##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479375
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8458986
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.234109974883516	39.0	39.0	39.0	37.0	39.0
2	39.94867942800709	41.0	40.0	41.0	38.0	41.0
3	39.98725934763339	41.0	40.0	41.0	38.0	41.0
4	40.00368743960564	41.0	40.0	41.0	38.0	41.0
5	40.0039412525331	41.0	40.0	41.0	38.0	41.0
6	39.990421192327304	41.0	40.0	41.0	38.0	41.0
7	40.020845406293375	41.0	40.0	41.0	38.0	41.0
8	39.9990093375258	41.0	40.0	41.0	38.0	41.0
9	39.978407104586765	41.0	40.0	41.0	38.0	41.0
10-14	39.916476726643126	41.0	40.0	41.0	38.0	41.0
15-19	39.79016927087952	41.0	40.0	41.0	37.8	41.0
20-24	39.60903096423141	40.4	39.2	41.0	37.0	41.0
25-29	39.380049570953304	40.0	38.8	41.0	36.2	41.0
30-34	39.28310814085754	40.0	38.8	41.0	35.2	41.0
35-39	39.12458672942596	40.0	38.0	41.0	35.0	41.0
40-44	38.66933379485437	40.0	36.8	41.0	35.0	41.0
45-49	38.100804753666694	39.0	35.4	41.0	34.6	41.0
50-54	37.47169095681208	37.8	35.0	40.2	34.0	41.0
55-59	36.80614179997461	36.4	35.0	39.4	34.0	41.0
60-64	36.06438731545365	35.4	34.8	38.2	33.4	40.0
65-69	35.62577959107629	35.0	35.0	36.8	33.0	39.0
70-74	35.310304592063396	35.0	35.0	36.0	33.0	38.2
75-79	35.058879775897495	35.0	35.0	35.6	33.0	37.0
80-84	34.94193599563825	35.0	35.0	35.0	33.0	37.0
85-89	34.74129532783244	35.0	34.2	35.0	32.6	37.0
90-94	39.441533760703685	40.6	39.8	40.6	36.8	40.6
95-99	39.709059347552724	41.0	39.8	41.0	37.0	41.0
100-104	39.59243090007062	41.0	39.0	41.0	36.6	41.0
105-109	39.447663818958496	40.4	39.0	41.0	36.0	41.0
110-114	39.25722417195085	40.0	38.6	41.0	35.2	41.0
115-119	39.00135462557789	40.0	38.0	41.0	35.0	41.0
120-124	38.70552910989999	40.0	37.8	41.0	34.8	41.0
125-129	38.274306657650484	39.6	36.6	40.6	34.0	41.0
130-134	38.087749328392235	39.0	35.6	41.0	34.0	41.0
135-139	37.772314368118444	39.0	35.0	41.0	34.0	41.0
140-144	37.33079039100984	37.4	35.0	40.4	34.0	41.0
145-149	36.68367946041557	36.2	35.0	39.2	34.0	41.0
150-154	36.027619936558196	35.2	35.0	37.6	33.4	39.8
155-159	35.455378182243855	35.0	35.0	36.4	33.0	38.6
160-164	35.01721779219646	35.0	35.0	35.8	33.0	37.0
165-169	34.72704763030421	35.0	35.0	35.0	33.0	36.0
170-174	34.5555867379809	35.0	34.6	35.0	33.0	36.0
175-178	34.235377376151604	34.75	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	62.0
30	542.0
31	4253.0
32	22747.0
33	86225.0
34	248771.0
35	600533.0
36	1376263.0
37	2371896.0
38	2742426.0
39	988927.0
40	16338.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.772785296015385	28.492883189545417	29.1139150720902	21.620416442349
2	21.553978219138795	29.247607219115864	29.199646387876747	19.998768173868594
3	19.842614705828808	27.96812762191591	30.42579808029	21.76345959196528
4	20.395423281230162	27.06875268501449	30.977365372161632	21.55845866159372
5	21.266780675603435	27.95205004476896	29.26932377001215	21.511845509615455
6	21.58915974089566	27.971224919866284	28.792056163705677	21.647559175532386
7	21.501265045243013	28.326480266074444	28.543444805323006	21.628809883359544
8	21.556579003677275	28.56688733141301	28.441245794708724	21.43528787020099
9	21.85649674795537	28.438408575212204	28.10426687075732	21.600827806075102
10-14	21.652968807372417	28.53198243855706	28.21624010253711	21.59880865153341
15-19	21.671994728446176	28.431781303338248	28.27323274917348	21.622991219042092
20-24	21.579068696886363	28.369478327544222	28.412381815030784	21.639071160538627
25-29	21.602818588421826	28.394403300821164	28.246458854524644	21.756319256232366
30-34	21.6868310220634	28.415252135421433	28.205971732309287	21.69194511020588
35-39	21.649575965724495	28.417770167724594	28.26295255719775	21.669701309353155
40-44	21.610533461102786	28.461472805369343	28.266820633111344	21.661173100416526
45-49	21.781932255237212	28.341127411725235	28.163742084453148	21.713198248584405
50-54	21.723752705111462	28.41206499218701	28.216379599162355	21.64780270353917
55-59	21.670072512237283	28.40341856577136	28.21750266521306	21.709006256778295
60-64	21.68514760516213	28.40281329227877	28.219831549549788	21.69220755300931
65-69	21.65308466050186	28.40794629521789	28.253717407736577	21.685251636543672
70-74	21.595909958947797	28.467624842977635	28.245777921845477	21.690687276229088
75-79	21.632583385289916	28.41028700130252	28.238746346193267	21.718383267214296
80-84	21.618851242926755	28.473824167577533	28.221167407062737	21.68615718243298
85-89	21.592514752950294	28.47167024510976	28.205416110157884	21.730398891782066
90-94	20.730710154815384	28.18535514835341	29.684882768112736	21.39905192871847
95-99	21.401225309733753	28.442332500198226	28.499362192216815	21.657079997851206
100-104	21.518451011892132	28.502037696471316	28.308846922729337	21.670664368907214
105-109	21.425477145399466	28.481232409443763	28.43970415838346	21.65358628677331
110-114	21.451319435042045	28.307887768758665	28.473547232161202	21.767245564038088
115-119	21.497266235128365	28.397651697954213	28.34452717507525	21.760554891842173
120-124	21.424227605894657	28.42710614774329	28.3686814916851	21.77998475467695
125-129	21.447083428087534	28.400717609466735	28.386404493363443	21.765794469082287
130-134	21.528400491635757	28.422364227132913	28.32774966009437	21.721485621136964
135-139	21.5646587639644	28.434730005999516	28.304223056787052	21.696388173249034
140-144	21.564882536886376	28.446796037975457	28.372233517032136	21.61608790810603
145-149	21.47907590293328	28.522968996334562	28.420584906291374	21.577370194440782
150-154	21.38364249459899	28.620003950811423	28.503196059395382	21.493157495194207
155-159	21.361491047816543	28.626163242942077	28.521128720031815	21.491216989209565
160-164	21.280159431775388	28.765209548425368	28.59302479100331	21.36160622879593
165-169	21.159105771692165	28.84340078228765	28.676339625775782	21.321153820244398
170-174	21.152540531065977	28.919893463259083	28.710832069259624	21.216733936415313
175-178	21.058066144194456	29.04457134364546	28.759667393215427	21.137695118944656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	3.0
7	8.5
8	17.0
9	41.0
10	85.5
11	200.5
12	389.5
13	708.5
14	1283.5
15	2215.5
16	3677.0
17	6004.5
18	9539.0
19	14452.0
20	21066.5
21	29340.0
22	39186.5
23	50442.5
24	62963.0
25	76681.0
26	91082.0
27	106260.5
28	121603.5
29	137175.5
30	153599.5
31	170104.0
32	186580.0
33	203458.5
34	220142.5
35	237096.5
36	254138.0
37	270582.0
38	286603.5
39	301187.5
40	314453.5
41	323681.0
42	328684.0
43	329502.5
44	324319.0
45	315328.0
46	301979.0
47	285138.5
48	266645.0
49	246578.0
50	226682.5
51	207608.5
52	190420.0
53	175438.5
54	161668.0
55	149751.5
56	139738.0
57	131046.5
58	122725.0
59	114741.5
60	106979.0
61	98986.0
62	90631.0
63	81662.5
64	72252.0
65	62753.5
66	53363.0
67	44093.5
68	35393.5
69	27761.0
70	21303.5
71	15962.0
72	11483.5
73	7989.0
74	5549.0
75	3693.5
76	2394.5
77	1528.0
78	910.0
79	515.0
80	300.0
81	175.0
82	90.0
83	46.5
84	25.5
85	11.0
86	4.5
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1494.0
92-93	2836.0
94-95	4195.0
96-97	5565.0
98-99	6790.0
100-101	7809.0
102-103	8953.0
104-105	10237.0
106-107	11267.0
108-109	12376.0
110-111	13226.0
112-113	13972.0
114-115	14683.0
116-117	15426.0
118-119	16144.0
120-121	16978.0
122-123	17794.0
124-125	18558.0
126-127	18992.0
128-129	19990.0
130-131	21003.0
132-133	23060.0
134-135	105677.0
136-137	81616.0
138-139	79670.0
140-141	81626.0
142-143	81014.0
144-145	86389.0
146-147	83937.0
148-149	90955.0
150-151	89329.0
152-153	83793.0
154-155	83782.0
156-157	83065.0
158-159	87462.0
160-161	84564.0
162-163	85714.0
164-165	88456.0
166-167	91932.0
168-169	95031.0
170-171	95399.0
172-173	105023.0
174-175	108622.0
176-177	262697.0
178-179	6041885.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.19171433557378
#Duplication Level	Percentage of total
1	92.84145418138658
2	6.097652136376153
3	0.6988473564954524
4	0.1617590971020601
5	0.054238746092363
6	0.041176631446615534
7	0.020709649269274124
8	0.014582016335011591
9	0.014667606476135353
>10	0.05326956956286889
>50	0.0016430094575497676
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.1821747902171727E-5	0.0	0.0	0.0	0.0
2	2.1279146223909107E-4	2.3643495804343454E-5	1.1821747902171727E-5	0.0	0.0	0.0
3	2.1279146223909107E-4	3.546524370651518E-5	1.1821747902171727E-5	1.1821747902171727E-5	0.0	0.0
4	2.1279146223909107E-4	4.728699160868691E-5	2.3643495804343454E-5	3.546524370651518E-5	1.1821747902171727E-5	0.0
5	2.1279146223909107E-4	4.728699160868691E-5	3.546524370651518E-5	5.910873951085863E-5	1.1821747902171727E-5	0.0
6	2.1279146223909107E-4	4.728699160868691E-5	4.728699160868691E-5	8.275223531520208E-5	1.1821747902171727E-5	0.0
7	2.246132101412628E-4	4.728699160868691E-5	4.728699160868691E-5	8.275223531520208E-5	1.1821747902171727E-5	0.0
8	2.246132101412628E-4	5.910873951085863E-5	5.910873951085863E-5	1.0639573111954553E-4	1.1821747902171727E-5	0.0
9	2.246132101412628E-4	5.910873951085863E-5	7.093048741303036E-5	1.0639573111954553E-4	1.1821747902171727E-5	0.0
10-14	2.364349580434345E-4	6.620178825216166E-5	7.093048741303036E-5	1.465896739869294E-4	3.546524370651518E-5	0.0
15-19	2.6007845384777795E-4	1.1348877986084856E-4	8.984528405650513E-5	1.8441926727387893E-4	9.220963363693946E-5	0.0
20-24	2.7899325049125274E-4	1.4422532440649504E-4	1.0876008069997988E-4	2.67171502589081E-4	1.0876008069997988E-4	0.0
25-29	3.191871933586366E-4	2.1279146223909107E-4	1.2058182860215159E-4	3.168228437782023E-4	2.080627630782224E-4	0.0
30-34	4.4213337154122253E-4	2.7190020174994975E-4	1.3949662524562637E-4	3.5465243706515176E-4	3.2391589251950533E-4	0.0
35-39	7.707779632215965E-4	3.4283068916298007E-4	1.7023316979127286E-4	4.0430377825427305E-4	4.137611765760104E-4	0.0
40-44	0.0010095772708454654	3.8775333119123265E-4	1.98605364756485E-4	4.492264202825256E-4	4.917847127303438E-4	7.093048741303036E-6
45-49	0.0013524079600084455	4.161255261564448E-4	2.1042711265865677E-4	4.917847127303438E-4	6.431030858781418E-4	1.1821747902171727E-5
50-54	0.0017685334861648901	4.3740467238035394E-4	2.2224886056082844E-4	5.177925581151215E-4	7.849640607042026E-4	1.891479664347476E-5
55-59	0.002054619785397446	4.3740467238035394E-4	2.269775597216971E-4	5.225212572759903E-4	8.795380439215765E-4	3.0736544545646495E-5
60-64	0.0022981477921821835	4.492264202825256E-4	2.3879930762386886E-4	5.414360539194651E-4	9.859337750411218E-4	4.4922642028252564E-5
65-69	0.0024400087670082443	4.6104816818469727E-4	2.5534975468690925E-4	6.170952404933642E-4	0.0011207017011258796	5.910873951085863E-5
70-74	0.004489899853244822	5.012421110520812E-4	2.860862992325558E-4	6.691109312629197E-4	0.0012767487734345463	5.910873951085863E-5
75-79	0.040666812783470736	5.390717043390307E-4	3.144584941977679E-4	7.140335732911723E-4	0.0013760514558127889	5.910873951085863E-5
80-84	0.05626915566475697	5.556221514020711E-4	3.712028841281922E-4	7.353127195150813E-4	0.0015462846256040618	5.910873951085863E-5
85-89	0.05642283838748521	5.721725984651116E-4	4.137611765760105E-4	7.967858086063744E-4	0.001924580558473557	5.910873951085863E-5
90-94	0.056500861923639546	5.934517446890206E-4	4.3740467238035383E-4	8.275223531520208E-4	0.001957681452599638	5.910873951085863E-5
95-99	0.05659070720769606	6.147308909129298E-4	4.492264202825256E-4	8.606232472781016E-4	0.00198605364756485	5.910873951085863E-5
100-104	0.056730203832941674	6.265526388151015E-4	4.563194690238286E-4	9.173676372085258E-4	0.002028611940012668	5.910873951085863E-5
105-109	0.056862607409446	6.50196134619445E-4	4.870560135694751E-4	9.812050758802534E-4	0.0020546197853974464	5.910873951085863E-5
110-114	0.05701629013217424	6.620178825216166E-4	5.012421110520812E-4	0.0010119416204258996	0.0020782632812017887	5.910873951085863E-5
115-119	0.05715815110700029	6.620178825216166E-4	5.27249956436859E-4	0.0010639573111954554	0.0021231859232300416	6.383743867172733E-5
120-124	0.05762156362476543	6.620178825216166E-4	5.508934522412025E-4	0.0011017869044824048	0.002215395556866981	7.093048741303036E-5
125-129	0.05843489988043484	6.620178825216166E-4	5.721725984651116E-4	0.001144345196930223	0.0023194269384060927	7.093048741303036E-5
130-134	0.05917257694953036	6.785683295846571E-4	5.863586959477176E-4	0.0011585312944128292	0.002402179173721295	7.093048741303036E-5
135-139	0.0598984322707237	7.329483699346471E-4	5.910873951085863E-4	0.0011608956439932636	0.0025038462056799714	7.093048741303036E-5
140-144	0.06065029543730183	7.471344674172531E-4	6.147308909129298E-4	0.0012341904809867281	0.0025913271401560426	7.093048741303036E-5
145-149	0.06133832116520822	7.660492640607279E-4	6.525604841998792E-4	0.0013098496675606273	0.002652800229247335	7.32948369934647E-5
150-154	0.061967238153603756	7.825997111237682E-4	6.738396304237883E-4	0.0013547723095888799	0.0027331881149821025	8.275223531520208E-5
155-159	0.06264344213360798	7.920571094455056E-4	6.951187766476975E-4	0.0014351601953236474	0.0028324907973603457	8.275223531520208E-5
160-164	0.06332910351193394	8.204293044107178E-4	7.163979228716066E-4	0.0015131837314779808	0.0029010569351929417	8.275223531520208E-5
165-167	0.06399506197708961	8.511658489563642E-4	7.211266220324753E-4	0.001532886644648267	0.0029593775581769886	8.275223531520208E-5
>>END_MODULE
