##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479452
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7569931
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.233498165306926	39.0	39.0	39.0	37.0	39.0
2	39.9755843217065	41.0	40.0	41.0	38.0	41.0
3	40.02187998807387	41.0	40.0	41.0	38.0	41.0
4	40.04671139010382	41.0	40.0	41.0	38.0	41.0
5	40.05242769055623	41.0	40.0	41.0	38.0	41.0
6	40.04923756900823	41.0	40.0	41.0	38.0	41.0
7	40.07140923741577	41.0	40.0	41.0	38.0	41.0
8	40.039490320321285	41.0	40.0	41.0	38.0	41.0
9	40.00755621154275	41.0	40.0	41.0	38.0	41.0
10-14	39.947306309661215	41.0	40.0	41.0	38.0	41.0
15-19	39.80203164335315	41.0	40.0	41.0	37.8	41.0
20-24	39.596945309012725	40.4	39.0	41.0	36.8	41.0
25-29	39.4357746986069	40.0	39.0	41.0	36.0	41.0
30-34	39.20958616399541	40.0	38.4	41.0	35.0	41.0
35-39	38.75734196256214	40.0	37.4	41.0	35.0	41.0
40-44	38.14948957923131	39.2	35.6	41.0	34.4	41.0
45-49	37.648095603513426	38.6	35.0	40.8	34.0	41.0
50-54	37.054253044050206	36.8	35.0	39.8	34.0	41.0
55-59	36.40088087989177	35.6	35.0	38.8	33.6	41.0
60-64	35.708142650177386	35.0	34.8	37.0	32.8	39.8
65-69	35.390759123167705	35.0	34.8	36.0	33.0	39.0
70-74	35.20512139410518	35.0	35.0	36.0	33.0	38.2
75-79	35.053858615091734	35.0	35.0	35.0	33.0	37.0
80-84	34.99841847964004	35.0	35.0	35.0	33.0	37.4
85-89	34.79055544892021	35.0	34.6	35.0	32.4	38.2
90-94	39.413908497727576	40.6	39.8	40.6	36.8	40.6
95-99	39.645590264148936	41.0	39.6	41.0	36.8	41.0
100-104	39.50932265787373	41.0	39.0	41.0	36.0	41.0
105-109	39.336862428976005	40.4	39.0	41.0	35.4	41.0
110-114	39.108592400333954	40.0	38.0	41.0	35.0	41.0
115-119	38.81833299735154	40.0	38.0	41.0	35.0	41.0
120-124	38.68355850900431	40.0	37.4	41.0	34.8	41.0
125-129	38.103550115649725	39.6	35.8	40.6	33.8	41.0
130-134	37.755275129690865	38.8	35.0	41.0	34.0	41.0
135-139	37.20699124249036	37.4	35.0	40.2	33.0	41.0
140-144	36.57701117442281	36.2	35.0	39.4	33.0	41.0
145-149	35.9708644837815	35.0	35.0	38.0	33.0	40.4
150-154	35.60272261249375	35.0	35.0	36.8	33.0	39.2
155-159	35.130882561360735	35.0	35.0	36.0	33.0	37.6
160-164	34.78834089413663	35.0	35.0	35.0	33.0	36.4
165-169	34.567865690306796	35.0	34.8	35.0	33.0	36.0
170-174	34.44300702504694	35.0	34.0	35.0	33.0	35.8
175-178	34.08209846946376	34.75	33.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	6.0
29	60.0
30	520.0
31	3932.0
32	20011.0
33	77807.0
34	232852.0
35	600164.0
36	1455692.0
37	2402441.0
38	2207407.0
39	560915.0
40	8124.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.151655015085343	26.848791620425605	28.17274027993122	22.826813084557838
2	23.671153673659642	26.976599918810358	27.364159594057064	21.988086813472936
3	21.260920343923875	26.287069195214592	29.015508859988287	23.436501600873243
4	22.03061031864095	25.23449685340593	29.40291001331452	23.331982814638604
5	23.19708066031249	25.974794222034518	27.252097806439718	23.576027311213274
6	22.942732238906803	26.54699230415706	27.354991742989466	23.155283713946666
7	22.885664347535002	26.52238177600298	27.069625865810405	23.522328010651616
8	23.265562129958646	26.65377002775851	26.589225185804207	23.49144265647864
9	23.196288050710105	26.943521678070777	26.836743954469334	23.023446316749784
10-14	23.261340162809937	26.62184107094239	26.627103998702236	23.489714767545436
15-19	23.29641577974753	26.567304246234215	26.662338137560305	23.473941836457954
20-24	23.34378212958612	26.415065077871912	26.653326166381174	23.587826626160794
25-29	23.4464858398313	26.362707929570295	26.431614766369734	23.75919146422867
30-34	23.415135488024923	26.443659790293992	26.5134860542322	23.627718667448885
35-39	23.438100030238058	26.437186811874508	26.424597529356607	23.700115628530828
40-44	23.483590537351002	26.369165055797733	26.34365095269693	23.80359345415434
45-49	23.691949107594244	26.306723271321758	26.291053379482587	23.710274241601407
50-54	23.703402316348722	26.367949721074073	26.248905042859704	23.679742919717498
55-59	23.63428147495664	26.267127137618562	26.27538084561141	23.823210541813395
60-64	23.594592870133162	26.373101683489587	26.330837097458353	23.7014683489189
65-69	23.598093562543703	26.347035395699116	26.30042730904681	23.754443732710378
70-74	23.547992709576874	26.320176498306257	26.30739434745178	23.824436444665086
75-79	23.501934165582224	26.34559548825478	26.382018541516427	23.770451804646566
80-84	23.537160378344268	26.384797959188795	26.292775984351774	23.785265678115163
85-89	23.49784694206592	26.31034549720466	26.263454713127505	23.928352847601914
90-94	22.50675309357952	26.36526655014995	27.96730925277216	23.160671103498366
95-99	22.99996993494621	26.692503734047925	26.880971393418907	23.42655493758696
100-104	23.24693241503949	26.65689503840214	26.5815987578489	23.51457378870947
105-109	23.22845198898056	26.554332623768218	26.601889207122937	23.61532618012829
110-114	23.274574384799408	26.43127116521633	26.630999767386292	23.66315468259797
115-119	23.3947703682495	26.492749375310165	26.399507745378394	23.712972511061942
120-124	23.382951145617206	26.449504816154203	26.402535728990777	23.76500830923781
125-129	23.362719802938916	26.49840454714508	26.45482083207652	23.684054817839485
130-134	23.504188698594476	26.502353196663464	26.287267572434942	23.706190532307122
135-139	23.591349349009867	26.431914972624853	26.224141438942837	23.752594239422443
140-144	23.518990431357413	26.54784352075344	26.32988307715435	23.6032829707348
145-149	23.491463567606193	26.623080129400307	26.30360909804396	23.58184720494954
150-154	23.3849726912915	26.66944649805685	26.41187088489614	23.533709925755513
155-159	23.307251877094796	26.734564175355235	26.521113252928558	23.437070694621415
160-164	23.299442623715823	26.84492549054813	26.497009214611865	23.35862267112418
165-169	23.180574429663107	26.83883486650622	26.570182846400357	23.41040785743031
170-174	23.110041484262812	26.99425859177691	26.662842573412	23.23285735054828
175-178	23.111500429420424	27.04242966829895	26.54946234345161	23.296607558829013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	3.0
10	6.0
11	13.5
12	27.0
13	58.0
14	107.5
15	202.5
16	393.0
17	732.0
18	1354.0
19	2306.0
20	3767.5
21	6086.5
22	9223.5
23	13284.0
24	18641.0
25	25194.0
26	32918.5
27	42080.5
28	52751.0
29	64170.0
30	76414.5
31	89640.0
32	103706.5
33	118967.0
34	134946.5
35	151606.0
36	168916.0
37	187406.5
38	206725.0
39	225503.0
40	243588.0
41	260529.0
42	274189.5
43	285063.5
44	291819.5
45	293414.0
46	291472.0
47	286311.0
48	277944.5
49	265557.0
50	251656.5
51	238176.5
52	224043.0
53	210876.0
54	199734.5
55	189329.0
56	179893.5
57	171507.0
58	163370.0
59	154935.0
60	146168.0
61	136619.0
62	126717.0
63	115561.0
64	103392.0
65	90959.0
66	78094.0
67	65617.0
68	53932.0
69	43290.5
70	33746.5
71	25674.5
72	19007.0
73	13714.5
74	9660.5
75	6707.5
76	4450.5
77	2810.0
78	1796.5
79	1069.5
80	636.5
81	358.5
82	184.5
83	99.5
84	57.0
85	31.0
86	15.5
87	5.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1531.0
92-93	3103.0
94-95	4976.0
96-97	6363.0
98-99	8155.0
100-101	9617.0
102-103	11118.0
104-105	12307.0
106-107	13244.0
108-109	14716.0
110-111	15566.0
112-113	16262.0
114-115	17303.0
116-117	17719.0
118-119	18920.0
120-121	19923.0
122-123	20830.0
124-125	22515.0
126-127	22241.0
128-129	23004.0
130-131	24634.0
132-133	26829.0
134-135	104915.0
136-137	103364.0
138-139	102936.0
140-141	106248.0
142-143	103355.0
144-145	108430.0
146-147	112298.0
148-149	89515.0
150-151	85814.0
152-153	115671.0
154-155	86623.0
156-157	95352.0
158-159	88699.0
160-161	89821.0
162-163	90335.0
164-165	90879.0
166-167	100993.0
168-169	104674.0
170-171	104953.0
172-173	109639.0
174-175	109710.0
176-177	327289.0
178-179	4807542.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.74439497327377
#Duplication Level	Percentage of total
1	93.67239566399086
2	5.841917712141056
3	0.4021082838239472
4	0.04792013914705437
5	0.009236852556541188
6	0.005165428875967757
7	0.007279187274514586
8	0.0031401339224525022
9	0.0021071360015863182
>10	0.008729462266018144
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3210160039767866E-5	2.6420320079535733E-5	1.3210160039767866E-5	0.0	0.0	0.0
2	2.6420320079535733E-5	2.6420320079535733E-5	2.6420320079535733E-5	0.0	0.0	0.0
3	3.96304801193036E-5	6.605080019883933E-5	2.6420320079535733E-5	1.3210160039767866E-5	0.0	0.0
4	3.96304801193036E-5	6.605080019883933E-5	2.6420320079535733E-5	1.3210160039767866E-5	0.0	0.0
5	3.96304801193036E-5	6.605080019883933E-5	5.2840640159071466E-5	1.3210160039767866E-5	0.0	0.0
6	3.96304801193036E-5	6.605080019883933E-5	5.2840640159071466E-5	2.6420320079535733E-5	0.0	0.0
7	3.96304801193036E-5	1.0568128031814293E-4	5.2840640159071466E-5	2.6420320079535733E-5	0.0	0.0
8	3.96304801193036E-5	1.1889144035791079E-4	5.2840640159071466E-5	3.96304801193036E-5	0.0	0.0
9	5.2840640159071466E-5	1.1889144035791079E-4	5.2840640159071466E-5	3.96304801193036E-5	0.0	0.0
10-14	5.284064015907146E-5	1.2945956838972508E-4	6.340876819088575E-5	5.284064015907146E-5	0.0	0.0
15-19	5.548267216702504E-5	1.426697284294929E-4	1.083233123260965E-4	7.926096023860719E-5	1.585219204772144E-5	0.0
20-24	6.605080019883933E-5	1.79658176540843E-4	1.1889144035791079E-4	9.247112027837506E-5	2.9062352087489308E-5	0.0
25-29	8.982908827042147E-5	2.0079443260447158E-4	1.3210160039767865E-4	1.1096534433405008E-4	6.605080019883934E-5	0.0
30-34	1.1624940834995723E-4	2.4835100874763587E-4	1.585219204772144E-4	1.4795379244540008E-4	1.1360737634200365E-4	0.0
35-39	1.7437411252493583E-4	2.8533945685898593E-4	2.1664662465219298E-4	2.2193068866810015E-4	1.7173208051698223E-4	1.3210160039767865E-5
40-44	2.16646624652193E-4	3.196858729623823E-4	2.5627710477149656E-4	2.3778288071582158E-4	2.087205286283323E-4	1.3210160039767865E-5
45-49	2.6156116878740376E-4	3.3025400099419663E-4	2.879814888669395E-4	2.5099304075558947E-4	2.747713288271716E-4	2.3778288071582158E-5
50-54	2.9590758489080016E-4	3.672424491055467E-4	3.170438409544288E-4	2.826974248510323E-4	3.249699369782895E-4	2.642032007953573E-5
55-59	3.4082212902601097E-4	4.200830892646181E-4	3.223279049703359E-4	2.90623520874893E-4	4.0158886520894315E-4	4.7556576143164315E-5
60-64	3.9366276918508246E-4	4.385773133202931E-4	3.698844811135003E-4	3.0911774493056805E-4	4.5971356938392173E-4	6.340876819088575E-5
65-69	4.412193453282467E-4	4.5971356938392173E-4	4.0423089721689664E-4	3.540322890657788E-4	5.125542095429931E-4	6.605080019883933E-5
70-74	7.212747381713255E-4	4.88775921471411E-4	4.3329324930438596E-4	3.883787051691753E-4	5.574687536782039E-4	6.605080019883933E-5
75-79	0.004834918574555039	5.178382735589003E-4	4.465034093441538E-4	4.1479902524871093E-4	5.997412658054611E-4	6.86928322067929E-5
80-84	0.006546955315708954	5.706789137179718E-4	4.7556576143164316E-4	4.8349185745550385E-4	7.23916770179279E-4	7.92609602386072E-5
85-89	0.006586585795828258	5.94457201789554E-4	4.8349185745550385E-4	5.442585936384361E-4	0.0010541707711734759	7.92609602386072E-5
90-94	0.006620932211931654	6.076673618293218E-4	4.914179534793647E-4	5.838890737577396E-4	0.0010753070272371042	7.92609602386072E-5
95-99	0.0066526365960270974	6.288036178929504E-4	5.099121775350396E-4	6.023832978134147E-4	0.001083233123260965	7.92609602386072E-5
100-104	0.0066711308200827725	6.446558099406719E-4	5.38974529622529E-4	6.235195538770433E-4	0.0010885171872768722	7.92609602386072E-5
105-109	0.0066896250441384475	6.552239379724861E-4	5.680368817100182E-4	6.578659699804397E-4	0.0010964432833007328	7.92609602386072E-5
110-114	0.00671340333221003	6.605080019883933E-4	6.235195538770433E-4	6.922123860838362E-4	0.0011096534433405008	7.92609602386072E-5
115-119	0.006753033812329333	6.895703540758826E-4	6.842862900599755E-4	7.265588021872326E-4	0.0011122954753484543	7.92609602386072E-5
120-124	0.006845504932607709	7.186327061633718E-4	7.133486421474648E-4	7.899675703781185E-4	0.0011492839234598044	7.92609602386072E-5
125-129	0.0069353340208781296	7.450530262429077E-4	7.292008341951862E-4	7.926096023860719E-4	0.001188914403579108	7.92609602386072E-5
130-134	0.007030447173164459	7.74115378330397E-4	7.397689622270005E-4	8.031777304178863E-4	0.00121269269165069	8.190299224656077E-5
135-139	0.007159906741554184	7.97893666401979E-4	7.529791222667683E-4	8.375241465212827E-4	0.0012179767556665972	9.247112027837506E-5
140-144	0.00723916770179279	8.058197624258398E-4	7.820414743542577E-4	8.692285306167254E-4	0.001244397075746133	9.247112027837506E-5
145-149	0.007286724277935955	8.216719544735611E-4	8.005356984099326E-4	8.7979665864854E-4	0.0012945956838972508	9.247112027837506E-5
150-154	0.0073157866300234435	8.375241465212827E-4	8.29598050497422E-4	8.850807226644471E-4	0.0013500783560642757	9.247112027837506E-5
155-159	0.007402973686285913	8.560183705769577E-4	8.428082105371899E-4	9.009329147121684E-4	0.001405561028231301	9.247112027837506E-5
160-164	0.007532433254675637	8.613024345928649E-4	8.454502425451433E-4	9.934040349905435E-4	0.0014636857324062794	9.247112027837506E-5
165-167	0.0076442792763456715	8.894841093443697E-4	8.454502425451435E-4	0.0010303924831018935	0.001479537924454001	1.0568128031814292E-4
>>END_MODULE
