##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479374
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8449888
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.1909667915125	39.0	39.0	39.0	37.0	39.0
2	39.92921586652983	41.0	40.0	41.0	38.0	41.0
3	39.984932344665395	41.0	40.0	41.0	38.0	41.0
4	40.003612592261575	41.0	40.0	41.0	38.0	41.0
5	40.01198193396173	41.0	40.0	41.0	38.0	41.0
6	39.998141158794056	41.0	40.0	41.0	38.0	41.0
7	40.02340551732757	41.0	40.0	41.0	38.0	41.0
8	39.99504076267046	41.0	40.0	41.0	38.0	41.0
9	39.977300764223145	41.0	40.0	41.0	38.0	41.0
10-14	39.91303366387815	41.0	40.0	41.0	38.0	41.0
15-19	39.784508481059156	41.0	40.0	41.0	37.8	41.0
20-24	39.606062967935195	40.4	39.2	41.0	37.0	41.0
25-29	39.375878402175275	40.0	38.8	41.0	36.0	41.0
30-34	39.2808354382922	40.0	38.6	41.0	35.2	41.0
35-39	39.123261468081	40.0	38.0	41.0	35.0	41.0
40-44	38.66351589512192	40.0	36.8	41.0	35.0	41.0
45-49	38.10214757876081	39.0	35.4	41.0	34.8	41.0
50-54	37.486874571591954	37.8	35.0	40.2	34.0	41.0
55-59	36.831967737323865	36.6	35.0	39.4	34.0	41.0
60-64	36.09639093441238	35.4	34.8	38.4	33.4	40.2
65-69	35.663706856232885	35.0	35.0	37.0	33.0	39.0
70-74	35.35798985738036	35.0	35.0	36.0	33.0	38.6
75-79	35.11934198417778	35.0	35.0	35.8	33.0	37.0
80-84	35.007841784411816	35.0	35.0	35.0	33.0	37.0
85-89	34.81768208051989	35.0	34.4	35.0	32.6	37.2
90-94	39.418973635151744	40.6	39.8	40.6	36.8	40.6
95-99	39.68918399693301	41.0	39.8	41.0	37.0	41.0
100-104	39.57319772454483	41.0	39.0	41.0	36.4	41.0
105-109	39.42075988140541	40.4	39.0	41.0	36.0	41.0
110-114	39.22227159443675	40.0	38.4	41.0	35.0	41.0
115-119	38.9667359011381	40.0	38.0	41.0	35.0	41.0
120-124	38.67149043430873	40.0	37.6	41.0	34.8	41.0
125-129	38.23722018403198	39.6	36.6	40.6	34.0	41.0
130-134	38.05565942731563	39.0	35.6	41.0	34.0	41.0
135-139	37.75472219836307	39.0	35.0	41.0	34.0	41.0
140-144	37.31288373175041	37.2	35.0	40.4	34.0	41.0
145-149	36.67397472710607	36.2	35.0	39.2	34.0	41.0
150-154	36.023169681765154	35.2	35.0	37.6	33.6	39.8
155-159	35.4561804586465	35.0	35.0	36.4	33.0	38.6
160-164	35.01464167180463	35.0	35.0	35.8	33.0	37.0
165-169	34.72282678993671	35.0	35.0	35.0	33.0	36.0
170-174	34.550723495012235	35.0	34.4	35.0	33.0	36.0
175-178	34.24225201582066	35.0	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	60.0
30	615.0
31	4388.0
32	22807.0
33	85456.0
34	245566.0
35	594027.0
36	1363986.0
37	2359915.0
38	2745983.0
39	1007317.0
40	19765.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.78822819900098	28.52480411574686	29.10622010611265	21.580747579139512
2	21.54903118242514	29.263736986809768	29.220458306666313	19.96677352409878
3	19.832475886070917	28.009495510473037	30.43202466115527	21.72600394230077
4	20.426779621221016	27.08782648953453	30.94418529571043	21.54120859353402
5	21.251157411790548	27.983495165853085	29.279263819828145	21.486083602528222
6	21.55509043433475	28.004276506386834	28.81770740629935	21.62292565297907
7	21.462734180618725	28.39953618320148	28.524460915931666	21.613268720248126
8	21.52524388488936	28.578142100818376	28.461986715090188	21.434627299202074
9	21.8267863432036	28.40939430203099	28.168420693860085	21.595398660905328
10-14	21.631784942001598	28.5503902536933	28.243269023210722	21.574555781094375
15-19	21.652369830227336	28.45806476961588	28.2954282944342	21.594137105722584
20-24	21.556988684347058	28.38238566002295	28.45315109502043	21.60747456060956
25-29	21.583642292063516	28.410831007464243	28.276540470122207	21.728986230350035
30-34	21.684550138416036	28.41597663779686	28.22932801002806	21.670145213759046
35-39	21.610537323098246	28.449454004597456	28.2914329752063	21.648575697097996
40-44	21.588217500634325	28.468867279660987	28.298339575625143	21.64457564407954
45-49	21.75707654349975	28.375358347944967	28.174006566714255	21.69355854184103
50-54	21.710898416641736	28.44202432032235	28.228591905596858	21.618485357439056
55-59	21.63941107858471	28.431733059657123	28.246196872668612	21.68265898908956
60-64	21.664698987726226	28.419863079842006	28.234899681510573	21.680538250921195
65-69	21.629368341923584	28.43215436701646	28.277333380040066	21.661143911019888
70-74	21.583718032712387	28.48306391753358	28.271562889354275	21.66165516039976
75-79	21.60669821895864	28.44295214327101	28.257988744939578	21.69236089283077
80-84	21.591471981640467	28.49516111929531	28.258279873058672	21.65508702600555
85-89	21.57538892823195	28.47759402254799	28.229900798685144	21.717116250534918
90-94	20.720053015638495	28.219738382066577	29.669036732629078	21.391171869665847
95-99	21.37516906425053	28.463279334869966	28.532498124563855	21.629053476315644
100-104	21.496969539268502	28.52764556530678	28.32963637441621	21.645748521008507
105-109	21.379090131960726	28.501599427885854	28.47336848033062	21.6459419598228
110-114	21.424343040208328	28.337630989557933	28.508782500937933	21.729243469295803
115-119	21.46728679035827	28.413566372846322	28.365305976300682	21.753840860494726
120-124	21.399880808643996	28.457564994692397	28.400102224821477	21.74245197184213
125-129	21.42759088083795	28.427740615099783	28.414830475462487	21.72983802859978
130-134	21.491571813221864	28.453935837503973	28.363868775218563	21.690623574055596
135-139	21.542858305690153	28.4281742838197	28.338759092792245	21.690208317697905
140-144	21.5458740246894	28.470610316582057	28.396195585649515	21.587320073079027
145-149	21.457468887821218	28.540624812750377	28.454070026544052	21.547836272884354
150-154	21.34807967963974	28.653029874921344	28.525952920611264	21.472937524827657
155-159	21.33636172058493	28.653572065783766	28.559310805387234	21.450755408244067
160-164	21.248133904366174	28.786257090870173	28.62526336470495	21.340345640058704
165-169	21.136373906069668	28.873514867125845	28.696149441865117	21.293961784939373
170-174	21.121526231333092	28.95343780670273	28.727548156604154	21.19748780536002
175-178	21.022187894086198	29.089962954603344	28.77574592945844	21.11210322185202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	2.0
7	4.5
8	12.5
9	38.0
10	86.0
11	194.0
12	377.5
13	686.0
14	1288.5
15	2246.0
16	3770.0
17	6144.0
18	9687.0
19	14650.0
20	21034.0
21	29178.0
22	39002.0
23	50491.0
24	63341.5
25	77015.5
26	91092.5
27	105992.0
28	121654.0
29	137807.5
30	154296.5
31	170780.0
32	186894.5
33	203026.5
34	220278.5
35	237560.0
36	254534.5
37	271480.5
38	287349.0
39	301923.0
40	314748.5
41	324174.0
42	329772.0
43	330304.5
44	325404.0
45	315961.5
46	301952.0
47	284685.0
48	266263.5
49	246218.0
50	225825.0
51	207108.5
52	189774.0
53	174149.0
54	160905.5
55	149268.5
56	138684.5
57	129784.5
58	121720.0
59	113753.0
60	105829.0
61	98063.5
62	89833.5
63	80897.5
64	71321.5
65	61674.5
66	52354.0
67	43403.0
68	35096.5
69	27420.5
70	20753.0
71	15416.5
72	11289.0
73	8008.5
74	5498.5
75	3692.5
76	2400.5
77	1498.0
78	922.0
79	544.5
80	296.5
81	163.0
82	94.0
83	47.0
84	20.5
85	7.5
86	2.5
87	2.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1510.0
92-93	3316.0
94-95	5106.0
96-97	6654.0
98-99	7324.0
100-101	9642.0
102-103	9572.0
104-105	11282.0
106-107	11976.0
108-109	12944.0
110-111	14104.0
112-113	15328.0
114-115	16013.0
116-117	16265.0
118-119	18488.0
120-121	20413.0
122-123	18723.0
124-125	20354.0
126-127	21401.0
128-129	23706.0
130-131	23895.0
132-133	25164.0
134-135	121007.0
136-137	93348.0
138-139	91362.0
140-141	91208.0
142-143	94227.0
144-145	90778.0
146-147	95473.0
148-149	87610.0
150-151	91111.0
152-153	87955.0
154-155	92583.0
156-157	87724.0
158-159	92575.0
160-161	102075.0
162-163	87226.0
164-165	101424.0
166-167	105607.0
168-169	103564.0
170-171	104480.0
172-173	103908.0
174-175	110336.0
176-177	254883.0
178-179	5846244.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.19842474854322
#Duplication Level	Percentage of total
1	92.83095025426964
2	6.176142401595676
3	0.6300861241802904
4	0.15158913714743727
5	0.07880201842315646
6	0.045295906698686016
7	0.013461831768176865
8	0.010416221193042176
9	0.009429679943951044
>10	0.05218264709639275
>50	0.0016437776835572142
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.1834476385959198E-5	1.1834476385959198E-5	2.3668952771918396E-5	0.0
2	2.2485505133322477E-4	1.1834476385959198E-5	1.1834476385959198E-5	1.1834476385959198E-5	3.550342915787759E-5	0.0
3	2.4852400410514317E-4	2.3668952771918396E-5	1.1834476385959198E-5	1.1834476385959198E-5	3.550342915787759E-5	0.0
4	2.4852400410514317E-4	3.550342915787759E-5	1.1834476385959198E-5	1.1834476385959198E-5	3.550342915787759E-5	0.0
5	2.4852400410514317E-4	4.733790554383679E-5	2.3668952771918396E-5	2.3668952771918396E-5	3.550342915787759E-5	0.0
6	2.4852400410514317E-4	7.100685831575518E-5	2.3668952771918396E-5	7.100685831575518E-5	3.550342915787759E-5	0.0
7	2.603584804911024E-4	7.100685831575518E-5	2.3668952771918396E-5	7.100685831575518E-5	3.550342915787759E-5	0.0
8	2.7219295687706157E-4	9.467581108767358E-5	4.733790554383679E-5	1.0651028747363278E-4	3.550342915787759E-5	0.0
9	2.7219295687706157E-4	9.467581108767358E-5	4.733790554383679E-5	1.5384819301746957E-4	4.733790554383679E-5	0.0
10-14	3.195308624208983E-4	1.065102874736328E-4	7.100685831575517E-5	2.0591988911569003E-4	4.9704800821028635E-5	0.0
15-19	3.5503429157877594E-4	1.3017924024555117E-4	1.065102874736328E-4	2.4615710882795137E-4	1.017764969192491E-4	0.0
20-24	3.976384065682291E-4	1.5858198357185323E-4	1.372799260771267E-4	3.0769638603493914E-4	1.372799260771267E-4	0.0
25-29	4.213073593401474E-4	2.0118609856130637E-4	1.5384819301746957E-4	3.266315482524739E-4	1.869847268981553E-4	0.0
30-34	6.366948295646049E-4	2.3668952771918397E-4	1.91718517452539E-4	3.976384065682291E-4	2.4615710882795137E-4	2.3668952771918397E-6
35-39	0.0010461677125187933	2.4379021355075948E-4	2.035529938384982E-4	4.213073593401475E-4	3.83437034905078E-4	2.3668952771918393E-5
40-44	0.0014106695852063365	2.50890899382335E-4	2.224881560560329E-4	4.5917768377521696E-4	4.5681078849802505E-4	3.550342915787759E-5
45-49	0.001815408677606141	2.6509227104548605E-4	2.437902135507595E-4	4.994149034874782E-4	6.011914004067272E-4	3.550342915787759E-5
50-54	0.0022106801888971783	2.8166053798582894E-4	2.6272537576829425E-4	5.46752809031315E-4	7.219030595435112E-4	3.550342915787759E-5
55-59	0.002463937983556705	2.9822880492617177E-4	2.911281190945963E-4	5.822562381891926E-4	8.473485092346786E-4	3.550342915787759E-5
60-64	0.0026911599301671215	3.5503429157877594E-4	3.005957002033636E-4	6.224934579014538E-4	9.940960164205727E-4	3.550342915787759E-5
65-69	0.0029018136098371955	4.047390923998046E-4	3.621349774103515E-4	6.698313634452906E-4	0.001121908361388932	4.733790554383679E-5
70-74	0.005084091055408071	4.5917768377521696E-4	4.071059876769964E-4	7.38471326483854E-4	0.0012615551827432506	5.443859137541231E-5
75-79	0.04196505326461132	4.8521353182432716E-4	4.1420667350857196E-4	8.000106036908418E-4	0.0013609647843853079	5.917238192979599E-5
80-84	0.057977099814814116	5.183500657050129E-4	4.3314183572610665E-4	9.01787100610091E-4	0.0015408488254518877	5.917238192979599E-5
85-89	0.05812148042672281	5.75155552357617E-4	4.5917768377521696E-4	9.656932730942706E-4	0.001945587917851692	5.917238192979599E-5
90-94	0.058256393457522745	6.295941437330293E-4	4.710121601611761E-4	0.0010059304928065316	0.0020000265092271047	5.917238192979599E-5
95-99	0.05835580305916481	6.863996303856335E-4	4.923142176559026E-4	0.001048534607795985	0.0020402637289393655	5.917238192979599E-5
100-104	0.058521485728568245	7.361044312066622E-4	5.0178179876467E-4	0.0010887718275082463	0.0020568319958797084	5.917238192979599E-5
105-109	0.05866349944519975	7.739747556417315E-4	5.301845420909721E-4	0.0011290090472205075	0.0020734002628200517	5.917238192979599E-5
110-114	0.05877474352322776	8.071112895224172E-4	5.443859137541231E-4	0.0011763469527643444	0.00209706921559197	5.917238192979599E-5
115-119	0.0589096565540277	8.284133470171439E-4	5.46752809031315E-4	0.001237886229971332	0.0021515078069673827	5.917238192979599E-5
120-124	0.05927889221726962	8.40247823403103E-4	5.585872854172742E-4	0.0013065261930098955	0.002229615351114713	5.917238192979599E-5
125-129	0.05997475942876403	8.568160903434459E-4	5.775224476348088E-4	0.001375166156048459	0.002314823581093619	6.863996303856336E-5
130-134	0.06080553967105836	8.757512525609807E-4	6.082920862383028E-4	0.001387000632434418	0.0024142331827356764	7.100685831575518E-5
135-139	0.061607917170026394	8.875857289469399E-4	6.34327934287413E-4	0.0014059357946519528	0.0025822827474162972	7.100685831575518E-5
140-144	0.06227774853347169	8.923195195013235E-4	6.461624106733723E-4	0.001448539909641406	0.002653289605732052	7.100685831575518E-5
145-149	0.06311326256632041	9.112546817188584E-4	6.603637823365232E-4	0.0014745757576905161	0.002686426139612738	7.100685831575518E-5
150-154	0.06379492840615165	9.349236344907766E-4	6.745651539996741E-4	0.0015053453962940101	0.002731397149879383	7.100685831575518E-5
155-159	0.06447422735070571	9.538587967083114E-4	7.053347926031684E-4	0.0015550501971150388	0.0027976702176407543	7.100685831575518E-5
160-164	0.06518429593386328	9.58592587262695E-4	7.195361642663193E-4	0.0015787191498869574	0.002878144657065277	7.100685831575518E-5
165-167	0.06578785422954718	9.58592587262695E-4	7.219030595435112E-4	0.001641047392186342	0.0029270604927939085	7.100685831575518E-5
>>END_MODULE
