##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479371
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7244382
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.169152593002416	39.0	39.0	39.0	37.0	39.0
2	39.91958320254233	41.0	40.0	41.0	38.0	41.0
3	39.984338346597404	41.0	40.0	41.0	38.0	41.0
4	40.00110181931323	41.0	40.0	41.0	38.0	41.0
5	40.012509417642526	41.0	40.0	41.0	38.0	41.0
6	39.99977582628856	41.0	40.0	41.0	38.0	41.0
7	40.01686631102557	41.0	40.0	41.0	38.0	41.0
8	39.99612679176774	41.0	40.0	41.0	38.0	41.0
9	39.972928677698114	41.0	40.0	41.0	38.0	41.0
10-14	39.902696185816815	41.0	40.0	41.0	38.0	41.0
15-19	39.7569930740814	41.0	39.8	41.0	37.6	41.0
20-24	39.54456877066947	40.0	39.0	41.0	36.8	41.0
25-29	39.277389430872084	40.0	38.4	41.0	35.8	41.0
30-34	39.17750955153939	40.0	38.0	41.0	35.0	41.0
35-39	39.032633563497896	40.0	37.8	41.0	35.0	41.0
40-44	38.50670585841553	39.8	36.6	41.0	35.0	41.0
45-49	37.86925410614735	38.8	35.0	40.8	34.4	41.0
50-54	37.1759521516121	37.2	35.0	39.8	34.0	41.0
55-59	36.489452682092136	35.8	35.0	39.0	33.8	40.8
60-64	35.77799138698097	35.0	34.8	37.0	32.8	39.6
65-69	35.41778580974885	35.0	34.8	36.2	33.0	39.0
70-74	35.1852576796751	35.0	35.0	36.0	33.0	37.4
75-79	35.02045676221933	35.0	35.0	35.0	33.0	37.0
80-84	34.94954998231733	35.0	35.0	35.0	33.0	37.0
85-89	34.78976721547815	35.0	34.0	35.0	32.4	37.6
90-94	39.28805648901874	40.6	38.8	40.6	36.4	40.6
95-99	39.535330834327205	41.0	39.0	41.0	36.0	41.0
100-104	39.402251163035494	40.4	39.0	41.0	36.0	41.0
105-109	39.22347290002593	40.0	38.6	41.0	35.2	41.0
110-114	38.999451297908266	40.0	38.0	41.0	35.0	41.0
115-119	38.70559307548754	40.0	37.8	41.0	34.8	41.0
120-124	38.37121011009985	40.0	37.0	41.0	34.0	41.0
125-129	37.90296390949057	39.2	35.8	40.6	33.6	40.8
130-134	37.65218393883522	38.8	35.0	40.6	33.2	41.0
135-139	37.32079335335126	37.8	35.0	40.0	33.6	41.0
140-144	36.86866243354963	36.6	35.0	39.6	33.6	41.0
145-149	36.19501772324239	35.4	35.0	38.4	33.0	40.6
150-154	35.56991620503349	35.0	35.0	36.8	33.0	39.2
155-159	35.0689421308836	35.0	35.0	36.0	33.0	37.4
160-164	34.71990051171192	35.0	34.6	35.0	33.0	36.4
165-169	34.503697238541065	35.0	34.0	35.0	33.0	36.0
170-174	34.38271310009392	35.0	34.0	35.0	33.0	35.6
175-178	34.08747386207507	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	46.0
30	569.0
31	4120.0
32	20992.0
33	77363.0
34	227820.0
35	582751.0
36	1360612.0
37	2293303.0
38	2200367.0
39	472881.0
40	3556.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.10909087897353	27.259923620814032	27.49382901122552	23.137156488986914
2	22.749366336562595	27.77597868251564	27.80466297884347	21.6699920020783
3	21.547303827986983	27.045881898552558	28.45400753300972	22.952806740450736
4	22.035737485958084	26.47483249778932	28.655377366903075	22.834052649349523
5	22.56614849962357	27.085181317053685	27.63000349788291	22.718666685439835
6	22.668903986565038	27.029124085394724	27.435770228571606	22.866201699468636
7	22.589545940564705	27.256127575823584	27.325947196047917	22.828379287563795
8	22.666626359570767	27.40519481164853	27.300631026911613	22.62754780186909
9	22.841382467131083	27.34274642060565	27.084284070055944	22.731587042207327
10-14	22.706439279430597	27.31357346975905	27.20780599366516	22.772181257145192
15-19	22.736277573435526	27.260003682853828	27.20113599752194	22.8025827461887
20-24	22.71039820926064	27.204297067713988	27.2497612632796	22.83554345974577
25-29	22.714047933971454	27.20805722282453	27.138751103958903	22.939143739245115
30-34	22.771598736786657	27.230808645927283	27.126780448629024	22.870812168657036
35-39	22.746793308249067	27.217043496601917	27.196520006813557	22.839643188335458
40-44	22.75298845367348	27.254493205907693	27.153996020640548	22.838522319778278
45-49	22.906638551086896	27.136017951565776	27.060599510075534	22.89674398727179
50-54	22.867308764225854	27.22851445437306	27.08274632673981	22.821430454661282
55-59	22.813015658202453	27.19351353918112	27.089620619122513	22.903850183493912
60-64	22.81013894628969	27.2404216122231	27.081774539222252	22.867664902264956
65-69	22.786341195149564	27.20953146866082	27.14951806793181	22.854609268257803
70-74	22.729624141852263	27.27182801790408	27.11942302324753	22.87912481699612
75-79	22.734041357841154	27.234044256639145	27.13943577243718	22.892478613082524
80-84	22.70245550276062	27.293077587570618	27.109696865792003	22.894770043876758
85-89	22.708098496186423	27.264329793762947	27.0817966253022	22.94577508474843
90-94	22.085041575090145	27.224887344618608	27.919416838898048	22.770654241393196
95-99	22.462861675279733	27.401058787538084	27.289257292130177	22.84682224505201
100-104	22.50625581303862	27.41407736679126	27.240241712068286	22.83942510810183
105-109	22.467233046613654	27.416065403726762	27.279820159616435	22.836881390043146
110-114	22.533594993713297	27.276261117859697	27.254639311317515	22.935504577109494
115-119	22.532576872854026	27.32458209153877	27.181558383979365	22.961282651627837
120-124	22.465021971639477	27.36972878086419	27.236576497613985	22.928672749882352
125-129	22.488640863190472	27.33613788693619	27.223502467828936	22.9517187820444
130-134	22.619196013722227	27.35547194950396	27.143489746895423	22.88184228987839
135-139	22.713239917636844	27.31654039255062	27.10073079009337	22.869488899719165
140-144	22.682247867162648	27.358046501923656	27.17687346111335	22.782832169800347
145-149	22.62700587766711	27.401285856969753	27.21626012397056	22.75544814139258
150-154	22.526086790973547	27.529743374080866	27.258940754826718	22.68522908011887
155-159	22.47054849902952	27.532232378356536	27.334447795915413	22.66277132669853
160-164	22.399759002071146	27.670943368181494	27.37590457548581	22.55339305426155
165-169	22.2987060976015	27.728673963408262	27.462554185815307	22.51006575317493
170-174	22.22900934382372	27.84590094290988	27.50547504812228	22.41961466514412
175-178	22.147644678700708	27.95430096658379	27.544596768065034	22.353457586650467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	2.0
11	6.0
12	20.0
13	33.5
14	52.5
15	116.0
16	235.5
17	462.0
18	907.0
19	1653.5
20	2998.5
21	5118.0
22	8303.5
23	12717.0
24	18360.0
25	25686.5
26	34831.5
27	45346.0
28	57234.0
29	70454.0
30	85033.5
31	100678.0
32	117282.5
33	135059.0
34	154293.5
35	173861.0
36	193394.5
37	213728.5
38	233337.5
39	252284.0
40	269548.5
41	283702.5
42	294884.5
43	301245.5
44	302558.5
45	299432.0
46	291604.0
47	280432.0
48	266300.0
49	249705.5
50	232499.5
51	215475.0
52	198416.0
53	182597.0
54	168335.0
55	155487.0
56	144234.0
57	133768.5
58	124568.5
59	116370.5
60	108298.0
61	100848.0
62	93393.5
63	84939.5
64	76190.5
65	67245.5
66	57826.5
67	48378.0
68	39775.0
69	31650.5
70	24195.0
71	18202.5
72	13322.0
73	9364.0
74	6304.5
75	4145.5
76	2681.5
77	1665.0
78	1002.5
79	582.0
80	330.0
81	184.0
82	100.0
83	49.0
84	25.5
85	10.0
86	5.5
87	3.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1499.0
92-93	3075.0
94-95	4486.0
96-97	5413.0
98-99	6651.0
100-101	9073.0
102-103	8990.0
104-105	10834.0
106-107	11385.0
108-109	13151.0
110-111	13847.0
112-113	14801.0
114-115	16538.0
116-117	16307.0
118-119	16862.0
120-121	17885.0
122-123	18900.0
124-125	20274.0
126-127	19755.0
128-129	21940.0
130-131	22435.0
132-133	24287.0
134-135	117368.0
136-137	93553.0
138-139	85375.0
140-141	84349.0
142-143	94859.0
144-145	94911.0
146-147	90248.0
148-149	96364.0
150-151	91992.0
152-153	86059.0
154-155	92023.0
156-157	92402.0
158-159	96608.0
160-161	89840.0
162-163	88498.0
164-165	91731.0
166-167	95455.0
168-169	102054.0
170-171	101189.0
172-173	105525.0
174-175	113049.0
176-177	247851.0
178-179	4694691.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.6832801254238
#Duplication Level	Percentage of total
1	92.03914876530561
2	6.49645167769178
3	0.8479621906440353
4	0.25502903592679876
5	0.11196123887806822
6	0.05998653205833026
7	0.03644780326617763
8	0.023067287885816863
9	0.02216953993320795
>10	0.09898839671649634
>50	0.008787531693675394
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.760759993053928E-5	0.0	0.0	1.380379996526964E-5	0.0	0.0
2	0.00153222179614493	0.0	2.760759993053928E-5	2.760759993053928E-5	1.380379996526964E-5	0.0
3	0.00153222179614493	4.141139989580892E-5	5.521519986107856E-5	2.760759993053928E-5	1.380379996526964E-5	0.0
4	0.00153222179614493	5.521519986107856E-5	5.521519986107856E-5	5.521519986107856E-5	1.380379996526964E-5	0.0
5	0.00153222179614493	5.521519986107856E-5	5.521519986107856E-5	5.521519986107856E-5	1.380379996526964E-5	0.0
6	0.00153222179614493	6.90189998263482E-5	6.90189998263482E-5	9.662659975688747E-5	1.380379996526964E-5	0.0
7	0.00153222179614493	6.90189998263482E-5	6.90189998263482E-5	9.662659975688747E-5	1.380379996526964E-5	0.0
8	0.00153222179614493	6.90189998263482E-5	6.90189998263482E-5	1.2423419968742675E-4	1.380379996526964E-5	0.0
9	0.0015460255961101995	6.90189998263482E-5	6.90189998263482E-5	1.2423419968742675E-4	1.380379996526964E-5	0.0
10-14	0.0015460255961101995	6.90189998263482E-5	6.90189998263482E-5	1.3527723965964247E-4	1.380379996526964E-5	0.0
15-19	0.001562590156068523	8.558355978467177E-5	8.558355978467177E-5	1.380379996526964E-4	2.2086079944431424E-5	0.0
20-24	0.0015929585159921162	1.2147343969437282E-4	1.1319115971521105E-4	1.5184179961796602E-4	3.865063990275499E-5	0.0
25-29	0.0016150445959365479	1.380379996526964E-4	1.297557196735346E-4	1.601240795971278E-4	4.141139989580892E-5	0.0
30-34	0.0016951066357351117	1.6564559958323567E-4	1.380379996526964E-4	1.9325319951377497E-4	4.96936798749707E-5	0.0
35-39	0.001874556035283617	1.9601395950682884E-4	1.4079875964575033E-4	2.374253594026378E-4	1.0214811974299533E-4	0.0
40-44	0.002031919354887691	2.0705699947904456E-4	1.601240795971278E-4	2.484683993748535E-4	1.1319115971521105E-4	0.0
45-49	0.0022362155943736815	2.2086079944431424E-4	1.7392787956239746E-4	2.484683993748535E-4	1.380379996526964E-4	1.9325319951377498E-5
50-54	0.0024377510738666183	2.733152393123389E-4	1.7944939954850532E-4	2.7055447931928494E-4	2.0153547949293676E-4	2.760759993053928E-5
55-59	0.002564746033547099	3.009228392428781E-4	2.2362155943736814E-4	2.760759993053928E-4	2.595114393470692E-4	3.8650639902754995E-5
60-64	0.0026586118733109326	3.0920511922203985E-4	2.760759993053928E-4	2.898797992706624E-4	3.009228392428781E-4	5.521519986107856E-5
65-69	0.002757999233060874	3.340519591595253E-4	2.898797992706624E-4	3.202481591942556E-4	3.368127191525792E-4	5.521519986107856E-5
70-74	0.0035475765910742973	3.671810790761724E-4	3.009228392428781E-4	3.6442031908311845E-4	3.83745639034496E-4	5.797595985413249E-5
75-79	0.017561194315816035	3.947886790067117E-4	3.0920511922203985E-4	3.947886790067116E-4	4.8037223879138344E-4	6.90189998263482E-5
80-84	0.023648670100499945	4.2515703893030484E-4	3.202481591942556E-4	4.1687475895114316E-4	6.294532784162955E-4	6.90189998263482E-5
85-89	0.023745296700256834	4.472431188747363E-4	3.368127191525792E-4	4.500038788677902E-4	8.86203957770311E-4	6.90189998263482E-5
90-94	0.02387505241993037	4.8037223879138344E-4	3.561380391039567E-4	4.6656843882611384E-4	9.027685177286345E-4	6.90189998263482E-5
95-99	0.02394407141975672	5.245443986802463E-4	3.6718107907617243E-4	4.693291988191677E-4	9.16572317693904E-4	6.90189998263482E-5
100-104	0.023977200539673362	5.411089586385699E-4	4.1411399895808923E-4	4.91415278763599E-4	9.441799176244433E-4	6.90189998263482E-5
105-109	0.024018611939569175	5.714773185621631E-4	4.472431188747363E-4	5.079798387219228E-4	9.662659975688747E-4	6.90189998263482E-5
110-114	0.02406830561944414	6.156494784510259E-4	4.693291988191677E-4	5.521519986107856E-4	9.773090375410905E-4	6.90189998263482E-5
115-119	0.024090391699388574	6.349747984024034E-4	4.7208995881222165E-4	5.797595985413248E-4	9.800697975341445E-4	6.90189998263482E-5
120-124	0.024225668939048216	6.543001183537809E-4	4.941760387566531E-4	6.184102384440797E-4	9.883520775133062E-4	6.90189998263482E-5
125-129	0.024476898098416123	6.763861982982124E-4	5.328266786594081E-4	6.349747984024034E-4	0.0010049166374716297	6.90189998263482E-5
130-134	0.02468947661788128	7.233191181801291E-4	5.493912386177316E-4	6.349747984024034E-4	0.001029763477409115	6.90189998263482E-5
135-139	0.02491033741732559	7.840558380273154E-4	5.521519986107856E-4	6.681039183190505E-4	0.0010739356372979779	6.90189998263482E-5
140-144	0.02503733237700607	8.089026779648009E-4	5.631950385830014E-4	7.039937982287516E-4	0.0010905001972563015	6.90189998263482E-5
145-149	0.025249910896471224	8.254672379231244E-4	5.797595985413248E-4	7.398836781384526E-4	0.0011015432372285172	6.90189998263482E-5
150-154	0.02552874765576967	8.282279979161783E-4	5.797595985413248E-4	7.454051981245605E-4	0.0011291508371590567	8.00620397985639E-5
155-159	0.02581586669504728	8.503140778606097E-4	5.852811185274327E-4	7.950988779995312E-4	0.0011346723571451643	8.282279979161784E-5
160-164	0.026050531294456863	8.558355978467178E-4	5.935633985065944E-4	8.144241979509087E-4	0.0011650407170687575	8.282279979161784E-5
165-167	0.02636065666700992	8.604368645018075E-4	5.935633985065944E-4	8.190254646059987E-4	0.0011871267970131889	8.282279979161784E-5
>>END_MODULE
