##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479395
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8980248
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-180
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.39711408860869	40.0	39.0	41.0	36.0	41.0
2	39.50520419926042	40.0	39.0	41.0	37.0	41.0
3	39.5891465358195	40.0	39.0	41.0	37.0	41.0
4	39.58467694878805	40.0	39.0	41.0	37.0	41.0
5	39.60328222561337	40.0	39.0	41.0	37.0	41.0
6	39.56500210239183	40.0	39.0	41.0	37.0	41.0
7	39.51332992140083	40.0	39.0	41.0	37.0	41.0
8	39.50533971890309	40.0	39.0	41.0	37.0	41.0
9	39.43522985111324	40.0	39.0	41.0	37.0	41.0
10-14	39.277975864363654	40.0	38.4	41.0	36.0	41.0
15-19	39.1255836809852	40.0	38.0	41.0	36.0	41.0
20-24	39.07567606150743	40.0	38.0	41.0	35.6	41.0
25-29	38.78303850851335	40.0	38.0	41.0	35.0	41.0
30-34	38.3924235054533	39.8	37.4	40.6	34.2	41.0
35-39	38.12647450270862	39.0	36.6	40.0	34.0	41.0
40-44	37.64359859549536	38.8	35.4	40.0	33.4	41.0
45-49	36.944727695716196	37.4	35.0	40.0	33.0	41.0
50-54	36.35200471078304	36.4	34.6	39.0	32.4	40.6
55-59	35.74900151977986	35.4	34.0	38.4	32.0	40.0
60-64	35.16838265491109	35.0	33.8	37.0	31.4	39.4
65-69	35.22741930957808	35.0	34.0	36.6	32.0	39.0
70-74	35.01568257357703	35.0	34.0	36.0	32.0	39.0
75-79	34.94658457093835	35.0	34.0	36.0	31.8	40.0
80-84	34.97081942503147	35.0	34.0	36.0	31.0	40.0
85-89	35.51525675014766	35.6	34.4	37.2	31.4	40.2
90-94	39.135248277504466	40.0	38.0	41.0	35.6	41.0
95-99	39.01017461043584	40.0	38.0	41.0	35.0	41.0
100-104	38.928407648929266	40.0	38.0	41.0	35.0	41.0
105-109	38.73528748496594	40.0	38.0	41.0	34.4	41.0
110-114	38.49584704794565	40.0	37.4	41.0	34.0	41.0
115-119	38.2200960002692	39.8	36.8	41.0	34.0	41.0
120-124	37.90888988277605	39.0	35.8	40.6	33.2	41.0
125-129	37.21473447563293	38.2	35.0	39.8	32.8	40.6
130-134	37.23735119824604	38.0	35.0	40.0	33.0	41.0
135-139	36.82967885963522	37.4	35.0	40.0	32.8	41.0
140-144	36.41718961511236	36.4	35.0	39.2	32.6	41.0
145-149	35.792078045846594	35.4	34.0	38.2	32.0	40.0
150-154	35.157726083605496	35.0	34.0	36.8	32.0	39.0
155-159	34.61107908723575	35.0	34.0	35.8	31.0	37.6
160-164	34.2467642108178	35.0	34.0	35.0	31.0	36.4
165-169	33.93819221192076	35.0	33.6	35.0	31.0	36.0
170-174	33.70802762811059	34.4	33.0	35.0	31.0	35.2
175-179	33.50830445499796	34.0	33.0	35.0	30.6	35.0
180	31.42754100663312	32.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	145.0
31	2475.0
32	21329.0
33	113636.0
34	426197.0
35	1178109.0
36	2357520.0
37	3014646.0
38	1675150.0
39	190369.0
40	671.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.934717170394403	28.47367912333824	28.301323081500644	21.290280624766712
2	21.20106259871665	28.374572728949133	29.078139044712355	21.346225627621866
3	21.010399712791898	28.219398840655625	29.16339281498685	21.606808631565634
4	21.288699376676455	28.56974551259609	28.85606277243123	21.285492338296226
5	21.156576076740865	28.515515384430362	29.002272543029996	21.32563599579878
6	21.016000894407373	28.293105045651302	29.048373719745825	21.642520340195503
7	21.323219581463672	28.45459279075589	28.748226106895935	21.4739615208845
8	21.259724675755056	28.485248959716923	28.841085457773545	21.41394090675447
9	21.093782710677925	28.226937607959158	28.87030514079344	21.80897454056948
10-14	21.339769235771662	28.396128926506258	28.676755920326475	21.587345917395602
15-19	21.351662003098358	28.380185046114537	28.56479910131658	21.703353849470528
20-24	21.342074294607453	28.382009049193297	28.563696681873374	21.712219974325876
25-29	21.408005658641052	28.40913079460612	28.468260564741644	21.71460298201119
30-34	21.376340608856236	28.362626510982768	28.420506872415995	21.840526007744998
35-39	21.439182971338877	28.31399088310256	28.44348619325435	21.803339952304214
40-44	21.581893952149205	28.33020869802259	28.29887548762573	21.78902186220247
45-49	21.786044216150824	28.193822709573276	28.174645065481485	21.845488008794412
50-54	21.70802409911174	28.19133948193858	28.24946037125033	21.85117604769935
55-59	21.66318123953815	28.266569030164867	28.255460205553344	21.814789524743638
60-64	21.549351421029797	28.238516352777786	28.334789863264355	21.87734236292806
65-69	21.51562629450768	28.268894132990535	28.355201326288537	21.860278246213245
70-74	21.594800054519652	28.307926462609938	28.258845412732477	21.83842807013793
75-79	21.548467258365246	28.299577027271404	28.28850606352965	21.863449650833697
80-84	21.554910287555533	28.310745983852563	28.28536138422903	21.84898234436287
85-89	21.53859670690609	28.38124737757799	28.2127999137663	21.86735600174962
90-94	21.244051351216996	28.41776532082679	28.73165971860582	21.6065236093504
95-99	21.24032721600453	28.485854791388544	28.662004287316112	21.61181370529081
100-104	21.364644347391696	28.385200214495377	28.542787417535976	21.707368020576954
105-109	21.444210182035622	28.46209832980678	28.40607906908284	21.68761241907476
110-114	21.473991537337824	28.439327329671404	28.34612764721592	21.740553485774857
115-119	21.448507574337455	28.467090929614834	28.36924242341326	21.715159072634446
120-124	21.463277474723025	28.517669978456368	28.326688077293582	21.692364469527032
125-129	21.452089038925006	28.52969207315534	28.318833003354538	21.699385884565118
130-134	21.553933442690848	28.507419879639002	28.285878223943623	21.652768453726534
135-139	21.57623481378691	28.57522513148747	28.30884523141597	21.539694823309652
140-144	21.516074690582123	28.612763577589266	28.3735197157298	21.497642016098812
145-149	21.416051210238447	28.722534155565782	28.454250024405077	21.407164609790698
150-154	21.354557232586917	28.8728019924789	28.506980566112073	21.26566020882211
155-159	21.250704060597137	28.96408715272661	28.58068615324127	21.204522633434987
160-164	21.174991308088178	29.073071213777123	28.68112375595212	21.070813722182578
165-169	21.099897234872156	29.314281585819863	28.747305608733143	20.83851557057484
170-174	21.022944321190344	29.416691464242255	28.85917120089381	20.701193013673585
175-179	20.88261686256446	29.691699713255332	28.996160041394937	20.429523382785266
180	20.915880945298035	29.414804355236463	29.510529798790998	20.158784900674505
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.5
7	6.0
8	11.0
9	19.0
10	33.0
11	60.0
12	114.0
13	260.5
14	524.5
15	988.0
16	1832.5
17	3199.0
18	5331.0
19	8415.5
20	12569.5
21	18130.0
22	25189.0
23	34196.5
24	45082.5
25	57711.5
26	71877.5
27	87180.0
28	103884.0
29	121836.5
30	140956.0
31	161323.5
32	181993.0
33	203752.0
34	227242.5
35	250906.0
36	274827.0
37	298722.0
38	321652.0
39	343769.5
40	363275.0
41	379277.5
42	389938.0
43	394062.5
44	392176.0
45	383339.0
46	370121.5
47	352153.0
48	329659.5
49	304551.5
50	277072.5
51	249641.5
52	223710.0
53	199465.5
54	177342.0
55	157702.5
56	140747.5
57	125867.5
58	112539.5
59	100675.5
60	90397.5
61	80992.5
62	71726.0
63	62757.0
64	53611.5
65	45250.0
66	37626.5
67	29978.0
68	23418.0
69	18034.0
70	13507.5
71	9741.0
72	6699.5
73	4548.0
74	2984.5
75	1889.0
76	1176.5
77	693.0
78	391.0
79	220.5
80	112.0
81	56.5
82	26.5
83	13.0
84	9.0
85	4.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	3503.0
92-93	7786.0
94-95	12056.0
96-97	15754.0
98-99	19833.0
100-101	23619.0
102-103	27310.0
104-105	30712.0
106-107	33398.0
108-109	35527.0
110-111	38098.0
112-113	40751.0
114-115	43389.0
116-117	46273.0
118-119	49053.0
120-121	51591.0
122-123	55059.0
124-125	57805.0
126-127	61008.0
128-129	64908.0
130-131	68447.0
132-133	93719.0
134-135	129675.0
136-137	150008.0
138-139	158939.0
140-141	152025.0
142-143	158820.0
144-145	162021.0
146-147	168881.0
148-149	164358.0
150-151	165306.0
152-153	152713.0
154-155	153044.0
156-157	157613.0
158-159	160603.0
160-161	162618.0
162-163	167143.0
164-165	179970.0
166-167	183976.0
168-169	192658.0
170-171	203015.0
172-173	215288.0
174-175	227393.0
176-177	244607.0
178-179	787249.0
180-181	3502726.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.77552070842086
#Duplication Level	Percentage of total
1	81.45349427661849
2	14.020744565435775
3	2.8513896618528833
4	0.9412341516371807
5	0.3828870675440957
6	0.18070451714985017
7	0.07743368713955913
8	0.035298426986966565
9	0.012527397703413155
>10	0.032625685526428555
>50	0.001630964073734968
>100	0.01002959833160477
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.113554993136047E-5	0.0	3.340664979408141E-5	0.0	2.227109986272094E-5	0.0
2	1.113554993136047E-5	0.0	3.340664979408141E-5	2.227109986272094E-5	3.340664979408141E-5	0.0
3	3.340664979408141E-5	0.0	3.340664979408141E-5	2.227109986272094E-5	3.340664979408141E-5	0.0
4	4.454219972544188E-5	0.0	3.340664979408141E-5	2.227109986272094E-5	3.340664979408141E-5	0.0
5	6.681329958816282E-5	0.0	5.567774965680235E-5	2.227109986272094E-5	3.340664979408141E-5	0.0
6	6.681329958816282E-5	0.0	6.681329958816282E-5	2.227109986272094E-5	5.567774965680235E-5	0.0
7	6.681329958816282E-5	1.113554993136047E-5	6.681329958816282E-5	2.227109986272094E-5	6.681329958816282E-5	0.0
8	8.908439945088376E-5	1.113554993136047E-5	6.681329958816282E-5	4.454219972544188E-5	6.681329958816282E-5	0.0
9	1.2249104924496516E-4	1.113554993136047E-5	7.79488495195233E-5	6.681329958816282E-5	7.79488495195233E-5	0.0
10-14	1.42535039121414E-4	1.3362659917632565E-5	9.353861942342795E-5	6.904040957443492E-5	1.0021994938224424E-4	0.0
15-19	1.7816879890176753E-4	4.454219972544188E-5	1.2694526921750937E-4	1.0467416935478842E-4	1.2694526921750934E-4	0.0
20-24	2.0489411873703266E-4	1.0244705936851633E-4	1.558976990390466E-4	1.1358260929987679E-4	1.6926035895667915E-4	0.0
25-29	2.8284296825655593E-4	1.2026393925869308E-4	1.7594168891549544E-4	1.2694526921750937E-4	2.85070078242828E-4	0.0
30-34	0.0011180092131085912	1.8707723884685588E-4	1.89304348833128E-4	1.492163690802303E-4	4.74374427075956E-4	0.0
35-39	0.00374377188692339	2.0934833870957684E-4	1.9153145881940008E-4	2.0266700875076052E-4	6.948583157168934E-4	1.113554993136047E-5
40-44	0.00671250949862409	2.1825677865466522E-4	2.3607365854484192E-4	2.538905384350187E-4	9.287048642754633E-4	3.56337597803535E-5
45-49	0.00919796424330375	2.472092084762024E-4	2.7838874828401176E-4	2.8061585827028385E-4	0.0011803682927242099	5.567774965680235E-5
50-54	0.011231315660770172	2.7838874828401176E-4	2.8952429821537225E-4	3.251580579957257E-4	0.001443167271104317	7.1267519560707E-5
55-59	0.012832607740899804	2.8952429821537225E-4	3.028869581330048E-4	3.518833778309909E-4	0.0017505084492098661	1.0912838932733261E-4
60-64	0.014574207750164584	3.006598481467327E-4	3.140225080643653E-4	3.9419846757016066E-4	0.001921995918152817	1.3139948919005354E-4
65-69	0.016055235891035524	3.07341178105549E-4	3.3852071791335824E-4	4.6546598713086764E-4	0.002104618937027129	1.5144347906650242E-4
70-74	0.0516511348016224	3.2070383802318154E-4	3.719273677074397E-4	4.966455269386769E-4	0.0023050588357916174	1.558976990390466E-4
75-79	0.27222188073202436	3.31839387954542E-4	3.964255775564328E-4	5.300521767327584E-4	0.0025121800645149218	1.6035191901159077E-4
80-84	0.3149467587086682	3.340664979408141E-4	4.2537800737797E-4	5.70140156485656E-4	0.0028172941326341993	1.7816879890176753E-4
85-89	0.3153454113962109	3.340664979408141E-4	4.5433043719950714E-4	6.102281362385536E-4	0.003144679300616197	1.7816879890176753E-4
90-94	0.3160024088421612	3.407478278996304E-4	5.010997469112211E-4	6.280450161287305E-4	0.0032337637000670804	1.7816879890176753E-4
95-99	0.31645228505938816	3.852900276250723E-4	5.790485964307445E-4	6.569974459502677E-4	0.0032961227796826998	1.7816879890176753E-4
100-104	0.31692888659645035	4.1646956743288154E-4	6.057739162660095E-4	6.681329958816282E-4	0.003385207179133583	1.7816879890176753E-4
105-109	0.317474528543087	4.7660153706222813E-4	6.280450161287304E-4	6.814956557992608E-4	0.0034698373586119226	1.7816879890176753E-4
110-114	0.31827406102815864	5.122352968425816E-4	6.414076760463631E-4	7.082209756345258E-4	0.0035388777681863576	1.7816879890176753E-4
115-119	0.31885310962458946	5.211437367876701E-4	6.770414358267166E-4	7.193565255658864E-4	0.003643551937541146	1.8262301887431173E-4
120-124	0.31994662062784907	5.567774965680235E-4	7.059938656482538E-4	7.527631753599677E-4	0.0038974424759761644	1.9598567879194426E-4
125-129	0.3240890452023151	5.835028164032886E-4	7.327191854835189E-4	7.883969351403213E-4	0.004111245034658285	2.0043989876448843E-4
130-134	0.32956996287853074	5.901841463621049E-4	7.772613852089609E-4	8.240306949206749E-4	0.004333956033285495	2.0266700875076058E-4
135-139	0.33522904935364817	5.990925863071934E-4	8.06213815030498E-4	8.373933548383074E-4	0.0045299417120774396	2.1157544869584893E-4
140-144	0.34004628825395466	6.235907961561864E-4	8.173493649618585E-4	8.797084445774771E-4	0.004654659871308677	2.1157544869584893E-4
145-149	0.34534012869132347	6.302721261150026E-4	8.240306949206749E-4	8.797084445774771E-4	0.004792740690457546	2.1157544869584893E-4
150-154	0.35358711697048906	6.347263460875468E-4	8.440746847971235E-4	8.819355545637492E-4	0.0049330486195926886	2.1157544869584893E-4
155-159	0.3630924223918983	6.347263460875468E-4	8.552102347284841E-4	8.908439945088376E-4	0.00507335654872783	2.204838886409373E-4
160-164	0.37362887973695164	6.480890060051793E-4	8.841626645500214E-4	9.242506443029191E-4	0.005160213838192441	2.227109986272094E-4
165-169	0.38184691558629563	6.569974459502677E-4	9.108879843852865E-4	9.420675241930958E-4	0.00522257291780806	2.227109986272094E-4
>>END_MODULE
