##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479376
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8453268
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.23117130558265	39.0	39.0	39.0	37.0	39.0
2	39.92807917600625	41.0	40.0	41.0	38.0	41.0
3	39.95746816497478	41.0	40.0	41.0	38.0	41.0
4	39.973832132141084	41.0	40.0	41.0	38.0	41.0
5	39.9799872664631	41.0	40.0	41.0	38.0	41.0
6	39.9611162215607	41.0	40.0	41.0	38.0	41.0
7	39.98824880507752	41.0	40.0	41.0	38.0	41.0
8	39.963708473456656	41.0	40.0	41.0	38.0	41.0
9	39.942158819523996	41.0	40.0	41.0	38.0	41.0
10-14	39.87928256858767	41.0	40.0	41.0	38.0	41.0
15-19	39.743497520722165	41.0	39.8	41.0	37.6	41.0
20-24	39.55993835756775	40.0	39.0	41.0	37.0	41.0
25-29	39.31575518485869	40.0	38.6	41.0	36.0	41.0
30-34	39.23454510137381	40.0	38.4	41.0	35.2	41.0
35-39	39.1030463011465	40.0	38.0	41.0	35.0	41.0
40-44	38.64367286119404	40.0	36.8	41.0	35.0	41.0
45-49	38.07576298302621	39.0	35.4	41.0	34.4	41.0
50-54	37.457614191339964	37.8	35.0	40.2	34.0	41.0
55-59	36.79113654032973	36.4	35.0	39.4	34.0	41.0
60-64	36.05115784806539	35.4	34.8	38.2	33.0	40.0
65-69	35.61375186495921	35.0	35.0	37.0	33.0	39.0
70-74	35.29804260316838	35.0	35.0	36.0	33.0	38.4
75-79	35.055052010654336	35.0	35.0	35.6	33.0	37.0
80-84	34.94340264617187	35.0	34.8	35.0	33.0	37.0
85-89	34.74284643524847	35.0	33.8	35.0	32.4	37.2
90-94	39.43873547291723	40.6	39.8	40.6	36.8	40.6
95-99	39.699842160365876	41.0	39.8	41.0	37.0	41.0
100-104	39.58348903335603	41.0	39.0	41.0	36.4	41.0
105-109	39.43569273521771	40.4	39.0	41.0	36.0	41.0
110-114	39.24944213113675	40.0	38.4	41.0	35.2	41.0
115-119	38.99315603521759	40.0	38.0	41.0	35.0	41.0
120-124	38.7042831287835	40.0	37.8	41.0	34.8	41.0
125-129	38.283029923185765	39.6	36.6	40.6	34.0	41.0
130-134	38.08594294590418	39.0	35.6	41.0	34.0	41.0
135-139	37.77298060572632	39.0	35.0	41.0	34.0	41.0
140-144	37.33846144969474	37.4	35.0	40.4	34.0	41.0
145-149	36.69058941013177	36.2	35.0	39.2	34.0	41.0
150-154	36.03700471962069	35.2	35.0	37.6	33.4	39.8
155-159	35.463282516481556	35.0	35.0	36.4	33.0	38.8
160-164	35.02020430299838	35.0	35.0	35.8	33.0	37.0
165-169	34.72537017196471	35.0	35.0	35.0	33.0	36.0
170-174	34.556498003901936	35.0	34.4	35.0	33.0	36.0
175-178	34.245760318674066	35.0	33.75	35.0	32.25	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	53.0
30	576.0
31	4470.0
32	23561.0
33	89335.0
34	254425.0
35	614445.0
36	1386621.0
37	2362363.0
38	2720415.0
39	980238.0
40	16764.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.750980567515427	28.539696126988996	29.132058749349955	21.57726455614562
2	21.49770952488434	29.287655377778155	29.266255370112482	19.94837972722502
3	19.801596258393793	27.972897582331473	30.493484886555116	21.732021272719614
4	20.343434042313575	27.1086756033288	31.025385685157502	21.522504669200124
5	21.2365679166921	28.03256681321354	29.274287766577377	21.456577503516982
6	21.534571008514103	28.059361184337227	28.80446946671985	21.601598340428815
7	21.406892576930012	28.378835262291457	28.57960968468053	21.634662476098
8	21.511905218194904	28.582602610020174	28.512464055321562	21.393028116463363
9	21.82029482562247	28.434659826235247	28.163297318859403	21.581748029282878
10-14	21.593800172903542	28.568865910793317	28.259619829869347	21.57771408643379
15-19	21.615876841950353	28.48304821283319	28.311221175053248	21.58985377016321
20-24	21.524307522250567	28.418410489292423	28.458572471616893	21.598709516840113
25-29	21.558142957256294	28.44177423453273	28.284237528018753	21.715845280192227
30-34	21.645933856586588	28.45777041494485	28.235801822443108	21.660493906025458
35-39	21.590539895339887	28.468481065547667	28.314259053421708	21.62671998569074
40-44	21.572700640746277	28.488404721109045	28.307147011073113	21.631747627071565
45-49	21.741018976329627	28.403296807814442	28.185541970277058	21.67014224557887
50-54	21.69826154807821	28.458875313074188	28.245521140463076	21.597341998384533
55-59	21.626925823243745	28.458070890453254	28.25268996558491	21.662313320718095
60-64	21.649175206559168	28.462234960491017	28.238397268370058	21.650192564579758
65-69	21.608125993402787	28.4502869186213	28.289033306408832	21.652553781567082
70-74	21.558107468023017	28.493931577704622	28.292915828529274	21.655045125743086
75-79	21.59494056026616	28.457488867027518	28.266589915284833	21.680980657421483
80-84	21.574444345074593	28.51288282827423	28.264479488879328	21.648193337771854
85-89	21.549838476669613	28.504424561010016	28.233509217973452	21.712227744346922
90-94	20.698283529062696	28.22593740967434	29.709186810989884	21.36659225027308
95-99	21.3656907439918	28.462955312026754	28.544206517088693	21.62714742689275
100-104	21.480586906972203	28.546384213735028	28.334563747019605	21.638465132273172
105-109	21.382550972076604	28.521577145635163	28.469396414293712	21.626475467994517
110-114	21.42550827599748	28.34529734419617	28.512922288953195	21.71627209085316
115-119	21.448500473841495	28.428630738101855	28.372035872748114	21.750832915308536
120-124	21.39332255241419	28.465556585570624	28.418014581302174	21.72310628071301
125-129	21.405613521614463	28.458095387788298	28.41434103620657	21.721950054390675
130-134	21.473431450836504	28.466219247463904	28.378595716856264	21.681753584843328
135-139	21.522815886670234	28.468710383065986	28.354174086689277	21.6542996435745
140-144	21.50390475010521	28.50592752022002	28.419354392805474	21.5708133368693
145-149	21.445596769034754	28.564147426055943	28.468317455068163	21.52193834984114
150-154	21.333468810211947	28.674859065060094	28.540925388302075	21.450746736425888
155-159	21.30550510313378	28.68194363612603	28.584346884001395	21.428204376738798
160-164	21.22389801061835	28.81878781685841	28.650932805292562	21.306381367230678
165-169	21.10011632181793	28.90655320022702	28.73364528355938	21.259685194395676
170-174	21.081451495917364	29.00357756994883	28.766535254838228	21.148435679295574
175-178	20.981707502028115	29.134517153776308	28.81412341151077	21.069651932684806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	1.0
7	4.0
8	11.0
9	26.5
10	78.0
11	195.0
12	384.5
13	704.5
14	1295.0
15	2275.5
16	3867.5
17	6228.0
18	9715.0
19	14721.5
20	21382.5
21	29690.0
22	39764.0
23	51289.0
24	63754.0
25	77341.5
26	91800.5
27	107082.0
28	122744.0
29	138712.5
30	155094.5
31	171716.0
32	188113.5
33	204253.0
34	221133.5
35	237591.0
36	254281.5
37	271141.5
38	287699.5
39	303131.0
40	315070.5
41	323851.5
42	329670.0
43	329754.5
44	324054.0
45	315399.0
46	302179.5
47	284920.0
48	265398.5
49	245280.0
50	225694.0
51	206696.0
52	189274.5
53	173573.5
54	160197.5
55	148953.0
56	138462.5
57	129618.0
58	121301.0
59	112829.0
60	105497.0
61	97829.0
62	89701.5
63	81059.5
64	71598.0
65	61711.0
66	52069.5
67	42951.5
68	34599.0
69	27335.5
70	20963.5
71	15593.0
72	11246.5
73	7830.5
74	5416.0
75	3673.5
76	2317.0
77	1392.0
78	847.5
79	525.0
80	312.5
81	171.5
82	88.0
83	45.0
84	19.5
85	6.5
86	4.5
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1556.0
92-93	2979.0
94-95	4339.0
96-97	5530.0
98-99	6858.0
100-101	8239.0
102-103	9504.0
104-105	10785.0
106-107	11981.0
108-109	12723.0
110-111	13760.0
112-113	14361.0
114-115	15293.0
116-117	15975.0
118-119	16876.0
120-121	17447.0
122-123	18158.0
124-125	19064.0
126-127	19785.0
128-129	20976.0
130-131	21641.0
132-133	23698.0
134-135	103233.0
136-137	84892.0
138-139	80083.0
140-141	80650.0
142-143	79893.0
144-145	84229.0
146-147	85049.0
148-149	89533.0
150-151	88130.0
152-153	84266.0
154-155	84762.0
156-157	87120.0
158-159	87825.0
160-161	89769.0
162-163	88750.0
164-165	91490.0
166-167	94021.0
168-169	96779.0
170-171	97397.0
172-173	101460.0
174-175	108283.0
176-177	263570.0
178-179	6010556.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.21056610918367
#Duplication Level	Percentage of total
1	92.87475143555865
2	6.0745683738309
3	0.6927859899773359
4	0.15870909298578423
5	0.06345012069821743
6	0.03294193901620111
7	0.02174561821375393
8	0.00937439229692519
9	0.010477161338933696
>10	0.05959228730585927
>50	0.0016035887774235255
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	2.1293539965845163E-4	2.365948885093907E-5	0.0	2.365948885093907E-5	0.0	0.0
3	2.2476514408392115E-4	3.5489233276408605E-5	0.0	4.731897770187814E-5	2.365948885093907E-5	0.0
4	2.2476514408392115E-4	4.731897770187814E-5	0.0	4.731897770187814E-5	2.365948885093907E-5	0.0
5	2.2476514408392115E-4	5.9148722127347675E-5	1.1829744425469535E-5	7.097846655281721E-5	2.365948885093907E-5	0.0
6	2.2476514408392115E-4	7.097846655281721E-5	1.1829744425469535E-5	7.097846655281721E-5	2.365948885093907E-5	0.0
7	2.2476514408392115E-4	8.280821097828675E-5	1.1829744425469535E-5	7.097846655281721E-5	2.365948885093907E-5	0.0
8	2.365948885093907E-4	8.280821097828675E-5	1.1829744425469535E-5	7.097846655281721E-5	2.365948885093907E-5	0.0
9	2.365948885093907E-4	8.280821097828675E-5	2.365948885093907E-5	7.097846655281721E-5	2.365948885093907E-5	0.0
10-14	2.578884284752359E-4	8.280821097828675E-5	2.365948885093907E-5	8.044226209319284E-5	4.2587079931690326E-5	0.0
15-19	2.933776617516444E-4	1.1829744425469534E-4	3.075733550622079E-5	1.0883364871431972E-4	9.227200651866239E-5	0.0
20-24	3.075733550622079E-4	1.5378667753110394E-4	6.151467101244158E-5	1.4195693310563442E-4	1.348590864503527E-4	0.0
25-29	3.3359879279824085E-4	2.03471604118076E-4	9.463795540375628E-5	1.5615262641619784E-4	2.1293539965845163E-4	0.0
30-34	4.353345948572789E-4	2.2476514408392113E-4	9.700390428885019E-5	2.082035018882638E-4	2.3896083739448462E-4	0.0
35-39	6.648316367113878E-4	2.6498627513051753E-4	1.2302934202488316E-4	2.8391386621126884E-4	3.3359879279824085E-4	0.0
40-44	9.747709406586896E-4	3.4069663945352255E-4	1.3249313756525877E-4	3.4779448610880437E-4	4.258707993169032E-4	4.731897770187814E-6
45-49	0.0013012718868016487	3.690880260746495E-4	1.4905477976091616E-4	3.8564966827030683E-4	5.205087547206595E-4	1.8927591080751256E-5
50-54	0.0016987512994974252	4.211389015467155E-4	1.7271426861185523E-4	4.1877295266162155E-4	6.530018922859184E-4	2.365948885093907E-5
55-59	0.0019471759324322852	4.921173680995327E-4	1.7981211526713693E-4	4.56628134823124E-4	7.783971831958954E-4	2.365948885093907E-5
60-64	0.0021553794343205494	5.228747036057535E-4	1.9164185969260645E-4	4.944833169846266E-4	8.706691897145578E-4	2.365948885093907E-5
65-69	0.002306800162966559	5.34704448031223E-4	2.1530134854354552E-4	5.418022946865047E-4	9.41647656267375E-4	2.365948885093907E-5
70-74	0.004303661021985816	5.725596301927254E-4	2.578884284752358E-4	5.772915279629133E-4	0.0010244558672456616	2.365948885093907E-5
75-79	0.04095457520097553	6.10414812354228E-4	2.6025437736032974E-4	6.293424034349792E-4	0.00111436192487923	2.365948885093907E-5
80-84	0.05683955601549602	6.317083523200731E-4	2.6025437736032974E-4	6.62465687826294E-4	0.001296539989031461	2.365948885093907E-5
85-89	0.056964951306406	6.813932789070452E-4	2.768160195559871E-4	6.813932789070451E-4	0.0016963853506123314	2.365948885093907E-5
90-94	0.057040661670729	7.310782054940171E-4	3.1467120171748965E-4	7.09784665528172E-4	0.0017295086350036458	2.365948885093907E-5
95-99	0.057114006086166916	7.452738988045806E-4	3.2650094614295914E-4	7.287122566089234E-4	0.0017484362260843972	2.365948885093907E-5
100-104	0.057258328968157635	7.760312343108014E-4	3.5016043499389824E-4	7.618355410002381E-4	0.0018028530504415568	2.365948885093907E-5
105-109	0.057433409185654594	8.233502120126796E-4	3.8091777050011904E-4	8.067885698170221E-4	0.0018383422837179655	2.365948885093907E-5
110-114	0.057563536374334756	8.87230831910215E-4	3.9984536158087024E-4	8.517415986338065E-4	0.0018501720281434355	2.365948885093907E-5
115-119	0.057712591154095674	9.156222185313419E-4	4.400664926274667E-4	9.156222185313419E-4	0.00188329531253475	2.365948885093907E-5
120-124	0.058076947282400135	9.321838607269994E-4	4.684578792485936E-4	0.0010007963783947224	0.0019211504946962522	2.365948885093907E-5
125-129	0.05880329359012396	9.605752473481261E-4	4.8265357255915703E-4	0.0010694088960624458	0.0019992268079043513	2.365948885093907E-5
130-134	0.05951071230676704	0.0010504813049816947	5.134109080653777E-4	0.001140387362615263	0.0020702052744571682	2.365948885093907E-5
135-139	0.06028437759219275	0.0011072640782239485	5.465341924566925E-4	0.0012113658291680804	0.002181404872056582	2.365948885093907E-5
140-144	0.06098469846218054	0.0011356554648450754	5.701936813076316E-4	0.0012823442957208976	0.0022784087763454324	2.365948885093907E-5
145-149	0.06194527370952867	0.0011758765958916717	6.151467101244158E-4	0.0013107356823420242	0.0023517531917833434	2.365948885093907E-5
150-154	0.062633764835091	0.0011877063403171414	6.198786078946035E-4	0.0013296632734227758	0.0024511230449572874	2.365948885093907E-5
155-159	0.06333645165396388	0.001194804186972423	6.62465687826294E-4	0.0013864460466650294	0.0025552247959014195	2.365948885093907E-5
160-164	0.06411484883715979	0.0012373912669041132	6.813932789070451E-4	0.0014408628710221893	0.002673522240156115	2.365948885093907E-5
165-167	0.06470870200731836	0.001242123164674301	6.86125176677233E-4	0.001502377542034631	0.002701124977148877	2.365948885093907E-5
>>END_MODULE
