##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479396
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8770637
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-180
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.35000513645702	40.0	39.0	41.0	36.0	41.0
2	39.45516682539706	40.0	39.0	41.0	37.0	41.0
3	39.551972450803746	40.0	39.0	41.0	37.0	41.0
4	39.54844249055114	40.0	39.0	41.0	37.0	41.0
5	39.557488355748845	40.0	39.0	41.0	37.0	41.0
6	39.51904736223834	40.0	39.0	41.0	37.0	41.0
7	39.46953100441849	40.0	39.0	41.0	37.0	41.0
8	39.46065867279651	40.0	39.0	41.0	37.0	41.0
9	39.38506530369459	40.0	39.0	41.0	37.0	41.0
10-14	39.23749672914293	40.0	38.4	41.0	36.0	41.0
15-19	39.07548530397507	40.0	38.0	41.0	36.0	41.0
20-24	39.023153278376476	40.0	38.0	41.0	35.6	41.0
25-29	38.72517656357229	40.0	38.0	41.0	35.0	41.0
30-34	38.33515136927911	39.6	37.2	40.6	34.0	41.0
35-39	38.08260898267709	39.0	36.6	40.0	34.0	41.0
40-44	37.61427472143699	38.6	35.4	40.0	33.4	41.0
45-49	36.89524879435781	37.4	35.0	40.0	33.0	41.0
50-54	36.323102255856675	36.4	34.4	39.0	32.4	40.6
55-59	35.73339993434912	35.4	34.0	38.4	32.0	40.0
60-64	35.160373847418384	35.0	33.8	37.0	31.4	39.6
65-69	35.21997407941977	35.0	34.0	36.8	32.0	39.0
70-74	35.018476081041776	35.0	34.0	36.0	32.0	39.4
75-79	34.967419743856695	35.0	34.0	36.0	31.2	40.0
80-84	35.01668875362189	35.0	34.0	36.2	31.0	40.0
85-89	35.57402067831562	35.6	34.4	37.8	31.4	40.2
90-94	39.0280365208276	40.0	38.0	41.0	35.0	41.0
95-99	38.89547157329452	40.0	38.0	41.0	35.0	41.0
100-104	38.814645567869654	40.0	38.0	41.0	35.0	41.0
105-109	38.621168659636055	40.0	38.0	41.0	34.0	41.0
110-114	38.382293654583535	40.0	37.0	41.0	34.0	41.0
115-119	38.10187614873815	39.4	36.4	41.0	33.6	41.0
120-124	37.79559658379054	39.0	35.6	40.2	33.0	41.0
125-129	37.13095761671489	38.0	35.0	39.6	32.8	40.6
130-134	37.141365967744136	38.0	35.0	40.0	33.0	41.0
135-139	36.733254810259304	37.2	35.0	40.0	32.2	41.0
140-144	36.35070516511174	36.4	34.8	39.2	32.2	40.8
145-149	35.74751783869646	35.4	34.0	38.2	32.0	40.0
150-154	35.11268523607121	35.0	34.0	36.8	31.4	39.0
155-159	34.575736997868184	35.0	34.0	35.8	31.0	37.6
160-164	34.19829044780857	35.0	34.0	35.0	31.0	36.4
165-169	33.87535114250183	35.0	33.2	35.0	31.0	36.0
170-174	33.634262396438906	34.2	33.0	35.0	30.8	35.0
175-179	33.44741084843234	34.0	32.8	35.0	30.0	35.0
180	31.272548407367875	32.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	4.0
30	160.0
31	2476.0
32	21617.0
33	116872.0
34	437002.0
35	1194686.0
36	2348958.0
37	2917682.0
38	1558802.0
39	171700.0
40	678.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.858195704599336	28.548861388289126	28.377528336881348	21.21541457023019
2	21.112411789474354	28.465651924711967	29.161188634303304	21.260747651510375
3	20.954418704137453	28.30942609983745	29.238754266081244	21.497400929943858
4	21.19563265473192	28.663083422560987	28.925584310466846	21.21569961224025
5	21.06856092664649	28.592541225910956	29.06086524844205	21.278032599000507
6	20.975089950707115	28.363755106955175	29.12431559988174	21.53683934245597
7	21.274110420942062	28.532750813880455	28.813004118172948	21.380134647004546
8	21.17159791244353	28.58412678577394	28.927819039825724	21.3164562619568
9	21.003081076095157	28.320953198724336	28.92593776255932	21.75002796262119
10-14	21.256576916819157	28.480861766368854	28.756882766895952	21.505678549916045
15-19	21.271686423688497	28.456199931658325	28.651150423851767	21.62096322080141
20-24	21.27336703137982	28.46585487462313	28.62682835921724	21.63394973477981
25-29	21.318811849127947	28.49972014575452	28.543295087916647	21.638172917200883
30-34	21.29736072761876	28.44065260026153	28.50504245016639	21.75694422195332
35-39	21.35355961032249	28.40438841557346	28.527435350476825	21.714616623627222
40-44	21.49264642921603	28.415167564225953	28.359488598148573	21.732697408409447
45-49	21.731855964395745	28.276972356739883	28.22646747322914	21.764704205635237
50-54	21.624614038866277	28.252217028250058	28.346844134582245	21.776324798301424
55-59	21.576939052431428	28.32167378492577	28.346144071405533	21.755243091237272
60-64	21.464860534075232	28.312782754547932	28.43025426773449	21.79210244364235
65-69	21.428920157110596	28.33201738938688	28.462847111332962	21.77621534216956
70-74	21.49914082637327	28.37511574130819	28.373478460002392	21.752264972316148
75-79	21.45169387354647	28.367348916618027	28.399535860394177	21.781421349441324
80-84	21.45174632127632	28.376440616570953	28.403111427368387	21.76870163478434
85-89	21.423166869179514	28.442203228796263	28.356567487629462	21.778062414394757
90-94	21.15525669261573	28.490724534517774	28.810346380284425	21.54367239258207
95-99	21.154553209180637	28.536176913239785	28.750191732626508	21.55907814495307
100-104	21.26595063692411	28.47251622085197	28.606624910272018	21.654908231951904
105-109	21.351376711842164	28.52837298558157	28.487865148132435	21.632385154443835
110-114	21.373685144422897	28.51541698858699	28.43149504126664	21.67940282572347
115-119	21.35153832981661	28.545890294978776	28.440445872188818	21.662125503015794
120-124	21.347805696960638	28.602899762922444	28.430489416314796	21.61880512380212
125-129	21.356512029829712	28.603662519115414	28.41302386951466	21.62680158154021
130-134	21.441158753885333	28.610476390175304	28.379571222612764	21.568793633326596
135-139	21.462736546383347	28.679954124337776	28.38973269009201	21.467576639186863
140-144	21.39933449829533	28.71490409493006	28.46709383913787	21.41866756763674
145-149	21.28948838821245	28.83064920153857	28.55454139303729	21.325321017211685
150-154	21.22259716774402	28.97461755886622	28.61774339131423	21.185041882075524
155-159	21.125672341479586	29.056243227145252	28.69737619648879	21.120708234886372
160-164	21.041769032212198	29.176207228549263	28.81470881557462	20.96731492366392
165-169	20.957594686247795	29.39658211240564	28.908819002874825	20.73700419847174
170-174	20.878316245517844	29.514612365240968	29.01179021327781	20.595281175963375
175-179	20.72069991480057	29.75209004618463	29.185221017302343	20.341989021712454
180	20.76647013251358	29.49191370613419	29.755849972107217	19.985766189245012
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.5
5	2.5
6	3.5
7	5.0
8	7.0
9	13.5
10	33.0
11	59.0
12	103.5
13	227.0
14	492.0
15	971.5
16	1895.5
17	3311.5
18	5416.5
19	8536.5
20	12593.0
21	18049.5
22	25414.5
23	34556.0
24	45594.0
25	58325.5
26	72621.0
27	87948.5
28	104263.0
29	121888.0
30	140536.0
31	159928.0
32	180933.5
33	203203.5
34	225752.5
35	248869.0
36	272121.5
37	295430.0
38	317807.0
39	338142.0
40	357082.0
41	372749.5
42	381723.5
43	385431.5
44	383569.0
45	375148.5
46	360628.0
47	341542.0
48	319143.5
49	293938.5
50	267698.0
51	241202.0
52	215752.0
53	192008.0
54	170451.0
55	151273.0
56	134475.0
57	119859.5
58	107118.5
59	95996.5
60	85990.5
61	76543.0
62	67489.5
63	58923.0
64	50496.0
65	42314.5
66	34910.5
67	28031.5
68	21816.0
69	16722.0
70	12419.5
71	8884.0
72	6167.0
73	4110.0
74	2656.0
75	1675.5
76	1034.5
77	624.5
78	347.5
79	180.0
80	99.0
81	44.0
82	22.0
83	11.5
84	4.5
85	3.5
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	3759.0
92-93	8758.0
94-95	12940.0
96-97	17027.0
98-99	21281.0
100-101	25924.0
102-103	29400.0
104-105	33216.0
106-107	35409.0
108-109	38275.0
110-111	40641.0
112-113	44000.0
114-115	46475.0
116-117	49533.0
118-119	52772.0
120-121	56038.0
122-123	59624.0
124-125	62602.0
126-127	66614.0
128-129	70507.0
130-131	75558.0
132-133	100769.0
134-135	137331.0
136-137	156172.0
138-139	162297.0
140-141	156726.0
142-143	164476.0
144-145	172021.0
146-147	169821.0
148-149	168470.0
150-151	168171.0
152-153	157899.0
154-155	158214.0
156-157	161643.0
158-159	163767.0
160-161	167878.0
162-163	174492.0
164-165	183648.0
166-167	194239.0
168-169	201978.0
170-171	211824.0
172-173	222488.0
174-175	232379.0
176-177	254205.0
178-179	817652.0
180-181	3061724.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.87512136740358
#Duplication Level	Percentage of total
1	81.70863904264336
2	13.67290053650268
3	2.8571785705246726
4	0.9548184405567388
5	0.4065928219057364
6	0.22603630017008544
7	0.07543144494641403
8	0.03325437478087818
9	0.02299248521470876
>10	0.03244486846615707
>50	0.0
>100	0.009711114288562053
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.1401680402461076E-5	1.1401680402461076E-5	0.0	0.0	1.1401680402461076E-5	0.0
2	2.2803360804922152E-5	3.4205041207383224E-5	0.0	1.1401680402461076E-5	3.4205041207383224E-5	0.0
3	3.4205041207383224E-5	3.4205041207383224E-5	0.0	1.1401680402461076E-5	6.841008241476645E-5	0.0
4	3.4205041207383224E-5	3.4205041207383224E-5	0.0	1.1401680402461076E-5	7.981176281722753E-5	0.0
5	4.5606721609844304E-5	4.5606721609844304E-5	0.0	1.1401680402461076E-5	9.121344321968861E-5	0.0
6	4.5606721609844304E-5	4.5606721609844304E-5	0.0	1.1401680402461076E-5	1.0261512362214967E-4	0.0
7	4.5606721609844304E-5	5.7008402012305376E-5	0.0	1.1401680402461076E-5	1.0261512362214967E-4	0.0
8	4.5606721609844304E-5	5.7008402012305376E-5	0.0	3.4205041207383224E-5	1.1401680402461075E-4	0.0
9	5.7008402012305376E-5	5.7008402012305376E-5	0.0	3.4205041207383224E-5	1.2541848442707183E-4	0.0
10-14	9.349377930018082E-5	8.893310713919638E-5	2.0523024724429935E-5	3.4205041207383224E-5	1.368201648295329E-4	0.0
15-19	1.824268864393772E-4	1.3682016482953292E-4	2.2803360804922152E-5	3.4205041207383224E-5	1.9838923900282267E-4	0.0
20-24	2.6223864925660473E-4	1.7330554211740837E-4	4.5606721609844304E-5	3.876571336836766E-5	2.12071255485776E-4	0.0
25-29	3.488914203153089E-4	1.8926789468085386E-4	5.700840201230537E-5	6.612974633427424E-5	2.7820100182005024E-4	0.0
30-34	0.0010170298918995278	2.3715495237119037E-4	6.841008241476645E-5	1.1857747618559517E-4	5.199166263522251E-4	0.0
35-39	0.0031582654714817178	2.6223864925660473E-4	9.805445146116524E-5	1.505021813124862E-4	7.411092261599699E-4	0.0
40-44	0.0059653591865676345	2.8276167398103464E-4	1.527825173929784E-4	1.8470722251986944E-4	0.0010649169495898646	9.12134432196886E-6
45-49	0.008332348038118553	2.987240265444802E-4	1.9382856684183828E-4	2.3715495237119037E-4	0.0012359421556267804	1.1401680402461076E-5
50-54	0.010450780256895822	3.192470512689101E-4	2.0294991116380712E-4	2.7135999357857357E-4	0.0014616954275955098	1.1401680402461076E-5
55-59	0.01222032105535778	3.329290677518634E-4	2.12071255485776E-4	3.055650347859568E-4	0.0017034110521276847	1.368201648295329E-5
60-64	0.014081075297039428	3.4433074815432447E-4	2.3487461629069818E-4	3.374897399128478E-4	0.0018675952499231242	3.420504120738323E-5
65-69	0.01570923525851087	3.671341089592467E-4	2.5995831317611256E-4	3.7397511720072327E-4	0.00205914348068447	4.5606721609844304E-5
70-74	0.04867149330202584	4.0589982232761424E-4	2.736403296590658E-4	4.127408305690909E-4	0.0023031394412971373	4.5606721609844304E-5
75-79	0.2550373479144103	4.401048635349975E-4	2.96443690463988E-4	4.5606721609844306E-4	0.002474164647334053	5.244772985132095E-5
80-84	0.29482921251899946	4.5150654393745856E-4	3.1012570694694127E-4	5.016739377082873E-4	0.0026839555667393373	6.384941025378203E-5
85-89	0.29522143032484416	5.039542737887796E-4	3.5117175639580114E-4	5.062346098692718E-4	0.002859541444937238	6.841008241476645E-5
90-94	0.295985342911809	5.381593149961628E-4	3.899374697641688E-4	5.176362902717329E-4	0.0029051481665470823	6.841008241476645E-5
95-99	0.2964186067671026	5.814857005255148E-4	3.990588140861376E-4	5.427199871571471E-4	0.0029461942159959417	6.841008241476645E-5
100-104	0.2968929166718449	6.088497334914214E-4	4.401048635349975E-4	5.928873809279759E-4	0.003032846987054646	6.841008241476645E-5
105-109	0.29740827262603614	6.384941025378202E-4	4.8343124906434964E-4	6.179710778133902E-4	0.0031286211024353196	6.841008241476645E-5
110-114	0.2979441516049518	6.384941025378202E-4	5.221969624327173E-4	6.270924221353591E-4	0.0032357968982184527	6.841008241476645E-5
115-119	0.29873770856096316	6.498957829402813E-4	5.51841331479116E-4	6.567367911817579E-4	0.003384018743450447	6.841008241476645E-5
120-124	0.2998299895435189	6.590171272622501E-4	5.860463726864993E-4	6.818204880671724E-4	0.0036211736958216374	6.841008241476645E-5
125-129	0.3036677951669873	6.612974633427424E-4	6.248120860548668E-4	7.069041849525867E-4	0.0038195629348244603	6.841008241476645E-5
130-134	0.30937319604037883	6.681384715842189E-4	6.270924221353591E-4	7.2058620143554E-4	0.004038475198551712	6.841008241476645E-5
135-139	0.3148756470026065	6.863811602281567E-4	6.384941025378202E-4	7.297075457575089E-4	0.0042437054457960125	6.841008241476645E-5
140-144	0.3194728045408789	6.955025045501256E-4	6.544564551012658E-4	7.388288900794778E-4	0.004423851996154898	6.841008241476645E-5
145-149	0.3250345442411994	6.977828406306178E-4	6.612974633427424E-4	7.456698983209543E-4	0.004590316530030829	6.841008241476645E-5
150-154	0.33291082506321945	7.114648571135712E-4	6.612974633427424E-4	7.753142673673531E-4	0.004724856358779871	6.841008241476645E-5
155-159	0.3427892409639118	7.2058620143554E-4	6.612974633427424E-4	7.867159477698141E-4	0.004902722573058263	6.841008241476645E-5
160-164	0.3533152723114638	7.319878818380011E-4	6.681384715842189E-4	7.981176281722753E-4	0.005007618032760905	6.841008241476645E-5
165-169	0.3606921595318561	7.52510906562431E-4	6.726991437452035E-4	8.095193085747365E-4	0.005123915172866008	7.52510906562431E-5
>>END_MODULE
