##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479493
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8336557
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.19390498979375	39.0	39.0	39.0	37.0	39.0
2	39.9035156839928	41.0	40.0	41.0	38.0	41.0
3	39.95665512753047	41.0	40.0	41.0	38.0	41.0
4	39.97524109773375	41.0	40.0	41.0	38.0	41.0
5	39.97502554111967	41.0	40.0	41.0	38.0	41.0
6	39.98047503303822	41.0	40.0	41.0	38.0	41.0
7	39.993793600883436	41.0	40.0	41.0	38.0	41.0
8	39.98680450454546	41.0	40.0	41.0	38.0	41.0
9	39.9630775630755	41.0	40.0	41.0	38.0	41.0
10-14	39.9271674625388	41.0	40.0	41.0	38.0	41.0
15-19	39.81238501698003	40.8	40.0	41.0	38.0	41.0
20-24	39.664899118425026	40.0	39.8	41.0	37.0	41.0
25-29	39.42690856669007	40.0	39.0	41.0	36.2	41.0
30-34	39.08027525032217	40.0	38.0	41.0	35.0	41.0
35-39	38.69581540676804	40.0	37.4	41.0	35.0	41.0
40-44	38.28114069153489	39.6	36.2	41.0	34.4	41.0
45-49	37.87535583334943	38.8	35.0	41.0	34.0	41.0
50-54	37.253212303352576	37.2	35.0	40.0	34.0	41.0
55-59	36.59524947769205	36.0	35.0	39.2	33.6	41.0
60-64	35.81742837000935	35.0	34.6	37.4	32.6	40.0
65-69	35.4402716133291	35.0	34.8	36.4	33.0	39.0
70-74	35.21609755682112	35.0	35.0	36.0	33.0	38.0
75-79	35.008434249295	35.0	35.0	35.2	33.0	37.0
80-84	34.916864120283705	35.0	34.6	35.0	33.0	36.6
85-89	34.772931103331985	35.0	34.0	35.0	32.6	36.2
90-94	39.173177292118226	40.6	38.8	40.6	35.8	40.6
95-99	39.44876761806918	40.4	39.0	41.0	36.0	41.0
100-104	39.34787480381854	40.0	39.0	41.0	35.4	41.0
105-109	39.19933181853142	40.0	38.8	41.0	35.0	41.0
110-114	39.00388108396176	40.0	38.0	41.0	35.0	41.0
115-119	38.736753209865164	40.0	38.0	41.0	34.4	41.0
120-124	38.40254827960426	40.0	36.8	41.0	34.0	41.0
125-129	37.519264976660914	38.8	35.6	40.2	33.0	40.6
130-134	37.483866823385355	38.6	35.0	40.0	33.0	41.0
135-139	37.22820952286799	37.6	35.0	40.0	33.0	41.0
140-144	36.929458853385405	36.6	35.0	40.0	33.0	41.0
145-149	36.32145866660084	35.6	35.0	39.0	33.0	41.0
150-154	35.713623689816224	35.0	35.0	37.0	33.0	39.6
155-159	35.196719942977055	35.0	35.0	36.0	33.0	38.4
160-164	34.811799380094115	35.0	34.4	35.4	33.0	36.8
165-169	34.55488846491218	35.0	34.0	35.0	33.0	36.0
170-174	34.40375657252145	35.0	34.0	35.0	33.0	36.0
175-178	34.15763871902823	35.0	33.75	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	32.0
29	431.0
30	2954.0
31	13756.0
32	51730.0
33	138897.0
34	303679.0
35	668694.0
36	1531384.0
37	2504805.0
38	2372323.0
39	737115.0
40	10757.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.6983102256723	27.68514627801381	28.556657142750897	22.059886353562987
2	22.452206588403342	28.474776817335982	28.29958458869771	20.773432005562967
3	19.61219721762833	26.905519868693993	31.0012754665985	22.481007447079172
4	20.830649871403747	25.31324382475883	31.378841408989345	22.477264894848076
5	22.35839088007195	26.33845123352482	28.677390438282856	22.625767448120367
6	22.128883662643943	26.88179304717763	28.58113967192931	22.408183618249115
7	22.27561090267841	26.96862745615486	27.874732938310142	22.881028702856586
8	22.62843041797711	27.308264071126725	27.33802455857976	22.725280952316407
9	22.441926565127545	27.535252263014577	27.615369270551383	22.407451901306498
10-14	22.673489787210716	27.397113700536085	27.204938441613248	22.72445807063995
15-19	22.65876908176841	27.326341078217304	27.33517206203952	22.679717777974766
20-24	22.668787606202418	27.176211954167652	27.3807880159639	22.77421242366603
25-29	22.779819054796842	27.03722172114939	27.17123148081396	23.011727743239803
30-34	22.781155337869098	27.127601958458392	27.124159290220174	22.96708341345234
35-39	22.82911278600986	27.14946230200309	27.041818342992197	22.97960656899485
40-44	22.90975279123024	27.020778482052005	26.95483039341061	23.114638333307145
45-49	23.014676202657764	26.96440029139128	26.983844769489373	23.037078736461584
50-54	23.052533557918455	27.02583092756398	26.92412946975592	22.997506044761646
55-59	23.01666743237046	26.951188602201125	26.90839395688172	23.123750008546693
60-64	22.93332607214225	27.00633846802703	27.00434963738627	23.05598582244445
65-69	22.974261436705827	27.03595501116348	26.945776295897694	23.044007256232998
70-74	22.932944619703314	26.990504593203163	26.92904756723909	23.147503219854432
75-79	22.890848104319325	27.030593085370857	27.000086486543545	23.078472323766274
80-84	22.917451413095357	27.074925535805725	26.92852217048357	23.079100880615343
85-89	22.8801482434535	27.01658730336757	26.884693525156727	23.218570928022203
90-94	21.48607793851211	27.121528746933844	29.207527192786113	22.184866121767936
95-99	22.285381164635194	27.308546614778006	27.68794869531799	22.718123525268815
100-104	22.57899282073051	27.49566030777004	27.223781920196743	22.701564951302707
105-109	22.552806669073316	27.35093459903972	27.258322524890794	22.83793620699617
110-114	22.554681864520383	27.192560470370676	27.339233462103763	22.913524203005185
115-119	22.67436670502829	27.24130822373043	27.117471652996993	22.96685341824428
120-124	22.694835745862495	27.207973395904013	27.003932414641863	23.093258443591626
125-129	22.643419750555786	27.196948759458305	27.09545173352725	23.064179756458657
130-134	22.818277609962575	27.16878073365884	26.951869544551627	23.06107211182696
135-139	22.937333599687552	27.097195156143844	26.862437017272466	23.103034226896142
140-144	22.85577533482785	27.204953354161006	27.007922786275014	22.931348524736133
145-149	22.829109884196537	27.27303427062427	27.020373049104624	22.877482796074567
150-154	22.76388524285672	27.28824425933848	27.042879661468262	22.904990836336538
155-159	22.676768391887876	27.373232452831676	27.155816170450674	22.794182984829778
160-164	22.643609210881625	27.48331000425815	27.15511987584926	22.717960909010966
165-169	22.55482097933817	27.512084681343772	27.20137648026764	22.731717859050416
170-174	22.434557313281292	27.628837450497638	27.33329856886919	22.60330666735188
175-178	22.402447296613623	27.74246328490666	27.270691652735916	22.584397765743798
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.0
4	3.0
5	4.5
6	7.5
7	14.5
8	24.5
9	37.5
10	78.5
11	136.0
12	262.5
13	495.0
14	874.5
15	1540.0
16	2571.5
17	4091.5
18	6356.0
19	9554.0
20	13909.0
21	19632.0
22	26455.0
23	34452.5
24	43690.5
25	53870.0
26	65003.0
27	76815.5
28	88954.5
29	100784.5
30	112746.0
31	125610.5
32	139211.0
33	153293.5
34	168692.0
35	185336.5
36	202178.0
37	219743.5
38	238046.5
39	255772.0
40	272011.5
41	285848.5
42	297112.5
43	305161.0
44	309115.0
45	308547.5
46	303610.0
47	295334.5
48	284082.5
49	270369.0
50	255135.0
51	239770.5
52	224788.0
53	211220.5
54	199536.5
55	189040.0
56	179267.5
57	170711.5
58	163481.5
59	156043.0
60	148121.0
61	139584.5
62	129292.5
63	117901.0
64	105287.5
65	91608.0
66	78017.5
67	64641.0
68	52179.0
69	40718.5
70	30731.5
71	22582.5
72	16090.5
73	11125.0
74	7327.5
75	4737.5
76	3024.5
77	1785.0
78	1023.0
79	588.0
80	293.5
81	138.5
82	74.5
83	38.0
84	17.0
85	6.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1286.0
92-93	2934.0
94-95	4167.0
96-97	5200.0
98-99	6310.0
100-101	7234.0
102-103	8023.0
104-105	9321.0
106-107	9815.0
108-109	11276.0
110-111	12248.0
112-113	12022.0
114-115	12850.0
116-117	13706.0
118-119	14215.0
120-121	14719.0
122-123	15184.0
124-125	15670.0
126-127	16519.0
128-129	17509.0
130-131	18012.0
132-133	19749.0
134-135	114798.0
136-137	123098.0
138-139	90552.0
140-141	90879.0
142-143	87826.0
144-145	87160.0
146-147	87679.0
148-149	86500.0
150-151	88871.0
152-153	86238.0
154-155	83031.0
156-157	84961.0
158-159	82343.0
160-161	85307.0
162-163	84949.0
164-165	90037.0
166-167	87123.0
168-169	101268.0
170-171	92358.0
172-173	101357.0
174-175	107129.0
176-177	218267.0
178-179	5926857.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.96722665214796
#Duplication Level	Percentage of total
1	92.2685595009428
2	6.85908462261405
3	0.6768747302093014
4	0.1313198753839936
5	0.03789618931948597
6	0.01133532732512159
7	0.005183862169400583
8	0.0010430857227771587
9	0.0020988483264871246
>10	0.0010944968488452134
>50	0.0
>100	0.00550946113773252
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.1995359715047831E-5	2.3990719430095662E-5	0.0	0.0	0.0
2	0.0	1.1995359715047831E-5	2.3990719430095662E-5	4.7981438860191325E-5	0.0	0.0
3	0.0	3.5986079145143493E-5	3.5986079145143493E-5	4.7981438860191325E-5	0.0	0.0
4	0.0	5.9976798575239156E-5	3.5986079145143493E-5	5.9976798575239156E-5	0.0	0.0
5	0.0	5.9976798575239156E-5	4.7981438860191325E-5	5.9976798575239156E-5	0.0	0.0
6	0.0	5.9976798575239156E-5	4.7981438860191325E-5	7.197215829028699E-5	0.0	0.0
7	0.0	7.197215829028699E-5	5.9976798575239156E-5	1.0795823743543047E-4	0.0	0.0
8	2.3990719430095662E-5	7.197215829028699E-5	7.197215829028699E-5	1.1995359715047831E-4	0.0	0.0
9	3.5986079145143493E-5	8.396751800533481E-5	7.197215829028699E-5	1.1995359715047831E-4	0.0	0.0
10-14	3.5986079145143493E-5	1.0555916549242091E-4	8.876566189135395E-5	1.43944316580574E-4	4.798143886019133E-6	0.0
15-19	4.318329497417219E-5	1.3674710075154526E-4	1.2954988492251657E-4	1.6553596406766006E-4	1.919257554407653E-5	0.0
20-24	4.7981438860191325E-5	1.511415324096027E-4	2.0871925904183226E-4	1.9912297126979398E-4	3.1187935259124356E-5	0.0
25-29	6.957308634727741E-5	1.9672389932678442E-4	2.54301625959014E-4	2.3990719430095662E-4	7.437123023329655E-5	0.0
30-34	1.0795823743543049E-4	2.327099784719279E-4	2.734942015030906E-4	2.9508584899017666E-4	1.3674710075154526E-4	9.596287772038266E-6
35-39	1.343480288085357E-4	2.7109512956008093E-4	2.998839928761958E-4	3.598607914514349E-4	1.5354060435261225E-4	5.5178654689220025E-5
40-44	1.4874246046659313E-4	2.998839928761958E-4	3.6705800728046363E-4	3.9344779865356885E-4	1.9912297126979398E-4	8.396751800533482E-5
45-49	2.279118345859088E-4	3.142784245342532E-4	4.054431583686168E-4	4.174385180836644E-4	2.5190255401600443E-4	1.2475174103649745E-4
50-54	2.7109512956008093E-4	3.310719281353201E-4	4.3183294974172195E-4	4.7261717277288453E-4	2.9028770510415754E-4	1.6073782018164094E-4
55-59	3.0948028064823403E-4	3.7185615116648276E-4	4.438283094567697E-4	4.918097483169611E-4	3.4306728785036796E-4	1.7993039572571746E-4
60-64	3.598607914514349E-4	3.7425522310949235E-4	4.606218130578367E-4	5.062041799750184E-4	4.078422303116262E-4	1.9672389932678442E-4
65-69	3.934477986535688E-4	3.838515108815306E-4	4.942088202599706E-4	5.541856188352099E-4	4.438283094567697E-4	2.2311369069988963E-4
70-74	6.165614893534584E-4	3.9104872671055926E-4	5.445893310631715E-4	6.093642735244298E-4	5.038051080320089E-4	2.279118345859088E-4
75-79	0.004265549914671009	4.4622738139977927E-4	5.589837627212289E-4	6.52547568498602E-4	5.397911871771523E-4	2.3750812235794706E-4
80-84	0.005846538325114313	4.966078922029802E-4	5.733781943792863E-4	7.029280793018029E-4	6.069652015814202E-4	2.5190255401600443E-4
85-89	0.005906515123689552	5.493874749491907E-4	5.781763382653054E-4	7.533085901050037E-4	7.461113742759749E-4	2.5190255401600443E-4
90-94	0.005937703058948677	5.709791224362767E-4	6.093642735244298E-4	7.749002375920898E-4	7.557076620480133E-4	2.5190255401600443E-4
95-99	0.005961693778378772	5.997679857523916E-4	6.14162417410449E-4	8.372761081103386E-4	7.581067339910228E-4	2.5190255401600443E-4
100-104	0.005966491922264791	6.237587051824871E-4	6.309559210115159E-4	8.51670539768396E-4	7.79698381478109E-4	2.5190255401600443E-4
105-109	0.005985684497808868	6.477494246125828E-4	6.621438562706402E-4	8.708631153124726E-4	7.988909570221855E-4	2.5190255401600443E-4
110-114	0.0059904826416948866	7.005290073587933E-4	7.317169426179176E-4	9.116473383436352E-4	8.132853886802431E-4	2.5190255401600443E-4
115-119	0.006016872433067992	7.389141584469464E-4	7.988909570221855E-4	9.764222808048932E-4	8.276798203383003E-4	2.5909976984503315E-4
120-124	0.006069652015814202	7.677030217630612E-4	8.540696117114055E-4	0.0010316009354941135	8.492714678253865E-4	2.7829234538910964E-4
125-129	0.006105638094959346	8.060881728512144E-4	8.948538347425683E-4	0.0010579907268672187	8.804594030845106E-4	2.9748492093318625E-4
130-134	0.006124830670503422	8.204826045092716E-4	9.908167124629509E-4	0.0010867795901833336	8.996519786285872E-4	2.998839928761958E-4
135-139	0.006215995404337786	8.51670539768396E-4	0.0010244037196650846	0.001139559172929544	9.356380577737307E-4	3.310719281353201E-4
140-144	0.006295164778457101	8.996519786285872E-4	0.0010675870146392569	0.001173146180131678	9.932157844059603E-4	3.3587007202133925E-4
145-149	0.006338348073431274	9.62027849146836E-4	0.0010795823743543049	0.001194737827618764	0.0010316009354941133	3.430672878503679E-4
150-154	0.006383930440348455	9.908167124629507E-4	0.0011035730937844004	0.0012355220506499265	0.0010819814462973145	3.550626475654158E-4
155-159	0.0064487053828097135	0.0010100092880070274	0.0011371601009865344	0.0012475174103649745	0.0011083712376704195	3.598607914514349E-4
160-164	0.006527874756929029	0.0010411972232661519	0.001177944324017697	0.0012523155542509936	0.0011563526765306109	3.622598633944445E-4
165-167	0.006589450936799608	0.0010675870146392569	0.0012195282376965294	0.0012835034895101178	0.0011675483455979888	3.838515108815306E-4
>>END_MODULE
