##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479477
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13844367
Total Bases	2.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.38042288246187	39.0	39.0	39.0	37.0	39.0
2	40.098710399688194	41.0	40.0	41.0	38.0	41.0
3	40.112713929065876	41.0	40.0	41.0	38.0	41.0
4	40.11497882135023	41.0	40.0	41.0	38.0	41.0
5	40.099630412860336	41.0	40.0	41.0	38.0	41.0
6	40.08171460638106	41.0	40.0	41.0	38.0	41.0
7	40.10504792310114	41.0	40.0	41.0	38.0	41.0
8	40.12031745474531	41.0	40.0	41.0	38.0	41.0
9	40.11487740826287	41.0	40.0	41.0	38.0	41.0
10-14	40.08754792472636	41.0	40.0	41.0	38.0	41.0
15-19	39.990679458295205	41.0	40.0	41.0	38.0	41.0
20-24	39.85681250720961	41.0	40.0	41.0	38.0	41.0
25-29	39.67248919361932	40.2	39.4	41.0	37.4	41.0
30-34	39.461121191023025	40.0	39.0	41.0	36.6	41.0
35-39	39.148692981051425	40.0	38.2	41.0	35.6	41.0
40-44	38.75895338515657	40.0	37.8	41.0	35.0	41.0
45-49	38.33024315232325	39.4	36.6	40.8	34.8	41.0
50-54	37.622745193044935	38.4	35.4	40.0	34.0	41.0
55-59	36.790968211114304	36.8	35.0	39.4	33.4	40.8
60-64	35.73485266606989	35.4	34.6	38.0	32.4	39.8
65-69	35.34613386079696	35.0	34.0	37.0	32.8	39.0
70-74	35.19233985923661	35.0	34.0	36.0	33.0	38.8
75-79	34.929906900041004	35.0	34.0	35.6	32.8	38.0
80-84	34.809736277577734	35.0	34.0	35.0	32.0	38.4
85-89	35.29892772995688	35.6	34.4	35.8	32.0	39.4
90-94	39.957351687793754	41.0	40.0	41.0	38.0	41.0
95-99	39.87652908499261	41.0	40.0	41.0	38.0	41.0
100-104	39.77536237960803	41.0	40.0	41.0	37.4	41.0
105-109	39.736707009486615	41.0	40.0	41.0	37.0	41.0
110-114	39.599569204827496	41.0	39.2	41.0	36.6	41.0
115-119	39.40454050427071	40.2	39.0	41.0	35.8	41.0
120-124	39.16402014372507	40.0	38.2	41.0	35.0	41.0
125-129	38.423024494833115	39.6	37.4	40.4	34.6	40.8
130-134	38.41601336916801	39.8	36.6	41.0	34.6	41.0
135-139	37.87644736449884	38.8	35.4	40.6	34.0	41.0
140-144	37.17510614569151	37.4	35.0	39.8	34.0	41.0
145-149	36.513753194167556	36.2	35.0	39.0	33.6	40.8
150-154	35.92875052858975	35.2	35.0	37.2	33.4	39.4
155-159	35.32041212774348	35.0	35.0	36.2	33.0	38.2
160-164	34.846525656115816	35.0	34.6	35.4	33.0	36.8
165-169	34.53552995815005	35.0	34.0	35.0	33.0	36.0
170-174	34.34870773564279	35.0	34.0	35.0	32.8	35.8
175-176	33.419555031963064	34.5	32.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	1.0
30	128.0
31	1638.0
32	13739.0
33	68430.0
34	240886.0
35	662983.0
36	1745231.0
37	4514470.0
38	5363324.0
39	1224857.0
40	8680.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.675094787649012	28.737493017918407	29.931473212173586	20.655938982258995
2	21.19177424291049	29.315850988347826	29.094540761596395	20.39783400714529
3	19.515807403834355	28.405191801113045	30.77334630034006	21.30565449471254
4	20.263974510355006	27.717027437946424	30.794185100698357	21.224812951000217
5	20.916117002676973	28.585835668759717	29.432172666327034	21.065874662236272
6	20.77482488003966	28.806134653899306	29.52456403387746	20.894476432183573
7	20.724826205488487	28.62918181813585	29.34398517462012	21.302006801755542
8	20.971287455757277	28.9069482194455	28.96476234702533	21.1570019777719
9	21.00924513197317	28.95297415909301	29.066688278344543	20.971092430589277
10-14	21.10081161529451	28.796786447513274	28.828056927413147	21.274345009779065
15-19	21.19029638552633	28.720137222597465	28.7937888384496	21.295777553426603
20-24	21.243893635584783	28.650552242655802	28.75928816391533	21.346265957844082
25-29	21.302269724574625	28.587602452318695	28.610478182209416	21.49964964089727
30-34	21.327310956145556	28.617759121814668	28.583215108354175	21.471714813685594
35-39	21.33641213065213	28.630684234244875	28.546486813012105	21.486416822090888
40-44	21.40373770790676	28.554871450605145	28.457286635062477	21.584104206425618
45-49	21.523864543608244	28.494796475707414	28.44043501591658	21.540903964767764
50-54	21.531357843951984	28.552987651945372	28.399251478958913	21.51640302514373
55-59	21.492183788540135	28.475718680384592	28.407403530981227	21.624694000094046
60-64	21.476347744898703	28.536102806289378	28.397951311172264	21.589598137639662
65-69	21.442784635801697	28.553746083154252	28.44433118538392	21.55913809566013
70-74	21.387989786748644	28.521566930434595	28.44917214344289	21.64127113937387
75-79	21.3518393437562	28.519066274391598	28.500827809606605	21.628266572245593
80-84	21.360486904168315	28.568700901962508	28.47675014682867	21.594062047040506
85-89	21.186044836863978	28.55634352946581	28.72979602462142	21.52781560904879
90-94	20.632031674288427	28.539172149910186	29.794699629835915	21.034096545965475
95-99	20.952852387079876	28.818797911266063	28.983533472431084	21.244816229222977
100-104	21.140570944840373	28.6981099263303	28.82341736514973	21.337901763679596
105-109	21.205610518081954	28.67126513554603	28.75528893242418	21.367835413947837
110-114	21.244444325221394	28.555339915719053	28.696979817822864	21.50323594123669
115-119	21.337845229129897	28.59103443712177	28.579342821705357	21.49177751204298
120-124	21.340203664686108	28.598925388635827	28.552162471534924	21.508708475143134
125-129	21.315027414378726	28.579581389332304	28.550124567358015	21.555266628930955
130-134	21.433984866683954	28.519494508138145	28.498303958067527	21.548216667110374
135-139	21.46646621727245	28.58283004378378	28.444057034234916	21.50664670470885
140-144	21.436048089910855	28.58318052651585	28.4362677423513	21.544503641221997
145-149	21.4093814006233	28.616736470325854	28.480943945976943	21.492938183073903
150-154	21.369747563575984	28.708539185657667	28.51365602169586	21.408057229070486
155-159	21.304838421391324	28.70521113566481	28.53610842681106	21.453842016132803
160-164	21.276362292197167	28.77065647213108	28.56649400141332	21.386487234258432
165-169	21.261771552664996	28.84147618434	28.568127466651987	21.328624796343014
170-174	21.176318887107517	28.8916334609431	28.608825156191468	21.323222495757914
175-176	21.26053808908008	28.940093066394248	28.61321662444481	21.186152220080867
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	3.0
9	6.5
10	22.0
11	54.0
12	116.0
13	224.5
14	401.0
15	677.0
16	1174.0
17	1897.0
18	3137.0
19	5237.5
20	8446.0
21	13380.5
22	20444.0
23	30084.0
24	42751.0
25	58303.0
26	77955.5
27	102157.5
28	130646.0
29	163065.0
30	198535.5
31	237478.5
32	278831.0
33	321392.5
34	365340.0
35	410776.0
36	456267.0
37	501401.5
38	544801.0
39	585164.0
40	620271.5
41	650525.5
42	673879.0
43	686231.0
44	687012.5
45	675072.0
46	651225.0
47	616204.0
48	571914.0
49	520744.0
50	465968.5
51	410760.5
52	355401.5
53	302529.5
54	255375.5
55	212756.5
56	174800.5
57	142805.5
58	116120.0
59	94176.5
60	76474.0
61	61790.5
62	50056.5
63	40960.0
64	33739.5
65	28032.0
66	23184.0
67	19006.5
68	15689.0
69	12850.0
70	10325.5
71	8103.5
72	6234.0
73	4650.5
74	3394.5
75	2441.5
76	1661.5
77	1112.0
78	751.0
79	474.0
80	291.5
81	170.0
82	96.5
83	64.5
84	36.0
85	14.0
86	5.0
87	4.0
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1537.0
92-93	3635.0
94-95	5753.0
96-97	7658.0
98-99	9656.0
100-101	11449.0
102-103	13576.0
104-105	15423.0
106-107	17252.0
108-109	19103.0
110-111	20516.0
112-113	22007.0
114-115	23090.0
116-117	24750.0
118-119	26526.0
120-121	28261.0
122-123	30272.0
124-125	31834.0
126-127	34245.0
128-129	36704.0
130-131	39693.0
132-133	93977.0
134-135	141311.0
136-137	149494.0
138-139	148764.0
140-141	146114.0
142-143	157549.0
144-145	161461.0
146-147	147166.0
148-149	153198.0
150-151	160864.0
152-153	149247.0
154-155	142616.0
156-157	149922.0
158-159	158480.0
160-161	172828.0
162-163	182441.0
164-165	194948.0
166-167	208933.0
168-169	225880.0
170-171	241805.0
172-173	268373.0
174-175	1176542.0
176-177	8689514.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.58678817967217
#Duplication Level	Percentage of total
1	82.2901917329158
2	11.622210410548032
3	2.4934037552386936
4	1.1356374353082792
5	0.7740156131017641
6	0.48906739828876417
7	0.35205303761553763
8	0.26217569000003876
9	0.16239441330495186
>10	0.3957708124308725
>50	0.002509772608627443
>100	0.0015282941444931066
>500	0.0
>1k	0.01904163449412441
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.223154370293709E-6	0.0	7.223154370293709E-6	7.223154370293709E-6	0.0	0.0
2	4.333892622176225E-5	7.223154370293709E-6	2.1669463110881124E-5	7.223154370293709E-6	0.0	0.0
3	4.333892622176225E-5	7.223154370293709E-6	2.8892617481174835E-5	7.223154370293709E-6	0.0	0.0
4	4.333892622176225E-5	7.223154370293709E-6	2.8892617481174835E-5	1.4446308740587417E-5	0.0	0.0
5	5.056208059205596E-5	1.4446308740587417E-5	3.6115771851468545E-5	2.8892617481174835E-5	7.223154370293709E-6	0.0
6	5.778523496234967E-5	2.1669463110881124E-5	4.333892622176225E-5	2.8892617481174835E-5	7.223154370293709E-6	0.0
7	6.500838933264338E-5	2.1669463110881124E-5	5.056208059205596E-5	3.6115771851468545E-5	7.223154370293709E-6	0.0
8	6.500838933264338E-5	2.1669463110881124E-5	5.778523496234967E-5	4.333892622176225E-5	7.223154370293709E-6	0.0
9	7.223154370293709E-5	2.1669463110881124E-5	6.500838933264338E-5	5.056208059205596E-5	1.4446308740587417E-5	0.0
10-14	7.367617457699583E-5	3.756040272552728E-5	8.234395982134828E-5	6.789765108076087E-5	2.311409398493987E-5	0.0
15-19	9.101174506570073E-5	5.3451342340173435E-5	1.1845973167281681E-4	1.0834731555440563E-4	3.6115771851468545E-5	0.0
20-24	1.0545805380628814E-4	8.378859069540702E-5	2.0658221499040007E-4	1.7191107401299025E-4	5.056208059205596E-5	0.0
25-29	1.256828860431105E-4	1.0834731555440563E-4	2.6436744995274975E-4	2.3114093984939865E-4	5.056208059205596E-5	0.0
30-34	1.6179865789457904E-4	1.213489934209343E-4	3.5248993327033297E-4	2.8170302044145464E-4	6.211912758452589E-5	0.0
35-39	2.383640942196924E-4	1.3290604041340425E-4	4.4783557095820996E-4	3.2793120841133436E-4	8.66778524435245E-5	4.333892622176225E-6
40-44	2.961493291820421E-4	1.6757718139081402E-4	4.969530206762071E-4	3.799379198774491E-4	1.1990436254687557E-4	2.744798660711609E-5
45-49	3.5248993327033297E-4	1.935805371238714E-4	5.504043630163806E-4	4.593926179506798E-4	1.5457550352428537E-4	4.0449664473644766E-5
50-54	4.001627521142715E-4	2.600335573305735E-4	6.110788597268478E-4	5.041761750465008E-4	1.8202349013140146E-4	6.067449671046715E-5
55-59	4.666157723209736E-4	2.744798660711609E-4	6.443053698301988E-4	5.951879201122016E-4	2.2247315460504624E-4	8.089932894728953E-5
60-64	4.998422824243245E-4	3.004832218042182E-4	6.731979873113737E-4	6.197466449712003E-4	2.874815439376896E-4	1.0112416118411192E-4
65-69	5.229563764092645E-4	3.394882554038043E-4	7.309832222737232E-4	6.7753187993355E-4	3.1348489967074697E-4	1.1701510079875808E-4
70-74	0.0018505721496692481	3.568238258925092E-4	7.786560411176619E-4	7.223154370293708E-4	3.351543627816281E-4	1.357953021615217E-4
75-79	0.010605035246465224	3.9149496686991897E-4	8.465536921984228E-4	7.873238263620142E-4	3.626023493887442E-4	1.4446308740587418E-4
80-84	0.01238915437592777	4.001627521142715E-4	8.768909405536561E-4	8.696677861833625E-4	6.067449671046715E-4	1.5168624177616788E-4
85-89	0.012435382563897649	4.189429534770351E-4	9.043389271607723E-4	9.216744976494771E-4	8.21994967339424E-4	1.5168624177616788E-4
90-94	0.012494612429734056	4.3627852396574E-4	9.563456386268869E-4	9.67902685619357E-4	8.234395982134829E-4	1.5168624177616788E-4
95-99	0.012511948000222765	4.536140944544449E-4	0.0010141308735892366	0.0010184647662114129	8.27773490835659E-4	1.5168624177616788E-4
100-104	0.01253795135595582	4.637265105728561E-4	0.0010444681219444704	0.001061803692433175	8.306627525837765E-4	1.5168624177616788E-4
105-109	0.012545174510326112	4.7383892669126726E-4	0.0011051426186549375	0.0011109211421511723	8.378859069540702E-4	1.5168624177616788E-4
110-114	0.01256251008081482	4.983976515502659E-4	0.001190375840224403	0.0011484815448766996	8.378859069540702E-4	1.5168624177616788E-4
115-119	0.012598625852666287	5.345134234017345E-4	0.001225046981201813	0.0011932651019725207	8.537768465687164E-4	1.5168624177616788E-4
120-124	0.012675191288991399	5.446258395201456E-4	0.0012886107396603975	0.0012221577194536956	8.812248331758324E-4	1.5168624177616788E-4
125-129	0.012822543638145393	5.821862422456728E-4	0.0013160587262675137	0.0012698305382976341	9.144513432791836E-4	1.5168624177616788E-4
130-134	0.01295111578593662	6.038557053565541E-4	0.0013723993303558046	0.001314614095393455	9.418993298862995E-4	1.5168624177616788E-4
135-139	0.013140362430438316	6.226359067193178E-4	0.0014142936257035082	0.0013565083907411586	9.563456386268871E-4	1.5168624177616788E-4
140-144	0.013315162766199424	6.601963094448449E-4	0.0014561879210512117	0.0014186275183256845	9.693473164934157E-4	1.5457550352428537E-4
145-149	0.013398951356894829	6.87644296051961E-4	0.0015009714781470326	0.001476412753288034	0.0010069077192189431	1.589093961464616E-4
150-154	0.013494296994582707	7.107583900369009E-4	0.0015833154379683808	0.0015096392633913852	0.001047357383692588	1.589093961464616E-4
155-159	0.013670541961217874	7.425402692661932E-4	0.0016468791964269658	0.0015602013439834411	0.0010603590615591164	1.589093961464616E-4
160-164	0.013828006726490278	7.526526853846045E-4	0.0016844395991524926	0.0015992063775830268	0.001069026846803469	1.589093961464616E-4
165	0.013911795317185683	7.584312088808394E-4	0.0017046644313893152	0.0016324328876863782	0.0010762500011737626	1.589093961464616E-4
>>END_MODULE
