##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479411
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3236688
Total Bases	516.2 Mbp
Sequences flagged as poor quality	0
Sequence length	90-180
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.1559041217442	41.0	40.0	41.0	38.0	41.0
2	40.216470972796884	41.0	40.0	41.0	38.0	41.0
3	40.24040871409292	41.0	40.0	41.0	39.0	41.0
4	40.220091649241446	41.0	40.0	41.0	38.0	41.0
5	40.19312550360121	41.0	40.0	41.0	38.0	41.0
6	40.15545984042948	41.0	40.0	41.0	38.0	41.0
7	40.12319877603279	41.0	40.0	41.0	38.0	41.0
8	40.08281613797808	41.0	40.0	41.0	38.0	41.0
9	40.03271121590959	41.0	40.0	41.0	38.0	41.0
10-14	39.90141218430692	40.8	40.0	41.0	38.0	41.0
15-19	39.90017073007964	41.0	40.0	41.0	38.0	41.0
20-24	39.66104375831096	40.4	39.4	41.0	37.2	41.0
25-29	39.32228419915667	40.0	38.8	41.0	35.8	41.0
30-34	39.01176406252317	40.0	37.8	41.0	35.0	41.0
35-39	38.324474833533536	39.4	36.4	41.0	34.8	41.0
40-44	37.43989590593842	37.8	35.0	40.2	33.8	41.0
45-49	36.506532665490155	36.4	35.0	39.2	33.0	40.8
50-54	35.73680997365208	35.0	34.6	37.8	32.8	39.8
55-59	35.1565943334668	35.0	34.0	36.6	32.0	39.0
60-64	34.750328360348604	34.8	33.6	35.8	31.4	39.0
65-69	34.96616627861567	35.0	34.0	35.4	32.0	39.4
70-74	34.94870268620268	35.0	34.0	35.0	31.8	40.0
75-79	35.06260257398922	35.0	34.0	36.0	31.0	40.4
80-84	35.260050026446784	35.0	34.0	38.0	31.0	41.0
85-89	36.00923159723767	35.8	34.4	39.4	31.4	41.0
90-94	39.58392999609073	40.8	39.2	41.0	36.6	41.0
95-99	39.32322671825957	40.0	38.8	41.0	35.6	41.0
100-104	39.17567362840465	40.0	38.4	41.0	35.0	41.0
105-109	38.949347855702456	40.0	38.0	41.0	35.0	41.0
110-114	38.61901755949826	40.0	37.4	41.0	34.2	41.0
115-119	38.21851575688686	39.8	36.4	41.0	34.0	41.0
120-124	37.736487069081704	39.0	35.2	40.6	33.0	41.0
125-129	36.9934026876329	37.8	35.0	39.8	32.8	40.6
130-134	36.73599712044757	37.0	35.0	40.0	32.6	41.0
135-139	36.138560244954604	35.8	34.6	39.0	32.0	40.8
140-144	35.48953370439392	35.0	34.0	37.4	31.8	39.8
145-149	34.78001896222833	35.0	34.0	36.2	31.0	38.4
150-154	34.19764243837439	35.0	33.2	35.0	31.0	36.8
155-159	33.759598689784255	35.0	33.0	35.0	30.2	35.8
160-164	33.443254460661834	34.0	33.0	35.0	30.0	35.0
165-169	33.22521593622533	34.0	32.6	35.0	29.4	35.0
170-174	33.036532478349535	34.0	32.0	35.0	29.0	35.0
175-179	32.855763935384395	34.0	31.8	35.0	29.0	35.0
180	30.08667729725941	31.0	27.0	34.0	25.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	10.0
31	164.0
32	1802.0
33	12331.0
34	66734.0
35	310471.0
36	977663.0
37	1378648.0
38	472504.0
39	16256.0
40	105.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.05431478103543	24.623102381199548	25.224148883055765	25.09843395470926
2	24.749836870282216	24.773688412352378	25.371181899521982	25.105292817843427
3	24.721011107650785	24.855747603723312	25.174993697260906	25.248247591365004
4	24.963481188177543	25.033676400073162	24.87925929221476	25.123583119534537
5	24.979392514817615	25.101307262238436	24.868198603016417	25.05110161992753
6	24.731546568591103	25.075014953557464	24.929557621865314	25.26388085598612
7	25.053542386538336	25.092347486072182	24.734543459239816	25.119566668149663
8	25.129546005052077	25.078722447143498	24.785027163569673	25.006704384234745
9	24.784718205770837	25.001204935415462	24.852719817294716	25.361357041518986
10-14	24.944449387769225	25.052893575160784	24.875663023436303	25.12699401363369
15-19	24.89436114942188	24.91559272935791	24.95150598389465	25.23854013732556
20-24	24.90869061213191	24.925874844903184	24.894577419881063	25.270857123083847
25-29	24.96190550340348	24.97872516597213	24.7965698269342	25.262799503690193
30-34	24.91327554586664	24.928494807037318	24.859387126593603	25.29884252050244
35-39	24.879036842599593	24.949083754751772	24.91944852268739	25.252430879961246
40-44	24.995890861275477	24.923464974072264	24.758061326887237	25.322582837765022
45-49	25.192900891281454	24.842629255584722	24.661641777026393	25.30282807610743
50-54	25.125739644970412	24.840169951505985	24.702523072968415	25.33156733055518
55-59	25.108203200308466	24.872190337777383	24.6772256083997	25.342380853514456
60-64	25.03673508228164	24.850977295309278	24.771680186659946	25.340607435749135
65-69	25.001705447049577	24.876336551437767	24.79235564255807	25.329602358954585
70-74	24.96962326921841	24.845644683701366	24.799066205948797	25.385665841131427
75-79	24.889646453411636	24.799233043160168	24.859844384135883	25.451276119292316
80-84	24.857193526221867	24.774874810299913	24.952525544630806	25.415406118847418
85-89	24.80298379083804	24.650383354836798	25.08404887959544	25.462583974729725
90-94	24.638806308940467	24.702427829878083	25.624253720548467	25.034512140632987
95-99	24.92334717966142	24.89872111138797	25.00626031340545	25.171671395545157
100-104	24.99572418482035	24.909528982677372	24.92440869493401	25.170338137568265
105-109	24.947506500705042	24.86987602103482	25.0242328402044	25.158384638055736
110-114	24.92299761590756	24.84074157124659	25.004035052499475	25.232225760346378
115-119	24.92700711126908	24.885629879177966	24.9739025954458	25.213460414107153
120-124	24.91958431214649	24.895240670451837	24.98131915773755	25.20385585966412
125-129	24.868750339933698	24.961158588051386	25.024988903892602	25.145102168122314
130-134	24.965185500674288	24.969163707572598	24.964313754467007	25.101337037286108
135-139	24.98144688264452	25.014217344354638	24.988188193301895	25.016147579698945
140-144	24.901138974834065	25.057916226734427	25.09622277975858	24.944722018672923
145-149	24.83154477803078	25.15488816419142	25.169079612619772	24.84448744515803
150-154	24.71728440307492	25.26461617979835	25.25452192775901	24.76357748936772
155-159	24.581989486235685	25.365105471377113	25.366189733785728	24.686715308601475
160-164	24.45218234760747	25.44000321070246	25.582523226580108	24.525291215109963
165-169	24.26930292724298	25.619982299870248	25.7299128897933	24.380801883093476
170-174	24.097246614213272	25.713935295209268	25.989729785612326	24.199088304965137
175-179	23.664877137563405	25.8813283182645	26.473703793700455	23.98009075047164
180	24.928331113759025	24.30486437232775	26.58022008831569	24.18658442559753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.5
18	2.0
19	3.0
20	10.0
21	24.5
22	52.5
23	98.0
24	188.0
25	345.0
26	617.5
27	1043.5
28	1657.0
29	2597.5
30	3821.5
31	5637.0
32	8096.5
33	11116.0
34	15304.0
35	20689.5
36	27083.5
37	34972.0
38	44638.0
39	56064.5
40	68661.5
41	82251.5
42	96843.0
43	111496.0
44	125666.0
45	138554.0
46	149861.5
47	159242.0
48	165121.0
49	167501.5
50	166515.0
51	161718.5
52	154626.0
53	145218.5
54	133696.5
55	121513.0
56	109617.5
57	98630.0
58	88799.0
59	80423.5
60	72633.0
61	65393.0
62	59101.5
63	52552.5
64	45777.0
65	39512.5
66	33219.0
67	27279.0
68	21958.0
69	17024.5
70	12864.0
71	9599.0
72	6933.0
73	4856.5
74	3310.0
75	2157.0
76	1345.0
77	837.0
78	495.0
79	262.5
80	159.0
81	84.0
82	38.5
83	22.0
84	12.0
85	5.0
86	1.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	975.0
92-93	2266.0
94-95	3430.0
96-97	4710.0
98-99	6207.0
100-101	7599.0
102-103	9225.0
104-105	10861.0
106-107	11870.0
108-109	13199.0
110-111	14770.0
112-113	16518.0
114-115	18609.0
116-117	20268.0
118-119	22471.0
120-121	24311.0
122-123	26483.0
124-125	28732.0
126-127	30807.0
128-129	33409.0
130-131	36180.0
132-133	49413.0
134-135	57619.0
136-137	60264.0
138-139	63758.0
140-141	65322.0
142-143	69056.0
144-145	71793.0
146-147	75543.0
148-149	79330.0
150-151	80523.0
152-153	80826.0
154-155	84900.0
156-157	88817.0
158-159	93516.0
160-161	98759.0
162-163	100342.0
164-165	103300.0
166-167	105653.0
168-169	107923.0
170-171	109966.0
172-173	114505.0
174-175	119319.0
176-177	121913.0
178-179	320748.0
180-181	570680.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.98168836085476
#Duplication Level	Percentage of total
1	92.54010994512166
2	6.180076596539803
3	0.7632193369469507
4	0.19246552588856528
5	0.09772173278444046
6	0.06363379099713147
7	0.052562225553943076
8	0.03668659982395065
9	0.019185263863764606
>10	0.0543389824798131
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	3.0895779883634134E-5	0.0
10-14	0.0	3.707493586036096E-5	0.0	1.2358311953453653E-5	3.0895779883634134E-5	0.0
15-19	0.0	6.179155976726827E-5	0.0	5.5612403790541445E-5	3.0895779883634134E-5	0.0
20-24	0.0	6.179155976726827E-5	3.707493586036096E-5	9.26873396509024E-5	3.0895779883634134E-5	0.0
25-29	0.0	1.359414314879902E-4	6.179155976726827E-5	9.26873396509024E-5	3.0895779883634134E-5	0.0
30-34	0.0	1.853746793018048E-4	7.414987172072192E-5	1.1122480758108289E-4	3.0895779883634134E-5	0.0
35-39	4.9433247813814614E-5	3.460327346967023E-4	9.26873396509024E-5	1.2358311953453654E-4	3.0895779883634134E-5	0.0
40-44	1.359414314879902E-4	4.0164513848724376E-4	9.26873396509024E-5	1.2358311953453654E-4	3.707493586036096E-5	0.0
45-49	1.5447889941817069E-4	4.0164513848724376E-4	9.26873396509024E-5	1.4212058746471704E-4	8.650818367417557E-5	0.0
50-54	1.5447889941817069E-4	4.3254091837087796E-4	9.26873396509024E-5	1.7301636734835113E-4	9.26873396509024E-5	2.4716623906907307E-5
55-59	1.5447889941817069E-4	4.63436698254512E-4	9.26873396509024E-5	1.9773299125525846E-4	9.26873396509024E-5	6.179155976726827E-5
60-64	1.7301636734835113E-4	5.066907900915997E-4	1.1740396355780971E-4	2.1627045918543893E-4	1.359414314879902E-4	6.179155976726827E-5
65-69	2.1009130320871213E-4	5.746615058355948E-4	1.2976227551126336E-4	2.1627045918543893E-4	1.5447889941817069E-4	8.032902769744874E-5
70-74	4.7579501020796565E-4	6.179155976726827E-4	1.5447889941817069E-4	2.3480792711561943E-4	1.5447889941817069E-4	9.26873396509024E-5
75-79	0.0018784634169249554	6.549905335330437E-4	1.853746793018048E-4	2.780620189527072E-4	1.5447889941817069E-4	1.2976227551126336E-4
80-84	0.0021627045918543894	7.106029373235851E-4	1.853746793018048E-4	3.0895779883634137E-4	1.6683721137162433E-4	1.5447889941817069E-4
85-89	0.0021688837478311164	7.106029373235851E-4	1.853746793018048E-4	3.0895779883634137E-4	2.1627045918543893E-4	1.5447889941817069E-4
90-94	0.0021936003717380236	7.167820933003119E-4	1.853746793018048E-4	3.0895779883634137E-4	2.1627045918543893E-4	1.5447889941817069E-4
95-99	0.0021936003717380236	7.414987172072192E-4	2.039121472319853E-4	3.0895779883634137E-4	2.1627045918543893E-4	1.5447889941817069E-4
100-104	0.0021936003717380236	7.538570291606729E-4	3.0895779883634137E-4	3.21316110789795E-4	2.1627045918543893E-4	1.5447889941817069E-4
105-109	0.0021936003717380236	7.971111209977607E-4	3.0895779883634137E-4	3.4603273469670226E-4	2.1627045918543893E-4	1.5447889941817069E-4
110-114	0.0021936003717380236	8.341860568581216E-4	3.0895779883634137E-4	3.7692851458033646E-4	2.1627045918543893E-4	1.5447889941817069E-4
115-119	0.0021936003717380236	9.26873396509024E-4	3.0895779883634137E-4	4.0164513848724376E-4	2.1627045918543893E-4	1.5447889941817069E-4
120-124	0.002205958683691477	9.577691763926583E-4	3.2749526676652187E-4	4.078242944639706E-4	2.2244961516216578E-4	1.5447889941817069E-4
125-129	0.002255391931505292	9.577691763926583E-4	3.3985357871997547E-4	4.3254091837087786E-4	2.7188286297598037E-4	1.5447889941817069E-4
130-134	0.00231718349127256	9.577691763926583E-4	3.3985357871997547E-4	4.3254091837087786E-4	2.780620189527072E-4	1.5447889941817069E-4
135-139	0.00231718349127256	9.886649562762923E-4	3.3985357871997547E-4	4.3254091837087786E-4	2.780620189527072E-4	1.5447889941817069E-4
140-144	0.002323362647249287	9.886649562762923E-4	3.3985357871997547E-4	4.3254091837087786E-4	2.780620189527072E-4	1.5447889941817069E-4
145-149	0.002403691674946736	9.886649562762923E-4	3.3985357871997547E-4	4.5725754227778515E-4	2.780620189527072E-4	1.5447889941817069E-4
150-154	0.0025149164825278183	0.0010195607361599265	3.3985357871997547E-4	4.63436698254512E-4	2.780620189527072E-4	1.5447889941817069E-4
155-159	0.0025828871982718138	0.0010195607361599265	3.3985357871997547E-4	4.63436698254512E-4	2.780620189527072E-4	1.5447889941817069E-4
160-164	0.0026941120058528965	0.0010195607361599265	3.3985357871997547E-4	4.6961585423123885E-4	2.780620189527072E-4	1.5447889941817069E-4
165-169	0.0027620827215968915	0.0010195607361599265	3.3985357871997547E-4	4.943324781381462E-4	2.780620189527072E-4	1.5447889941817069E-4
>>END_MODULE
