##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479377
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6569950
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.16263989832495	39.0	39.0	39.0	37.0	39.0
2	39.86261310968881	41.0	40.0	41.0	38.0	41.0
3	39.91425688171143	41.0	40.0	41.0	38.0	41.0
4	39.92885151332963	41.0	40.0	41.0	38.0	41.0
5	39.9412140122832	41.0	40.0	41.0	38.0	41.0
6	39.92962077336966	41.0	40.0	41.0	38.0	41.0
7	39.95173159613087	41.0	40.0	41.0	38.0	41.0
8	39.93878477005152	41.0	40.0	41.0	38.0	41.0
9	39.92342148722593	41.0	40.0	41.0	38.0	41.0
10-14	39.853451730987295	41.0	40.0	41.0	38.0	41.0
15-19	39.725412522165314	41.0	39.8	41.0	37.4	41.0
20-24	39.5593734807723	40.2	39.0	41.0	37.0	41.0
25-29	39.34305551792632	40.0	38.8	41.0	36.0	41.0
30-34	39.23756905303693	40.0	38.4	41.0	35.4	41.0
35-39	38.86250772075891	40.0	37.8	41.0	35.0	41.0
40-44	38.60526402788454	40.0	36.8	41.0	35.0	41.0
45-49	38.07254746230945	39.0	35.4	41.0	34.4	41.0
50-54	37.45755809404942	37.8	35.0	40.2	34.0	41.0
55-59	36.80431348792608	36.4	35.0	39.4	34.0	41.0
60-64	36.05871718962854	35.4	34.8	38.2	33.0	40.2
65-69	35.62734002541876	35.0	35.0	37.0	33.0	39.0
70-74	35.32161398488573	35.0	35.0	36.0	33.0	38.6
75-79	35.08012940737753	35.0	35.0	35.8	33.0	37.0
80-84	34.97480614007717	35.0	35.0	35.0	33.0	37.0
85-89	34.79049542233959	35.0	34.4	35.0	32.6	37.2
90-94	39.399126290195156	40.6	39.8	40.6	36.8	40.6
95-99	39.66631548221902	41.0	39.6	41.0	37.0	41.0
100-104	39.561052830123046	41.0	39.0	41.0	36.2	41.0
105-109	39.417815032375685	40.4	39.0	41.0	36.0	41.0
110-114	39.23655107453479	40.0	38.4	41.0	35.0	41.0
115-119	38.97789971687649	40.0	38.0	41.0	35.0	41.0
120-124	38.69422627731469	40.0	37.6	41.0	34.8	41.0
125-129	38.23262165854199	39.6	36.6	40.6	34.0	41.0
130-134	38.05226694563889	39.0	35.6	41.0	34.0	41.0
135-139	37.708997880487594	39.0	35.0	41.0	34.0	41.0
140-144	37.3422248199641	37.4	35.0	40.4	34.0	41.0
145-149	36.705503870806275	36.2	35.0	39.2	34.0	41.0
150-154	36.05822269801566	35.2	35.0	37.6	33.8	40.0
155-159	35.483316724334685	35.0	35.0	36.4	33.0	38.8
160-164	35.0357803494352	35.0	35.0	35.8	33.0	37.0
165-169	34.74995876504339	35.0	35.0	35.0	33.0	36.0
170-174	34.587190855413134	35.0	35.0	35.0	33.0	36.0
175-178	34.29110272086326	35.0	34.0	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	47.0
30	496.0
31	3553.0
32	18968.0
33	72161.0
34	205677.0
35	486098.0
36	1077442.0
37	1825306.0
38	2093102.0
39	771922.0
40	15176.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.715774092649106	28.530156241676117	29.214073166462455	21.53999649921232
2	21.503177345337484	29.272429774960234	29.283708399607306	19.940684480094976
3	19.766649670088814	28.02143090891103	30.542256790386535	21.669662630613626
4	20.326867023341123	27.11917137877762	31.063874154293416	21.490087443587853
5	21.226447689860652	28.024231539052806	29.305885128501743	21.4434356425848
6	21.494958104703993	28.04083744929566	28.874953386251036	21.589251059749312
7	21.37644883142185	28.430429455323097	28.613216234522334	21.579905478732712
8	21.48296410170549	28.623444622866234	28.523124224689685	21.37046705073859
9	21.75112443778111	28.506822730766597	28.188631572538604	21.55342125891369
10-14	21.567935829039794	28.59773970882579	28.285323328183626	21.54900113395079
15-19	21.605318153106186	28.496131629616663	28.331518504707038	21.567031712570113
20-24	21.508793826437035	28.429278761634414	28.485735812296898	21.576191599631656
25-29	21.543205047222582	28.463799572295073	28.312300702440655	21.68069467804169
30-34	21.58133623543558	28.480493763270648	28.277140617508508	21.661029383785266
35-39	21.55285808872214	28.50076789016659	28.30573139826026	21.64064262285101
40-44	21.539436373183968	28.525115107420906	28.305089079825567	21.630359439569556
45-49	21.718274872715927	28.41607318168327	28.198768635986575	21.666883309614228
50-54	21.67378442758316	28.47091073752464	28.245904458938043	21.609400375954156
55-59	21.615309096720676	28.46016484143715	28.258300291478626	21.66622577036355
60-64	21.633768902350855	28.464177048531567	28.262059833027646	21.639994216089924
65-69	21.600709290025037	28.4715926300809	28.29055624472028	21.637141835173786
70-74	21.548386212984877	28.524658482941273	28.269670241021622	21.657285063052232
75-79	21.582255572721255	28.491903286935212	28.252458542302456	21.67338259804108
80-84	21.545125914200263	28.537809267954856	28.262604738240015	21.654460079604867
85-89	21.538456152634343	28.522189666588027	28.213191881216755	21.72616229956088
90-94	20.680134744928306	28.2512579051446	29.706292302663083	21.36231504726401
95-99	21.362123542356485	28.48443307720665	28.547901972950612	21.605541407486257
100-104	21.466299564230635	28.558258247694756	28.359515853380984	21.615926334693622
105-109	21.364855575457582	28.541568377115915	28.495898040897732	21.597678006528767
110-114	21.42016583834939	28.364605920690305	28.50771781332615	21.70751042763415
115-119	21.450021280126787	28.460068791923675	28.370954006833156	21.718955921116383
120-124	21.383168676618514	28.477123145657586	28.42699789943793	21.71271027828597
125-129	21.412415404011302	28.465573078006194	28.422949377199146	21.699062140783358
130-134	21.471758536270546	28.482361442335392	28.386642690241693	21.659237331152372
135-139	21.51357080289145	28.486022677961014	28.359308078600154	21.64109844054738
140-144	21.495438910127774	28.53675754617841	28.418605839772876	21.549197703920942
145-149	21.4122339929492	28.605498241919836	28.47686724471391	21.505400520417055
150-154	21.31265345927505	28.715149594897504	28.538730748237345	21.433466197590096
155-159	21.302763540206147	28.6889125216228	28.58564264181669	21.422681296354362
160-164	21.213619288298954	28.850094295503204	28.641341903380514	21.29494451281733
165-169	21.100695304701205	28.92352036981339	28.726117922638732	21.249666402846675
170-174	21.078183893807008	29.028022325971868	28.744321774436767	21.149472005784357
175-178	20.98316165811786	29.127271647041024	28.81365572890018	21.075910965940935
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	3.0
7	6.0
8	14.0
9	36.0
10	83.5
11	165.0
12	316.5
13	603.5
14	1079.5
15	1887.5
16	3109.5
17	4958.0
18	7737.5
19	11492.5
20	16637.0
21	23302.0
22	31104.0
23	39865.0
24	49782.0
25	60620.5
26	71966.0
27	83849.0
28	96102.5
29	108516.0
30	121175.0
31	134471.0
32	147001.0
33	159258.5
34	172478.0
35	185532.5
36	198104.0
37	210876.0
38	223456.0
39	234997.5
40	244495.5
41	251237.0
42	255437.0
43	255621.0
44	252038.0
45	244697.0
46	233723.0
47	220488.5
48	205727.0
49	190267.0
50	174614.5
51	160011.5
52	146762.0
53	135093.5
54	125020.5
55	115788.5
56	107596.5
57	100670.0
58	94196.5
59	87922.5
60	81959.0
61	75768.5
62	69131.5
63	62534.5
64	55426.5
65	47836.0
66	40300.5
67	33203.0
68	26927.5
69	21268.0
70	16300.0
71	11984.5
72	8632.0
73	6093.0
74	4185.5
75	2792.5
76	1779.5
77	1101.5
78	655.0
79	384.5
80	219.0
81	125.5
82	71.0
83	28.0
84	11.5
85	6.0
86	3.5
87	2.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1246.0
92-93	2537.0
94-95	3844.0
96-97	4884.0
98-99	6007.0
100-101	7046.0
102-103	7957.0
104-105	8891.0
106-107	10045.0
108-109	10705.0
110-111	11361.0
112-113	11735.0
114-115	12695.0
116-117	13412.0
118-119	14028.0
120-121	15749.0
122-123	15759.0
124-125	16973.0
126-127	17662.0
128-129	17386.0
130-131	18499.0
132-133	22140.0
134-135	83240.0
136-137	72738.0
138-139	75091.0
140-141	69770.0
142-143	70787.0
144-145	67067.0
146-147	71033.0
148-149	74867.0
150-151	67416.0
152-153	63912.0
154-155	64402.0
156-157	67903.0
158-159	74187.0
160-161	71428.0
162-163	71369.0
164-165	73221.0
166-167	71311.0
168-169	72729.0
170-171	77313.0
172-173	80884.0
174-175	84603.0
176-177	210347.0
178-179	4583771.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.95554948708711
#Duplication Level	Percentage of total
1	94.23181404093106
2	5.028484429513822
3	0.47493422422895504
4	0.12986582440966518
5	0.03709271928043327
6	0.018687240191275985
7	0.02301300382251523
8	0.00737765108111749
9	0.008495148602681616
>10	0.038544875483929146
>50	0.001690842454550147
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.5220815987945114E-5	0.0	0.0	0.0	0.0
2	2.1309142383123158E-4	1.5220815987945114E-5	0.0	1.5220815987945114E-5	0.0	0.0
3	2.1309142383123158E-4	3.0441631975890228E-5	0.0	3.0441631975890228E-5	0.0	0.0
4	2.1309142383123158E-4	3.0441631975890228E-5	0.0	3.0441631975890228E-5	1.5220815987945114E-5	0.0
5	2.1309142383123158E-4	3.0441631975890228E-5	0.0	6.0883263951780456E-5	1.5220815987945114E-5	0.0
6	2.2831223981917672E-4	4.566244796383534E-5	0.0	6.0883263951780456E-5	1.5220815987945114E-5	0.0
7	2.2831223981917672E-4	4.566244796383534E-5	1.5220815987945114E-5	6.0883263951780456E-5	1.5220815987945114E-5	0.0
8	2.2831223981917672E-4	4.566244796383534E-5	3.0441631975890228E-5	7.610407993972556E-5	1.5220815987945114E-5	0.0
9	2.2831223981917672E-4	4.566244796383534E-5	3.0441631975890228E-5	9.132489592767068E-5	1.5220815987945114E-5	0.0
10-14	2.2831223981917672E-4	4.566244796383534E-5	3.348579517347925E-5	1.4003150708909504E-4	2.435330558071218E-5	0.0
15-19	2.4962138220229987E-4	6.69715903469585E-5	7.610407993972558E-5	2.2526807662158767E-4	1.0654571191561579E-4	0.0
20-24	2.64842198190245E-4	7.914824313731458E-5	9.132489592767068E-5	2.800630141781901E-4	1.491639966818621E-4	0.0
25-29	3.1659297254925834E-4	1.1567820150838286E-4	9.132489592767068E-5	2.9528383016613513E-4	1.8264979185534137E-4	0.0
30-34	4.231386844648742E-4	1.5829648627462917E-4	9.43690591252597E-5	3.7747623650103877E-4	2.039589342384645E-4	0.0
35-39	8.067032473610909E-4	2.2222391342399862E-4	1.0958987511320481E-4	4.5053615324317544E-4	3.287696253396144E-4	0.0
40-44	0.0012724602165922114	2.67886361387834E-4	1.4003150708909504E-4	4.809777852190656E-4	4.535803164407644E-4	0.0
45-49	0.00161949482111736	3.135488093516693E-4	1.9787060784328645E-4	5.32728559578079E-4	5.631701915539692E-4	1.5220815987945112E-5
50-54	0.0019543527728521524	3.5312293092032665E-4	2.1613558702882063E-4	5.783910075419143E-4	7.153783514334204E-4	1.5220815987945112E-5
55-59	0.0022070183182520417	3.6529958371068274E-4	2.58753871795067E-4	5.966559867274484E-4	8.371448793369812E-4	1.5220815987945112E-5
60-64	0.0024931696588254095	3.896528892913949E-4	2.7397468778301204E-4	6.149209659129825E-4	9.467347544501861E-4	1.5220815987945112E-5
65-69	0.0027641001834108328	4.2618284766246316E-4	2.7397468778301204E-4	6.69715903469585E-4	0.0010806779351441032	1.5220815987945112E-5
70-74	0.004648437202718438	4.4140366365040827E-4	3.0137215656131323E-4	7.366874938165435E-4	0.0011598261782814177	1.5220815987945112E-5
75-79	0.0383534121264241	4.7184529562629854E-4	3.1659297254925834E-4	7.884382681755569E-4	0.0011994002998500749	1.5220815987945112E-5
80-84	0.05264271417590697	4.840219484166546E-4	3.348579517347925E-4	8.493215321273374E-4	0.001363785112519882	1.5220815987945112E-5
85-89	0.05276752486700812	5.327285595780791E-4	3.348579517347925E-4	8.767190009056387E-4	0.0016164506579197714	1.5220815987945112E-5
90-94	0.05284971727334302	5.723026811467362E-4	3.348579517347925E-4	9.041164696839398E-4	0.0016286273107101272	1.5220815987945112E-5
95-99	0.05296235131165382	5.966559867274483E-4	3.348579517347925E-4	9.893530392164323E-4	0.001643848126698072	1.5220815987945112E-5
100-104	0.05311760363473086	6.301417819009277E-4	3.4703460452514855E-4	0.0010197946711923227	0.0016682014322787846	1.5220815987945112E-5
105-109	0.053236325999436826	6.636275770744071E-4	3.896528892913949E-4	0.001041103813575446	0.0017016872274522635	1.5220815987945112E-5
110-114	0.05333982754815485	6.879808826551192E-4	4.292270108600522E-4	0.0010563246295633908	0.0017625704914040444	1.5220815987945112E-5
115-119	0.05340375497530423	7.153783514334204E-4	4.5662447963835343E-4	0.0010958987511320482	0.0018264979185534137	1.5220815987945112E-5
120-124	0.05373556876384143	7.153783514334204E-4	4.901102748118327E-4	0.0011841794838621299	0.001856939550529304	1.5220815987945112E-5
125-129	0.05435353389295201	7.427758202117216E-4	5.083752539973668E-4	0.0012359302582211434	0.002018280200001522	1.5220815987945112E-5
130-134	0.05511153052915167	7.458199834093106E-4	5.235960699853119E-4	0.0012785485429873897	0.0021217817487195487	2.435330558071218E-5
135-139	0.05603086781482356	7.732174521876117E-4	5.692585179491472E-4	0.0013120343381608688	0.002273989908599	3.0441631975890225E-5
140-144	0.05683452689898705	7.97570757768324E-4	5.936118235298594E-4	0.0014003150708909507	0.002414021415688095	3.0441631975890225E-5
145-149	0.05758339104559396	8.493215321273373E-4	6.270976187033386E-4	0.0014672866612379088	0.002484037169232643	3.0441631975890225E-5
150-154	0.05818917952191417	8.554098585225152E-4	6.423184346912838E-4	0.001482507477225854	0.0025570970859747793	4.261828476624632E-5
155-159	0.05904154521723909	8.736748377080496E-4	6.544950874816399E-4	0.0015312140883872783	0.002630157002716916	4.566244796383534E-5
160-164	0.0598178068326243	8.919398168935838E-4	6.69715903469585E-4	0.0015890531891414698	0.002712349409051819	4.566244796383534E-5
165-167	0.06030994654956786	8.980281432887618E-4	6.849367194575301E-4	0.0016286273107101272	0.0027600412991473804	4.566244796383534E-5
>>END_MODULE
