##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479425
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6243454
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.06296915137038	41.0	40.0	41.0	38.0	41.0
2	40.17404725012789	41.0	40.0	41.0	38.0	41.0
3	40.21239285177724	41.0	40.0	41.0	38.0	41.0
4	40.225217323616064	41.0	40.0	41.0	38.0	41.0
5	40.21716168646393	41.0	40.0	41.0	38.0	41.0
6	40.227430041127874	41.0	40.0	41.0	38.0	41.0
7	40.21522189480374	41.0	40.0	41.0	38.0	41.0
8	40.189212733848926	41.0	40.0	41.0	38.0	41.0
9	40.17025415739429	41.0	40.0	41.0	38.0	41.0
10-14	40.10719127585468	41.0	40.0	41.0	38.0	41.0
15-19	39.95143896311241	41.0	40.0	41.0	38.0	41.0
20-24	39.732133175002176	41.0	39.6	41.0	37.2	41.0
25-29	39.498004726230064	40.2	39.0	41.0	36.2	41.0
30-34	39.26870978788344	40.2	38.4	41.0	35.2	41.0
35-39	38.737011884767625	40.0	37.2	41.0	35.0	41.0
40-44	38.18470077620496	39.2	35.6	41.0	35.0	41.0
45-49	37.57207452797762	37.8	35.0	40.6	34.8	41.0
50-54	36.87488182022323	36.4	35.0	39.4	34.0	41.0
55-59	36.225229656533074	35.2	35.0	38.0	34.0	40.6
60-64	35.51010648913246	35.0	34.6	36.6	33.4	39.2
65-69	35.3579411972924	35.0	35.0	36.0	33.6	38.4
70-74	35.15462623733593	35.0	35.0	35.4	33.0	37.0
75-79	35.042247416253886	35.0	35.0	35.0	33.0	37.0
80-84	34.99412437410446	35.0	35.0	35.0	33.0	37.0
85-89	35.75154963902993	36.2	35.6	36.2	33.2	38.0
90-94	39.8147477355556	41.0	40.0	41.0	37.0	41.0
95-99	39.738170919727686	41.0	40.0	41.0	37.0	41.0
100-104	39.59037047769558	41.0	39.2	41.0	36.2	41.0
105-109	39.393590656408875	40.4	39.0	41.0	35.4	41.0
110-114	39.136062677841004	40.0	38.2	41.0	35.0	41.0
115-119	38.782705865361216	40.0	37.8	41.0	35.0	41.0
120-124	38.62363155107669	40.0	36.8	41.0	35.0	41.0
125-129	37.841563271376856	39.0	35.2	40.6	33.8	41.0
130-134	37.57557570502013	38.2	35.0	40.8	34.0	41.0
135-139	37.134365493614226	36.8	35.0	40.0	34.0	41.0
140-144	36.48900409920603	35.6	35.0	39.0	34.0	41.0
145-149	35.85589825799272	35.0	35.0	37.0	33.6	39.8
150-154	35.32740422688981	35.0	35.0	36.0	33.0	38.2
155-159	34.933055408622714	35.0	35.0	35.2	33.0	36.8
160-164	34.67408663278619	35.0	35.0	35.0	33.0	36.0
165-169	34.51476312939546	35.0	34.8	35.0	33.0	36.0
170-174	34.421647057295345	35.0	34.0	35.0	33.0	35.0
175-176	33.81156440489825	34.5	33.5	35.0	31.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	30.0
30	326.0
31	2181.0
32	11785.0
33	47532.0
34	150274.0
35	435777.0
36	1211931.0
37	2114096.0
38	1835522.0
39	429005.0
40	4995.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.242654786917626	26.834697588866675	26.739573960182938	22.18307366403276
2	22.088254354080288	26.330457467933616	27.982107339943564	23.599180838042532
3	22.39595262494126	25.459529292599896	28.410748281319925	23.73376980113892
4	23.576997604210746	26.033106033935706	26.760764153944276	23.629132207909276
5	23.224292194673012	26.479717797232112	26.979953724332717	23.316036283762163
6	23.07089953733943	26.359880284214476	26.854926776108222	23.714293402337873
7	23.49590467071592	26.577804529351862	26.417188306344535	23.509102493587683
8	23.319255655603452	26.809711419352173	26.681096713453805	23.189936211590574
9	23.00023352458431	26.50787528826191	26.768179920921973	23.7237112662318
10-14	23.457884049438018	26.51562100081141	26.513484362982414	23.513010586768157
15-19	23.467301913331948	26.386794873478685	26.43094671635284	23.714956496836525
20-24	23.466882273818307	26.337786744324536	26.45397563592204	23.74135534593512
25-29	23.641599665826	26.35555255152036	26.250809247573535	23.752038535080104
30-34	23.64055216871943	26.27202186482034	26.193949695152714	23.893476271307517
35-39	23.592248777679792	26.281990705785613	26.24566145598254	23.88009906055206
40-44	23.801908366746996	26.27036573025124	26.088271011526633	23.839454891475135
45-49	23.887210508798496	26.121698021639943	26.02777885446101	23.963312615100552
50-54	23.83703315504527	26.20543051970912	26.09996646087246	23.857569864373147
55-59	23.857002870526475	26.22001219196938	26.038282655722295	23.88470228178185
60-64	23.809788620209265	26.167486778952803	26.058662400651944	23.964062200185985
65-69	23.752679206093294	26.207006570401575	26.10318903606882	23.937125187436315
70-74	23.788053856086712	26.243101975284834	26.05067323311744	23.918170935511014
75-79	23.737174967574038	26.18498350432309	26.047373777399496	24.030467750703377
80-84	23.696851134003712	26.250549775813198	26.077078488926166	23.97552060125693
85-89	23.83084427305783	26.330633652462243	26.070364256707908	23.76815781777202
90-94	22.83474166438993	26.182195955775644	27.338480027752126	23.644582352082306
95-99	23.258702378344662	26.59822011069562	26.63246787351673	23.51060963744299
100-104	23.363220261448813	26.383750497469805	26.52194735987442	23.731081881206958
105-109	23.380872722970476	26.368343331253303	26.510233823532353	23.740550122243867
110-114	23.502481663724932	26.31480823731363	26.37147071686037	23.81123938210107
115-119	23.530558361703918	26.279218247984204	26.273574670373893	23.91664871993798
120-124	23.55230424257664	26.25620376525543	26.292174166641523	23.899317825526406
125-129	23.6222200150456	26.293757116512275	26.224155334374803	23.859867534067327
130-134	23.744436187934532	26.168794141981333	26.102210062534354	23.98455960754978
135-139	23.77119212265203	26.232074371177834	26.157123556480943	23.839609949689194
140-144	23.79189130374108	26.29969479926219	26.150445056137674	23.757968840859057
145-149	23.719995363884966	26.29527586649285	26.18229037192866	23.802438397693525
150-154	23.622448795879876	26.39190782038947	26.298065477974724	23.687577905755933
155-159	23.599231143113116	26.503685304068114	26.27232506667379	23.624758486144977
160-164	23.50892495758863	26.510561832028323	26.34311684566629	23.637396364716757
165-169	23.392206241096254	26.652987054973927	26.445078058743597	23.50972864518622
170-174	23.33903648981957	26.794050360943842	26.51437293494207	23.352540214294518
175-176	23.383359473025628	26.708462820832384	26.330665340933507	23.577512365208484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	3.0
4	5.0
5	6.0
6	8.0
7	7.5
8	10.0
9	21.0
10	32.0
11	50.5
12	85.5
13	162.0
14	292.5
15	467.5
16	753.5
17	1186.0
18	1845.5
19	2806.0
20	4150.0
21	5961.0
22	8298.0
23	11502.5
24	15439.5
25	20360.5
26	26388.5
27	33100.5
28	40966.5
29	49728.5
30	59121.5
31	69312.0
32	80151.0
33	92133.0
34	104899.5
35	118543.5
36	132844.5
37	147895.5
38	163616.5
39	179124.0
40	194035.5
41	207974.5
42	219492.5
43	227711.5
44	233970.0
45	237330.5
46	237333.0
47	234178.5
48	228290.5
49	220669.0
50	211408.5
51	201086.5
52	191131.0
53	180952.5
54	170559.0
55	161622.0
56	153055.5
57	144463.5
58	136758.0
59	129050.0
60	121592.5
61	114215.5
62	106319.0
63	97868.0
64	88935.0
65	79775.0
66	69877.5
67	60022.0
68	50583.5
69	41621.0
70	33232.0
71	25626.0
72	19378.5
73	14304.5
74	10048.0
75	6850.0
76	4540.5
77	2883.0
78	1786.0
79	1037.0
80	590.0
81	334.5
82	176.0
83	80.5
84	40.5
85	22.5
86	12.5
87	5.5
88	1.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1063.0
92-93	2128.0
94-95	3030.0
96-97	3922.0
98-99	4991.0
100-101	7215.0
102-103	7423.0
104-105	8630.0
106-107	8828.0
108-109	10597.0
110-111	10743.0
112-113	11314.0
114-115	11513.0
116-117	13889.0
118-119	13246.0
120-121	12694.0
122-123	14678.0
124-125	14589.0
126-127	15301.0
128-129	16256.0
130-131	16993.0
132-133	48880.0
134-135	72599.0
136-137	66853.0
138-139	62148.0
140-141	62962.0
142-143	70349.0
144-145	64725.0
146-147	66207.0
148-149	66670.0
150-151	65229.0
152-153	65819.0
154-155	69039.0
156-157	73455.0
158-159	70719.0
160-161	71999.0
162-163	78515.0
164-165	76977.0
166-167	82001.0
168-169	82364.0
170-171	86212.0
172-173	89725.0
174-175	255910.0
176-177	4285054.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.53601663161346
#Duplication Level	Percentage of total
1	95.30787864844837
2	4.104052822448037
3	0.41645484460258303
4	0.10136670660997141
5	0.03612391546333564
6	0.018726493361714072
7	0.006291916384842696
8	0.0010569878212277988
9	0.0021307319934256086
>10	0.005916932866467419
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.601677532980943E-5	1.601677532980943E-5	0.0	0.0	0.0	0.0
2	1.601677532980943E-5	1.601677532980943E-5	0.0	0.0	0.0	0.0
3	4.8050325989428284E-5	3.203355065961886E-5	0.0	0.0	0.0	0.0
4	4.8050325989428284E-5	6.406710131923772E-5	0.0	0.0	0.0	0.0
5	8.008387664904715E-5	6.406710131923772E-5	1.601677532980943E-5	0.0	0.0	0.0
6	8.008387664904715E-5	6.406710131923772E-5	6.406710131923772E-5	0.0	0.0	0.0
7	8.008387664904715E-5	6.406710131923772E-5	6.406710131923772E-5	0.0	0.0	0.0
8	8.008387664904715E-5	6.406710131923772E-5	6.406710131923772E-5	0.0	0.0	0.0
9	9.610065197885657E-5	6.406710131923772E-5	8.008387664904715E-5	0.0	0.0	0.0
10-14	9.610065197885657E-5	7.367716651712337E-5	8.008387664904715E-5	2.24234854617332E-5	3.203355065961886E-6	0.0
15-19	1.601677532980943E-4	1.2172749250655168E-4	9.930400704481846E-5	5.7660391187313947E-5	1.601677532980943E-5	0.0
20-24	2.050147242215607E-4	1.6657446343001808E-4	1.2493084757251354E-4	6.406710131923772E-5	3.203355065961886E-5	0.0
25-29	2.402516299471414E-4	1.7618452862790373E-4	1.4735433303424676E-4	7.688052158308526E-5	3.203355065961886E-5	0.0
30-34	3.0111537620041725E-4	2.082180792875226E-4	1.7618452862790373E-4	8.96939418469328E-5	3.203355065961886E-5	0.0
35-39	4.548764193665878E-4	2.3064156474925579E-4	2.082180792875226E-4	1.4094762290232297E-4	4.484697092346639E-5	3.203355065961886E-6
40-44	6.502810783902628E-4	2.498616951450271E-4	2.498616951450271E-4	1.7298117356194187E-4	5.445703612135207E-5	3.523690572558074E-5
45-49	7.527884405010431E-4	2.5626840527695087E-4	2.690818255407984E-4	1.9540465902367503E-4	6.406710131923772E-5	6.0863746253275825E-5
50-54	8.809226431395187E-4	2.7228518060676033E-4	3.011153762004173E-4	2.1782814448540822E-4	9.289729691289468E-5	8.008387664904715E-5
55-59	9.578031647226037E-4	3.0431873126637914E-4	3.7158918765157876E-4	2.53065050210989E-4	1.313375577044373E-4	8.008387664904715E-5
60-64	0.0010987507876249268	3.203355065961886E-4	4.0041938324523573E-4	2.8509860087060784E-4	1.793878836938656E-4	9.930400704481846E-5
65-69	0.001175631309208012	3.5877576738773125E-4	4.0041938324523573E-4	2.947086660684935E-4	2.274382096832939E-4	1.2493084757251354E-4
70-74	0.0036742482606582833	3.9080931804735006E-4	4.164361585750452E-4	3.427589920579218E-4	2.402516299471414E-4	1.2813420263847543E-4
75-79	0.020652030110256278	4.164361585750452E-4	4.548764193665878E-4	3.5236905725580746E-4	2.466583400790652E-4	1.2813420263847543E-4
80-84	0.02478756150041307	4.388596440367784E-4	4.644864845644735E-4	3.844026079154263E-4	3.3314892686003617E-4	1.2813420263847543E-4
85-89	0.02485483195679827	5.157401656198636E-4	4.805032598942828E-4	4.036227383111976E-4	4.420629991027402E-4	1.2813420263847543E-4
90-94	0.02491569570305155	5.573837814773681E-4	5.253502308177492E-4	4.48469709234664E-4	4.48469709234664E-4	1.2813420263847543E-4
95-99	0.024954135963843087	5.669938466752539E-4	5.541804264114062E-4	4.869099700262066E-4	4.48469709234664E-4	1.2813420263847543E-4
100-104	0.025027813130360215	6.022307524008346E-4	5.862139770710251E-4	5.221468757517874E-4	4.48469709234664E-4	1.2813420263847543E-4
105-109	0.025098286941811376	6.310609479944914E-4	5.926206872029489E-4	5.349602960156349E-4	4.5167306430062595E-4	1.2813420263847543E-4
110-114	0.025162354043130615	6.727045638519962E-4	6.18247527730644E-4	5.798072669391014E-4	4.6768983963043536E-4	1.2813420263847543E-4
115-119	0.025210404369120042	6.887213391818055E-4	6.278575929285296E-4	6.118408175987202E-4	4.805032598942828E-4	1.2813420263847543E-4
120-124	0.02538979225281391	7.3036495503931E-4	6.502810783902628E-4	6.470777233243011E-4	4.8370661496024476E-4	1.2813420263847543E-4
125-129	0.025658874078354703	7.816186360947001E-4	6.72704563851996E-4	7.367716651712336E-4	4.965200352240923E-4	1.313375577044373E-4
130-134	0.026001633070412627	7.84821991160662E-4	7.047381145116149E-4	7.688052158308525E-4	4.997233902900542E-4	1.4415097796828487E-4
135-139	0.02624508805542573	8.008387664904715E-4	7.33568310105272E-4	7.784152810287382E-4	5.125368105539017E-4	1.4415097796828487E-4
140-144	0.02646291619991114	8.232622519522047E-4	7.367716651712337E-4	7.880253462266239E-4	5.125368105539017E-4	1.4415097796828487E-4
145-149	0.026607067177879425	8.520924475458616E-4	7.495850854350812E-4	8.520924475458616E-4	5.317569409496731E-4	1.4415097796828487E-4
150-154	0.02687294564835426	8.905327083374043E-4	7.784152810287382E-4	8.745159330075948E-4	5.477737162794824E-4	1.4415097796828487E-4
155-159	0.027154840894158906	8.96939418469328E-4	7.912287012925859E-4	8.905327083374043E-4	5.6058713654333E-4	1.4415097796828487E-4
160-164	0.02743353278489759	9.065494836672138E-4	8.008387664904715E-4	9.065494836672138E-4	5.734005568071776E-4	1.4415097796828487E-4
165	0.027580887117931836	9.129561937991375E-4	8.168555418202809E-4	9.129561937991375E-4	5.766039118731395E-4	1.4415097796828487E-4
>>END_MODULE
