457.5
35	271884.5
36	299915.0
37	328513.0
38	356448.5
39	382951.0
40	407055.0
41	426897.5
42	441485.0
43	448654.0
44	448521.5
45	440902.5
46	424744.5
47	402824.5
48	376318.5
49	345801.0
50	313831.5
51	280783.0
52	248598.5
53	218028.0
54	190095.0
55	165767.0
56	144022.5
57	125610.0
58	109223.5
59	95050.0
60	83242.5
61	73082.0
62	63970.5
63	55622.5
64	48293.0
65	41443.0
66	35077.0
67	29090.0
68	23727.5
69	19061.0
70	14793.5
71	11281.0
72	8370.0
73	5960.0
74	4255.5
75	2911.5
76	1903.5
77	1203.5
78	732.5
79	444.0
80	253.5
81	129.5
82	76.0
83	47.5
84	19.5
85	6.5
86	2.0
87	1.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1121.0
92-93	2754.0
94-95	4134.0
96-97	5612.0
98-99	6842.0
100-101	8443.0
102-103	9619.0
104-105	10981.0
106-107	12295.0
108-109	14177.0
110-111	15188.0
112-113	16401.0
114-115	17826.0
116-117	18842.0
118-119	20537.0
120-121	21856.0
122-123	23219.0
124-125	24896.0
126-127	26130.0
128-129	27610.0
130-131	29535.0
132-133	33137.0
134-135	101546.0
136-137	105760.0
138-139	104418.0
140-141	104549.0
142-143	110343.0
144-145	110512.0
146-147	110099.0
148-149	114261.0
150-151	120849.0
152-153	123434.0
154-155	116571.0
156-157	121157.0
158-159	125318.0
160-161	131485.0
162-163	140078.0
164-165	142640.0
166-167	150556.0
168-169	156709.0
170-171	159943.0
172-173	168943.0
174-175	175213.0
176-177	616286.0
178-179	6167445.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.79778538909316
#Duplication Level	Percentage of total
1	85.8559180425324
2	9.420678045158326
3	2.2133655791192823
4	1.0585908756703082
5	0.5462604513947769
6	0.35953867269708223
7	0.17913789661631524
8	0.10657893551314435
9	0.07071885187983963
>10	0.17259769491791901
>50	0.00272975021569379
>100	0.0016217627071149266
>500	0.012263441577760225
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.0521086509984975E-5	0.0	0.0	1.0173695503328324E-5	0.0	0.0
2	7.121586852329827E-5	1.0173695503328324E-5	1.0173695503328324E-5	1.0173695503328324E-5	1.0173695503328324E-5	0.0
3	7.121586852329827E-5	4.06947820133133E-5	1.0173695503328324E-5	1.0173695503328324E-5	3.0521086509984975E-5	0.0
4	7.121586852329827E-5	4.06947820133133E-5	2.034739100665665E-5	2.034739100665665E-5	4.06947820133133E-5	0.0
5	7.121586852329827E-5	5.086847751664162E-5	2.034739100665665E-5	3.0521086509984975E-5	4.06947820133133E-5	0.0
6	7.121586852329827E-5	6.104217301996995E-5	2.034739100665665E-5	4.06947820133133E-5	5.086847751664162E-5	0.0
7	8.13895640266266E-5	6.104217301996995E-5	2.034739100665665E-5	6.104217301996995E-5	6.104217301996995E-5	0.0
8	9.156325952995492E-5	6.104217301996995E-5	2.034739100665665E-5	8.13895640266266E-5	6.104217301996995E-5	0.0
9	9.156325952995492E-5	6.104217301996995E-5	2.034739100665665E-5	8.13895640266266E-5	6.104217301996995E-5	0.0
10-14	9.359799863062058E-5	7.325060762396394E-5	2.2382130107322313E-5	1.0784117233528024E-4	9.563273773128625E-5	0.0
15-19	1.261538242412712E-4	1.0173695503328324E-4	3.866004291264763E-5	1.4853595434859355E-4	1.3022330244260254E-4	0.0
20-24	1.4650121524792788E-4	1.1598012873794288E-4	4.67989993153103E-5	1.973696927645695E-4	1.627791280532532E-4	0.0
25-29	1.5260543254992488E-4	1.3429278064393388E-4	6.104217301996995E-5	2.3602973567721716E-4	1.7498756265724717E-4	0.0
30-34	2.0347391006656648E-4	1.5870964985192186E-4	6.91811294226326E-5	2.645160830865364E-4	2.1364760556989482E-4	0.0
35-39	3.88635168227142E-4	1.7295282355658153E-4	1.1598012873794291E-4	3.011413868985184E-4	2.991066477978528E-4	4.069478201331329E-6
40-44	5.961785564950398E-4	2.0347391006656648E-4	1.261538242412712E-4	3.6625303811981967E-4	3.6218355991848834E-4	1.627791280532532E-5
45-49	7.141934243336483E-4	2.3196025747588582E-4	1.322580415432682E-4	4.089825592337987E-4	4.496773412471119E-4	3.0521086509984975E-5
50-54	8.261040748702599E-4	2.5841186578453944E-4	1.322580415432682E-4	4.517120803477776E-4	5.636227308843892E-4	5.086847751664162E-5
55-59	9.034241606955554E-4	2.746897785898648E-4	1.444664761472622E-4	4.8223316685776257E-4	6.205954257030279E-4	7.325060762396394E-5
60-64	0.0010051611157288385	2.9503716959652145E-4	1.7702230175791286E-4	5.046152969650849E-4	6.938460333269917E-4	9.156325952995492E-5
65-69	0.0010784117233528024	3.235235170058407E-4	2.0347391006656648E-4	5.392058616764011E-4	7.467492499442991E-4	9.156325952995492E-5
70-74	0.0022239698370275715	3.6218355991848834E-4	2.0957812736856346E-4	5.758311654883832E-4	8.159303793669317E-4	1.1394538963727723E-4
75-79	0.025039499372791672	3.927046464284733E-4	2.2382130107322313E-4	6.043175128977024E-4	8.973199433935581E-4	1.4446647614726218E-4
80-84	0.03552247521942118	4.1712151563646127E-4	2.3399499657655143E-4	6.348385994076874E-4	0.0011272454617687784	1.688833453552502E-4
85-89	0.035605899522548466	4.395036457437836E-4	2.4009921387854846E-4	6.734986423203351E-4	0.0014182131531639685	1.7295282355658153E-4
90-94	0.035628281652655795	4.598510367504403E-4	2.584118657845394E-4	6.91811294226326E-4	0.0014446647614726222	1.7295282355658153E-4
95-99	0.03563235113085712	4.92406862361091E-4	2.6858556128786775E-4	7.060544679309856E-4	0.0014568731960766161	1.7295282355658153E-4
100-104	0.03567101117376977	5.086847751664162E-4	2.9300243049585576E-4	7.365755544409707E-4	0.0014833248043852698	1.7295282355658153E-4
105-109	0.03571170595578309	5.086847751664162E-4	2.950371695965214E-4	7.487839890449647E-4	0.0015016374562912607	1.7295282355658153E-4
110-114	0.03576460917240039	5.086847751664162E-4	3.0521086509984976E-4	7.71166119152287E-4	0.001542332238304574	1.7295282355658153E-4
115-119	0.035795130258910374	5.086847751664162E-4	3.1131508240184674E-4	7.813398146556154E-4	0.0015687838466132277	1.7295282355658153E-4
120-124	0.03589890195304432	5.127542533677475E-4	3.357319516098347E-4	8.098261620649346E-4	0.0016420344542371914	1.7295282355658153E-4
125-129	0.03614307064512421	5.290321661730728E-4	3.6218355991848834E-4	8.484862049775822E-4	0.0017193545400624867	1.7295282355658153E-4
130-134	0.036499149987740696	5.371711225757355E-4	3.6625303811981967E-4	9.034241606955552E-4	0.0017661535393777975	1.7295282355658153E-4
135-139	0.03693254941618248	5.737964263877176E-4	3.7235725542181664E-4	9.603968555141939E-4	0.0018027788431897792	1.7295282355658153E-4
140-144	0.03746361632145622	5.839701218910458E-4	3.8660042912647634E-4	0.0010316127240374922	0.0019045157982230624	1.7295282355658153E-4
145-149	0.037781035621160065	5.900743391930428E-4	3.9677412462980465E-4	0.0010600990714468115	0.0019431758411357098	1.749875626572472E-4
150-154	0.03802316957413927	5.961785564950398E-4	3.9677412462980465E-4	0.0010845159406547994	0.0019960790577530176	1.8312651905990984E-4
155-159	0.03836093626484978	6.104217301996995E-4	4.00843602831136E-4	0.0011089328098627875	0.0020591559698736528	1.8312651905990984E-4
160-164	0.03878416199778824	6.144912084010308E-4	4.089825592337986E-4	0.001141488635473438	0.0021100244473902944	1.8312651905990984E-4
165-167	0.03911446797846296	6.205954257030278E-4	4.306864429742324E-4	0.0011699749828827574	0.002136476055698948	1.831265190599098E-4
>>END_MODULE
