##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479398
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255084
Total Bases	43.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.404239387809504	39.0	39.0	39.0	37.0	39.0
2	40.16674115193427	41.0	40.0	41.0	38.0	41.0
3	40.17467971334933	41.0	40.0	41.0	38.0	41.0
4	40.179576139624594	41.0	40.0	41.0	38.0	41.0
5	40.172919508867665	41.0	40.0	41.0	38.0	41.0
6	40.164910382462246	41.0	40.0	41.0	38.0	41.0
7	40.17624390396889	41.0	40.0	41.0	38.0	41.0
8	40.187742077119694	41.0	40.0	41.0	38.0	41.0
9	40.17152389016951	41.0	40.0	41.0	38.0	41.0
10-14	40.10485173511471	41.0	40.0	41.0	38.0	41.0
15-19	39.97360006899688	41.0	40.0	41.0	38.0	41.0
20-24	39.79859575669191	41.0	40.0	41.0	37.6	41.0
25-29	39.53523153157391	40.2	39.0	41.0	36.6	41.0
30-34	39.38976886045381	40.0	39.0	41.0	36.0	41.0
35-39	39.1969853068009	40.0	38.2	41.0	35.0	41.0
40-44	38.655876495585765	40.0	36.8	41.0	35.0	41.0
45-49	37.98922237380627	38.8	35.2	41.0	34.8	41.0
50-54	37.280456633893145	37.2	35.0	40.0	34.0	41.0
55-59	36.56107007887598	35.8	35.0	39.0	34.0	41.0
60-64	35.81289928023709	35.0	34.8	37.0	33.4	39.6
65-69	35.4268256731116	35.0	35.0	36.0	33.0	39.0
70-74	35.195665741481236	35.0	35.0	35.8	33.0	37.2
75-79	35.02105188878958	35.0	35.0	35.0	33.0	37.0
80-84	34.94061877655988	35.0	35.0	35.0	33.0	36.2
85-89	34.755827100092525	35.0	34.6	35.0	32.6	36.6
90-94	39.543619091171685	40.6	39.8	40.6	37.8	40.6
95-99	39.80284336034848	41.0	40.0	41.0	37.4	41.0
100-104	39.69041882480093	41.0	39.8	41.0	37.0	41.0
105-109	39.53116937084905	41.0	39.0	41.0	36.0	41.0
110-114	39.32477001847022	40.2	38.8	41.0	35.2	41.0
115-119	39.052130143313185	40.0	38.0	41.0	35.0	41.0
120-124	38.717642311833984	40.0	37.6	41.0	35.0	41.0
125-129	38.160156155846074	39.4	36.4	40.6	34.0	40.8
130-134	37.90765075711656	39.0	35.4	40.8	34.0	41.0
135-139	37.548366396052224	38.0	35.0	41.0	34.0	41.0
140-144	37.04542433875255	36.8	35.0	39.8	34.0	41.0
145-149	36.34379324961814	35.6	35.0	38.6	33.8	40.8
150-154	35.68889445208549	35.0	35.0	36.8	33.0	39.2
155-159	35.18069199987149	35.0	35.0	36.0	33.0	37.6
160-164	34.791111786330276	35.0	35.0	35.0	33.0	36.4
165-169	34.5745207753762	35.0	34.8	35.0	33.0	36.0
170-174	34.43062312251991	35.0	34.0	35.0	33.0	35.6
175-178	34.13861709790778	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	6.0
31	75.0
32	484.0
33	2023.0
34	6095.0
35	16031.0
36	42750.0
37	82868.0
38	84720.0
39	19841.0
40	190.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.826206269307363	26.75706825986734	27.35569459472174	23.06103087610356
2	23.347603142494236	27.220837057596714	27.263568079534583	22.167991720374463
3	21.631305765943768	26.305452321588184	28.441219363033355	23.622022549434696
4	22.405560521240062	25.626068275548448	28.826582615922597	23.141788587288893
5	23.25390851641028	26.188628059776388	27.166737231657024	23.390726192156308
6	23.477756346928853	26.247432218406487	26.90055040692478	23.37426102773988
7	23.171974722052344	26.60143325335968	26.796663060011603	23.429928964576373
8	23.37269291684308	26.63318749901993	26.57399131266563	23.42012827147136
9	23.682002791237398	26.65749321792037	26.132960122939895	23.527543867902338
10-14	23.45627322764266	26.76616330306879	26.399068542127303	23.37849492716125
15-19	23.453293816938732	26.636010098634177	26.468535854855656	23.442160229571435
20-24	23.389393297894028	26.51832337582914	26.638519076069063	23.453764250207776
25-29	23.34846560348748	26.554076304276236	26.469398315848895	23.628059776387385
30-34	23.489752395289397	26.557369337159525	26.40087186965862	23.55200639789246
35-39	23.411817283718307	26.599316303648994	26.44775838547302	23.54110802715968
40-44	23.47038622571388	26.562230480939615	26.42886264916655	23.538520644179954
45-49	23.544714682222327	26.60347179752552	26.293848300951844	23.557965219300307
50-54	23.59795204716878	26.528280880023836	26.320976619466528	23.55279045334086
55-59	23.497671355318246	26.562936130843173	26.408085179783914	23.531307334054663
60-64	23.586818459801478	26.50193661695755	26.356729547913627	23.55451537532734
65-69	23.496024838876608	26.57869564535604	26.420865283592853	23.5044142321745
70-74	23.491555722820717	26.565209891643537	26.35939533643819	23.583839049097552
75-79	23.40538802904141	26.59273023788242	26.39961738094118	23.602264352134984
80-84	23.491163695096517	26.536043028963007	26.35837606435527	23.614417211585202
85-89	23.502218876918977	26.583164761411926	26.277775164259616	23.63684119740948
90-94	22.648858817034302	26.575243418906368	27.59581482596803	23.180082938091296
95-99	23.20006595322032	26.622802540769296	26.629947472970954	23.54718403303942
100-104	23.29650483206927	26.799696058673888	26.40852105485889	23.495278054397946
105-109	23.18709356847373	26.737072076157727	26.564896935471786	23.510937419896756
110-114	23.22270754802113	26.595308470368828	26.6596638322866	23.522320149323438
115-119	23.28239268159085	26.626540601604777	26.494889372422726	23.59617734438165
120-124	23.143999098852237	26.634348908756394	26.556776703093384	23.66487528929799
125-129	23.199382395210833	26.685941377351867	26.525033157697287	23.589643069740017
130-134	23.37361631671027	26.609082589278493	26.410605085551488	23.60669600845975
135-139	23.389171215258173	26.562205910032	26.462896371363193	23.585726503346642
140-144	23.449609116654514	26.616018586036734	26.40421238277739	23.530159914531364
145-149	23.435460399620073	26.6777333368189	26.383203060326423	23.503603203234604
150-154	23.274054020552995	26.798926092029497	26.526496945613875	23.400522941803636
155-159	23.2611327488671	26.753028463110507	26.56060258270888	23.42523620531351
160-164	23.199040955523643	26.87142355533402	26.630035964167863	23.29949952497448
165-169	23.06252990305949	26.992064894354108	26.63570976214536	23.309695440441047
170-174	23.01821053417014	27.074230829816692	26.65669018463401	23.25086845137916
175-178	23.02590354552736	27.01727811166749	26.731625319435288	23.225193023369858
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	1.0
13	2.0
14	3.0
15	4.0
16	5.5
17	12.5
18	25.5
19	33.5
20	56.5
21	105.0
22	148.0
23	237.0
24	367.0
25	509.0
26	730.5
27	982.5
28	1287.5
29	1625.5
30	2015.5
31	2466.0
32	3058.0
33	3675.0
34	4203.5
35	4878.0
36	5627.5
37	6475.0
38	7348.5
39	8162.0
40	8880.5
41	9558.0
42	10076.5
43	10464.5
44	10823.5
45	11006.0
46	10943.5
47	10539.5
48	10053.5
49	9630.0
50	9195.0
51	8606.0
52	7986.0
53	7399.0
54	6832.5
55	6376.5
56	5933.0
57	5543.5
58	5146.5
59	4754.0
60	4404.5
61	4043.5
62	3751.5
63	3454.5
64	3021.0
65	2647.0
66	2303.5
67	1882.5
68	1503.0
69	1185.0
70	907.5
71	677.0
72	506.5
73	355.0
74	236.0
75	159.0
76	105.5
77	69.0
78	48.5
79	30.0
80	14.5
81	9.0
82	8.5
83	4.0
84	1.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	35.0
92-93	91.0
94-95	149.0
96-97	171.0
98-99	164.0
100-101	196.0
102-103	265.0
104-105	268.0
106-107	309.0
108-109	373.0
110-111	394.0
112-113	427.0
114-115	429.0
116-117	458.0
118-119	499.0
120-121	495.0
122-123	535.0
124-125	555.0
126-127	598.0
128-129	665.0
130-131	590.0
132-133	737.0
134-135	3174.0
136-137	2571.0
138-139	2525.0
140-141	2535.0
142-143	2540.0
144-145	2488.0
146-147	2631.0
148-149	2808.0
150-151	2775.0
152-153	2662.0
154-155	2598.0
156-157	2564.0
158-159	2866.0
160-161	2877.0
162-163	2955.0
164-165	3001.0
166-167	3183.0
168-169	3495.0
170-171	3292.0
172-173	3735.0
174-175	3690.0
176-177	8602.0
178-179	177114.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.84792430843132
#Duplication Level	Percentage of total
1	99.69635417602281
2	0.30212914649672173
3	0.0015166774804602438
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	3.920277242006555E-4	0.0	0.0	0.0	0.0	0.0
3	3.920277242006555E-4	0.0	0.0	0.0	0.0	0.0
4	3.920277242006555E-4	0.0	0.0	0.0	0.0	0.0
5	3.920277242006555E-4	0.0	0.0	0.0	0.0	0.0
6	3.920277242006555E-4	0.0	0.0	0.0	0.0	0.0
7	3.920277242006555E-4	0.0	0.0	0.0	0.0	0.0
8	3.920277242006555E-4	0.0	0.0	0.0	0.0	0.0
9	3.920277242006555E-4	0.0	0.0	0.0	0.0	0.0
10-14	3.9202772420065553E-4	0.0	0.0	0.0	0.0	0.0
15-19	3.9202772420065553E-4	0.0	3.1362217936052437E-4	0.0	0.0	0.0
20-24	3.9202772420065553E-4	0.0	7.840554484013111E-4	0.0	0.0	0.0
25-29	3.9202772420065553E-4	0.0	7.840554484013111E-4	0.0	0.0	0.0
30-34	3.9202772420065553E-4	0.0	7.840554484013111E-4	0.0	0.0	0.0
35-39	3.9202772420065553E-4	0.0	7.840554484013111E-4	0.0	0.0	0.0
40-44	3.9202772420065553E-4	0.0	7.840554484013111E-4	0.0	0.0	0.0
45-49	7.840554484013111E-4	0.0	7.840554484013111E-4	0.0	0.0	0.0
50-54	7.840554484013111E-4	0.0	7.840554484013111E-4	0.0	0.0	0.0
55-59	8.62460993241442E-4	0.0	7.840554484013111E-4	7.840554484013109E-5	0.0	0.0
60-64	0.0011760831726019664	0.0	7.840554484013111E-4	3.9202772420065553E-4	0.0	0.0
65-69	0.0011760831726019664	0.0	7.840554484013111E-4	3.9202772420065553E-4	0.0	0.0
70-74	0.001724921986482884	0.0	7.840554484013111E-4	3.9202772420065553E-4	0.0	0.0
75-79	0.010819965187938091	0.0	7.840554484013111E-4	3.9202772420065553E-4	1.5681108968026219E-4	0.0
80-84	0.014112998071223595	0.0	7.840554484013111E-4	3.9202772420065553E-4	3.9202772420065553E-4	0.0
85-89	0.014112998071223595	0.0	7.840554484013111E-4	7.056499035611799E-4	3.9202772420065553E-4	0.0
90-94	0.014112998071223595	2.352166345203933E-4	7.840554484013111E-4	7.840554484013111E-4	3.9202772420065553E-4	0.0
95-99	0.014112998071223595	3.9202772420065553E-4	7.840554484013111E-4	7.840554484013111E-4	3.9202772420065553E-4	0.0
100-104	0.014112998071223595	3.9202772420065553E-4	7.840554484013111E-4	8.62460993241442E-4	3.9202772420065553E-4	0.0
105-109	0.014112998071223595	3.9202772420065553E-4	0.0010976776277618352	0.0011760831726019664	3.9202772420065553E-4	0.0
110-114	0.014112998071223595	3.9202772420065553E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
115-119	0.014112998071223595	3.9202772420065553E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
120-124	0.014112998071223595	3.9202772420065553E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
125-129	0.014112998071223595	3.9202772420065553E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
130-134	0.014112998071223595	3.9202772420065553E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
135-139	0.014505025795424254	3.9202772420065553E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
140-144	0.01489705351962491	3.9202772420065553E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
145-149	0.01489705351962491	4.704332690407866E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
150-154	0.01489705351962491	7.840554484013111E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
155-159	0.01489705351962491	7.840554484013111E-4	0.0011760831726019664	0.0011760831726019664	7.840554484013111E-4	0.0
160-164	0.01505386460930517	7.840554484013111E-4	0.0011760831726019664	0.0011760831726019664	0.0011760831726019664	0.0
165-167	0.015289081243825562	7.84055448401311E-4	0.0011760831726019664	0.0011760831726019664	0.0011760831726019664	0.0
>>END_MODULE
