##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479368
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9955469
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.261000963390075	39.0	39.0	39.0	37.0	39.0
2	39.95694356539104	41.0	40.0	41.0	38.0	41.0
3	39.984708907234804	41.0	40.0	41.0	38.0	41.0
4	39.98341956566787	41.0	40.0	41.0	38.0	41.0
5	39.98033030889856	41.0	40.0	41.0	38.0	41.0
6	39.961739522266605	41.0	40.0	41.0	38.0	41.0
7	39.984325198541626	41.0	40.0	41.0	38.0	41.0
8	39.96334667909669	41.0	40.0	41.0	38.0	41.0
9	39.93521370012804	41.0	40.0	41.0	38.0	41.0
10-14	39.85714901025758	41.0	40.0	41.0	38.0	41.0
15-19	39.78999713624742	40.8	40.0	41.0	38.0	41.0
20-24	39.772461066374674	41.0	40.0	41.0	37.8	41.0
25-29	39.636079706541196	40.4	39.4	41.0	37.0	41.0
30-34	39.4189792766167	40.0	39.0	41.0	36.2	41.0
35-39	39.10307839841598	40.0	38.0	41.0	35.2	41.0
40-44	38.70704729229733	40.0	37.4	41.0	35.0	41.0
45-49	38.31656684381218	39.4	36.4	41.0	34.8	41.0
50-54	37.67215093533012	38.2	35.2	40.4	34.0	41.0
55-59	36.91851749023577	36.8	35.0	39.4	34.0	41.0
60-64	35.93752645907491	35.6	34.6	38.2	32.6	40.2
65-69	35.51639333114291	35.0	34.6	37.0	32.8	39.0
70-74	35.30603649109851	35.0	35.0	36.0	33.0	38.8
75-79	35.05955353785944	35.0	34.4	35.8	33.0	37.6
80-84	34.94452271409815	35.0	34.0	35.0	33.0	37.4
85-89	34.64757782882956	34.8	33.6	35.0	32.2	38.2
90-94	39.372019461705534	40.6	38.8	40.6	36.8	40.8
95-99	39.610309543532814	41.0	39.0	41.0	37.0	41.0
100-104	39.46091781243931	40.0	39.0	41.0	36.2	41.0
105-109	39.436025623497116	40.0	39.0	41.0	36.0	41.0
110-114	39.29881918989167	40.0	38.8	41.0	35.4	41.0
115-119	39.09621145275692	40.0	38.0	41.0	35.0	41.0
120-124	38.83518236226344	40.0	38.0	41.0	35.0	41.0
125-129	38.23663207434529	39.4	36.6	40.4	34.4	40.8
130-134	38.300077003901286	39.6	36.4	40.8	34.0	41.0
135-139	37.86613594049525	38.8	35.2	41.0	34.0	41.0
140-144	37.19732808897523	37.2	35.0	40.0	34.0	41.0
145-149	36.469907772440465	36.2	35.0	39.0	33.2	40.8
150-154	35.76585335411902	35.0	35.0	37.2	33.0	39.4
155-159	35.18767176777678	35.0	34.8	36.2	33.0	38.2
160-164	34.76707408400794	35.0	34.0	35.4	33.0	36.8
165-169	34.48437252159152	35.0	34.0	35.0	33.0	36.0
170-174	34.29348511952729	35.0	34.0	35.0	32.0	36.0
175-178	33.805163149190825	34.75	33.25	35.0	31.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	11.0
30	201.0
31	2461.0
32	18073.0
33	83228.0
34	264978.0
35	663175.0
36	1514816.0
37	3113645.0
38	3402352.0
39	871760.0
40	20769.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.401071109758867	28.570406878872305	28.966430411264398	21.062091600104424
2	21.556935188086065	28.95598389186888	28.956516262568844	20.53056465747621
3	20.33038322955955	28.55812217385238	29.847021772655815	21.264472823932255
4	20.67330027344769	27.893050543374702	30.118169219350687	21.315479963826917
5	21.17056464140464	28.436902369943596	29.166029244830156	21.226503743821613
6	21.010963923447505	28.58120496382441	29.20565570542181	21.202175407306274
7	20.99566579937118	28.534326208037008	29.001617101112963	21.468390891478844
8	21.20436515848726	28.649057116244347	28.790969064340416	21.355608660927977
9	21.213023715909316	28.6789803674744	28.810847585382465	21.297148331233817
10-14	21.310777021152898	28.645529407002325	28.564269548727438	21.479424023117343
15-19	21.35728010403126	28.51988590391874	28.600010707682383	21.522823284367618
20-24	21.4041930119013	28.445595079448292	28.588049442974512	21.5621624656759
25-29	21.471187344363184	28.39985539606421	28.45070182027587	21.67825543929673
30-34	21.462677448947908	28.435926022169323	28.43371216363589	21.66768436524688
35-39	21.461367616131394	28.444773420518914	28.419623425074196	21.674235538275493
40-44	21.54504423648951	28.368839278189707	28.325839797200913	21.760276688119866
45-49	21.705225539851515	28.31240798399352	28.254560382840825	21.727806093314136
50-54	21.67441634341888	28.364305086982846	28.273816130611223	21.687462438987055
55-59	21.65131949082459	28.337367129564665	28.257935412183997	21.753377967426747
60-64	21.591981251711996	28.36180394916603	28.302033786655358	21.744181012466615
65-69	21.591501113609013	28.402041129353123	28.279678235148943	21.726779521888922
70-74	21.55058691860725	28.403887350761675	28.270589763274838	21.774935967356235
75-79	21.526549879267364	28.434232480659627	28.274840693090404	21.76437694698261
80-84	21.51207944095853	28.501056052708318	28.22355230075047	21.76331220558268
85-89	21.46768374247361	28.49166021209046	28.198189357025772	21.84246668841016
90-94	20.99572676002557	28.56487139310629	29.24304334157665	21.19635850529149
95-99	21.02160433971802	28.657043230200546	28.858742303839396	21.462610126242033
100-104	21.218937246558472	28.635808908760062	28.597438371657585	21.547815473023878
105-109	21.249506450784498	28.519959052467154	28.61548483746682	21.61504965928153
110-114	21.305535067027915	28.455252244203777	28.56586595066363	21.673346738104684
115-119	21.374833995517243	28.50529838828166	28.426895976501694	21.692971639699408
120-124	21.408159867159817	28.481128028627527	28.371431124282324	21.739280979930328
125-129	21.435088686666724	28.466331857050324	28.382665190328677	21.71591426595427
130-134	21.54253616107744	28.453055376211147	28.320635333729978	21.683773128981443
135-139	21.599061817905678	28.42973961936141	28.273231776086938	21.697966786645978
140-144	21.564250470255068	28.493404119387257	28.322557436769834	21.619787973587844
145-149	21.516940947276456	28.557489734388092	28.33724948355335	21.5883198347821
150-154	21.445957994921013	28.608730194009606	28.3789679234657	21.56634388760367
155-159	21.397073163301712	28.676647726599008	28.423197673785268	21.503081436314012
160-164	21.317077680810858	28.806264668213093	28.466682122900323	21.409975528075726
165-169	21.24778545082025	28.8855670341416	28.476738892604313	21.38990862243384
170-174	21.178214572171626	29.013067290160837	28.53590671455256	21.27281142311497
175-178	21.082786146756202	29.12307143843964	28.57637036427083	21.217772050533327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	2.0
11	7.5
12	24.0
13	78.0
14	239.0
15	575.5
16	1168.5
17	2234.0
18	4043.0
19	7013.5
20	11508.5
21	17798.5
22	26241.0
23	36627.5
24	49274.5
25	63443.5
26	78820.0
27	96098.5
28	114687.5
29	134627.5
30	155483.0
31	177077.5
32	199816.5
33	224339.5
34	249907.5
35	276910.0
36	305285.5
37	334285.5
38	362509.0
39	389143.0
40	413040.0
41	432416.5
42	446868.0
43	454229.0
44	453223.0
45	444631.0
46	428448.5
47	405578.0
48	378616.0
49	348185.0
50	315520.5
51	282167.5
52	249884.0
53	219716.0
54	191508.5
55	166413.5
56	144639.5
57	125777.0
58	109323.5
59	95453.0
60	83802.5
61	73442.0
62	64619.5
63	56465.5
64	48948.0
65	41978.0
66	35250.0
67	29333.5
68	24154.0
69	19320.0
70	15163.5
71	11687.5
72	8661.5
73	6167.5
74	4343.0
75	3029.0
76	1999.0
77	1285.0
78	799.0
79	454.0
80	268.0
81	154.5
82	79.5
83	38.5
84	18.5
85	7.0
86	2.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1319.0
92-93	2922.0
94-95	4340.0
96-97	5945.0
98-99	7383.0
100-101	8902.0
102-103	10371.0
104-105	11602.0
106-107	12654.0
108-109	14572.0
110-111	16036.0
112-113	17009.0
114-115	18017.0
116-117	19543.0
118-119	20833.0
120-121	22028.0
122-123	23424.0
124-125	24791.0
126-127	26362.0
128-129	27919.0
130-131	29502.0
132-133	32995.0
134-135	109207.0
136-137	107553.0
138-139	105828.0
140-141	109415.0
142-143	114113.0
144-145	113275.0
146-147	109027.0
148-149	111398.0
150-151	117920.0
152-153	121773.0
154-155	117253.0
156-157	121354.0
158-159	127197.0
160-161	131940.0
162-163	138711.0
164-165	143875.0
166-167	149111.0
168-169	157392.0
170-171	162982.0
172-173	167738.0
174-175	179658.0
176-177	561220.0
178-179	6319060.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.68853060144745
#Duplication Level	Percentage of total
1	85.6931106179499
2	9.503140452852529
3	2.207982077954883
4	1.0583597886277425
5	0.584822500804653
6	0.3829653059200288
7	0.1955299899874208
8	0.10652836197872675
9	0.07587792257021558
>10	0.1692912575552047
>50	0.006984404126461647
>100	0.0036717445406472507
>500	0.011735575131667458
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.0089460376000367E-5	0.0	0.0
2	1.0044730188000183E-5	0.0	0.0	4.0178920752000733E-5	0.0	0.0
3	1.0044730188000183E-5	0.0	1.0044730188000183E-5	5.022365094000092E-5	1.0044730188000183E-5	0.0
4	1.0044730188000183E-5	3.013419056400055E-5	1.0044730188000183E-5	6.02683811280011E-5	1.0044730188000183E-5	0.0
5	1.0044730188000183E-5	4.0178920752000733E-5	1.0044730188000183E-5	6.02683811280011E-5	1.0044730188000183E-5	0.0
6	1.0044730188000183E-5	4.0178920752000733E-5	1.0044730188000183E-5	6.02683811280011E-5	1.0044730188000183E-5	0.0
7	1.0044730188000183E-5	4.0178920752000733E-5	1.0044730188000183E-5	7.031311131600129E-5	1.0044730188000183E-5	0.0
8	1.0044730188000183E-5	4.0178920752000733E-5	2.0089460376000367E-5	9.040257169200165E-5	3.013419056400055E-5	0.0
9	2.0089460376000367E-5	4.0178920752000733E-5	2.0089460376000367E-5	9.040257169200165E-5	3.013419056400055E-5	0.0
10-14	2.410735245120044E-5	4.0178920752000733E-5	2.8125244526400514E-5	1.0848308603040198E-4	3.0134190564000547E-5	0.0
15-19	4.4196812827200805E-5	5.223259697760095E-5	4.0178920752000733E-5	1.4464411470720265E-4	4.0178920752000733E-5	0.0
20-24	7.031311131600129E-5	8.035784150400147E-5	4.620575886480085E-5	1.8482303545920337E-4	4.218786678960077E-5	0.0
25-29	8.035784150400147E-5	1.1049203206800202E-4	6.026838112800109E-5	2.18975118098404E-4	5.424154301520099E-5	0.0
30-34	1.3459938451920245E-4	1.366083305568025E-4	6.026838112800109E-5	2.471003626248045E-4	9.241151772960168E-5	2.008946037600037E-6
35-39	4.0379815355760736E-4	1.4062622263200257E-4	6.026838112800109E-5	2.9531506752720533E-4	1.366083305568025E-4	3.816997471440069E-5
40-44	6.167464335432111E-4	1.466530607448027E-4	7.232205735360131E-5	3.6562817884320663E-4	2.350466863992043E-4	5.022365094000093E-5
45-49	7.533547641000138E-4	1.64733575083203E-4	8.035784150400147E-5	3.9576236940720727E-4	3.354939882792061E-4	6.830416527840125E-5
50-54	9.000078248448164E-4	1.7076041319600313E-4	9.040257169200165E-5	4.560307505352084E-4	4.4397707430960813E-4	8.638467961680156E-5
55-59	0.0010265714252136186	1.7879619734640324E-4	1.0446519395520193E-4	5.002275633624092E-4	5.263438618512097E-4	9.642940980480175E-5
60-64	0.0011832692161464215	2.008946037600037E-4	1.1049203206800202E-4	5.323706999640097E-4	6.227732716560113E-4	1.1651887018080214E-4
65-69	0.0012676449497256233	2.1093933394800385E-4	1.3058149244400238E-4	5.805854048664107E-4	7.171937354232131E-4	1.4062622263200257E-4
70-74	0.0024227889213456442	2.1093933394800385E-4	1.6272462904560296E-4	6.10719595430411E-4	8.136231452280148E-4	1.4062622263200257E-4
75-79	0.026256924711432483	2.2098406413600404E-4	1.8884092753440344E-4	6.308090558064114E-4	9.140704471080167E-4	1.4062622263200257E-4
80-84	0.03738447681369909	2.2701090224880416E-4	2.129482799856039E-4	6.549164082576119E-4	0.0011330455652064208	1.4062622263200257E-4
85-89	0.03747889727746629	2.691987690384049E-4	2.2701090224880416E-4	6.890684908968126E-4	0.001524790042538428	1.4062622263200257E-4
90-94	0.037549210388782284	2.8527033733920525E-4	2.3102879432400423E-4	7.151847893856131E-4	0.0015709958014032289	1.4062622263200257E-4
95-99	0.03758938930953429	3.073687437528056E-4	2.491093086624045E-4	7.533547641000138E-4	0.001589076315741629	1.4062622263200257E-4
100-104	0.037639612960474286	3.2344031205360586E-4	2.631719309256048E-4	7.875068467392144E-4	0.0016051478840424294	1.4062622263200257E-4
105-109	0.037691845557451885	3.616102867680066E-4	2.872792833768053E-4	8.337126056040152E-4	0.0016172015602680296	1.4062622263200257E-4
110-114	0.03772800658612869	4.037981535576074E-4	2.9531506752720544E-4	8.598289040928157E-4	0.0016292552364936298	1.4062622263200257E-4
115-119	0.03777019445291829	4.3393234412160794E-4	3.0134190564000553E-4	8.939809867320164E-4	0.00165135364290723	1.4062622263200257E-4
120-124	0.03795300854233989	4.560307505352084E-4	3.053597977152055E-4	9.100525550328166E-4	0.0016875146715840308	1.4062622263200257E-4
125-129	0.0382563593940175	4.90182833174409E-4	3.113866358280057E-4	9.442046376720171E-4	0.0017457741066744317	1.4062622263200257E-4
130-134	0.038736497497003905	5.203170237384095E-4	3.194224199784058E-4	9.663030440856177E-4	0.001812069325915233	1.4062622263200257E-4
135-139	0.03926685925093032	5.363885920392098E-4	3.3147609620400606E-4	0.0010064819648376183	0.001860284030817634	1.4062622263200257E-4
140-144	0.03979521205881913	5.685317286408104E-4	3.455387184672063E-4	0.0010325982633264189	0.0019265792500584352	1.4263516866960262E-4
145-149	0.040070437665970335	5.745585667536106E-4	3.5357450261760644E-4	0.0010506787776648192	0.0019808207930736366	1.5669779093280283E-4
150-154	0.04028941278406874	6.04692757317611E-4	3.6562817884320663E-4	0.00109286664445442	0.0020511339043896375	1.6875146715840308E-4
155-159	0.04060883520404714	6.127285414680112E-4	3.776818550688069E-4	0.0011149650508680204	0.0020993486092920383	1.7276935923360315E-4
160-164	0.04100459757345435	6.207643256184112E-4	3.897355312944071E-4	0.0011511260795448212	0.0021696617206080396	1.808051433840033E-4
165-167	0.04132067175003676	6.227732716560113E-4	3.9174447733200717E-4	0.0011718851886000213	0.0022131888847560406	1.808051433840033E-4
>>END_MODULE
