##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479387
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11476866
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.925581600412514	39.0	37.0	39.0	35.0	39.0
2	39.51917213288018	40.0	39.0	41.0	37.0	41.0
3	39.54519334807952	40.0	39.0	41.0	37.0	41.0
4	39.543499331611955	40.0	39.0	41.0	37.0	41.0
5	39.527151314653324	40.0	39.0	41.0	37.0	41.0
6	39.48949443166802	40.0	39.0	41.0	37.0	41.0
7	39.52869189202	40.0	39.0	41.0	37.0	41.0
8	39.496258124822575	40.0	39.0	41.0	37.0	41.0
9	39.45115034017126	40.0	39.0	41.0	37.0	41.0
10-14	39.287904624833985	40.0	38.8	41.0	36.6	41.0
15-19	39.15242514811971	40.0	38.2	41.0	36.0	41.0
20-24	39.28231860509655	40.0	39.0	41.0	36.4	41.0
25-29	39.15641738781301	40.0	38.4	41.0	36.0	41.0
30-34	38.858967683337944	40.0	38.0	41.0	35.0	41.0
35-39	38.487788321306525	40.0	37.6	40.4	34.4	41.0
40-44	37.897158701687374	39.0	36.4	40.0	33.4	41.0
45-49	37.194949962820864	38.2	35.0	40.0	32.6	41.0
50-54	36.56866487767654	37.2	34.6	39.6	32.0	40.8
55-59	36.003689474112534	36.0	34.0	39.0	31.2	40.0
60-64	35.42914604039117	35.2	33.6	38.6	30.8	40.0
65-69	35.390092364936564	35.0	33.8	38.0	31.0	40.0
70-74	35.40701607912822	35.0	34.0	38.0	31.0	40.6
75-79	35.506736647443645	35.0	34.0	38.8	31.0	41.0
80-84	35.74419931364538	35.0	34.0	39.6	31.0	41.0
85-89	35.967732410572715	35.4	33.8	40.0	30.6	41.0
90-94	39.05492980809194	39.8	38.0	40.8	35.8	41.0
95-99	39.00270592488939	40.0	38.0	41.0	35.0	41.0
100-104	38.779016452198434	40.0	38.0	41.0	35.0	41.0
105-109	38.61679344439334	40.0	37.8	41.0	34.2	41.0
110-114	38.44263944442042	40.0	37.0	41.0	34.0	41.0
115-119	38.167159215719906	39.8	36.2	41.0	34.0	41.0
120-124	37.84617943741662	39.0	35.4	41.0	33.0	41.0
125-129	37.34582373330334	38.6	35.0	40.4	33.0	41.0
130-134	37.1637375038064	38.0	35.0	40.0	32.8	41.0
135-139	36.72029116174521	37.0	35.0	40.0	32.0	41.0
140-144	36.171992367338675	36.0	34.2	39.0	32.0	41.0
145-149	35.5711124640092	35.0	34.0	37.8	31.6	39.8
150-154	34.96345217603773	35.0	34.0	36.6	31.0	39.0
155-159	34.42010157197182	35.0	34.0	35.6	31.0	37.2
160-164	34.01445724474537	35.0	33.4	35.0	31.0	36.2
165-169	33.723302967161764	34.8	33.0	35.0	30.4	35.8
170-174	33.487905645174855	34.0	33.0	35.0	30.0	35.0
175-178	32.966676194902305	33.75	32.0	34.75	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	70.0
31	1297.0
32	12896.0
33	79183.0
34	331555.0
35	1053859.0
36	2652266.0
37	4330726.0
38	2718535.0
39	294264.0
40	2215.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.000997484853443	28.40370358946423	27.670384929126122	21.924913996556203
2	21.98411134189421	28.490643700118135	28.3237688755798	21.201476082407865
3	21.016277440200138	27.95641249100582	28.88088960871374	22.146420460080304
4	21.330509565938993	27.448739054721038	29.092236504286102	22.12851487505387
5	21.79674311785116	28.03894373254859	28.2293092905328	21.935003859067447
6	21.869916404007853	28.009310207159345	28.13037113093418	21.990402257898626
7	21.77335694256603	28.172900162814486	28.033741964051863	22.02000093056763
8	21.74640707663573	28.401333604487494	28.021073000242403	21.831186318634373
9	21.808044112390963	28.32167771236503	27.982935411113104	21.887342764130903
10-14	21.74668938366972	28.303075072933677	27.962252064283057	21.98798347911355
15-19	21.78294318326972	28.262157979364748	27.939418304613824	22.01548053275171
20-24	21.78097748984784	28.244569554092553	27.994259059921063	21.980193896138545
25-29	21.728726291654883	28.259470834633778	27.955762487773228	22.05604038593811
30-34	21.714919386529388	28.21707075781838	28.021130507230808	22.046879348421424
35-39	21.70245431113337	28.20338060930571	28.01042897947924	22.083736100081676
40-44	21.849008257132216	28.104844998625932	27.93941133406977	22.106735410172078
45-49	22.279467234347774	27.898705099458336	27.667973120885094	22.1538545453088
50-54	22.14324886253791	27.962198042566673	27.757431340576773	22.137121754318645
55-59	22.050650412752052	27.9663089209197	27.74675943763742	22.236281228690828
60-64	21.900703554437246	27.987356478676322	27.829567758306144	22.282372208580288
65-69	21.810396671007574	27.996107996730117	27.929276163022205	22.264219169240103
70-74	21.702950962396876	27.985688776012545	27.976712457913163	22.334647803677417
75-79	21.644215415602133	27.948798914268057	28.01718692193496	22.38979874819485
80-84	21.568644262292512	27.97013574960272	28.068153797386845	22.39306619071792
85-89	21.546068412753097	27.883303682381584	28.131467248985913	22.4391606558794
90-94	21.628310771924458	28.077711883718433	28.176455320905188	22.117522023451926
95-99	21.714061055917043	28.161535610640243	27.988314382896817	22.136088950545897
100-104	21.75819994206451	28.13685312470671	27.96901855145209	22.135928381776683
105-109	21.76174033297395	28.149240896372575	27.98741313824121	22.10160563241227
110-114	21.757262735028647	28.142210113012922	28.006363692483482	22.09416345947495
115-119	21.739419792872983	28.200794685036957	28.032819725688206	22.026965796401857
120-124	21.709315044675566	28.213361305120806	28.07508819997535	22.002235450228273
125-129	21.700540556137	28.226233456805172	28.125897661475356	21.947328325582472
130-134	21.733549389742425	28.253075337914414	28.15763441789596	21.855740854447202
135-139	21.723456954918632	28.299313089818632	28.192434090264424	21.784795864998312
140-144	21.656035114734994	28.381543252877577	28.298032611215852	21.664389021171576
145-149	21.545253984457386	28.489607514352972	28.41151620156886	21.553622299620784
150-154	21.424200058934666	28.60563608151582	28.552975458651552	21.417188400897963
155-159	21.31267788342543	28.704971064269465	28.680613564522655	21.30173748778245
160-164	21.154214004916604	28.83057323042994	28.857568840533453	21.157643924120002
165-169	20.99749386795443	28.96248888285743	29.018685217494227	21.021332031693916
170-174	20.83728072911604	29.129594307542668	29.257254693402636	20.775870269938657
175-178	20.661853189925434	29.246081759991295	29.561389134378206	20.530675915705064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.5
4	2.0
5	2.0
6	3.5
7	5.0
8	9.0
9	15.0
10	25.5
11	41.5
12	71.0
13	120.5
14	174.5
15	287.5
16	459.0
17	753.0
18	1294.0
19	2199.5
20	3473.0
21	5412.0
22	8474.5
23	12928.0
24	19203.5
25	27924.5
26	39128.0
27	53244.0
28	70679.5
29	91797.0
30	116487.5
31	144508.0
32	176208.5
33	210795.5
34	248851.0
35	291244.0
36	335294.0
37	379399.0
38	422927.0
39	463491.0
40	499639.0
41	531137.5
42	555782.0
43	570670.0
44	576880.5
45	573916.5
46	561296.0
47	539385.0
48	509834.0
49	474862.0
50	433608.0
51	389972.0
52	346831.0
53	303284.5
54	260964.0
55	222275.5
56	187945.0
57	157170.0
58	130927.0
59	109095.5
60	90383.5
61	74400.0
62	60580.0
63	48693.5
64	38621.0
65	30057.5
66	22821.5
67	16866.0
68	12157.5
69	8586.5
70	5857.0
71	3878.0
72	2499.0
73	1547.0
74	902.5
75	509.5
76	274.0
77	149.5
78	84.5
79	43.0
80	23.5
81	11.5
82	4.0
83	2.0
84	1.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	5714.0
92-93	13000.0
94-95	20851.0
96-97	28558.0
98-99	36366.0
100-101	44041.0
102-103	51706.0
104-105	59644.0
106-107	67488.0
108-109	75669.0
110-111	82500.0
112-113	89748.0
114-115	96413.0
116-117	103481.0
118-119	112153.0
120-121	120047.0
122-123	127210.0
124-125	135354.0
126-127	143597.0
128-129	153005.0
130-131	161695.0
132-133	171331.0
134-135	278750.0
136-137	280452.0
138-139	285462.0
140-141	288308.0
142-143	289214.0
144-145	291876.0
146-147	295998.0
148-149	300248.0
150-151	306745.0
152-153	279892.0
154-155	286054.0
156-157	286777.0
158-159	299917.0
160-161	308711.0
162-163	311020.0
164-165	318929.0
166-167	326671.0
168-169	339503.0
170-171	338456.0
172-173	356064.0
174-175	363320.0
176-177	509166.0
178-179	2635762.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.27731094163451
#Duplication Level	Percentage of total
1	87.86544145728574
2	9.29724135564625
3	1.6159022159538534
4	0.48499827576168836
5	0.19729981141132996
6	0.12053809916414195
7	0.10055019799912396
8	0.07729115074929649
9	0.04968422676837099
>10	0.1898156595846348
>50	0.00123754967556258
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	1.7426360123051014E-5	0.0
2	0.0	0.0	0.0	1.7426360123051014E-5	1.7426360123051014E-5	0.0
3	0.0	8.713180061525507E-6	8.713180061525507E-6	2.613954018457652E-5	3.485272024610203E-5	0.0
4	1.7426360123051014E-5	8.713180061525507E-6	8.713180061525507E-6	4.3565900307627536E-5	3.485272024610203E-5	0.0
5	2.613954018457652E-5	8.713180061525507E-6	8.713180061525507E-6	6.099226043067855E-5	4.3565900307627536E-5	0.0
6	2.613954018457652E-5	8.713180061525507E-6	8.713180061525507E-6	6.970544049220406E-5	4.3565900307627536E-5	0.0
7	2.613954018457652E-5	1.7426360123051014E-5	8.713180061525507E-6	6.970544049220406E-5	5.227908036915304E-5	0.0
8	2.613954018457652E-5	2.613954018457652E-5	8.713180061525507E-6	6.970544049220406E-5	7.841862055372956E-5	0.0
9	2.613954018457652E-5	4.3565900307627536E-5	8.713180061525507E-6	6.970544049220406E-5	8.713180061525507E-5	0.0
10-14	3.485272024610203E-5	4.879380834454284E-5	1.0455816073830608E-5	8.538916460294997E-5	1.0455816073830609E-4	0.0
15-19	5.9249624418373454E-5	6.099226043067856E-5	3.659535625840713E-5	1.2546979288596732E-4	1.5335196908284894E-4	0.0
20-24	6.099226043067856E-5	6.447753245528875E-5	5.053644435684794E-5	1.6380778515667955E-4	1.8297678129203563E-4	0.0
25-29	6.447753245528875E-5	9.235970865217037E-5	7.144807650450916E-5	2.1260159350122238E-4	2.23057409575053E-4	0.0
30-34	2.6488067387037535E-4	1.132713407998316E-4	9.410234466447547E-5	2.5791012982115494E-4	3.032186661410876E-4	0.0
35-39	8.521490100171947E-4	1.4463878902132342E-4	1.1849924883674691E-4	3.3458611436257946E-4	4.1997527896552945E-4	6.970544049220406E-6
40-44	0.001360998725610284	1.7426360123051014E-4	1.4115351699671322E-4	3.676961985963764E-4	5.489303438761068E-4	8.713180061525507E-6
45-49	0.0016920995679482538	1.9866050540278155E-4	1.6729305718128973E-4	4.0080628283017326E-4	6.081799682944803E-4	8.713180061525507E-6
50-54	0.0019517523337817138	2.3177058963657848E-4	1.7426360123051014E-4	4.1997527896552945E-4	6.622016846759385E-4	1.7426360123051014E-5
55-59	0.002251485727898191	2.666233098826805E-4	1.7426360123051014E-4	4.600559072485468E-4	7.632745733896345E-4	1.7426360123051014E-5
60-64	0.002558189666063889	2.75336489944206E-4	1.7949150926742545E-4	5.140776236300049E-4	8.660900981156353E-4	1.7426360123051014E-5
65-69	0.002885805236377248	2.7882176196881623E-4	1.9517523337817135E-4	5.489303438761069E-4	0.0010246699752353996	1.7426360123051014E-5
70-74	0.0064163857973073835	3.0496130215339277E-4	2.126015935012224E-4	5.907536081714294E-4	0.0011902203964043842	1.7426360123051014E-5
75-79	0.059335013582976395	3.3807138638718967E-4	2.23057409575053E-4	6.935691328974305E-4	0.0013313739134010977	4.008062828301733E-5
80-84	0.07813631351973616	3.642109265717662E-4	2.3525586166118869E-4	7.58046665352719E-4	0.0014568437062870648	5.9249624418373454E-5
85-89	0.0782931507608436	4.0254891884247847E-4	2.4396904172271423E-4	7.772156614880752E-4	0.0016415631235914055	6.099226043067856E-5
90-94	0.07842036318974187	4.321737310516652E-4	2.596527658334601E-4	8.068404736972619E-4	0.0017077832920589994	6.099226043067856E-5
95-99	0.07863122214723078	4.7051172332237737E-4	2.631380378580703E-4	8.399505579310589E-4	0.0017461212843297117	6.099226043067856E-5
100-104	0.078906558637175	4.7051172332237737E-4	2.735938539319009E-4	8.887443662756017E-4	0.0017931724566619494	6.099226043067856E-5
105-109	0.0791801524911069	4.8271017540851304E-4	2.7882176196881623E-4	9.305676305709241E-4	0.0018332530849449666	6.099226043067856E-5
110-114	0.0794885990652849	4.931659914823438E-4	3.223876622764438E-4	9.741335308785517E-4	0.001894245345375645	6.099226043067856E-5
115-119	0.07974825183111836	5.105923516053946E-4	3.5201247448563044E-4	0.0010211847032107894	0.0019534949697940186	6.447753245528875E-5
120-124	0.08165121035655552	5.297613477407509E-4	3.711814706209866E-4	0.0010473242433953658	0.002075479490655376	6.970544049220406E-5
125-129	0.08592241122271532	5.437024358391916E-4	3.746667426455968E-4	0.001103088595789129	0.0021765523793690717	7.493334852911937E-5
130-134	0.09039401523029021	5.489303438761069E-4	3.746667426455968E-4	0.0011396839520475364	0.0022497430918858863	7.841862055372956E-5
135-139	0.09553827673861488	5.541582519130223E-4	3.78152014670207E-4	0.001171051400269028	0.00231596326035348	7.841862055372956E-5
140-144	0.10017717380337106	5.66356703999158E-4	3.833799227071223E-4	0.0012233304806381813	0.002392639244894904	8.016125656603466E-5
145-149	0.10520119342684667	5.66356703999158E-4	3.833799227071223E-4	0.0012459847487981475	0.002467572593424024	9.235970865217037E-5
150-154	0.11115229540886859	5.733272480483783E-4	3.833799227071223E-4	0.0012581832008842831	0.002502425313670126	9.584498067678057E-5
155-159	0.11843825657631621	5.83783064122209E-4	3.833799227071223E-4	0.0012773521970196394	0.0025250795818300914	9.584498067678057E-5
160-164	0.12579218054824376	5.83783064122209E-4	3.833799227071223E-4	0.0012982638291673006	0.0025320501258793127	9.758761668908567E-5
165-167	0.128504883940732	5.866874574760509E-4	3.8337992270712234E-4	0.0012982638291673006	0.0025471529713192898	1.045581607383061E-4
>>END_MODULE
