##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479369
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9590788
Total Bases	1.6 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.325487645019365	39.0	39.0	39.0	37.0	39.0
2	40.035026319005276	41.0	40.0	41.0	38.0	41.0
3	40.04121496586099	41.0	40.0	41.0	38.0	41.0
4	40.036204011599466	41.0	40.0	41.0	38.0	41.0
5	40.03283150456459	41.0	40.0	41.0	38.0	41.0
6	40.01168996749798	41.0	40.0	41.0	38.0	41.0
7	40.026548392061216	41.0	40.0	41.0	38.0	41.0
8	39.99149704904331	41.0	40.0	41.0	38.0	41.0
9	39.965503668728786	41.0	40.0	41.0	38.0	41.0
10-14	39.85498424112805	40.8	40.0	41.0	38.0	41.0
15-19	39.796478996303534	40.6	39.8	41.0	37.8	41.0
20-24	39.828483248717404	41.0	40.0	41.0	38.0	41.0
25-29	39.67642085301021	40.4	39.6	41.0	37.2	41.0
30-34	39.438416593089116	40.0	39.0	41.0	36.4	41.0
35-39	39.09867182967656	40.0	38.0	41.0	35.2	41.0
40-44	38.671994501390294	40.0	37.4	41.0	35.0	41.0
45-49	38.249541476675326	39.4	36.4	41.0	34.6	41.0
50-54	37.57181370289908	38.2	35.0	40.2	34.0	41.0
55-59	36.79337372487015	36.6	35.0	39.4	33.4	41.0
60-64	35.76249542790436	35.4	34.6	38.0	32.4	40.0
65-69	35.353384768801064	35.0	34.2	37.0	32.8	39.0
70-74	35.19546648304602	35.0	34.0	36.0	33.0	39.0
75-79	34.96931282393063	35.0	34.0	35.8	32.6	38.4
80-84	34.88550899050214	35.0	34.0	35.0	32.0	38.8
85-89	34.615357653615106	34.8	33.4	35.2	31.0	39.4
90-94	39.48130251061268	40.6	39.8	40.6	37.0	41.0
95-99	39.65385296395431	41.0	39.4	41.0	37.0	41.0
100-104	39.479318649824556	40.0	39.0	41.0	36.4	41.0
105-109	39.48951763169765	41.0	39.0	41.0	36.0	41.0
110-114	39.354245863988965	40.0	39.0	41.0	35.6	41.0
115-119	39.14719645988114	40.0	38.0	41.0	35.0	41.0
120-124	38.8823188172074	40.0	38.0	41.0	35.0	41.0
125-129	38.269113979391946	39.4	36.8	40.6	34.4	40.8
130-134	38.353500929943365	39.6	36.4	40.8	34.4	41.0
135-139	37.915326048733206	39.0	35.2	41.0	34.0	41.0
140-144	37.26130315989518	37.2	35.0	40.2	34.0	41.0
145-149	36.54567723593545	36.2	35.0	39.0	33.8	41.0
150-154	35.84522219951502	35.0	35.0	37.2	33.0	39.6
155-159	35.27921593795311	35.0	35.0	36.2	33.0	38.4
160-164	34.84292351941004	35.0	34.4	35.4	33.0	36.8
165-169	34.549008467684835	35.0	34.0	35.0	33.0	36.0
170-174	34.35707381822249	35.0	34.0	35.0	32.8	36.0
175-178	33.74666494274008	34.75	33.25	35.0	30.5	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	112.0
31	1913.0
32	13982.0
33	68154.0
34	227439.0
35	596889.0
36	1461584.0
37	3120364.0
38	3294774.0
39	789543.0
40	16031.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.49414625784659	28.503038540733044	28.87034933938692	21.132465862033442
2	21.580635501483297	28.940614681504794	28.92309787266698	20.555651944344927
3	20.338182847957853	28.53722759798256	29.79996012840655	21.324629425653033
4	20.68639198364097	27.869722487870654	30.0858594726523	21.35802605583608
5	21.221499213620405	28.426694448881573	29.065192557691816	21.286613779806203
6	21.060813772549242	28.52349567105435	29.17071047759579	21.244980078800616
7	21.019430311669907	28.456566863953203	28.967640615140276	21.556362209236614
8	21.2351477271732	28.590351491452004	28.802336158405335	21.372164622969457
9	21.25546931075945	28.611246541994255	28.77542491816105	21.357859229085243
10-14	21.342867760188213	28.5921156843421	28.540703850403116	21.52431270506657
15-19	21.398308460159896	28.46772548824977	28.55885877156288	21.57510728002746
20-24	21.432562162775362	28.38240194653453	28.567984194833624	21.617051695856482
25-29	21.49722212606514	28.357173571139306	28.42315146576069	21.722452837034872
30-34	21.495447506503115	28.39757066885432	28.404819291178157	21.702162533464403
35-39	21.49213391016463	28.395191302320516	28.3996664299117	21.71300835760315
40-44	21.57697782497121	28.33272719613863	28.29216952767593	21.79812545121423
45-49	21.739394093582302	28.28422648900174	28.221092990482116	21.755286426933846
50-54	21.70970101726782	28.33142386214772	28.232262041450607	21.726613079133852
55-59	21.672569553200425	28.29046998015179	28.230011965648703	21.806948500999084
60-64	21.610578817924033	28.322146209466837	28.271887565443006	21.795387407166125
65-69	21.601353298602785	28.34150645390139	28.280789857934508	21.776350389561316
70-74	21.555146459289894	28.3659340608926	28.24821067883056	21.830708800986947
75-79	21.511861173451024	28.386583041977364	28.27995572418033	21.821600060391283
80-84	21.491376933782707	28.43699391541133	28.250873650840784	21.820755499965173
85-89	21.433813363406635	28.395082864932476	28.24744953177987	21.923654239881017
90-94	21.058678004624817	28.50189936494286	29.182567705807084	21.256854924625237
95-99	21.096781816659767	28.60307123709367	28.783834535060947	21.516312411185616
100-104	21.277931837204996	28.58964495614566	28.531512427448842	21.6009107792005
105-109	21.322016481402063	28.448405703794037	28.565224640105026	21.664353174698878
110-114	21.387042994425027	28.387257477232964	28.495825058147073	21.72987447019494
115-119	21.450526597450782	28.421242260967635	28.394884154397975	21.73334698718361
120-124	21.464914384948084	28.428851995690295	28.333868193236018	21.772365426125603
125-129	21.4996640563409	28.406826272378577	28.34649344620812	21.7470162250724
130-134	21.58382536434496	28.401175811151084	28.283384481565882	21.731614342938073
135-139	21.631811153477983	28.39719244706389	28.24034951843234	21.730646881025788
140-144	21.59744997770862	28.434160717523028	28.300247079677593	21.668142225090758
145-149	21.55598876432008	28.512474804864457	28.310259483492946	21.621276947322514
150-154	21.48860607549666	28.558685469979046	28.361694024801732	21.591014429722566
155-159	21.428940392282936	28.648778152622096	28.399708983820602	21.522572471274362
160-164	21.367442936630727	28.773456422710392	28.437553020846995	21.421547619811886
165-169	21.279667101525504	28.845979400124065	28.48721534859175	21.387138149758684
170-174	21.200459792068894	28.993249120485036	28.54045704418389	21.26583404326218
175-178	21.141809202850297	29.118826903844585	28.553239479231213	21.18612441407391
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	1.5
11	9.0
12	23.0
13	74.0
14	216.0
15	502.0
16	1104.0
17	2082.0
18	3740.5
19	6529.5
20	10626.0
21	16664.0
22	24817.0
23	34668.0
24	46248.0
25	59743.0
26	74700.5
27	90645.0
28	108291.5
29	127609.0
30	147685.0
31	168751.0
32	190842.0
33	214053.0
34	238596.5
35	264919.0
36	292151.0
37	319510.5
38	347680.5
39	374217.0
40	397017.0
41	416607.5
42	430585.5
43	437132.0
44	436519.5
45	428872.5
46	414260.0
47	393488.5
48	367665.0
49	337622.0
50	305631.5
51	273945.0
52	242834.0
53	213546.0
54	186677.0
55	162196.5
56	141175.5
57	122694.5
58	106707.5
59	93116.5
60	81535.5
61	71969.5
62	63021.0
63	54779.0
64	47463.5
65	40549.0
66	34200.0
67	28575.5
68	23470.0
69	18665.0
70	14635.5
71	11166.5
72	8328.0
73	6049.0
74	4262.0
75	2975.5
76	1996.5
77	1246.0
78	734.0
79	438.5
80	256.5
81	136.5
82	70.5
83	33.0
84	15.5
85	5.5
86	2.0
87	2.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1122.0
92-93	2521.0
94-95	3979.0
96-97	5278.0
98-99	6505.0
100-101	8097.0
102-103	9342.0
104-105	10688.0
106-107	12373.0
108-109	14127.0
110-111	15408.0
112-113	16801.0
114-115	17997.0
116-117	19340.0
118-119	20772.0
120-121	22360.0
122-123	23862.0
124-125	25673.0
126-127	27013.0
128-129	28656.0
130-131	31103.0
132-133	35838.0
134-135	98001.0
136-137	100971.0
138-139	100166.0
140-141	102473.0
142-143	107360.0
144-145	108545.0
146-147	109099.0
148-149	115334.0
150-151	123884.0
152-153	125145.0
154-155	118148.0
156-157	122970.0
158-159	129129.0
160-161	136276.0
162-163	144328.0
164-165	150961.0
166-167	159532.0
168-169	168393.0
170-171	177333.0
172-173	186647.0
174-175	208998.0
176-177	1032974.0
178-179	5405266.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.97218771373362
#Duplication Level	Percentage of total
1	86.09558515438941
2	9.382847462735143
3	2.177631052853579
4	0.9464135737957994
5	0.5720376887188872
6	0.306472453301696
7	0.1710341480474377
8	0.10563927362318341
9	0.06142197123610501
>10	0.16132116312366362
>50	0.004217049240175137
>100	0.0035489079085828604
>500	0.011830101026387544
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.042667192727021E-5	1.042667192727021E-5	1.042667192727021E-5	2.085334385454042E-5	0.0	0.0
2	3.128001578181063E-5	1.042667192727021E-5	1.042667192727021E-5	2.085334385454042E-5	0.0	0.0
3	3.128001578181063E-5	2.085334385454042E-5	1.042667192727021E-5	2.085334385454042E-5	0.0	0.0
4	3.128001578181063E-5	2.085334385454042E-5	2.085334385454042E-5	2.085334385454042E-5	1.042667192727021E-5	0.0
5	4.170668770908084E-5	2.085334385454042E-5	2.085334385454042E-5	3.128001578181063E-5	1.042667192727021E-5	0.0
6	4.170668770908084E-5	2.085334385454042E-5	2.085334385454042E-5	4.170668770908084E-5	2.085334385454042E-5	0.0
7	5.213335963635105E-5	3.128001578181063E-5	2.085334385454042E-5	4.170668770908084E-5	3.128001578181063E-5	0.0
8	5.213335963635105E-5	5.213335963635105E-5	2.085334385454042E-5	5.213335963635105E-5	5.213335963635105E-5	0.0
9	5.213335963635105E-5	5.213335963635105E-5	2.085334385454042E-5	5.213335963635105E-5	5.213335963635105E-5	0.0
10-14	6.46453659490753E-5	5.630402840725913E-5	2.9194681396356587E-5	8.549870980361572E-5	7.507203787634551E-5	0.0
15-19	8.132804103270763E-5	7.090136910543744E-5	6.88160347199834E-5	1.4597340698178294E-4	1.1469339119997233E-4	0.0
20-24	9.384004734543189E-5	9.384004734543189E-5	1.0009605050179402E-4	1.9185076346177186E-4	1.4597340698178294E-4	0.0
25-29	1.0426671927270212E-4	1.1052272242906421E-4	1.1260805681451826E-4	2.231307792435825E-4	1.6682675083632336E-4	0.0
30-34	1.7933875714904764E-4	1.292907318981506E-4	1.2512006312724252E-4	2.4606945748357697E-4	2.523254606399391E-4	2.085334385454042E-6
35-39	3.6076284868354926E-4	1.4597340698178294E-4	1.4597340698178294E-4	2.710934701090254E-4	3.232268297453765E-4	1.8768009469086377E-5
40-44	5.505282777598672E-4	1.5640007890905315E-4	1.689120852217774E-4	3.2531216413083053E-4	4.0246953639263005E-4	2.9194681396356587E-5
45-49	7.131843598252823E-4	1.9810676661813397E-4	1.939360978472259E-4	3.8578686130899775E-4	4.7128557111261347E-4	3.3365350167264674E-5
50-54	8.362190885670707E-4	2.1478944170176634E-4	2.1687477608722038E-4	4.1081087393444625E-4	5.48442943374413E-4	4.170668770908084E-5
55-59	9.613391516943133E-4	2.273014480144906E-4	2.2938678239994463E-4	4.46261558487165E-4	6.172589780943964E-4	4.587735647998893E-5
60-64	0.0010635205365815612	2.5024012625448505E-4	2.6066679818175523E-4	5.046509212798782E-4	7.298670349089147E-4	5.630402840725913E-5
65-69	0.0011823845965524419	2.7526413887993354E-4	2.940321483490199E-4	5.463576089889589E-4	8.195364134834385E-4	6.256003156362126E-5
70-74	0.0024231585558975965	2.856908108072037E-4	3.169708265890143E-4	5.567842809162291E-4	8.9252311697433E-4	6.256003156362126E-5
75-79	0.026527538717360865	3.086294890471982E-4	3.378241704435548E-4	5.94320299854402E-4	9.446564766106811E-4	6.673070033452935E-5
80-84	0.03743383755328551	3.378241704435548E-4	3.524215111417331E-4	6.44368325105299E-4	0.0010593498678106532	7.298670349089147E-5
85-89	0.03749222691607822	3.4616550798537095E-4	3.7118952061081955E-4	6.860750128143797E-4	0.001292907318981506	7.298670349089147E-5
90-94	0.03751099492554731	3.6910418622536546E-4	3.837015269235437E-4	7.048430222834662E-4	0.001332528672305133	7.298670349089147E-5
95-99	0.037527677600630936	3.7953085815263564E-4	4.0246953639263005E-4	7.361230380652768E-4	0.0013429553442324028	7.298670349089147E-5
100-104	0.037556872282027295	3.98298867621722E-4	4.2540821463262456E-4	7.799150601598117E-4	0.0013554673505451272	7.298670349089147E-5
105-109	0.037596493635350926	4.0664020516353817E-4	4.379202209453488E-4	7.945124008579901E-4	0.0013638086880869433	7.298670349089147E-5
110-114	0.037663224335685454	4.0664020516353817E-4	4.5877356479988925E-4	8.257924166398007E-4	0.001390918035097846	7.298670349089147E-5
115-119	0.037721613698478165	4.149815427053543E-4	4.692002367271594E-4	8.549870980361571E-4	0.0014201127164942026	7.298670349089147E-5
120-124	0.03795725648403447	4.4000555533080287E-4	4.775415742689756E-4	8.946084513597839E-4	0.001484758082443278	7.298670349089147E-5
125-129	0.03833887267657256	4.6502956795625136E-4	4.8796824619624576E-4	9.488271453815891E-4	0.001593195470486888	7.298670349089147E-5
130-134	0.0386912941877143	4.921389149671539E-4	4.963095837380619E-4	0.0010009605050179402	0.0016349021581959686	7.298670349089147E-5
135-139	0.03908542238656511	5.213335963635105E-4	5.150775932071483E-4	0.0010322405207997508	0.0016515848332796013	7.298670349089147E-5
140-144	0.03945035590401956	5.484429433744131E-4	5.317602682907807E-4	0.0010676912053524696	0.0017058035273014062	7.298670349089147E-5
145-149	0.03971936403974313	5.838936279271318E-4	5.421869402180509E-4	0.00109897122113428	0.001739168877468671	7.298670349089147E-5
150-154	0.03993206814705945	5.880642966980397E-4	5.651256184580454E-4	0.0011323365713015448	0.0018038142434177464	7.298670349089147E-5
155-159	0.04020733228593938	6.130883093234884E-4	6.005763030107641E-4	0.00119698193725062	0.0018517769342831895	7.298670349089147E-5
160-164	0.04059311914724838	6.339416531780288E-4	6.047469717816721E-4	0.0012678833063560573	0.0019101662970759023	7.298670349089147E-5
165-167	0.04101157624726282	6.429781021816629E-4	6.116980863998523E-4	0.0013033339909087763	0.0019497876503995293	7.298670349089147E-5
>>END_MODULE
