##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479373
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7309461
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.2118380548169	39.0	39.0	39.0	37.0	39.0
2	39.9538269648063	41.0	40.0	41.0	38.0	41.0
3	40.00438828526481	41.0	40.0	41.0	38.0	41.0
4	40.01899880169003	41.0	40.0	41.0	38.0	41.0
5	40.032414154750946	41.0	40.0	41.0	38.0	41.0
6	40.011889795978114	41.0	40.0	41.0	38.0	41.0
7	40.02409233731461	41.0	40.0	41.0	38.0	41.0
8	40.01170948172512	41.0	40.0	41.0	38.0	41.0
9	39.99207711758774	41.0	40.0	41.0	38.0	41.0
10-14	39.918493525035565	41.0	40.0	41.0	38.0	41.0
15-19	39.773828494330836	41.0	40.0	41.0	37.8	41.0
20-24	39.56176210530435	40.0	39.0	41.0	37.0	41.0
25-29	39.29676341388237	40.0	38.6	41.0	35.8	41.0
30-34	39.2080382944789	40.0	38.4	41.0	35.0	41.0
35-39	39.0668901851997	40.0	37.8	41.0	35.0	41.0
40-44	38.54214862080802	39.8	36.6	41.0	35.0	41.0
45-49	37.895394229478754	38.8	35.2	41.0	34.4	41.0
50-54	37.19796376230751	37.2	35.0	39.8	34.0	41.0
55-59	36.495514074156766	35.8	35.0	39.0	34.0	40.8
60-64	35.76911879001749	35.0	34.8	37.0	32.8	39.6
65-69	35.400620647678394	35.0	34.8	36.2	33.0	39.0
70-74	35.17671746247773	35.0	35.0	36.0	33.0	37.4
75-79	35.0190093086207	35.0	35.0	35.0	33.0	37.0
80-84	34.949867137946285	35.0	35.0	35.0	33.0	37.0
85-89	34.77663176532442	35.0	34.2	35.0	32.4	37.4
90-94	39.30734555854517	40.6	38.8	40.6	36.6	40.6
95-99	39.56017305008436	41.0	39.0	41.0	36.4	41.0
100-104	39.42640299845131	40.6	39.0	41.0	36.0	41.0
105-109	39.252036554271385	40.0	38.6	41.0	35.2	41.0
110-114	39.02726357978187	40.0	38.0	41.0	35.0	41.0
115-119	38.729078467143665	40.0	38.0	41.0	34.8	41.0
120-124	38.397303278112	40.0	37.0	41.0	34.0	41.0
125-129	37.917704493050984	39.4	35.8	40.6	33.6	40.8
130-134	37.67063482726133	38.8	35.0	40.6	33.6	41.0
135-139	37.342581678160315	37.8	35.0	40.0	33.6	41.0
140-144	36.882813497285596	36.6	35.0	39.6	33.6	41.0
145-149	36.20888923362428	35.4	35.0	38.4	33.0	40.6
150-154	35.57693400126062	35.0	35.0	36.8	33.0	39.2
155-159	35.07980901412623	35.0	35.0	36.0	33.0	37.4
160-164	34.72781141782336	35.0	34.8	35.0	33.0	36.4
165-169	34.51285857339659	35.0	34.0	35.0	33.0	36.0
170-174	34.38555875847255	35.0	34.0	35.0	33.0	35.6
175-178	34.080824005844725	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	49.0
30	545.0
31	3820.0
32	20144.0
33	75598.0
34	224327.0
35	579365.0
36	1365730.0
37	2316137.0
38	2241427.0
39	479002.0
40	3316.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.099741690940004	27.315297256528215	27.50190472320736	23.08305632932442
2	22.734152354051822	27.777971590518096	27.826771358380597	21.66110469704948
3	21.54814151139188	27.079944745583838	28.45663996291929	22.91527378010499
4	21.967885183326104	26.524691765918174	28.707260357501053	22.800162693254673
5	22.56496067220278	27.060490506755556	27.663996018311067	22.7105528027306
6	22.69579111236793	27.04831450636374	27.416590635068715	22.83930374619962
7	22.583977122252925	27.265430378519017	27.348500799169734	22.802091700058323
8	22.683396217587042	27.41679584855846	27.289946550094456	22.609861383760034
9	22.818508779238307	27.332138443587016	27.11911316032742	22.73023961684726
10-14	22.70539510368822	27.329492557659172	27.19937899661822	22.765733342034387
15-19	22.73774769439224	27.278194657581455	27.187014199815827	22.797043448210477
20-24	22.71314396506117	27.216143570640845	27.253325518803646	22.817386945494338
25-29	22.704224018706714	27.239589895889726	27.12831219702793	22.92787388837563
30-34	22.75315238702279	27.234218774818007	27.165920989249415	22.84670784890979
35-39	22.739766995131376	27.24228776923497	27.188992457857015	22.82895277777664
40-44	22.749360041732213	27.260704995895047	27.15990686590981	22.830028096462925
45-49	22.886100630402158	27.163868854351914	27.081578244962245	22.868452270283676
50-54	22.85436368016739	27.2348015811289	27.100586486472807	22.81024825223091
55-59	22.815069401150097	27.202448990424877	27.100422315681005	22.88205929274402
60-64	22.80359659898315	27.247360646701583	27.085469092727905	22.86357366158736
65-69	22.769479172267285	27.23342528265764	27.149063385111432	22.84803215996364
70-74	22.717130579122045	27.30074898819489	27.12929722177873	22.852823210904333
75-79	22.71181144546773	27.240429903107767	27.142622417713152	22.90513623371135
80-84	22.69787881760365	27.31199195125331	27.11745503533024	22.8726741958128
85-89	22.68144805752435	27.29858466992299	27.083999764141296	22.93596750841136
90-94	22.076458554274573	27.223536656289554	27.93556429817659	22.76444049125928
95-99	22.46094974585081	27.426167830101278	27.290781814028474	22.822100610019437
100-104	22.50033408197912	27.395771572803024	27.258202588136182	22.845691757081678
105-109	22.455379824303538	27.429108148813864	27.293269687385223	22.82224233949737
110-114	22.52951843315026	27.28492969611034	27.260101611910088	22.92545025882932
115-119	22.50765118433123	27.355379152013764	27.1909026127526	22.946067050902407
120-124	22.46606323004753	27.34672850603623	27.24820652874429	22.939001735171953
125-129	22.49097348189891	27.332283575252735	27.248022650399896	22.928720292448457
130-134	22.613785069236698	27.35326141901447	27.147160846609957	22.885792665138876
135-139	22.68150336349313	27.337867478426	27.11801518549512	22.86261397258576
140-144	22.679688779678937	27.374348034094687	27.16362368842204	22.782339497804337
145-149	22.616701322638573	27.42524502391503	27.211450355922768	22.74660329752363
150-154	22.519925828677582	27.548241458743515	27.26883344801757	22.662999264561332
155-159	22.470233972162024	27.533577423261118	27.34100323958572	22.655185364991137
160-164	22.409907881235018	27.696361064994502	27.370424883118766	22.523306170651715
165-169	22.28450338468139	27.73062251819976	27.465898368219122	22.51897572889973
170-174	22.215883255547293	27.854892156460192	27.516560898942018	22.412663689050493
175-178	22.14646157537073	27.973234163931913	27.544712498517548	22.33559176217981
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	3.0
11	6.0
12	9.5
13	27.5
14	65.5
15	130.5
16	246.5
17	472.5
18	874.0
19	1688.5
20	3069.0
21	5084.5
22	8339.0
23	12913.0
24	18662.0
25	26113.5
26	35110.0
27	45679.5
28	57734.5
29	71034.0
30	85659.0
31	101529.5
32	118598.0
33	136250.0
34	155258.0
35	175405.5
36	195752.5
37	216694.0
38	237112.0
39	256000.0
40	272474.5
41	286505.0
42	297354.0
43	303568.5
44	305168.5
45	302183.0
46	295100.5
47	283304.0
48	268669.5
49	252648.0
50	234462.0
51	217358.0
52	200879.5
53	184432.5
54	170081.0
55	156706.5
56	144857.0
57	134840.5
58	125518.0
59	117282.5
60	109285.0
61	101084.0
62	93284.5
63	84940.5
64	76052.5
65	67001.0
66	57558.0
67	48403.5
68	39791.0
69	31599.5
70	24420.5
71	18308.5
72	13285.5
73	9317.0
74	6325.5
75	4200.0
76	2683.0
77	1664.0
78	1004.0
79	574.5
80	314.5
81	174.0
82	95.5
83	47.0
84	19.0
85	5.0
86	2.5
87	3.0
88	1.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1368.0
92-93	2742.0
94-95	3976.0
96-97	5393.0
98-99	7080.0
100-101	7460.0
102-103	8584.0
104-105	10101.0
106-107	12027.0
108-109	11871.0
110-111	12930.0
112-113	13939.0
114-115	14787.0
116-117	15230.0
118-119	16030.0
120-121	17365.0
122-123	17951.0
124-125	18301.0
126-127	19425.0
128-129	20213.0
130-131	21834.0
132-133	23533.0
134-135	109611.0
136-137	87475.0
138-139	85229.0
140-141	85385.0
142-143	91846.0
144-145	88660.0
146-147	93578.0
148-149	92333.0
150-151	90600.0
152-153	89827.0
154-155	90534.0
156-157	90057.0
158-159	89150.0
160-161	93002.0
162-163	89547.0
164-165	97212.0
166-167	94537.0
168-169	99901.0
170-171	104081.0
172-173	116940.0
174-175	112562.0
176-177	257365.0
178-179	4777889.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.64643922396957
#Duplication Level	Percentage of total
1	91.99009540511423
2	6.484903652566294
3	0.9005206645910002
4	0.25905826111356295
5	0.11501293113647196
6	0.04239069513259543
7	0.039556824853817875
8	0.0293454058227039
9	0.016882604529063018
>10	0.11164178239632382
>50	0.01059177274393313
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.104269794995828E-5	1.368089931665276E-5	0.0	0.0	1.368089931665276E-5	0.0
2	0.0016964315152649422	1.368089931665276E-5	1.368089931665276E-5	1.368089931665276E-5	1.368089931665276E-5	0.0
3	0.001710112414581595	5.472359726661104E-5	1.368089931665276E-5	2.736179863330552E-5	1.368089931665276E-5	0.0
4	0.001710112414581595	5.472359726661104E-5	1.368089931665276E-5	2.736179863330552E-5	1.368089931665276E-5	0.0
5	0.001710112414581595	5.472359726661104E-5	2.736179863330552E-5	4.104269794995828E-5	1.368089931665276E-5	0.0
6	0.001710112414581595	5.472359726661104E-5	4.104269794995828E-5	5.472359726661104E-5	1.368089931665276E-5	0.0
7	0.001710112414581595	5.472359726661104E-5	4.104269794995828E-5	5.472359726661104E-5	1.368089931665276E-5	0.0
8	0.001710112414581595	5.472359726661104E-5	4.104269794995828E-5	5.472359726661104E-5	1.368089931665276E-5	0.0
9	0.001764836011848206	6.84044965832638E-5	6.84044965832638E-5	5.472359726661104E-5	1.368089931665276E-5	0.0
10-14	0.0017949339903448422	7.934921603658602E-5	8.755775562657765E-5	6.84044965832638E-5	1.368089931665276E-5	0.0
15-19	0.0018332405084314698	1.0397483480656099E-4	1.368089931665276E-4	8.755775562657767E-5	1.368089931665276E-5	0.0
20-24	0.0018551299473381143	1.313366334398665E-4	1.9426877029646918E-4	9.850247507989986E-5	2.1889438906644414E-5	0.0
25-29	0.001868810846654767	1.9426877029646918E-4	2.2710292865643582E-4	1.1218337439655264E-4	2.736179863330552E-5	0.0
30-34	0.0019454238828280224	2.106858494764525E-4	2.3531146824642746E-4	1.368089931665276E-4	3.5570338223297175E-5	0.0
35-39	0.002060343437087906	2.2710292865643582E-4	2.572009071530719E-4	1.6417079179983313E-4	7.661303617325545E-5	4.3778877813288834E-5
40-44	0.0021506373725778136	2.3531146824642746E-4	2.7909034605971627E-4	1.9153259043313864E-4	9.576629521656932E-5	6.0195956993272146E-5
45-49	0.0023148081643776467	2.517285474264108E-4	2.8729888564970794E-4	2.4625618769974966E-4	1.2312809384987483E-4	6.84044965832638E-5
50-54	0.0024762427763141497	2.8729888564970794E-4	2.9277124537636906E-4	2.7088180646972466E-4	1.6143461193650257E-4	6.84044965832638E-5
55-59	0.0025856899708473716	2.8729888564970794E-4	3.091883245563524E-4	2.9277124537636906E-4	1.9153259043313864E-4	6.84044965832638E-5
60-64	0.0027525969425105353	2.9277124537636906E-4	3.256054037363357E-4	3.064521446930218E-4	2.1342202933978305E-4	6.84044965832638E-5
65-69	0.00277448638141718	3.0097978496636073E-4	3.2834158359966627E-4	3.256054037363357E-4	2.517285474264108E-4	6.84044965832638E-5
70-74	0.003554297642466387	3.0097978496636073E-4	3.2834158359966627E-4	3.4749484264298014E-4	2.87298885649708E-4	6.84044965832638E-5
75-79	0.017448618988458932	3.064521446930218E-4	3.338139433263274E-4	3.5570338223297175E-4	3.5296720236964116E-4	7.934921603658602E-5
80-84	0.023547563903822732	3.283415835996662E-4	3.611757419596328E-4	3.6938428154962454E-4	5.061932747161521E-4	9.576629521656932E-5
85-89	0.023624176939995988	3.42022482916319E-4	3.8032900100294675E-4	4.0769079963625223E-4	8.509519374958018E-4	9.576629521656932E-5
90-94	0.023667955817809276	3.775928211396162E-4	3.830651808662773E-4	4.21371698952905E-4	8.618966569491239E-4	9.576629521656932E-5
95-99	0.02370352615603257	3.9674608018292997E-4	3.858013607296078E-4	4.5694203717620217E-4	8.618966569491239E-4	9.576629521656932E-5
100-104	0.023810237170702464	4.131631593629133E-4	4.268440586795661E-4	4.788314760828465E-4	8.728413764024461E-4	9.576629521656932E-5
105-109	0.02389779492632904	4.3505259826955776E-4	4.377887781328883E-4	4.979847351261604E-4	8.810499159924377E-4	9.576629521656932E-5
110-114	0.023966199422912303	4.377887781328883E-4	4.4873349758621056E-4	5.226103538961354E-4	8.892584555824295E-4	9.576629521656932E-5
115-119	0.0240017697611356	4.569420371762022E-4	4.5420585731287163E-4	5.581806921194326E-4	8.947308153090906E-4	9.576629521656932E-5
120-124	0.024133106394575467	4.925123753994993E-4	4.678867566295244E-4	5.800701310260769E-4	9.248287938057266E-4	9.576629521656932E-5
125-129	0.024382098762138548	5.089294545794828E-4	4.8156765594617717E-4	5.937510303427298E-4	9.57662952165693E-4	9.576629521656932E-5
130-134	0.02465024438874494	5.581806921194326E-4	4.952485552628299E-4	6.320575484293575E-4	9.932332903889902E-4	1.0123865494323042E-4
135-139	0.02504151810920121	5.663892317094243E-4	5.171379941694744E-4	6.621555269259935E-4	0.0010260674487489571	1.0944719453322208E-4
140-144	0.02533428935457758	5.745977712994159E-4	5.198741740328049E-4	6.703640665159852E-4	0.0010780548661522376	1.0944719453322208E-4
145-149	0.025575073182550666	5.882786706160687E-4	5.335550733494576E-4	6.867811456959686E-4	0.0010972081251955513	1.2312809384987483E-4
150-154	0.025763869593120475	5.992233900693909E-4	5.527083323927715E-4	7.114067644659435E-4	0.0011136252043755345	1.2312809384987483E-4
155-159	0.02601559814054689	6.129042893860436E-4	5.663892317094243E-4	7.114067644659435E-4	0.0011273061036921874	1.2312809384987483E-4
160-164	0.026245437249066656	6.156404692493741E-4	5.828063108894075E-4	7.223514839192658E-4	0.0011628764419154844	1.3407281330319706E-4
165-167	0.026504462276128612	6.156404692493741E-4	5.882786706160687E-4	7.387685630992491E-4	0.00119023824054879	1.5048989248318037E-4
>>END_MODULE
