##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479388
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11311297
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.92933604342632	39.0	37.0	39.0	35.0	39.0
2	39.54890336625411	40.0	39.0	41.0	37.0	41.0
3	39.571223087856325	40.0	39.0	41.0	37.0	41.0
4	39.56594915684735	40.0	39.0	41.0	37.0	41.0
5	39.555558306001515	40.0	39.0	41.0	37.0	41.0
6	39.52206586035182	40.0	39.0	41.0	37.0	41.0
7	39.53629208038654	40.0	39.0	41.0	37.0	41.0
8	39.51089278267558	40.0	39.0	41.0	37.0	41.0
9	39.46177967035964	40.0	39.0	41.0	37.0	41.0
10-14	39.30709475668441	40.0	39.0	41.0	36.6	41.0
15-19	39.17233321696	40.0	38.4	41.0	36.0	41.0
20-24	39.29448173803588	40.0	39.0	41.0	36.6	41.0
25-29	39.1789197825855	40.0	38.6	41.0	36.0	41.0
30-34	38.88972002061302	40.0	38.0	41.0	35.0	41.0
35-39	38.50794661301883	40.0	37.6	40.4	34.4	41.0
40-44	37.92361047543884	39.2	36.4	40.0	33.4	41.0
45-49	37.21321127011341	38.2	35.0	40.0	32.6	41.0
50-54	36.582411972738406	37.2	34.6	39.8	32.0	41.0
55-59	36.02661866274044	36.0	34.0	39.0	31.2	40.0
60-64	35.45824495634762	35.4	33.8	38.8	30.8	40.0
65-69	35.433232811409695	35.0	33.8	38.0	31.0	40.0
70-74	35.438954737020865	35.0	34.0	38.0	31.0	40.8
75-79	35.54576515849597	35.0	34.0	39.0	31.0	41.0
80-84	35.7873285441979	35.0	34.0	39.8	31.0	41.0
85-89	36.01103776162893	35.4	33.8	40.0	30.6	41.0
90-94	39.08281757247426	39.8	38.0	40.8	35.8	41.0
95-99	39.02738254761578	40.0	38.0	41.0	35.0	41.0
100-104	38.80372907178554	40.0	38.0	41.0	35.0	41.0
105-109	38.647197994000116	40.0	37.8	41.0	34.2	41.0
110-114	38.46556806186985	40.0	37.0	41.0	34.0	41.0
115-119	38.185734774086484	39.8	36.2	41.0	34.0	41.0
120-124	37.862951942711284	39.0	35.4	41.0	33.0	41.0
125-129	37.35912289527711	38.6	35.0	40.4	33.0	41.0
130-134	37.17653256531992	38.0	35.0	40.0	33.0	41.0
135-139	36.73558095777051	37.0	35.0	40.0	32.0	41.0
140-144	36.17906141108699	36.0	34.2	39.0	32.0	41.0
145-149	35.5794639989512	35.0	34.0	37.8	31.6	39.8
150-154	34.98075206804296	35.0	34.0	36.6	31.0	39.0
155-159	34.44512783093037	35.0	34.0	35.8	31.0	37.2
160-164	34.0394055367279	35.0	33.6	35.0	31.0	36.2
165-169	33.74795327318534	35.0	33.0	35.0	30.6	35.8
170-174	33.5330563433204	34.2	33.0	35.0	30.0	35.0
175-178	32.96668158425006	33.75	32.0	34.75	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	78.0
31	1235.0
32	12363.0
33	74960.0
34	312816.0
35	1002370.0
36	2563559.0
37	4278812.0
38	2752824.0
39	309723.0
40	2555.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.037083810990023	28.37337751806888	27.644628197809677	21.94491047313142
2	21.986576782485688	28.51047939064813	28.29319219537777	21.209751631488412
3	21.025290026422258	27.951427674474466	28.871189572690025	22.15209272641325
4	21.3520518469279	27.46671756563372	29.059364279799215	22.121866307639166
5	21.82459712621815	28.02267503010486	28.188173292594122	21.96455455108287
6	21.87112583110496	28.06549947366779	28.095876184667418	21.967498510559842
7	21.81893022524296	28.094673846863007	28.01856409570008	22.067831832193956
8	21.78914584242638	28.358286410479717	28.000873816680794	21.851693930413106
9	21.804068976351694	28.331074676935806	27.98174250044005	21.883113846272447
10-14	21.767196104920593	28.2848518609316	27.945836803683967	22.002115230463843
15-19	21.765979621965545	28.25901397514361	27.93798801322253	22.03701838966831
20-24	21.795039065811817	28.221237582215373	27.99127810011531	21.9924452518575
25-29	21.74817441359731	28.218829370318893	27.960549528493505	22.07244668759029
30-34	21.730675094111664	28.2042315748583	28.0002496619088	22.064843669121235
35-39	21.713628419446508	28.20785626970983	27.991935849620077	22.08657946122359
40-44	21.855242595079947	28.104787629570684	27.920152746409187	22.119817028940183
45-49	22.29880092442096	27.873990047295194	27.663176026586516	22.16403300169733
50-54	22.162965042824002	27.930409748767094	27.742767252950745	22.16385795545816
55-59	22.066235198315454	27.925374075139214	27.756942462035962	22.251448264509367
60-64	21.9218927767523	27.95959649896913	27.821315274455266	22.297195449823302
65-69	21.83091647226662	28.007575081796542	27.899157806571605	22.26235063936523
70-74	21.723273644039228	27.97269490846187	27.9594179164423	22.344613531056606
75-79	21.671610249470067	27.941655143525978	27.993507729484957	22.393226877518998
80-84	21.584718357231715	27.952854566545287	28.05520710843328	22.407219967789725
85-89	21.58592246317995	27.88385628986667	28.083960663396958	22.44626058355642
90-94	21.647806392146503	28.062258332717622	28.15669417133688	22.13324110379899
95-99	21.742853311599728	28.13312394201994	27.97575890138704	22.148263844993295
100-104	21.776779042483348	28.14946607485025	27.91536576598813	22.158389116678272
105-109	21.76555235247499	28.138189740703318	27.972641207503912	22.123616699317783
110-114	21.779595409451616	28.12712657317342	27.97392601328917	22.11935200408579
115-119	21.74052144888781	28.181269394725465	28.012079812146197	22.066129344240522
120-124	21.725756331118617	28.18834859036724	28.059648072183872	22.026247006330273
125-129	21.727064145904993	28.217726322006666	28.09632306800907	21.958886464079274
130-134	21.76443080068346	28.241389158655593	28.12081410416406	21.87336593649689
135-139	21.758559027651838	28.27107715747912	28.16431634714846	21.806047467720582
140-144	21.675126468471372	28.349573266869	28.29804305578702	21.677257208872604
145-149	21.562217809857795	28.460337627610915	28.40607802467626	21.571366537855038
150-154	21.43688770351063	28.587261463433673	28.545080762690638	21.430770070365053
155-159	21.326638937147806	28.662092270383056	28.679841757770173	21.33142703469896
160-164	21.169351831926562	28.838747054046415	28.82777090792083	21.164130206106194
165-169	21.019740682477664	28.938121237030035	29.01410652080954	21.028031559682763
170-174	20.8418321401056	29.10005649555157	29.269779995774677	20.78833136856816
175-178	20.70013633000962	29.198618837133665	29.54825728455561	20.5529875483011
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.5
4	3.5
5	3.0
6	3.0
7	6.0
8	12.0
9	19.0
10	28.5
11	44.5
12	74.5
13	108.0
14	172.5
15	288.5
16	483.0
17	790.5
18	1307.0
19	2172.5
20	3493.0
21	5429.0
22	8399.0
23	12817.0
24	19056.5
25	27502.5
26	38476.0
27	52416.5
28	69588.0
29	89998.5
30	114095.0
31	142097.5
32	173444.5
33	207916.0
34	245371.0
35	285771.5
36	328779.5
37	372979.0
38	415229.0
39	455665.0
40	492217.0
41	522262.0
42	545386.5
43	559702.0
44	565904.0
45	563086.5
46	551065.5
47	531612.0
48	503868.0
49	468932.5
50	428673.0
51	385836.0
52	342127.0
53	299071.5
54	258500.5
55	220330.5
56	186448.5
57	156726.5
58	130891.0
59	108843.5
60	90349.0
61	74471.0
62	60703.0
63	48872.5
64	38624.5
65	30113.0
66	22787.0
67	16990.0
68	12421.0
69	8675.5
70	5925.0
71	3883.0
72	2521.0
73	1579.0
74	921.0
75	538.5
76	316.5
77	173.0
78	94.0
79	52.0
80	27.0
81	12.0
82	3.0
83	1.5
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	5444.0
92-93	12850.0
94-95	20104.0
96-97	28256.0
98-99	36247.0
100-101	43651.0
102-103	52078.0
104-105	59761.0
106-107	68294.0
108-109	75666.0
110-111	81510.0
112-113	88097.0
114-115	96030.0
116-117	102982.0
118-119	110825.0
120-121	117968.0
122-123	125763.0
124-125	134196.0
126-127	141545.0
128-129	150269.0
130-131	159625.0
132-133	168896.0
134-135	274443.0
136-137	279875.0
138-139	285195.0
140-141	283931.0
142-143	286419.0
144-145	288816.0
146-147	292117.0
148-149	298121.0
150-151	304972.0
152-153	274922.0
154-155	273961.0
156-157	285700.0
158-159	293472.0
160-161	305655.0
162-163	303687.0
164-165	316416.0
166-167	321711.0
168-169	329395.0
170-171	328306.0
172-173	347101.0
174-175	365873.0
176-177	503970.0
178-179	2587182.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.3123977049611
#Duplication Level	Percentage of total
1	87.90620352848985
2	9.365016725420672
3	1.4991599991086773
4	0.4597762766060455
5	0.22176751398384603
6	0.12875370871969252
7	0.09237969410591293
8	0.06907973469444932
9	0.05178270707939348
>10	0.2060801117914221
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.840719150067406E-6	8.840719150067406E-6	0.0	0.0	8.840719150067406E-6	0.0
2	8.840719150067406E-6	8.840719150067406E-6	0.0	0.0	8.840719150067406E-6	0.0
3	1.7681438300134812E-5	8.840719150067406E-6	0.0	8.840719150067406E-6	1.7681438300134812E-5	0.0
4	1.7681438300134812E-5	2.6522157450202217E-5	0.0	1.7681438300134812E-5	1.7681438300134812E-5	0.0
5	1.7681438300134812E-5	2.6522157450202217E-5	0.0	1.7681438300134812E-5	2.6522157450202217E-5	0.0
6	1.7681438300134812E-5	3.5362876600269625E-5	0.0	1.7681438300134812E-5	3.5362876600269625E-5	0.0
7	2.6522157450202217E-5	3.5362876600269625E-5	0.0	2.6522157450202217E-5	4.420359575033703E-5	0.0
8	2.6522157450202217E-5	4.420359575033703E-5	0.0	3.5362876600269625E-5	5.3044314900404434E-5	0.0
9	4.420359575033703E-5	5.3044314900404434E-5	8.840719150067406E-6	3.5362876600269625E-5	5.3044314900404434E-5	0.0
10-14	5.127617107039096E-5	8.310276001063363E-5	1.768143830013481E-5	6.718946554051229E-5	8.310276001063361E-5	0.0
15-19	7.779832852059316E-5	9.194347916070102E-5	3.889916426029659E-5	1.0608862980080887E-4	1.113930612908493E-4	0.0
20-24	7.956647235060666E-5	1.0962491746083582E-4	6.011689022045835E-5	1.2907449959098416E-4	1.5382851321117286E-4	0.0
25-29	1.0255234214078192E-4	1.2553821193095715E-4	6.895760937052577E-5	1.43219650231092E-4	1.9980025279152337E-4	0.0
30-34	2.7406229365208956E-4	1.5029222555114588E-4	8.663904767066059E-5	1.6620552002126726E-4	2.5107642386191435E-4	1.7681438300134812E-6
35-39	9.052896409669024E-4	1.6797366385128072E-4	9.724791065074147E-5	2.1217725960161773E-4	3.553969098327097E-4	8.840719150067406E-6
40-44	0.0013738477559204752	1.9980025279152337E-4	1.0255234214078192E-4	2.581489991819682E-4	4.3319523835330285E-4	1.768143830013481E-5
45-49	0.0017875934121436294	2.3693127322180647E-4	1.1492934895087628E-4	2.8467115663217046E-4	5.569653064542466E-4	3.5362876600269625E-5
50-54	0.002091714150905948	2.510764238619143E-4	1.1846563661090323E-4	3.3948361536258843E-4	6.789672307251767E-4	4.066730809031007E-5
55-59	0.0024205889032884557	2.758304374821031E-4	1.3791521874105154E-4	3.836872111129254E-4	7.832877166959722E-4	4.420359575033703E-5
60-64	0.002710564491410667	2.9704816344226483E-4	1.4498779406110546E-4	4.4557224516339727E-4	8.982170656468485E-4	4.420359575033703E-5
65-69	0.0029846267850627563	3.200340332324401E-4	1.5913294470121331E-4	5.092254230438827E-4	9.77783537997455E-4	4.420359575033703E-5
70-74	0.006388303657838707	3.3064289621252094E-4	1.609010885312268E-4	5.711104570943544E-4	0.001090944743118318	4.420359575033703E-5
75-79	0.06013103537109847	3.447880468526288E-4	1.7327809534132117E-4	6.259229158247724E-4	0.0012058740920691943	4.420359575033703E-5
80-84	0.0794550792893158	3.713102043028311E-4	1.9272767747146945E-4	6.895760937052577E-4	0.001288976852079828	4.420359575033703E-5
85-89	0.07966902469274743	3.74846491962858E-4	1.9449582130148295E-4	7.267071141355408E-4	0.0014304283584809063	4.420359575033703E-5
90-94	0.07981578063063854	3.8899164260296584E-4	2.0156839662153683E-4	7.709107098858778E-4	0.001492313392531378	4.420359575033703E-5
95-99	0.07996253656852967	4.172819438831816E-4	2.157135472616447E-4	8.062735864861473E-4	0.00155419842658185	4.420359575033703E-5
100-104	0.08012520580089091	4.4203595750337034E-4	2.2809055407173908E-4	8.610860452165654E-4	0.001605474597652241	4.420359575033703E-5
105-109	0.08028257060176212	4.6855811495357254E-4	2.4046756088183345E-4	9.123622162869563E-4	0.0016620552002126724	4.420359575033703E-5
110-114	0.08052657445030398	4.7209440261359947E-4	2.5991714301198173E-4	9.388843737371585E-4	0.0017257083780931575	5.127617107039096E-5
115-119	0.08077057829884585	4.827032655936804E-4	2.722941498220761E-4	9.795516818274686E-4	0.0017928978436336698	5.3044314900404434E-5
120-124	0.08247860523863886	4.880076970837208E-4	2.7406229365208956E-4	0.0010414367158779404	0.0018883776104543979	6.188503405047184E-5
125-129	0.08667794683492087	5.039209915538421E-4	2.8113486897214353E-4	0.00107679959247821	0.001987393664935153	6.188503405047184E-5
130-134	0.0915332697921379	5.039209915538421E-4	2.917437319522244E-4	0.0011086261814184527	0.002082873431755881	6.188503405047184E-5
135-139	0.09677758439195787	5.039209915538421E-4	2.970481634422649E-4	0.0011457572018487357	0.0021412221781463257	6.188503405047184E-5
140-144	0.10154803644533425	5.21602429853977E-4	3.0588888259233226E-4	0.0011581342086588301	0.0021801213424066223	6.895760937052577E-5
145-149	0.10632202478637065	5.21602429853977E-4	3.1826588940242663E-4	0.0011634386401488705	0.0022526152394371753	7.072575320053925E-5
150-154	0.11225945176755592	5.21602429853977E-4	3.1826588940242663E-4	0.0012023378044091673	0.002298586979017526	7.072575320053925E-5
155-159	0.11968211956595251	5.21602429853977E-4	3.1826588940242663E-4	0.001230628105689383	0.0023463268624278897	7.072575320053925E-5
160-164	0.12656196720853496	5.21602429853977E-4	3.1826588940242663E-4	0.0012536139754795582	0.0023640083007280244	7.072575320053925E-5
165-167	0.12904503052715058	5.21602429853977E-4	3.1826588940242663E-4	0.0012642228384596391	0.002395834889668267	7.072575320053925E-5
>>END_MODULE
