##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479372
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7234372
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.19600042685115	39.0	39.0	39.0	37.0	39.0
2	39.94025106809548	41.0	40.0	41.0	38.0	41.0
3	39.996586849556536	41.0	40.0	41.0	38.0	41.0
4	40.01250004838015	41.0	40.0	41.0	38.0	41.0
5	40.010385282924354	41.0	40.0	41.0	38.0	41.0
6	39.99884634077429	41.0	40.0	41.0	38.0	41.0
7	40.01850443963899	41.0	40.0	41.0	38.0	41.0
8	39.9912914348336	41.0	40.0	41.0	38.0	41.0
9	39.97550748012405	41.0	40.0	41.0	38.0	41.0
10-14	39.90065738947347	41.0	40.0	41.0	38.0	41.0
15-19	39.756003700113844	40.8	39.8	41.0	37.6	41.0
20-24	39.541846728368405	40.0	39.0	41.0	36.8	41.0
25-29	39.26873273865375	40.0	38.4	41.0	35.8	41.0
30-34	39.18031077196473	40.0	38.2	41.0	35.0	41.0
35-39	39.04526714965722	40.0	37.8	41.0	35.0	41.0
40-44	38.519586302722615	39.8	36.6	41.0	35.0	41.0
45-49	37.873886551590104	38.8	35.2	40.8	34.4	41.0
50-54	37.18527626171284	37.2	35.0	39.8	34.0	41.0
55-59	36.49429990605957	35.8	35.0	39.0	33.8	40.8
60-64	35.78022943802172	35.0	34.8	37.0	32.8	39.6
65-69	35.41892390935938	35.0	34.8	36.2	33.0	39.0
70-74	35.2010579494668	35.0	35.0	36.0	33.0	37.6
75-79	35.03340262292291	35.0	35.0	35.0	33.0	37.0
80-84	34.96146756069497	35.0	35.0	35.0	33.0	37.0
85-89	34.792929310242826	35.0	33.8	35.0	32.4	37.8
90-94	39.29235388967483	40.6	38.8	40.6	36.4	40.6
95-99	39.542559826245665	41.0	39.0	41.0	36.4	41.0
100-104	39.402681289515456	40.4	39.0	41.0	36.0	41.0
105-109	39.22818037933797	40.0	38.6	41.0	35.2	41.0
110-114	39.001182828622426	40.0	38.0	41.0	35.0	41.0
115-119	38.702754207640865	40.0	37.8	41.0	34.6	41.0
120-124	38.37271971196016	40.0	37.0	41.0	34.0	41.0
125-129	37.90639166026617	39.2	35.8	40.6	33.6	40.8
130-134	37.65601972986481	38.8	35.0	40.6	33.4	41.0
135-139	37.32232619096804	37.8	35.0	40.0	33.6	41.0
140-144	36.86629930372312	36.6	35.0	39.6	33.6	41.0
145-149	36.19847865626826	35.4	35.0	38.4	33.0	40.6
150-154	35.5677772918829	35.0	35.0	36.8	33.0	39.2
155-159	35.06834527784408	35.0	35.0	36.0	33.0	37.4
160-164	34.718308169571095	35.0	34.6	35.0	33.0	36.4
165-169	34.50217274897304	35.0	34.0	35.0	33.0	36.0
170-174	34.37564069887892	35.0	34.0	35.0	33.0	35.6
175-178	34.07527398721972	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	45.0
30	521.0
31	3651.0
32	19138.0
33	73639.0
34	222406.0
35	582034.0
36	1367168.0
37	2296544.0
38	2198348.0
39	467458.0
40	3417.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.091302465507717	27.291767689026774	27.508344884670016	23.108584960795493
2	22.667440933366436	27.808757968210646	27.85825777275484	21.665543325668075
3	21.545436148431403	27.071942111906882	28.4590286482365	22.92359309142521
4	21.99233050221913	26.505922006775435	28.727344958207844	22.774402532797595
5	22.55069272080562	27.101799575692265	27.62984817479665	22.717659528705465
6	22.647148916312293	27.07389114079287	27.456509010042613	22.822450932852224
7	22.561751040726133	27.27848388222226	27.351385856298236	22.80837922075337
8	22.64126036095462	27.426305973759714	27.323477421398845	22.608956243886823
9	22.833094565775717	27.33218584833625	27.126597858114014	22.70812172777402
10-14	22.68891066149211	27.348231470540913	27.206286876041208	22.756570991925766
15-19	22.72100190590144	27.268147117676563	27.216524115707625	22.794326860714378
20-24	22.686422539509994	27.20821378828736	27.27806643064526	22.82729724155739
25-29	22.69708552449335	27.22670053461448	27.154146897615995	22.922067043276183
30-34	22.742242173888762	27.22483167854791	27.171021893814697	22.861904253748634
35-39	22.723506615363434	27.226927230172848	27.215003596718557	22.834562557745166
40-44	22.74320424772185	27.26730392078262	27.173308201458262	22.816183630037273
45-49	22.88432776196745	27.157024825375302	27.08289814236813	22.875749270289113
50-54	22.851219152125438	27.24201077854443	27.094678017663455	22.81209205166668
55-59	22.80409965094413	27.207489468332565	27.105542816985356	22.882868063737945
60-64	22.804265525742938	27.25951886355858	27.092422120399668	22.84379349029881
65-69	22.758973411928498	27.231444553860378	27.152164693770242	22.857417340440882
70-74	22.702423928434975	27.302270328371282	27.134053930320423	22.861251812873324
75-79	22.723807954581268	27.23583194228884	27.1391905199235	22.901169583206393
80-84	22.698594985162497	27.31871957925304	27.116653663925494	22.866031771658964
85-89	22.66441924744815	27.304766744093335	27.09844061101641	22.9323733974421
90-94	22.069202815732826	27.226344106151494	27.940713450167554	22.76373962794812
95-99	22.453913908795602	27.417197135737	27.307883462952375	22.821005492515027
100-104	22.517992234124016	27.41306140922844	27.231870406167218	22.837075950480326
105-109	22.45985895976748	27.425501309047963	27.291789549676416	22.822850181508148
110-114	22.514784125199327	27.28309356324291	27.278867668397783	22.923254643159982
115-119	22.51245804124056	27.347478692362387	27.19948264417655	22.9405806222205
120-124	22.43696817584048	27.375147399609013	27.2621341906222	22.925750233928312
125-129	22.47142916276252	27.36177719667407	27.234238844798668	22.93255479576474
130-134	22.58914293524211	27.366740637169578	27.1618422003803	22.88227422720801
135-139	22.679407234970654	27.3279448857651	27.129880169282877	22.862767709981377
140-144	22.66462791087358	27.40112046625554	27.17769575984994	22.756555863020935
145-149	22.600912126792856	27.42397633529311	27.22430252453037	22.750809013383662
150-154	22.50708188184456	27.569874288450514	27.27068803055014	22.65235579915479
155-159	22.446227330176313	27.558935222701482	27.36380198023044	22.631035466891767
160-164	22.403054872324933	27.712966258978618	27.391329949828823	22.492648918867626
165-169	22.25695910359102	27.750456131351616	27.491407878792636	22.501176886264734
170-174	22.21880050082624	27.86853225812332	27.516358263124914	22.39630897792553
175-178	22.124728451524085	27.993604888900347	27.556953863145665	22.3247127964299
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	5.0
11	9.0
12	13.0
13	32.0
14	72.0
15	139.5
16	263.0
17	488.5
18	917.0
19	1670.0
20	3040.5
21	5160.5
22	8217.5
23	12636.0
24	18379.5
25	25628.0
26	34619.5
27	45199.0
28	57129.0
29	70337.0
30	84886.5
31	100591.0
32	117291.5
33	134860.0
34	153940.5
35	173582.0
36	193389.5
37	214027.5
38	234432.0
39	253692.5
40	270796.0
41	284277.0
42	294759.5
43	301635.5
44	303547.0
45	300102.0
46	291827.0
47	280519.5
48	266098.5
49	249833.0
50	232551.0
51	214436.0
52	197664.5
53	182524.0
54	167845.5
55	154423.0
56	142808.5
57	132915.0
58	124145.0
59	115727.5
60	107789.5
61	100099.0
62	92312.0
63	84198.0
64	75486.5
65	66405.0
66	57291.0
67	47992.5
68	38880.0
69	30926.5
70	24037.5
71	17920.5
72	12919.0
73	9040.0
74	6182.5
75	4131.5
76	2662.5
77	1666.5
78	987.5
79	563.0
80	312.5
81	172.5
82	92.0
83	45.0
84	24.0
85	13.5
86	6.0
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1439.0
92-93	2954.0
94-95	4191.0
96-97	5504.0
98-99	6935.0
100-101	8786.0
102-103	9004.0
104-105	10926.0
106-107	11949.0
108-109	12639.0
110-111	13265.0
112-113	14642.0
114-115	16920.0
116-117	15963.0
118-119	17187.0
120-121	17740.0
122-123	18618.0
124-125	19492.0
126-127	20555.0
128-129	23655.0
130-131	23484.0
132-133	25318.0
134-135	108879.0
136-137	92607.0
138-139	98977.0
140-141	85528.0
142-143	88897.0
144-145	87363.0
146-147	93405.0
148-149	95140.0
150-151	93137.0
152-153	86771.0
154-155	88240.0
156-157	99930.0
158-159	91576.0
160-161	87753.0
162-163	89258.0
164-165	96439.0
166-167	93428.0
168-169	94156.0
170-171	104889.0
172-173	106326.0
174-175	107391.0
176-177	253679.0
178-179	4689437.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.6922729774258
#Duplication Level	Percentage of total
1	92.09278208670055
2	6.3725886273181125
3	0.8965820112775882
4	0.23870664211265222
5	0.12326073670444548
6	0.062055831890869745
7	0.04373825506105029
8	0.02726209925446798
9	0.02005886271865555
>10	0.10931900330441523
>50	0.013645843657224738
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.1468699701922985E-5	0.0	0.0	0.0	0.0	0.0
2	0.0015758105886730736	0.0	0.0	0.0	0.0	0.0
3	0.0015758105886730736	0.0	0.0	0.0	0.0	0.0
4	0.0015758105886730736	0.0	0.0	1.3822899900640995E-5	1.3822899900640995E-5	0.0
5	0.0015896334885737145	0.0	0.0	1.3822899900640995E-5	1.3822899900640995E-5	0.0
6	0.0015896334885737145	0.0	1.3822899900640995E-5	2.764579980128199E-5	1.3822899900640995E-5	0.0
7	0.0015896334885737145	0.0	1.3822899900640995E-5	4.1468699701922985E-5	2.764579980128199E-5	0.0
8	0.0015896334885737145	1.3822899900640995E-5	2.764579980128199E-5	5.529159960256398E-5	2.764579980128199E-5	0.0
9	0.0015896334885737145	4.1468699701922985E-5	2.764579980128199E-5	5.529159960256398E-5	2.764579980128199E-5	0.0
10-14	0.001597927228514099	4.6997859662179384E-5	4.1468699701922985E-5	5.529159960256397E-5	3.870411972179478E-5	0.0
15-19	0.0016034563884743554	6.911449950320498E-5	5.529159960256397E-5	6.358533954294859E-5	5.529159960256397E-5	0.0
20-24	0.0016034563884743554	9.676029930448697E-5	5.8056179582692176E-5	1.1334777918525615E-4	5.529159960256397E-5	0.0
25-29	0.0016034563884743554	9.676029930448697E-5	8.017281942371778E-5	1.3822899900640996E-4	6.082075956282038E-5	0.0
30-34	0.0016587479880769192	1.2993525906602535E-4	9.676029930448697E-5	1.4928731892692275E-4	8.846655936410236E-5	0.0
35-39	0.0018467394267256368	1.8799143864871754E-4	1.1058319920512795E-4	1.9628517858910215E-4	1.1058319920512797E-4	0.0
40-44	0.002015378805513457	1.9352059860897394E-4	1.1334777918525615E-4	2.3498929831089694E-4	1.5481647888717916E-4	5.529159960256398E-6
45-49	0.002184018184301277	1.9628517858910215E-4	1.3822899900640996E-4	2.432830382512815E-4	1.6587479880769194E-4	1.935205986089739E-5
50-54	0.0023167180233474305	2.211663984102559E-4	1.5205189890705095E-4	2.6816425807243526E-4	1.8799143864871754E-4	3.041037978141019E-5
55-59	0.002454947022353841	2.2393097839038412E-4	1.7693311872820473E-4	2.792225779929481E-4	2.2669555837051235E-4	4.1468699701922985E-5
60-64	0.0025434135817179434	2.3498929831089694E-4	2.0734349850961496E-4	3.4833707749615305E-4	2.3498929831089694E-4	6.082075956282038E-5
65-69	0.002637409301042302	2.405184582711533E-4	2.211663984102559E-4	3.5939539741666586E-4	2.4881219821153795E-4	8.293739940384597E-5
70-74	0.003383845895676916	2.488121982115379E-4	2.3498929831089688E-4	3.815120372576915E-4	2.7645799801281993E-4	8.293739940384597E-5
75-79	0.01762419737331727	2.626350981121789E-4	2.598705181320507E-4	4.2021615697948627E-4	2.792225779929481E-4	8.570197938397416E-5
80-84	0.02320035519323585	3.206912776948711E-4	2.792225779929481E-4	4.340390568801273E-4	4.063932570788452E-4	9.676029930448697E-5
85-89	0.023241823892937767	3.372787575756403E-4	3.0410379781410185E-4	4.8380149652243483E-4	6.717929351711524E-4	9.676029930448697E-5
90-94	0.02329711549254033	3.6215997739679406E-4	3.345141775955121E-4	5.335639361647424E-4	6.80086675111537E-4	9.676029930448697E-5
95-99	0.023335819612262127	3.7321829731730687E-4	3.6215997739679406E-4	5.777972158467935E-4	6.93909575012178E-4	9.676029930448697E-5
100-104	0.023402169531785207	3.842766172378197E-4	4.063932570788453E-4	6.165013355685885E-4	7.049678949326908E-4	9.676029930448697E-5
105-109	0.02345193197142751	3.870411972179479E-4	4.3956821684038364E-4	6.38617975409614E-4	7.049678949326908E-4	9.676029930448697E-5
110-114	0.023526575630890976	4.063932570788452E-4	4.506265367608964E-4	6.496762953301267E-4	7.049678949326908E-4	9.676029930448697E-5
115-119	0.023573573490553154	4.2021615697948627E-4	4.5615569672115286E-4	6.496762953301267E-4	7.077324749128189E-4	9.676029930448697E-5
120-124	0.02372286080948008	4.6168485668140926E-4	4.72743176601922E-4	6.552054552903832E-4	7.187907948333317E-4	9.676029930448697E-5
125-129	0.024018670867353792	4.6997859662179387E-4	5.197410362641014E-4	6.690283551910242E-4	7.270845347737163E-4	9.676029930448697E-5
130-134	0.024209426885982637	4.86566076502563E-4	5.750326358666654E-4	7.215553748134599E-4	7.602594945352548E-4	9.676029930448697E-5
135-139	0.024532882743657638	5.031535563833322E-4	5.943846957275628E-4	7.740823944358958E-4	7.713178144557676E-4	9.676029930448697E-5
140-144	0.024834221961491616	5.363285161448705E-4	6.082075956282038E-4	7.879052943365368E-4	7.906698743166648E-4	9.952487928461518E-5
145-149	0.025094092479623666	5.473868360653833E-4	6.275596554891011E-4	8.100219341775622E-4	8.238448340782033E-4	1.1058319920512795E-4
150-154	0.02536778589765636	5.529159960256399E-4	6.441471353698703E-4	8.34903153998716E-4	8.542552138596134E-4	1.1058319920512795E-4
155-159	0.025586187716086485	5.556805760057681E-4	6.52440875310255E-4	8.570197938397417E-4	8.708426937403827E-4	1.1058319920512795E-4
160-164	0.025782472894675584	5.667388959262808E-4	6.717929351711523E-4	8.5978437381987E-4	8.791364336807673E-4	1.1058319920512795E-4
165-167	0.025950190746803364	5.805617958269218E-4	7.003602616324771E-4	8.892732269412374E-4	8.846655936410237E-4	1.336213657061963E-4
>>END_MODULE
