##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479397
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8111130
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.253599683398	39.0	39.0	39.0	37.0	39.0
2	40.00254908995417	41.0	40.0	41.0	38.0	41.0
3	40.052663932152484	41.0	40.0	41.0	38.0	41.0
4	40.067866006339436	41.0	40.0	41.0	38.0	41.0
5	40.07376826656705	41.0	40.0	41.0	38.0	41.0
6	40.05830815188513	41.0	40.0	41.0	38.0	41.0
7	40.08190313803379	41.0	40.0	41.0	38.0	41.0
8	40.057394715656145	41.0	40.0	41.0	38.0	41.0
9	40.04205480617374	41.0	40.0	41.0	38.0	41.0
10-14	39.98260994460698	41.0	40.0	41.0	38.0	41.0
15-19	39.85225629474561	41.0	40.0	41.0	38.0	41.0
20-24	39.67632884690543	40.8	39.4	41.0	37.0	41.0
25-29	39.43882013973392	40.0	39.0	41.0	36.2	41.0
30-34	39.304209031294036	40.0	38.8	41.0	35.4	41.0
35-39	39.09415733689387	40.0	37.8	41.0	35.0	41.0
40-44	38.56324443080064	39.8	36.6	41.0	35.0	41.0
45-49	37.913543217776066	38.8	35.2	41.0	34.6	41.0
50-54	37.220225936460146	37.2	35.0	40.0	34.0	41.0
55-59	36.52423844766389	35.8	35.0	39.0	34.0	41.0
60-64	35.80153352245618	35.0	34.8	37.0	33.0	39.8
65-69	35.422827029032945	35.0	34.8	36.2	33.0	39.0
70-74	35.18987388933478	35.0	35.0	35.8	33.0	37.6
75-79	35.019280223593995	35.0	35.0	35.0	33.0	37.0
80-84	34.953632058665065	35.0	35.0	35.0	33.0	37.0
85-89	34.79115516580303	35.0	34.4	35.0	32.6	37.4
90-94	39.35907769891021	40.6	39.4	40.6	36.6	40.6
95-99	39.61831280008683	41.0	39.2	41.0	36.8	41.0
100-104	39.501117973406835	41.0	39.0	41.0	36.0	41.0
105-109	39.33683385573646	40.2	39.0	41.0	35.6	41.0
110-114	39.130226084996345	40.0	38.0	41.0	35.0	41.0
115-119	38.859834146850744	40.0	38.0	41.0	35.0	41.0
120-124	38.538720307287505	40.0	37.2	41.0	34.2	41.0
125-129	38.032647717314454	39.4	36.0	40.6	33.8	41.0
130-134	37.77861012836323	39.0	35.0	40.8	33.8	41.0
135-139	37.40529851682476	37.8	35.0	40.2	33.6	41.0
140-144	36.904561729019875	36.6	35.0	39.6	33.8	41.0
145-149	36.236570650029876	35.4	35.0	38.4	33.0	40.8
150-154	35.60846671785453	35.0	35.0	36.8	33.0	39.2
155-159	35.10728203148018	35.0	35.0	36.0	33.0	37.6
160-164	34.74887654926188	35.0	35.0	35.0	33.0	36.4
165-169	34.52477376476644	35.0	34.0	35.0	33.0	36.0
170-174	34.39375925584462	35.0	34.0	35.0	33.0	35.6
175-178	34.10734645023045	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	60.0
30	617.0
31	4272.0
32	21977.0
33	82886.0
34	241151.0
35	596213.0
36	1424833.0
37	2574645.0
38	2549289.0
39	607667.0
40	7517.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.73420596143817	26.92253730368025	27.373312966257473	22.969943768624102
2	23.239425332845116	27.329558273631417	27.318252820507134	22.112763573016338
3	21.477969160893736	26.50101773735595	28.501219928665922	23.51979317308439
4	22.284391940457123	25.740593480809704	28.861514980033608	23.113499598699562
5	23.042560037873887	26.283452490590093	27.412715614223913	23.261271857312114
6	23.283463586454662	26.313830502038556	27.018565847175424	23.38414006433136
7	23.12231464666452	26.70820218637847	26.836075861193198	23.33340730576381
8	23.277311545986812	26.710470674246373	26.7161049077009	23.296112872065912
9	23.519817830561216	26.697846046111945	26.381453632231267	23.40088249109557
10-14	23.36021738031569	26.835301616420892	26.490410090825815	23.3140709124376
15-19	23.32865580997962	26.77699408097269	26.5755277008259	23.31882240822179
20-24	23.26690855651432	26.646903698991387	26.746157440455278	23.34003030403902
25-29	23.29320575554824	26.650553005561495	26.589801914159928	23.46643932473034
30-34	23.371273792924043	26.698607962145843	26.546116262468978	23.384001982461136
35-39	23.358553000629996	26.640122892864497	26.56830799161153	23.433016114893977
40-44	23.314430911599246	26.66760365078602	26.61513993734535	23.402825500269383
45-49	23.4937178913419	26.587119180681363	26.48368353114794	23.4354793968288
50-54	23.440657466962065	26.634814138104062	26.50882922601413	23.415699168919744
55-59	23.399743315666253	26.62081608851048	26.525167270158413	23.454273325664857
60-64	23.42168847004055	26.63393879767677	26.497412814244132	23.446959918038544
65-69	23.397240581768507	26.608598308743662	26.52843685158541	23.465724257902412
70-74	23.321759113711654	26.66170311658178	26.54801735393219	23.468520415774375
75-79	23.351574934688507	26.62931798651976	26.53526943841364	23.4838376403781
80-84	23.331104297428347	26.653174095348	26.503167869335098	23.512553737888556
85-89	23.29184712857518	26.65325053352616	26.511716616550345	23.54318572134832
90-94	22.520834935457835	26.654572651181073	27.72501708096969	23.099575332391403
95-99	23.075437522991745	26.7986614488404	26.728195008427075	23.39770601974078
100-104	23.216298918727883	26.881419250775284	26.511628114278395	23.39065371621843
105-109	23.079688549631065	26.881880573123222	26.673978149137945	23.364452728107768
110-114	23.109676823012208	26.709125338744627	26.73900389152366	23.4421939467195
115-119	23.166748355682998	26.740468288880688	26.5811703176235	23.511613037812815
120-124	23.093267999095733	26.79065292530526	26.628166950969597	23.48791212462941
125-129	23.13924795597073	26.749042426382136	26.60742272597687	23.50428689167027
130-134	23.24511050263031	26.716222436308044	26.540572939274305	23.49809412178734
135-139	23.326942304070474	26.718609285680532	26.493687141526305	23.46076126872268
140-144	23.33832610276919	26.766317319434023	26.536065405152893	23.35929117264389
145-149	23.293367702220216	26.75872737534751	26.568391264293428	23.379513658138844
150-154	23.17868763968822	26.88027834969395	26.626375856132984	23.314658154484846
155-159	23.18189848743824	26.8847067228216	26.653343315959294	23.280051473780865
160-164	23.124063501412564	26.96134279075609	26.671035271759415	23.243558436071933
165-169	23.004186953149613	27.004667734103975	26.786671046622075	23.204474266124336
170-174	22.97350095606286	27.13702723487181	26.78557530958674	23.103896499478587
175-178	22.91018499749309	27.15945991375399	26.814244190776886	23.116110897976032
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	4.5
11	12.0
12	19.0
13	32.0
14	69.0
15	130.0
16	227.5
17	409.5
18	703.5
19	1263.0
20	2143.0
21	3475.5
22	5632.0
23	8597.5
24	12471.0
25	17735.5
26	24510.5
27	32634.5
28	42447.0
29	54086.5
30	67447.5
31	82646.5
32	99540.5
33	118125.5
34	139092.0
35	161957.0
36	185791.0
37	210658.0
38	236018.0
39	261673.5
40	285960.0
41	307851.0
42	325736.0
43	338950.0
44	347903.5
45	349948.0
46	346360.5
47	338273.0
48	325384.5
49	309071.5
50	289982.5
51	269975.0
52	249656.0
53	230067.0
54	212697.0
55	196342.5
56	181867.0
57	169664.0
58	158449.0
59	147837.5
60	137340.0
61	126672.5
62	115821.0
63	104411.5
64	92615.5
65	80376.0
66	68331.5
67	57004.5
68	46177.5
69	36478.5
70	28117.0
71	21117.0
72	15318.5
73	10800.5
74	7370.0
75	4839.5
76	3134.0
77	1966.5
78	1202.5
79	712.0
80	406.5
81	232.0
82	121.0
83	58.0
84	32.0
85	15.5
86	2.0
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1662.0
92-93	3464.0
94-95	5063.0
96-97	6506.0
98-99	7582.0
100-101	9575.0
102-103	9967.0
104-105	11646.0
106-107	12644.0
108-109	13473.0
110-111	14724.0
112-113	15948.0
114-115	16602.0
116-117	16999.0
118-119	19104.0
120-121	21491.0
122-123	19733.0
124-125	21492.0
126-127	22547.0
128-129	24763.0
130-131	25091.0
132-133	26723.0
134-135	127493.0
136-137	97663.0
138-139	95955.0
140-141	96350.0
142-143	98921.0
144-145	95914.0
146-147	100689.0
148-149	93932.0
150-151	97134.0
152-153	93622.0
154-155	96960.0
156-157	94047.0
158-159	99105.0
160-161	110012.0
162-163	95447.0
164-165	108685.0
166-167	112960.0
168-169	111805.0
170-171	113965.0
172-173	113118.0
174-175	117707.0
176-177	262436.0
178-179	5350411.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.58314370509959
#Duplication Level	Percentage of total
1	93.52215591520596
2	5.608873465482247
3	0.5950241325007677
4	0.1659669201732515
5	0.035856927489144465
6	0.029892934212653103
7	0.010371235370485194
8	0.005217408719138233
9	0.008398923594926912
>10	0.018242137251450644
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2328738412526985E-5	0.0	0.0	1.2328738412526985E-5	0.0	0.0
2	5.178070133261334E-4	2.465747682505397E-5	0.0	1.2328738412526985E-5	0.0	0.0
3	5.178070133261334E-4	4.931495365010794E-5	0.0	1.2328738412526985E-5	0.0	0.0
4	5.178070133261334E-4	8.63011688876889E-5	1.2328738412526985E-5	2.465747682505397E-5	0.0	0.0
5	5.178070133261334E-4	8.63011688876889E-5	2.465747682505397E-5	3.6986215237580954E-5	0.0	0.0
6	5.178070133261334E-4	8.63011688876889E-5	3.6986215237580954E-5	4.931495365010794E-5	0.0	0.0
7	5.178070133261334E-4	8.63011688876889E-5	3.6986215237580954E-5	4.931495365010794E-5	0.0	0.0
8	5.178070133261334E-4	8.63011688876889E-5	3.6986215237580954E-5	4.931495365010794E-5	0.0	0.0
9	5.424644901511873E-4	1.1095864571274285E-4	4.931495365010794E-5	6.164369206263492E-5	1.2328738412526985E-5	0.0
10-14	5.473959855161982E-4	1.2082163644276443E-4	4.9314953650107934E-5	6.904093511015111E-5	2.4657476825053967E-5	0.0
15-19	5.745192100237575E-4	1.7999958082289398E-4	5.9177944380129517E-5	7.397243047516191E-5	3.205471987257016E-5	0.0
20-24	6.041081822138223E-4	2.2191729142548568E-4	7.64381781576673E-5	1.2575313180777524E-4	4.9314953650107934E-5	0.0
25-29	6.336971544038869E-4	2.3424602983801267E-4	8.63011688876889E-5	1.3808187022030223E-4	5.9177944380129517E-5	0.0
30-34	6.854778557365004E-4	2.8849247885313147E-4	9.616415961771048E-5	1.4794486095032382E-4	6.904093511015111E-5	0.0
35-39	8.161624829092865E-4	3.378074325032394E-4	1.1589014107775365E-4	1.5780785168034538E-4	1.2821887949028064E-4	0.0
40-44	9.591758484945994E-4	3.969853768833689E-4	1.504106086328292E-4	1.652050947278616E-4	2.0465705764794793E-4	4.931495365010794E-6
45-49	0.0010602715034773208	4.635605643110146E-4	1.9972556228293716E-4	1.8986257155291557E-4	2.860267311706261E-4	1.2328738412526983E-5
50-54	0.0011515041677300204	5.030125272311011E-4	2.1698579606047491E-4	2.4410902056803429E-4	3.378074325032394E-4	1.2328738412526983E-5
55-59	0.0012032848690626337	5.547932285637143E-4	2.3424602983801267E-4	2.909582265356369E-4	3.8465663847084194E-4	1.2328738412526983E-5
60-64	0.0012452025796652255	6.041081822138223E-4	2.539720112980559E-4	3.304101894557232E-4	4.4383458285097136E-4	1.2328738412526983E-5
65-69	0.0013093120194103659	6.583546312289411E-4	2.589035066630667E-4	3.5753341396328254E-4	4.758893027235416E-4	2.4657476825053967E-5
70-74	0.001689037162516197	6.978065941490273E-4	2.638350020280775E-4	4.1424561066090665E-4	5.424644901511873E-4	2.4657476825053967E-5
75-79	0.009796415542593941	7.594502862116623E-4	2.7123224507559366E-4	4.610948166285093E-4	5.868479484362845E-4	2.958897219006476E-5
80-84	0.01344572211270193	7.767105199892E-4	2.8356098348812067E-4	4.90683788818574E-4	6.904093511015113E-4	3.6986215237580954E-5
85-89	0.013549283515367156	8.161624829092865E-4	3.057527126306693E-4	5.424644901511873E-4	9.271211286220292E-4	3.6986215237580954E-5
90-94	0.013608461459747284	8.457514550993513E-4	3.7232790005831487E-4	5.893136961187898E-4	9.369841193520507E-4	3.6986215237580954E-5
95-99	0.013633118936572338	8.999979041144699E-4	3.9451962920086347E-4	6.386286497688978E-4	9.419156147170615E-4	3.6986215237580954E-5
100-104	0.013672570899492425	9.147923902095023E-4	3.9451962920086347E-4	6.978065941490273E-4	9.493128577645778E-4	3.6986215237580954E-5
105-109	0.01372928309619005	9.24655380939524E-4	3.9451962920086347E-4	7.421900524341244E-4	9.493128577645778E-4	3.6986215237580954E-5
110-114	0.01375640632069761	9.394498670345563E-4	4.0684836761339054E-4	7.64381781576673E-4	9.517786054470832E-4	3.6986215237580954E-5
115-119	0.013803255526665212	9.56710100812094E-4	4.167113583434121E-4	8.136967352267811E-4	9.838333253196533E-4	3.6986215237580954E-5
120-124	0.013931474406155492	0.0010035593067796967	4.2657434907343377E-4	8.605459411943836E-4	0.0010010935590971913	3.6986215237580954E-5
125-129	0.014121336977708409	0.0010578057557948152	4.53697573580993E-4	9.172581378920077E-4	0.0010331482789697614	3.6986215237580954E-5
130-134	0.014353117259863915	0.0010947919710323963	4.808207980885524E-4	9.369841193520507E-4	0.0010775317372548586	3.6986215237580954E-5
135-139	0.014592294785066939	0.0011416411769999987	5.153412656436279E-4	9.468471100820724E-4	0.0011293124385874718	3.6986215237580954E-5
140-144	0.014737773898334758	0.0011613671584600421	5.52327480881209E-4	9.715045869071263E-4	0.0011662986538250528	3.6986215237580954E-5
145-149	0.014870924273190051	0.0012032848690626337	5.547932285637143E-4	0.0010134222975097182	0.0012032848690626337	3.6986215237580954E-5
150-154	0.01496215693744275	0.0012698600564902793	5.547932285637143E-4	0.0010528742604298045	0.0012575313180777526	3.6986215237580954E-5
155-159	0.015107636050710565	0.001304380524045355	5.671219669762413E-4	0.0011071207094449232	0.0012969832809978387	3.6986215237580954E-5
160-164	0.015302430117628493	0.0013462982346479466	5.671219669762413E-4	0.0011317781862699773	0.0013536954776954629	3.6986215237580954E-5
165-167	0.015439690071954627	0.0013684899637904952	5.671219669762413E-4	0.0011465726723650096	0.0013684899637904952	4.109579470842328E-5
>>END_MODULE
