##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479447
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7384568
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.32050703575348	39.0	39.0	39.0	37.0	39.0
2	40.08728811218205	41.0	40.0	41.0	38.0	41.0
3	40.11825525880458	41.0	40.0	41.0	38.0	41.0
4	40.1379304788039	41.0	40.0	41.0	38.0	41.0
5	40.146166031648704	41.0	40.0	41.0	38.0	41.0
6	40.14225639197851	41.0	40.0	41.0	38.0	41.0
7	40.13974453211075	41.0	40.0	41.0	38.0	41.0
8	40.11116872374931	41.0	40.0	41.0	38.0	41.0
9	40.080873248103345	41.0	40.0	41.0	38.0	41.0
10-14	40.01501999846165	41.0	40.0	41.0	38.0	41.0
15-19	39.85604168043411	41.0	40.0	41.0	38.0	41.0
20-24	39.62152356102619	40.6	39.2	41.0	36.8	41.0
25-29	39.435725664656346	40.0	39.0	41.0	36.0	41.0
30-34	39.15349992037449	40.0	38.2	41.0	35.0	41.0
35-39	38.61522458727443	40.0	37.0	41.0	35.0	41.0
40-44	37.901444580102726	38.8	35.2	41.0	34.2	41.0
45-49	37.331284863244534	37.4	35.0	40.0	34.0	41.0
50-54	36.72561165392478	36.2	35.0	39.4	34.0	41.0
55-59	36.10710229765641	35.0	35.0	38.0	33.4	40.6
60-64	35.49044515535642	35.0	34.8	36.6	32.8	39.2
65-69	35.259579734386634	35.0	34.8	36.0	33.0	38.8
70-74	35.13507910009089	35.0	35.0	35.2	33.0	37.4
75-79	35.024981258213074	35.0	35.0	35.0	33.0	37.0
80-84	34.99708202294298	35.0	35.0	35.0	33.0	37.4
85-89	34.78727327042016	35.0	34.6	35.0	32.2	38.2
90-94	39.42152285417745	40.6	39.8	40.6	36.8	40.6
95-99	39.641871862713444	41.0	39.6	41.0	36.6	41.0
100-104	39.4939589842798	41.0	39.0	41.0	36.0	41.0
105-109	39.30767773286381	40.2	38.8	41.0	35.2	41.0
110-114	39.05514562522836	40.0	38.0	41.0	35.0	41.0
115-119	38.7155229212794	40.0	37.6	41.0	34.8	41.0
120-124	38.55119224103171	40.0	36.8	41.0	34.6	41.0
125-129	37.88157973238883	39.0	35.4	40.6	33.6	41.0
130-134	37.44415391045693	38.2	35.0	40.6	33.6	41.0
135-139	36.822100189739146	36.6	35.0	39.8	33.0	41.0
140-144	36.16580877454814	35.2	35.0	38.8	33.0	41.0
145-149	35.59500567632257	35.0	35.0	37.0	33.0	39.6
150-154	35.31903341635966	35.0	35.0	36.0	33.0	38.6
155-159	34.94318380651485	35.0	35.0	35.2	33.0	37.0
160-164	34.672928400063945	35.0	35.0	35.0	33.0	36.0
165-169	34.499573492901604	35.0	34.6	35.0	33.0	36.0
170-174	34.39811404376881	35.0	34.0	35.0	33.0	35.0
175-178	34.029537879982485	34.75	33.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	29.0
30	375.0
31	2896.0
32	16045.0
33	66187.0
34	216493.0
35	613664.0
36	1607659.0
37	2644005.0
38	1839886.0
39	374518.0
40	2811.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.80221835590112	25.120941401040657	26.403778799247295	24.67306144381093
2	24.96603728207256	25.58218706903369	26.080672559315587	23.371103089578156
3	22.679105940929787	25.33862508951099	27.43408957707479	24.548179392484435
4	23.474386043977116	24.42262025347996	27.511561949189172	24.591431753353753
5	24.373490771565784	25.177559472673284	25.938335187650786	24.510614568110146
6	23.94520573173678	25.52456690763766	26.217010934153496	24.31321642647207
7	24.148318493377	25.35499706956453	25.82849802452899	24.66818641252948
8	24.43663596841413	25.650505215741802	25.509427227158042	24.40343158868603
9	24.17373636480834	25.806506216748225	25.853347142310827	24.166410276132606
10-14	24.36670364468172	25.460048576978366	25.57347972149488	24.599768056845033
15-19	24.433375114156984	25.33444068766108	25.556522737687565	24.67566146049437
20-24	24.51963879268225	25.246289288689606	25.47167823493534	24.762393683692803
25-29	24.654268198220937	25.13547170261009	25.26010458567109	24.95015551349788
30-34	24.61431460851874	25.18569535821188	25.302807151345885	24.897182881923495
35-39	24.610728752176158	25.22304893123064	25.27075652902106	24.89546578757214
40-44	24.73040535343435	25.11889388790245	25.091921423162468	25.058779335500738
45-49	24.92896808587855	25.03878087384394	25.045205081732608	24.9870459585449
50-54	24.944059016045355	25.11053320925476	25.03643273377671	24.908975040923178
55-59	24.92001157007424	25.000495628180282	25.02124430298428	25.0582484987612
60-64	24.84055668523873	25.099196053174676	25.086358470800185	24.973888790786408
65-69	24.8460952624446	25.099854182397667	25.083958872069427	24.9700916830883
70-74	24.846864434046786	25.089410782052518	25.027598093754435	25.03612669014626
75-79	24.754937594182895	25.069935032083123	25.117875548034768	25.05725182569922
80-84	24.780691301102515	25.12515288639769	25.07298192663403	25.021173885865768
85-89	24.787202176213963	25.035111058629294	24.993424124471467	25.18426264068528
90-94	23.887936125112702	25.211727842119807	26.43749081302202	24.462845219745468
95-99	24.169981297279065	25.567449169815802	25.684159400922567	24.578410131982565
100-104	24.342786450284482	25.48605185946186	25.542558887674833	24.628602802578826
105-109	24.42873367918311	25.32639175379948	25.440625487326923	24.804249079690486
110-114	24.45219308612165	25.213060652154162	25.423695964822556	24.91105029690163
115-119	24.58155870577975	25.27207688584316	25.22850206400584	24.917862344371247
120-124	24.61015290254729	25.207859954866912	25.188426687631143	24.993560454954654
125-129	24.575411888982128	25.21974223315455	25.22483923860087	24.98000663926245
130-134	24.738264868097367	25.244738940990853	25.065588986445714	24.951407204466065
135-139	24.854126698873007	25.159447391799834	24.991603304248645	24.994822605078514
140-144	24.769034899176187	25.262616169699882	25.056688324131056	24.911660606992882
145-149	24.71485312900391	25.36772140564344	25.061358689391472	24.856066775961178
150-154	24.676583057909745	25.404165333314015	25.12279488596894	24.796456722807296
155-159	24.56190911769959	25.43227109493537	25.264186023622415	24.741633763742627
160-164	24.55969767441697	25.590843256431068	25.232499564029244	24.61695950512272
165-169	24.45185864204059	25.541440020052253	25.308803928103657	24.6978974098035
170-174	24.373847624592113	25.693077478680216	25.399519026610058	24.53355587011762
175-178	24.382974462327972	25.784525735563314	25.275145637496223	24.557354164612494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.5
10	2.5
11	6.5
12	19.5
13	35.5
14	62.0
15	108.5
16	197.5
17	362.5
18	642.5
19	1117.0
20	1841.5
21	2894.5
22	4479.0
23	6964.0
24	10369.5
25	14688.5
26	20206.5
27	27074.0
28	35026.5
29	44237.0
30	54351.0
31	65163.5
32	76974.0
33	89201.0
34	101253.0
35	114096.5
36	127046.5
37	139716.0
38	152781.5
39	165811.0
40	178268.5
41	190504.0
42	202570.0
43	213660.0
44	223902.0
45	233533.5
46	242272.0
47	249554.0
48	255830.5
49	260104.0
50	263787.5
51	266671.0
52	267001.0
53	266458.0
54	264341.5
55	258825.0
56	251100.0
57	240744.5
58	227689.0
59	212151.5
60	193752.0
61	174814.0
62	156117.5
63	137764.5
64	120414.0
65	104240.0
66	89337.5
67	75990.0
68	64147.5
69	53537.5
70	44082.5
71	35891.0
72	28423.5
73	21996.0
74	16957.5
75	12756.0
76	9453.5
77	6819.5
78	4695.0
79	3195.5
80	2150.5
81	1396.0
82	858.5
83	517.5
84	304.0
85	164.5
86	89.5
87	47.5
88	21.0
89	12.5
90	5.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1339.0
92-93	3258.0
94-95	4764.0
96-97	6482.0
98-99	7956.0
100-101	9471.0
102-103	10832.0
104-105	11871.0
106-107	13140.0
108-109	14452.0
110-111	15075.0
112-113	16369.0
114-115	17164.0
116-117	17459.0
118-119	18582.0
120-121	19236.0
122-123	20084.0
124-125	20661.0
126-127	21810.0
128-129	22576.0
130-131	23911.0
132-133	26682.0
134-135	105106.0
136-137	108549.0
138-139	103749.0
140-141	106164.0
142-143	111573.0
144-145	110249.0
146-147	115036.0
148-149	86213.0
150-151	86231.0
152-153	83721.0
154-155	93425.0
156-157	84688.0
158-159	90334.0
160-161	90705.0
162-163	90942.0
164-165	94781.0
166-167	102822.0
168-169	99454.0
170-171	102315.0
172-173	110295.0
174-175	112735.0
176-177	338012.0
178-179	4634295.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.23413039989963
#Duplication Level	Percentage of total
1	87.25076774517181
2	10.679253177858962
3	1.6252622602422404
4	0.32114596068978046
5	0.07492927151311533
6	0.02583441569391256
7	0.008322845860633851
8	0.0020947313677601074
9	0.0031632194190855086
>10	0.009226372182700204
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.354175355958534E-5	1.354175355958534E-5	1.354175355958534E-5	0.0	0.0	0.0
2	8.125052135751205E-5	1.354175355958534E-5	1.354175355958534E-5	0.0	1.354175355958534E-5	0.0
3	8.125052135751205E-5	1.354175355958534E-5	1.354175355958534E-5	0.0	1.354175355958534E-5	0.0
4	8.125052135751205E-5	2.708350711917068E-5	2.708350711917068E-5	0.0	2.708350711917068E-5	0.0
5	8.125052135751205E-5	2.708350711917068E-5	2.708350711917068E-5	0.0	2.708350711917068E-5	0.0
6	8.125052135751205E-5	2.708350711917068E-5	2.708350711917068E-5	0.0	2.708350711917068E-5	0.0
7	1.0833402847668272E-4	2.708350711917068E-5	4.0625260678756025E-5	0.0	2.708350711917068E-5	0.0
8	1.2187578203626807E-4	2.708350711917068E-5	5.416701423834136E-5	1.354175355958534E-5	4.0625260678756025E-5	0.0
9	1.2187578203626807E-4	2.708350711917068E-5	5.416701423834136E-5	1.354175355958534E-5	4.0625260678756025E-5	0.0
10-14	1.354175355958534E-4	3.791690996683896E-5	7.041711850984377E-5	2.1666805695336544E-5	5.416701423834136E-5	0.0
15-19	1.7875114698652652E-4	5.416701423834136E-5	1.1916743132435098E-4	5.416701423834136E-5	5.416701423834136E-5	0.0
20-24	2.3833486264870197E-4	6.77087677979267E-5	1.5166763986735582E-4	6.229206637409256E-5	5.416701423834136E-5	0.0
25-29	2.735434219036239E-4	8.125052135751205E-5	1.950012512580289E-4	1.0020897634093152E-4	7.312546922176085E-5	0.0
30-34	3.602106446849701E-4	8.125052135751205E-5	2.1666805695336544E-4	1.2458413274818512E-4	8.937557349326326E-5	0.0
35-39	4.7937807600932105E-4	9.479227491709738E-5	2.3562651193678497E-4	1.354175355958534E-4	1.1104237918859979E-4	1.354175355958534E-5
40-44	5.850037537740866E-4	9.479227491709738E-5	2.762517726155409E-4	1.354175355958534E-4	1.7333444556269238E-4	3.791690996683896E-5
45-49	7.177129386580232E-4	9.479227491709738E-5	3.4666889112538475E-4	1.4083423701968751E-4	2.78960123327458E-4	5.1458663526424297E-5
50-54	8.179219149989545E-4	1.0020897634093152E-4	4.035442560756431E-4	1.6520939342694118E-4	3.0333527973471164E-4	5.687536495025843E-5
55-59	8.74797279949213E-4	1.0833402847668272E-4	4.170860096352285E-4	2.112513555295313E-4	3.7916909966838954E-4	8.125052135751205E-5
60-64	9.91256360561647E-4	1.2187578203626805E-4	4.6041962102590165E-4	2.6271001905595564E-4	4.5771127031398454E-4	8.666722278134619E-5
65-69	0.0010616734790714906	1.3270918488393632E-4	5.254200381119113E-4	2.708350711917068E-4	4.685446731616528E-4	9.479227491709738E-5
70-74	0.001308133393855944	1.354175355958534E-4	5.606285973668331E-4	2.8437682475129214E-4	5.416701423834136E-4	9.479227491709738E-5
75-79	0.00650275005931288	1.4083423701968754E-4	5.822954030621696E-4	2.925018768870433E-4	5.687536495025843E-4	1.0291732705284859E-4
80-84	0.008704639188101456	1.706260948507753E-4	5.877121044860037E-4	3.412521897015506E-4	6.608375737077646E-4	1.1916743132435098E-4
85-89	0.008747972799492128	2.2208475837719958E-4	6.418791187243453E-4	3.656273461088042E-4	7.800050050321156E-4	1.2187578203626805E-4
90-94	0.008804848164442389	2.3020981051295083E-4	6.852127301150183E-4	3.7104404753263837E-4	7.854217064559496E-4	1.2187578203626805E-4
95-99	0.008831931671561561	2.5458496692020436E-4	7.041711850984377E-4	4.008359053637261E-4	7.98963460015535E-4	1.2187578203626805E-4
100-104	0.008869848581528398	2.708350711917068E-4	7.339630429295254E-4	4.2791941248289676E-4	8.125052135751204E-4	1.2187578203626805E-4
105-109	0.008956515804309746	2.8437682475129214E-4	7.854217064559496E-4	4.4146116604248215E-4	8.125052135751204E-4	1.2187578203626805E-4
110-114	0.008991724363564666	3.114603318704628E-4	8.504221235419593E-4	4.685446731616528E-4	8.260469671347057E-4	1.2187578203626805E-4
115-119	0.009054016429938758	3.195853840062141E-4	8.585471756777105E-4	4.847947774331552E-4	8.260469671347057E-4	1.2187578203626805E-4
120-124	0.009121725197736687	3.5208559254921887E-4	8.72088929237296E-4	4.956281802808235E-4	8.34172019270457E-4	1.2187578203626805E-4
125-129	0.009154225406279689	3.927108532279748E-4	8.937557349326323E-4	5.037532324165747E-4	8.74797279949213E-4	1.3812588630777046E-4
130-134	0.00922735087550145	4.062526067875602E-4	9.208392420518033E-4	5.281283888238283E-4	9.343809956113885E-4	1.4895928915543874E-4
135-139	0.009376310164656891	4.062526067875602E-4	9.425060477471399E-4	5.470868438072477E-4	9.668812041543934E-4	1.5979269200310704E-4
140-144	0.009435893880319065	4.062526067875602E-4	9.777146070020616E-4	5.660452987906673E-4	9.99381412697398E-4	1.706260948507753E-4
145-149	0.009484644193133574	4.062526067875602E-4	9.96673061985481E-4	5.931288059098378E-4	0.0010129231662569832	1.7604279627460943E-4
150-154	0.009549644610219583	4.1166930821139437E-4	0.0010183398676808174	6.229206637409256E-4	0.001037298322664237	1.7604279627460943E-4
155-159	0.009620061728729427	4.3062776319481377E-4	0.0010372983226642372	6.256290144528427E-4	0.001113132142597915	1.7604279627460943E-4
160-164	0.009728395757206108	4.3875281533056504E-4	0.0010643818297834077	6.472958201481794E-4	0.0011672991568362563	1.7604279627460943E-4
165-167	0.009840340919965348	4.604196210259016E-4	0.0010788263669136322	6.500041708600964E-4	0.0012232717382158755	1.8055671412780454E-4
>>END_MODULE
