##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479409
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4046040
Total Bases	651.9 Mbp
Sequences flagged as poor quality	0
Sequence length	90-180
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.145915018141196	41.0	40.0	41.0	38.0	41.0
2	40.217725974038814	41.0	40.0	41.0	38.0	41.0
3	40.24094472620142	41.0	40.0	41.0	39.0	41.0
4	40.22770882146494	41.0	40.0	41.0	38.0	41.0
5	40.19653068185188	41.0	40.0	41.0	38.0	41.0
6	40.162131862265326	41.0	40.0	41.0	38.0	41.0
7	40.127275558323696	41.0	40.0	41.0	38.0	41.0
8	40.09450648041047	41.0	40.0	41.0	38.0	41.0
9	40.042100918428886	41.0	40.0	41.0	38.0	41.0
10-14	39.91368928631452	41.0	40.0	41.0	38.0	41.0
15-19	39.89796339136538	41.0	40.0	41.0	38.0	41.0
20-24	39.66007918853991	40.6	39.4	41.0	37.2	41.0
25-29	39.31990479580034	40.0	38.8	41.0	35.8	41.0
30-34	39.02567008729523	40.0	37.8	41.0	35.0	41.0
35-39	38.345658174412506	39.4	36.4	41.0	34.8	41.0
40-44	37.473112376545956	37.8	35.0	40.2	34.0	41.0
45-49	36.54187279413945	36.4	35.0	39.2	33.0	41.0
50-54	35.76826679914187	35.0	34.8	37.8	32.8	39.8
55-59	35.18860802167057	35.0	34.0	36.6	32.0	39.0
60-64	34.77739310535734	34.8	33.6	35.8	31.8	39.0
65-69	34.98446619410584	35.0	34.0	35.2	32.4	39.2
70-74	34.95363802631709	35.0	34.0	35.0	32.0	40.0
75-79	35.04075718480292	35.0	34.0	35.6	31.2	40.2
80-84	35.21687976391731	35.0	34.0	37.4	31.0	41.0
85-89	35.936107848661905	35.8	34.4	39.0	31.4	41.0
90-94	39.62586264722644	41.0	39.4	41.0	36.8	41.0
95-99	39.389649319765354	40.0	39.0	41.0	35.8	41.0
100-104	39.247743439363504	40.0	38.8	41.0	35.0	41.0
105-109	39.03777236281072	40.0	38.0	41.0	35.0	41.0
110-114	38.725960521451356	40.0	37.6	41.0	34.6	41.0
115-119	38.332798748805786	40.0	36.6	41.0	34.0	41.0
120-124	37.85687357610698	39.0	35.2	40.8	33.4	41.0
125-129	37.09127963483018	38.0	35.0	39.8	33.0	40.6
130-134	36.84701078441514	37.2	35.0	40.0	33.0	41.0
135-139	36.24412374602084	35.8	35.0	39.0	33.0	41.0
140-144	35.581208578313635	35.0	34.0	37.6	32.0	39.8
145-149	34.88413102028416	35.0	34.0	36.2	31.4	38.6
150-154	34.301697783916985	35.0	33.8	35.2	31.0	36.8
155-159	33.8829895485227	35.0	33.0	35.0	31.0	36.0
160-164	33.5783251052395	34.6	33.0	35.0	30.0	35.0
165-169	33.36183517441039	34.0	33.0	35.0	30.0	35.0
170-174	33.18679123751517	34.0	32.6	35.0	29.2	35.0
175-179	33.015684044576105	34.0	32.0	35.0	29.0	35.0
180	30.256319399047847	31.0	27.0	34.0	25.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	13.0
31	288.0
32	2542.0
33	16371.0
34	84702.0
35	382746.0
36	1192934.0
37	1721721.0
38	622911.0
39	21630.0
40	182.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.09836778677423	24.549287698589236	25.245301578827693	25.10704293580884
2	24.771480262182283	24.750892230427777	25.341444968413562	25.13618253897638
3	24.748099376180164	24.859121511403742	25.157437889887397	25.235341222528696
4	24.92951132465324	25.052594635742604	24.86132119306779	25.156572846536367
5	24.974098130517742	25.088135559707762	24.88279898369764	25.054967326076856
6	24.724298326264694	25.084131644768714	24.911543138476137	25.280026890490454
7	25.032377336852825	25.080770333461857	24.717674565748236	25.169177763937086
8	25.13781376358118	25.086825636919063	24.755217447182925	25.02014315231683
9	24.83329378849443	24.968116973633478	24.819799112218366	25.378790125653726
10-14	24.961740368360175	25.037448962442287	24.849388537928444	25.15142213126909
15-19	24.890525056598552	24.90746507696414	24.957588160275233	25.244421706162072
20-24	24.92683216181748	24.895221006218428	24.905329655663316	25.272617176300777
25-29	24.98120631531077	24.934004606973733	24.794668367094737	25.290120710620755
30-34	24.935887929926544	24.905937657561466	24.84090122687863	25.31727318563336
35-39	24.884212711688466	24.945650562031023	24.88933376832656	25.280802957953952
40-44	25.025536079722393	24.91379719429368	24.750699449338118	25.309967276645807
45-49	25.17857954938656	24.846343585332818	24.64078456960386	25.334292295676757
50-54	25.1718223250388	24.82985338750976	24.67809018200512	25.320234105446314
55-59	25.12084408458641	24.846027226621587	24.674456999930797	25.358671688861207
60-64	25.022085792528003	24.850940672855433	24.74413994918488	25.38283358543168
65-69	25.037018912319205	24.86360985061937	24.757847178970056	25.34152405809137
70-74	24.98824035353086	24.84946268450139	24.770051704876867	25.392245257090885
75-79	24.931888958092358	24.800357880792085	24.798098881869677	25.46965427924588
80-84	24.875942897252624	24.79648248657947	24.89408409209993	25.433490524067974
85-89	24.842038140997122	24.64722049213552	25.023054641081156	25.4876867257862
90-94	24.66528863562515	24.665085891889227	25.64933255525892	25.020292917226705
95-99	24.95638302570045	24.887454255020888	24.991377708600105	25.164785010678557
100-104	25.015683916593478	24.89128298843819	24.934875837796962	25.158157257171375
105-109	24.998329012834937	24.83273957505831	24.996202985654183	25.172728426452572
110-114	24.975514960402226	24.819082059839584	24.986541201742853	25.218861778015334
115-119	24.969498476820892	24.844913418537296	24.960551568441748	25.22503653620006
120-124	24.947407517773986	24.889597087934444	24.956709926253527	25.206285468038043
125-129	24.92241757284328	24.916178319664333	24.980177206492208	25.18122690100018
130-134	25.035440816484773	24.90356089972332	24.92219183198525	25.138806451806662
135-139	25.027559375025287	24.958941574124946	24.953004043105338	25.060495007744432
140-144	24.96323097304034	25.028220329746816	25.022852193235824	24.985696503977024
145-149	24.885860840283488	25.12003178680758	25.0760103961861	24.91809697672284
150-154	24.811966871020072	25.210375619962544	25.164810521261956	24.812846987755428
155-159	24.646730265149813	25.292037752668524	25.289281840271105	24.77195014191056
160-164	24.57085853314964	25.39304612713911	25.433106036949795	24.602989302761454
165-169	24.404530322126732	25.546982930335172	25.582559292642905	24.46592745489519
170-174	24.210295897905056	25.65587082882833	25.835620489220336	24.29821278404628
175-179	23.847256752247773	25.829773533949997	26.27551405103828	24.04745566276395
180	25.163370007368684	24.3379154703139	26.34732613712224	24.151388385195176
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.5
18	3.0
19	5.5
20	9.0
21	23.5
22	50.0
23	112.5
24	250.0
25	451.5
26	769.5
27	1260.5
28	2034.0
29	3119.5
30	4716.5
31	6930.0
32	9897.0
33	13893.0
34	19015.0
35	25455.0
36	33750.5
37	43992.0
38	55929.0
39	69942.5
40	85781.0
41	102640.0
42	120364.0
43	138665.5
44	156394.0
45	172800.5
46	187360.0
47	198473.0
48	205770.0
49	209292.5
50	207819.5
51	202074.5
52	193023.0
53	181230.5
54	167246.0
55	152074.0
56	137034.5
57	123010.0
58	111233.5
59	100890.0
60	91091.0
61	82319.0
62	73911.5
63	65710.5
64	57822.0
65	49710.0
66	41930.5
67	34807.0
68	27980.0
69	21850.5
70	16624.5
71	12221.0
72	8807.0
73	6272.0
74	4246.0
75	2681.5
76	1728.0
77	1046.0
78	591.5
79	356.5
80	190.0
81	89.5
82	48.0
83	21.5
84	4.0
85	1.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1178.0
92-93	2559.0
94-95	3977.0
96-97	5324.0
98-99	6840.0
100-101	8541.0
102-103	10359.0
104-105	11738.0
106-107	13369.0
108-109	14782.0
110-111	16238.0
112-113	18426.0
114-115	20379.0
116-117	22637.0
118-119	24483.0
120-121	26919.0
122-123	29217.0
124-125	31544.0
126-127	34380.0
128-129	36797.0
130-131	40055.0
132-133	55759.0
134-135	65388.0
136-137	69251.0
138-139	74213.0
140-141	77441.0
142-143	80779.0
144-145	83311.0
146-147	87412.0
148-149	92787.0
150-151	93763.0
152-153	94155.0
154-155	98855.0
156-157	102098.0
158-159	108867.0
160-161	115328.0
162-163	119058.0
164-165	123549.0
166-167	126827.0
168-169	130456.0
170-171	134483.0
172-173	138641.0
174-175	145198.0
176-177	153073.0
178-179	429780.0
180-181	865826.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.19744725354279
#Duplication Level	Percentage of total
1	91.15560728853328
2	7.175722086640388
3	0.9959371654861583
4	0.24145808050291453
5	0.11330616672909999
6	0.0775307298104988
7	0.05052977326708961
8	0.044617896991105035
9	0.03965343077804589
>10	0.10563738126142144
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	2.471552431513282E-5	0.0	0.0	0.0	0.0
4	0.0	2.471552431513282E-5	0.0	2.471552431513282E-5	0.0	0.0
5	0.0	2.471552431513282E-5	2.471552431513282E-5	4.943104863026564E-5	0.0	0.0
6	0.0	2.471552431513282E-5	2.471552431513282E-5	4.943104863026564E-5	0.0	0.0
7	0.0	4.943104863026564E-5	2.471552431513282E-5	4.943104863026564E-5	0.0	0.0
8	0.0	4.943104863026564E-5	2.471552431513282E-5	4.943104863026564E-5	0.0	0.0
9	0.0	7.414657294539846E-5	2.471552431513282E-5	4.943104863026564E-5	0.0	0.0
10-14	4.943104863026564E-6	7.414657294539846E-5	2.471552431513282E-5	5.437415349329221E-5	0.0	0.0
15-19	3.460173404118595E-5	9.886209726053128E-5	2.471552431513282E-5	8.40327826714516E-5	1.4829314589079692E-5	0.0
20-24	7.414657294539846E-5	1.0380520212355784E-4	2.471552431513282E-5	9.886209726053128E-5	2.471552431513282E-5	0.0
25-29	9.391899239750473E-5	1.3346383130171722E-4	4.448794376723908E-5	9.886209726053128E-5	2.471552431513282E-5	0.0
30-34	9.886209726053128E-5	1.7300867020592976E-4	7.414657294539846E-5	9.886209726053128E-5	3.9544838904212515E-5	0.0
35-39	9.886209726053128E-5	2.5704145287738133E-4	7.414657294539846E-5	1.235776215756641E-4	6.92034680823719E-5	0.0
40-44	1.235776215756641E-4	2.9658629178159384E-4	7.414657294539846E-5	1.235776215756641E-4	7.414657294539846E-5	0.0
45-49	1.235776215756641E-4	3.4107423554883296E-4	8.897588753447816E-5	1.235776215756641E-4	7.414657294539846E-5	2.471552431513282E-5
50-54	1.3346383130171722E-4	3.6578975986396574E-4	1.235776215756641E-4	1.235776215756641E-4	7.414657294539846E-5	2.471552431513282E-5
55-59	1.4829314589079692E-4	4.4982254253541737E-4	1.235776215756641E-4	1.680655653429032E-4	7.414657294539846E-5	2.471552431513282E-5
60-64	1.4829314589079692E-4	4.99253591165683E-4	1.384069361647438E-4	1.7300867020592976E-4	1.0874830698658442E-4	2.9658629178159384E-5
65-69	1.680655653429032E-4	5.239691154808158E-4	1.7300867020592976E-4	1.92781089658036E-4	1.235776215756641E-4	4.943104863026564E-5
70-74	4.1522080849423147E-4	5.585708495220018E-4	1.92781089658036E-4	1.9772419452106257E-4	1.828948799319829E-4	4.943104863026564E-5
75-79	0.0018783798479500944	6.030587932892408E-4	2.1749661397316885E-4	1.9772419452106257E-4	2.6692766260343444E-4	4.943104863026564E-5
80-84	0.0021502506154165556	6.426036321934533E-4	2.224397188361954E-4	2.174966139731688E-4	3.361311306858064E-4	4.943104863026564E-5
85-89	0.0021848523494577415	6.426036321934533E-4	2.669276626034345E-4	2.5704145287738133E-4	3.460173404118595E-4	4.943104863026564E-5
90-94	0.0022095678737728743	6.475467370564799E-4	2.867000820555407E-4	2.7187076746646105E-4	3.460173404118595E-4	4.943104863026564E-5
95-99	0.002224397188361954	6.821484710976659E-4	3.0647250150764695E-4	2.7187076746646105E-4	3.460173404118595E-4	4.943104863026564E-5
100-104	0.002224397188361954	7.167502051388518E-4	3.213018160967267E-4	3.213018160967267E-4	3.460173404118595E-4	4.943104863026564E-5
105-109	0.002224397188361954	7.36522624590958E-4	3.460173404118595E-4	3.213018160967267E-4	3.460173404118595E-4	4.943104863026564E-5
110-114	0.0022491127126770868	7.464088343170111E-4	3.707328647269923E-4	3.213018160967267E-4	3.460173404118595E-4	4.943104863026564E-5
115-119	0.0022491127126770868	7.908967780842503E-4	3.707328647269923E-4	3.213018160967267E-4	3.460173404118595E-4	4.943104863026564E-5
120-124	0.0022540558175401133	8.15612302399383E-4	3.85562179316072E-4	3.213018160967267E-4	3.460173404118595E-4	4.943104863026564E-5
125-129	0.0023183161807594586	8.15612302399383E-4	3.9544838904212513E-4	3.4107423554883296E-4	3.707328647269923E-4	4.943104863026564E-5
130-134	0.002347974809937618	8.205554072624096E-4	4.1522080849423147E-4	3.460173404118595E-4	3.707328647269923E-4	4.943104863026564E-5
135-139	0.002367747229389724	8.452709315775426E-4	4.20163913357258E-4	3.460173404118595E-4	3.85562179316072E-4	4.943104863026564E-5
140-144	0.0023776334391157773	8.848157704817551E-4	4.349932279463377E-4	3.460173404118595E-4	4.102777036312048E-4	4.943104863026564E-5
145-149	0.0024171782780199897	9.342468191120206E-4	4.547656473984439E-4	3.608466550009392E-4	4.20163913357258E-4	4.943104863026564E-5
150-154	0.002461666221787229	9.441330288380737E-4	4.943104863026564E-4	3.905052841790986E-4	4.20163913357258E-4	4.943104863026564E-5
155-159	0.0025012110606914416	9.639054482901801E-4	5.190260106177892E-4	3.9544838904212513E-4	4.20163913357258E-4	4.943104863026564E-5
160-164	0.002565471423910787	9.787347628792597E-4	5.190260106177892E-4	4.20163913357258E-4	4.20163913357258E-4	4.943104863026564E-5
165-169	0.0026149024725410524	0.0010133364969204456	5.190260106177892E-4	4.20163913357258E-4	4.20163913357258E-4	4.943104863026564E-5
>>END_MODULE
