##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479446
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7338122
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.2754218313623	39.0	39.0	39.0	37.0	39.0
2	40.070487789655175	41.0	40.0	41.0	38.0	41.0
3	40.118063041197736	41.0	40.0	41.0	38.0	41.0
4	40.14656965365253	41.0	40.0	41.0	38.0	41.0
5	40.15943438934376	41.0	40.0	41.0	38.0	41.0
6	40.14935865061933	41.0	40.0	41.0	38.0	41.0
7	40.16281195651967	41.0	40.0	41.0	38.0	41.0
8	40.12869927755357	41.0	40.0	41.0	38.0	41.0
9	40.09643979753948	41.0	40.0	41.0	38.0	41.0
10-14	40.0248556238231	41.0	40.0	41.0	38.0	41.0
15-19	39.8680247343939	41.0	40.0	41.0	38.0	41.0
20-24	39.63300130469349	40.6	39.4	41.0	36.8	41.0
25-29	39.438612140817504	40.0	39.0	41.0	35.8	41.0
30-34	39.15887746210815	40.0	38.2	41.0	35.0	41.0
35-39	38.613909607935106	40.0	37.0	41.0	35.0	41.0
40-44	37.899425929413546	38.8	35.2	41.0	34.2	41.0
45-49	37.33900229513764	37.4	35.0	40.0	34.0	41.0
50-54	36.751012725054174	36.2	35.0	39.4	34.0	41.0
55-59	36.13746296395726	35.0	35.0	38.0	33.6	40.6
60-64	35.53391061091653	35.0	34.8	36.8	32.8	39.4
65-69	35.31073983234403	35.0	34.8	36.0	33.0	39.0
70-74	35.18568513851364	35.0	35.0	35.4	33.0	37.8
75-79	35.084633125478156	35.0	35.0	35.0	33.0	37.4
80-84	35.04955276022939	35.0	35.0	35.0	33.0	38.0
85-89	34.846125643591094	35.0	34.6	35.0	32.2	38.6
90-94	39.42517841034961	40.6	39.8	40.6	36.8	40.8
95-99	39.642818692567445	41.0	39.6	41.0	36.6	41.0
100-104	39.4913281719125	41.0	39.0	41.0	36.0	41.0
105-109	39.29782626380348	40.2	38.8	41.0	35.0	41.0
110-114	39.04228013896257	40.0	38.0	41.0	35.0	41.0
115-119	38.698599133182014	40.0	37.6	41.0	34.8	41.0
120-124	38.52516721581923	40.0	36.6	41.0	34.6	41.0
125-129	37.86603612363507	39.0	35.2	40.6	33.8	41.0
130-134	37.42317658165187	38.0	35.0	40.4	33.8	41.0
135-139	36.8057611037858	36.6	35.0	39.8	33.0	41.0
140-144	36.15633686864523	35.2	35.0	38.8	33.0	41.0
145-149	35.58370639534159	35.0	35.0	37.0	33.0	39.6
150-154	35.316443206684184	35.0	35.0	36.0	33.0	38.6
155-159	34.94549833824793	35.0	35.0	35.2	33.0	37.0
160-164	34.67129474181672	35.0	35.0	35.0	33.0	36.0
165-169	34.50071868737823	35.0	34.6	35.0	33.0	36.0
170-174	34.39551053855215	35.0	34.0	35.0	33.0	35.0
175-178	34.02885317458504	34.75	33.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	30.0
30	312.0
31	2675.0
32	14797.0
33	62748.0
34	205816.0
35	596120.0
36	1593311.0
37	2632013.0
38	1844333.0
39	382096.0
40	3870.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.786276652255168	25.11916536683364	26.345691717853697	24.748866263057494
2	24.98922748899514	25.59731222784249	26.017760402457196	23.395699880705173
3	22.70236172143227	25.34648510886028	27.393262199783543	24.55789096992391
4	23.54396669883657	24.43450790270317	27.449107550951048	24.57241784750921
5	24.395887667171518	25.188529163183716	25.933283747531043	24.482299422113723
6	23.969102721377485	25.524350780758347	26.206391771627676	24.300154726236496
7	24.174100675895005	25.344659028563438	25.780179179359514	24.70106111618204
8	24.467241073397254	25.6638960213526	25.48034769659049	24.388515208659655
9	24.22087013543792	25.775027997626644	25.790249875921933	24.213851991013506
10-14	24.40164390834603	25.444970797705462	25.54341560415594	24.609969689792564
15-19	24.460086654323817	25.321121671185082	25.51489059462353	24.703901079867574
20-24	24.54495032925318	25.223622610798785	25.432624314504448	24.798802745443588
25-29	24.674711050047954	25.1284947293054	25.23116132438245	24.965632896264196
30-34	24.64424548951353	25.17818319183028	25.25864247010339	24.918928848552806
35-39	24.6467747469993	25.189900086152832	25.232605835662042	24.930719331185827
40-44	24.775813212154283	25.10649182447498	25.054216869111745	25.063478094258997
45-49	24.932417313312587	25.02003918713807	25.029714687218334	25.017828812331
50-54	24.963250815399363	25.09920930723147	25.010704373680348	24.926835503688817
55-59	24.934578629246012	25.003113875730058	24.99703330089088	25.065274194133046
60-64	24.854432782665647	25.076110754222945	25.07973020890086	24.989726254210545
65-69	24.855825509578608	25.10210923176257	25.053690848966536	24.988374409692288
70-74	24.869632857017095	25.054146006294253	25.014247514554818	25.061973622133838
75-79	24.77895025457467	25.05602932194368	25.095309126776577	25.069711296705073
80-84	24.81624044953191	25.11427855791986	25.03903859870414	25.030442393844094
85-89	24.81456699684197	25.023928465621044	24.964719856115774	25.196784681421214
90-94	23.906185972763623	25.173462992603262	26.416806749905202	24.50354428472791
95-99	24.18142822604939	25.541912438532172	25.66812311242797	24.60853622299047
100-104	24.35247451535965	25.45575774505956	25.521669473799953	24.670098265780844
105-109	24.453127437225866	25.302890693272413	25.407932634946416	24.8360492345553
110-114	24.49360191315853	25.17970565562378	25.388824981561754	24.93786744965594
115-119	24.6057944207368	25.255200525965364	25.193065664942328	24.945939388355512
120-124	24.644881554373587	25.184969863162543	25.14340306686393	25.02674551559994
125-129	24.61036156039744	25.18177850653433	25.19715380128274	25.010706131785494
130-134	24.758284843625177	25.217598858037917	25.044538160790275	24.97957813754664
135-139	24.883713346314444	25.12609918170776	24.963844112887163	25.026343359090635
140-144	24.801488080621855	25.23195895942949	25.034882227679628	24.931670732269026
145-149	24.740020249578773	25.321252127690485	25.04158315817437	24.897144464556373
150-154	24.701264297189564	25.364943656718573	25.088653667609996	24.845138378481863
155-159	24.590350424069776	25.403491821552183	25.24054019517784	24.7656175592002
160-164	24.58774270752682	25.553366246603115	25.214592687061543	24.64429835880852
165-169	24.48987096493374	25.53390753962834	25.26304196754086	24.71317952789706
170-174	24.3922955058747	25.674861479847305	25.369761569836726	24.563081444441274
175-178	24.41255716361632	25.75450931375193	25.233443212340372	24.599490310291376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	3.0
10	5.0
11	8.0
12	18.0
13	30.5
14	61.0
15	108.5
16	199.5
17	364.5
18	610.0
19	1013.0
20	1699.5
21	2848.5
22	4444.0
23	6760.5
24	10049.0
25	14259.0
26	19814.0
27	26482.0
28	34119.0
29	43093.0
30	53110.5
31	64170.0
32	75662.5
33	87615.0
34	100102.5
35	112672.5
36	125469.5
37	138746.5
38	151884.0
39	164482.5
40	176974.5
41	189303.5
42	201172.5
43	212484.0
44	222248.0
45	231026.5
46	239692.5
47	246865.5
48	253722.5
49	259379.0
50	262553.5
51	265070.5
52	266298.5
53	265588.5
54	262937.5
55	257608.5
56	250189.0
57	240132.0
58	226797.5
59	211204.0
60	193567.0
61	174551.5
62	155915.0
63	137577.5
64	119996.0
65	103755.0
66	88950.5
67	76064.0
68	64150.0
69	53242.0
70	43889.5
71	35747.5
72	28543.0
73	22342.0
74	17148.5
75	12831.0
76	9425.0
77	6818.0
78	4776.5
79	3230.5
80	2119.0
81	1369.0
82	882.5
83	551.5
84	317.5
85	171.0
86	88.5
87	46.0
88	17.5
89	8.5
90	4.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1379.0
92-93	3394.0
94-95	6106.0
96-97	7021.0
98-99	8463.0
100-101	10266.0
102-103	11276.0
104-105	13042.0
106-107	14074.0
108-109	14450.0
110-111	15719.0
112-113	17130.0
114-115	16929.0
116-117	18242.0
118-119	19114.0
120-121	19671.0
122-123	20199.0
124-125	21831.0
126-127	23356.0
128-129	23785.0
130-131	25323.0
132-133	27787.0
134-135	107668.0
136-137	117299.0
138-139	107622.0
140-141	111067.0
142-143	108841.0
144-145	123614.0
146-147	123908.0
148-149	90876.0
150-151	96876.0
152-153	88970.0
154-155	89308.0
156-157	88903.0
158-159	85444.0
160-161	90637.0
162-163	92255.0
164-165	96669.0
166-167	97494.0
168-169	102441.0
170-171	104031.0
172-173	112401.0
174-175	117502.0
176-177	336574.0
178-179	4509165.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.26958900654095
#Duplication Level	Percentage of total
1	87.32675005861012
2	10.589196569866683
3	1.6314790535486376
4	0.33648203917938163
5	0.06775588569688175
6	0.020671947307700306
7	0.01248745975689519
8	0.007333740148966518
9	0.00210952781944915
>10	0.005733718065303448
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.539225431247941E-5	1.3627464901782773E-5	1.3627464901782773E-5	0.0	1.3627464901782773E-5	0.0
2	2.8617676293743823E-4	2.7254929803565546E-5	1.3627464901782773E-5	0.0	1.3627464901782773E-5	0.0
3	2.8617676293743823E-4	2.7254929803565546E-5	1.3627464901782773E-5	0.0	2.7254929803565546E-5	0.0
4	2.8617676293743823E-4	2.7254929803565546E-5	2.7254929803565546E-5	0.0	2.7254929803565546E-5	0.0
5	2.8617676293743823E-4	2.7254929803565546E-5	2.7254929803565546E-5	0.0	2.7254929803565546E-5	0.0
6	2.99804227839221E-4	4.088239470534831E-5	4.088239470534831E-5	1.3627464901782773E-5	2.7254929803565546E-5	0.0
7	3.270591576427865E-4	4.088239470534831E-5	4.088239470534831E-5	1.3627464901782773E-5	2.7254929803565546E-5	0.0
8	3.406866225445693E-4	5.450985960713109E-5	4.088239470534831E-5	1.3627464901782773E-5	4.088239470534831E-5	0.0
9	3.406866225445693E-4	5.450985960713109E-5	4.088239470534831E-5	5.450985960713109E-5	5.450985960713109E-5	0.0
10-14	3.7066704532849143E-4	6.813732450891386E-5	6.54118315285573E-5	6.813732450891386E-5	5.45098596071311E-5	0.0
15-19	3.8156901724991765E-4	6.813732450891386E-5	1.2809817007675806E-4	8.99412683517663E-5	5.7235352587487645E-5	0.0
20-24	3.979219751320569E-4	1.0356873325354906E-4	1.7715704372317604E-4	1.0356873325354906E-4	7.903929643034009E-5	0.0
25-29	4.088239470534832E-4	1.253726770964015E-4	1.9351000160531536E-4	1.090197192142622E-4	8.176478941069663E-5	0.0
30-34	4.2517690493562246E-4	1.499021139196105E-4	2.1803943842852434E-4	1.199216911356884E-4	8.99412683517663E-5	0.0
35-39	5.205691592481019E-4	1.5807859286068017E-4	2.616473261142292E-4	1.362746490178277E-4	1.1447070517497529E-4	0.0
40-44	6.4866732932486E-4	1.6352957882139325E-4	2.970787348588644E-4	1.444511279588974E-4	1.8260802968388915E-4	1.9078450862495883E-5
45-49	7.794909923819745E-4	1.8260802968388915E-4	3.1615718572136034E-4	1.689805647821064E-4	2.2349042438923747E-4	2.725492980356555E-5
50-54	8.612557817926712E-4	2.2894141034995058E-4	3.4341211552492586E-4	1.7715704372317604E-4	2.7527479101601196E-4	2.725492980356555E-5
55-59	9.43020571203368E-4	2.5892183313387266E-4	3.8429451023027424E-4	1.7715704372317604E-4	3.652160593677783E-4	2.725492980356555E-5
60-64	0.0010629422623390562	2.725492980356554E-4	3.979219751320569E-4	1.8805901564460225E-4	4.197259189749093E-4	3.8156901724991766E-5
65-69	0.001180138460494388	3.0252972081957753E-4	4.687847926213274E-4	2.044119735267416E-4	4.6060831368025765E-4	5.723535258748764E-5
70-74	0.0015071976181371745	3.2978465062314305E-4	5.369221171302411E-4	2.180394384285244E-4	5.205691592481019E-4	7.358831046962698E-5
75-79	0.006576614561600367	3.5158859446599554E-4	5.723535258748765E-4	2.2349042438923747E-4	5.614515539534503E-4	8.176478941069663E-5
80-84	0.008699773593298123	4.006474681124135E-4	6.05059441639155E-4	2.452943682320899E-4	6.323143714427207E-4	9.266676133212286E-5
85-89	0.008721577537140974	4.3880436983740527E-4	6.404908503837903E-4	2.616473261142292E-4	7.549615555587656E-4	9.539225431247941E-5
90-94	0.008759734438865966	4.933142294445364E-4	6.54118315285573E-4	2.943532418785079E-4	7.685890204605485E-4	9.539225431247941E-5
95-99	0.008789714861649887	5.069416943463192E-4	6.922752170105649E-4	3.0525521379993413E-4	7.958439502641138E-4	9.539225431247941E-5
100-104	0.008830597256355236	5.45098596071311E-4	7.413340906569827E-4	3.54314087446352E-4	8.040204292051837E-4	9.539225431247941E-5
105-109	0.00889055810192308	5.750790188552331E-4	7.794909923819748E-4	3.7339253830884797E-4	8.040204292051837E-4	9.539225431247941E-5
110-114	0.008966871905373064	6.023339486587985E-4	7.958439502641138E-4	3.8156901724991765E-4	8.040204292051837E-4	9.539225431247941E-5
115-119	0.008983224863255203	6.159614135605813E-4	8.094714151658966E-4	3.979219751320569E-4	8.25824373048036E-4	9.539225431247941E-5
120-124	0.009084068103528397	6.268633854820075E-4	8.503538098712451E-4	4.333533838766922E-4	8.639812747730277E-4	9.539225431247941E-5
125-129	0.009179460357840875	6.268633854820075E-4	8.694322607337409E-4	4.687847926213274E-4	9.184911343801588E-4	1.0356873325354906E-4
130-134	0.009269401626192641	6.268633854820075E-4	9.048636694783761E-4	4.824122575231101E-4	9.457460641837244E-4	1.090197192142622E-4
135-139	0.009378421345406904	6.268633854820075E-4	9.29393106301585E-4	5.151181732873888E-4	9.648245150462203E-4	1.090197192142622E-4
140-144	0.009555578389130081	6.268633854820075E-4	9.620990220658637E-4	5.314711311695281E-4	9.920794448497858E-4	1.090197192142622E-4
145-149	0.009686402052187196	6.323143714427207E-4	0.0010166088816729948	5.669025399141633E-4	0.0010057069097515687	1.090197192142622E-4
150-154	0.009795421771401455	6.895497240302082E-4	0.0010711187412801259	5.723535258748765E-4	0.0010193343746533515	1.090197192142622E-4
155-159	0.00986355909591037	7.222556397944869E-4	0.0010956481781033349	5.750790188552331E-4	0.0010438638114765603	1.090197192142622E-4
160-164	0.009948049378301424	7.277066257551999E-4	0.0011147266289658307	5.914319767373723E-4	0.0010520402904176302	1.253726770964015E-4
165-167	0.010070696562417469	7.358831046962697E-4	0.0011537920283509412	6.132359205802247E-4	0.0010856547038420275	1.6807206712198753E-4
>>END_MODULE
