##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479494
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8357945
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.20794836529793	39.0	39.0	39.0	37.0	39.0
2	39.92113767199952	41.0	40.0	41.0	38.0	41.0
3	39.95410785785262	41.0	40.0	41.0	38.0	41.0
4	39.98578765474049	41.0	40.0	41.0	38.0	41.0
5	39.99992964777825	41.0	40.0	41.0	38.0	41.0
6	39.9927331419386	41.0	40.0	41.0	38.0	41.0
7	40.013006905405575	41.0	40.0	41.0	38.0	41.0
8	39.99896732988791	41.0	40.0	41.0	38.0	41.0
9	39.97234918392021	41.0	40.0	41.0	38.0	41.0
10-14	39.93466996971146	41.0	40.0	41.0	38.0	41.0
15-19	39.82375248939782	40.8	40.0	41.0	38.0	41.0
20-24	39.67518285894439	40.0	39.8	41.0	37.2	41.0
25-29	39.44591793796202	40.0	39.0	41.0	36.4	41.0
30-34	39.10666121875652	40.0	38.2	41.0	35.2	41.0
35-39	38.70213948524428	40.0	37.4	41.0	35.0	41.0
40-44	38.28855837170501	39.6	36.2	41.0	34.4	41.0
45-49	37.86926348522274	38.8	35.0	40.8	34.0	41.0
50-54	37.24090153739944	37.2	35.0	40.0	34.0	41.0
55-59	36.57903793336759	36.0	35.0	39.2	33.6	41.0
60-64	35.79742238074071	35.0	34.6	37.4	32.6	39.8
65-69	35.412240377269775	35.0	34.8	36.4	33.0	39.0
70-74	35.19083236369706	35.0	35.0	36.0	33.0	37.8
75-79	34.99443762790973	35.0	35.0	35.2	33.0	37.0
80-84	34.89415350304411	35.0	34.2	35.0	33.0	36.4
85-89	34.742648390244256	35.0	34.0	35.0	32.6	36.0
90-94	39.248269137091015	40.6	39.4	40.6	35.8	40.6
95-99	39.527943050081	41.0	39.0	41.0	36.0	41.0
100-104	39.4289883343159	40.2	39.0	41.0	36.0	41.0
105-109	39.28421781001994	40.0	39.0	41.0	35.2	41.0
110-114	39.08953477507801	40.0	38.0	41.0	35.0	41.0
115-119	38.82978463409315	40.0	38.0	41.0	35.0	41.0
120-124	38.49492849132866	40.0	37.2	41.0	34.0	41.0
125-129	37.610945177767135	38.8	35.6	40.2	33.4	40.6
130-134	37.57261771112932	38.8	35.0	40.0	33.0	41.0
135-139	37.307267544027106	37.6	35.0	40.0	33.0	41.0
140-144	36.993679874897225	36.8	35.0	40.0	33.2	41.0
145-149	36.375759959942584	35.6	35.0	39.0	33.0	41.0
150-154	35.76731306637442	35.0	35.0	37.2	33.0	39.6
155-159	35.249788206861794	35.0	35.0	36.0	33.0	38.4
160-164	34.86101580646944	35.0	34.8	35.4	33.0	36.8
165-169	34.605469496139364	35.0	34.0	35.0	33.0	36.0
170-174	34.451978915168574	35.0	34.0	35.0	33.0	36.0
175-178	34.20708806139574	35.0	33.75	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	26.0
29	361.0
30	2299.0
31	11419.0
32	43294.0
33	122404.0
34	286055.0
35	656546.0
36	1537765.0
37	2532278.0
38	2405606.0
39	749263.0
40	10629.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.665421344600855	27.646508800907398	28.598429398614133	22.08964045587761
2	22.41698168628772	28.480074946652557	28.3045892261794	20.798354140880324
3	19.608695678184052	26.91818383585917	31.02863203813856	22.444488447818216
4	20.86334619335255	25.27425102701681	31.384365415182796	22.47803736444784
5	22.374602847948868	26.29298230605729	28.689396735680837	22.643018110313
6	22.121765577543286	26.87846115283123	28.574500071488867	22.425273198136622
7	22.295504457136296	26.939217714402286	27.83995348138807	22.925324347073353
8	22.64377188411745	27.26044500173189	27.38838314920713	22.707399964943537
9	22.451774927927858	27.50990823701281	27.627700349786938	22.410616485272396
10-14	22.678309081957348	27.37448738894549	27.211988114303214	22.735215414793945
15-19	22.670639732613697	27.301514905876985	27.328689049760435	22.69915631174888
20-24	22.691884189235513	27.138367146469616	27.37058451569136	22.799164148603516
25-29	22.79696025757528	27.017062208473497	27.172368327381914	23.01360920656932
30-34	22.797431665319646	27.09862771291268	27.103722266657655	23.000218355110018
35-39	22.852631837132215	27.105562431913587	27.04291306056692	22.998892670387278
40-44	22.91106964690483	26.968937938691866	26.970584276398085	23.149408138005214
45-49	23.024396547237387	26.934929578981436	26.983139994340714	23.057533879440463
50-54	23.06501418709982	26.9861933764819	26.9359920411058	23.012800395312485
55-59	23.022025150919276	26.939483329933374	26.910210584061034	23.128280935086316
60-64	22.961845286131936	26.965008743177897	27.002888868017198	23.07025710267297
65-69	23.005492378808427	27.012151910547388	26.93942350661556	23.04293220402862
70-74	22.961225516559395	26.973285897430525	26.918858642884107	23.14662994312597
75-79	22.908092838610447	26.999318612410107	26.978033475932182	23.114555073047264
80-84	22.926157087657312	27.0480674376297	26.93002885278618	23.095746621926803
85-89	22.904453187954697	26.9840158077135	26.874469741066733	23.237061263265073
90-94	21.470729289311965	27.109314870295815	29.213245852754937	22.206709987637282
95-99	22.30457280578539	27.31423086559269	27.667578257046827	22.713618071575087
100-104	22.588783114281753	27.497127781980375	27.204219394009165	22.709869709728707
105-109	22.57500379689752	27.32843552203809	27.24322522421188	22.853335456852506
110-114	22.57439611827384	27.179861312502457	27.32450096784123	22.921241601382476
115-119	22.69643466721696	27.22695647220637	27.1033249362453	22.973283924331376
120-124	22.709768081278995	27.192585362758393	26.98886429274639	23.108782263216227
125-129	22.642895460392342	27.19462617902888	27.068851855107116	23.093626505471658
130-134	22.83808820556685	27.16877117699241	26.927380856799775	23.06575976064096
135-139	22.957424189550046	27.102994706756906	26.837510885201034	23.102070218492017
140-144	22.87084850329332	27.17668452699807	26.994595819868533	22.957871149840077
145-149	22.851044677360928	27.289971262356293	26.981408232375404	22.877575827907375
150-154	22.77278952725202	27.2937109987536	27.011139546519498	22.92235992747488
155-159	22.703036728344415	27.34346309684039	27.140264418980436	22.813235755834754
160-164	22.65318340640826	27.483762412965866	27.125551648971204	22.73750253165467
165-169	22.57277274598891	27.50453332898904	27.175943060023293	22.74675086499876
170-174	22.45830310218068	27.612171514028503	27.32212981363006	22.607395570160758
175-178	22.429500389036246	27.736057631616646	27.243857344793	22.590584634554112
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	4.0
7	10.5
8	20.5
9	33.0
10	61.0
11	127.0
12	268.5
13	500.5
14	878.0
15	1483.0
16	2511.0
17	4032.5
18	6294.0
19	9604.5
20	14000.0
21	19411.0
22	26205.5
23	34707.0
24	44195.5
25	54338.0
26	65336.5
27	76763.5
28	88425.0
29	100731.5
30	113189.5
31	126360.5
32	139368.0
33	153164.0
34	168565.0
35	184548.0
36	202077.0
37	220135.5
38	238397.0
39	255894.0
40	272217.5
41	285982.5
42	296770.5
43	305768.0
44	309154.0
45	308146.0
46	304543.5
47	296162.5
48	284724.0
49	271186.5
50	255581.5
51	240141.5
52	225657.5
53	211891.0
54	199230.0
55	188750.0
56	180109.5
57	172112.0
58	164759.0
59	157281.0
60	148768.0
61	139848.0
62	130449.5
63	118993.0
64	106458.5
65	92906.0
66	78822.5
67	65283.0
68	52509.5
69	41159.5
70	31426.0
71	23169.0
72	16401.5
73	11313.0
74	7523.5
75	4846.5
76	3009.0
77	1776.0
78	1037.5
79	603.5
80	311.0
81	148.0
82	76.5
83	38.0
84	18.5
85	8.0
86	4.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1160.0
92-93	2633.0
94-95	3979.0
96-97	5036.0
98-99	6094.0
100-101	6889.0
102-103	7908.0
104-105	8780.0
106-107	9214.0
108-109	10320.0
110-111	11144.0
112-113	11449.0
114-115	12136.0
116-117	13100.0
118-119	13602.0
120-121	14124.0
122-123	14686.0
124-125	14974.0
126-127	15724.0
128-129	16636.0
130-131	17255.0
132-133	19035.0
134-135	107072.0
136-137	114092.0
138-139	89705.0
140-141	86043.0
142-143	85635.0
144-145	85066.0
146-147	81596.0
148-149	81639.0
150-151	84875.0
152-153	89220.0
154-155	85321.0
156-157	84287.0
158-159	89723.0
160-161	84700.0
162-163	89056.0
164-165	86043.0
166-167	88124.0
168-169	89369.0
170-171	91299.0
172-173	98216.0
174-175	100521.0
176-177	218811.0
178-179	6011654.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.59278533922851
#Duplication Level	Percentage of total
1	93.49684525935169
2	5.704581984276523
3	0.6039626116694077
4	0.12620505442939267
5	0.037885304901476576
6	0.011331056553691075
7	0.0031088680966803546
8	0.0010425071004915459
9	0.0010487492497296062
>10	0.0075575982644436775
>50	0.0
>100	0.006431006106475764
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.1964663562634117E-5	1.1964663562634117E-5	0.0	1.1964663562634117E-5	0.0	0.0
2	1.1964663562634117E-5	1.1964663562634117E-5	0.0	1.1964663562634117E-5	0.0	0.0
3	2.3929327125268233E-5	2.3929327125268233E-5	0.0	2.3929327125268233E-5	1.1964663562634117E-5	0.0
4	2.3929327125268233E-5	5.982331781317058E-5	0.0	3.589399068790235E-5	1.1964663562634117E-5	0.0
5	2.3929327125268233E-5	5.982331781317058E-5	0.0	4.7858654250536466E-5	1.1964663562634117E-5	0.0
6	2.3929327125268233E-5	7.17879813758047E-5	0.0	4.7858654250536466E-5	2.3929327125268233E-5	0.0
7	2.3929327125268233E-5	8.375264493843882E-5	1.1964663562634117E-5	4.7858654250536466E-5	2.3929327125268233E-5	0.0
8	2.3929327125268233E-5	8.375264493843882E-5	1.1964663562634117E-5	4.7858654250536466E-5	4.7858654250536466E-5	0.0
9	2.3929327125268233E-5	8.375264493843882E-5	1.1964663562634117E-5	7.17879813758047E-5	4.7858654250536466E-5	0.0
10-14	4.0679856112955994E-5	1.2682543376392165E-4	1.1964663562634117E-5	1.2443250105139482E-4	4.7858654250536466E-5	0.0
15-19	7.178798137580469E-5	1.7707702072698495E-4	1.9143461700214586E-5	1.9622048242719952E-4	4.7858654250536466E-5	0.0
20-24	1.0528903935118022E-4	2.4647206939026276E-4	8.375264493843882E-5	2.2014980955246773E-4	5.743038510064376E-5	0.0
25-29	1.4118303003908257E-4	2.680084638030042E-4	1.3879009732655577E-4	2.727943292280578E-4	6.700211595075106E-5	0.0
30-34	1.8904168428961904E-4	2.8475899279069197E-4	1.8904168428961904E-4	3.134741853410138E-4	8.853851036349247E-5	4.7858654250536464E-6
35-39	2.4647206939026276E-4	2.9433072364079923E-4	2.4886500210278965E-4	3.6611870501660393E-4	1.2682543376392165E-4	4.0679856112955994E-5
40-44	2.8475899279069197E-4	3.015095217783797E-4	2.7518726194058465E-4	3.9722683027945265E-4	2.0339928056477998E-4	8.135971222591199E-5
45-49	3.1826005076606746E-4	3.374035124662821E-4	3.3501057975375526E-4	4.2833495554230137E-4	2.6082966566542374E-4	9.571730850107293E-5
50-54	3.4697524331638935E-4	3.589399068790235E-4	3.541540414539698E-4	4.4029961910493546E-4	3.206529834785943E-4	1.0768197206370705E-4
55-59	3.7569043586671124E-4	4.0919149384208674E-4	4.0679856112955996E-4	4.474784172425159E-4	3.6851163772913076E-4	1.1486077020128752E-4
60-64	3.9244096485439894E-4	4.4987134995504277E-4	4.3312082096735497E-4	4.785865425053646E-4	4.1876322469219405E-4	1.2443250105139482E-4
65-69	4.0919149384208674E-4	4.73800677080311E-4	4.666218789427305E-4	5.001229369181061E-4	4.594430808051501E-4	1.842558188645654E-4
70-74	6.413059669571886E-4	5.025158696306328E-4	4.809794752178915E-4	5.216593313308474E-4	4.833724079304182E-4	2.2493567497752139E-4
75-79	0.004484355903275267	5.479815911686425E-4	4.953370714930525E-4	5.455886584561158E-4	5.503745238811693E-4	2.345074058276287E-4
80-84	0.006152230003906463	5.790897164314913E-4	5.336239948934816E-4	5.790897164314913E-4	6.293413033945545E-4	2.3929327125268236E-4
85-89	0.006207267456294579	6.030190435567594E-4	5.55160389306223E-4	6.101978416943399E-4	8.901709690599783E-4	2.464720693902628E-4
90-94	0.006238375581557429	6.436988996697155E-4	5.766967837189644E-4	6.245554379695009E-4	9.21279094322827E-4	2.512579348153164E-4
95-99	0.006267090774107749	7.226656791831005E-4	6.006261108442326E-4	6.413059669571886E-4	9.308508251729342E-4	2.512579348153164E-4
100-104	0.006281448370382911	7.848819297087979E-4	6.317342361070813E-4	6.987363520578324E-4	9.33243757885461E-4	2.512579348153164E-4
105-109	0.006281448370382911	8.399193820969149E-4	6.748070249325641E-4	7.322374100332079E-4	9.404225560230414E-4	2.512579348153164E-4
110-114	0.006281448370382911	8.614557765096564E-4	7.154868810455202E-4	7.657384680085836E-4	9.452084214480952E-4	2.512579348153164E-4
115-119	0.006291020101233019	8.734204400722905E-4	7.729172661461638E-4	8.088112568340663E-4	9.643518831483098E-4	2.512579348153164E-4
120-124	0.006331699957345974	9.117073634727196E-4	7.896677951338516E-4	8.686345746472368E-4	0.0010074246719737928	2.512579348153164E-4
125-129	0.006396309140584198	9.47601354160622E-4	8.159900549716468E-4	9.093144307601927E-4	0.0010289610663865342	2.608296656654237E-4
130-134	0.006480061785522638	9.667448158608366E-4	8.662416419347099E-4	9.499942868731488E-4	0.0010672479897869632	2.7518726194058465E-4
135-139	0.006559028565036022	0.001002638806548739	8.949568344850318E-4	9.834953448485243E-4	0.0011198925094625532	2.8475899279069197E-4
140-144	0.0065997084211489795	0.001028961066386534	9.21279094322827E-4	0.0010098176046863196	0.0011438218365878215	2.871519255032188E-4
145-149	0.006645174142686988	0.0010385327972366413	9.739236139984172E-4	0.0010385327972366413	0.0011605723655755094	2.871519255032188E-4
150-154	0.006683461066087418	0.0010528903935118023	9.930670756986316E-4	0.0010863914514871779	0.0012275744815262602	2.96723656353326E-4
155-159	0.0067361055857630085	0.0010863914514871776	0.0010074246719737928	0.0011438218365878217	0.0013089341937521724	2.991165890658529E-4
160-164	0.006805500634426286	0.0011342501057377142	0.0010169964028239	0.0011605723655755092	0.0013448281844400747	2.991165890658529E-4
165-167	0.006859740442576894	0.0011366430384502412	0.001040925729949168	0.0011605723655755092	0.0013639716461402893	3.070930314409423E-4
>>END_MODULE
