##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479495
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8311892
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.183137244805394	39.0	39.0	39.0	37.0	39.0
2	39.88928465384295	41.0	40.0	41.0	38.0	41.0
3	39.9244221411924	41.0	40.0	41.0	38.0	41.0
4	39.959521370104426	41.0	40.0	41.0	38.0	41.0
5	39.97106723715852	41.0	40.0	41.0	38.0	41.0
6	39.96026187539491	41.0	40.0	41.0	38.0	41.0
7	39.98762965158835	41.0	40.0	41.0	38.0	41.0
8	39.969430185089024	41.0	40.0	41.0	38.0	41.0
9	39.95004591012491	41.0	40.0	41.0	38.0	41.0
10-14	39.90910264474081	41.0	40.0	41.0	38.0	41.0
15-19	39.796128871741836	40.6	40.0	41.0	37.8	41.0
20-24	39.641973427951186	40.0	39.6	41.0	37.0	41.0
25-29	39.411056207178824	40.0	39.0	41.0	36.2	41.0
30-34	39.06935287417113	40.0	38.0	41.0	35.0	41.0
35-39	38.676211457030476	40.0	37.4	41.0	35.0	41.0
40-44	38.26178641397169	39.6	36.0	41.0	34.4	41.0
45-49	37.861107074057266	38.8	35.0	40.8	34.0	41.0
50-54	37.24085717186894	37.2	35.0	40.0	34.0	41.0
55-59	36.587915266464	36.0	35.0	39.2	33.6	41.0
60-64	35.81849273306246	35.0	34.8	37.4	32.6	40.0
65-69	35.427576585451305	35.0	34.8	36.4	33.0	39.0
70-74	35.20028325680844	35.0	35.0	36.0	33.0	38.0
75-79	34.99350520916297	35.0	34.8	35.2	33.0	37.0
80-84	34.898461914567704	35.0	34.0	35.0	33.0	36.8
85-89	34.751312408775284	35.0	33.8	35.0	32.6	36.2
90-94	39.23468762931792	40.6	38.8	40.6	35.8	40.6
95-99	39.5051637453582	40.8	39.0	41.0	36.0	41.0
100-104	39.40085988648089	40.0	39.0	41.0	36.0	41.0
105-109	39.25675123670946	40.0	39.0	41.0	35.0	41.0
110-114	39.05894314490021	40.0	38.0	41.0	35.0	41.0
115-119	38.79285889846422	40.0	38.0	41.0	34.8	41.0
120-124	38.45537685316805	40.0	37.0	41.0	34.0	41.0
125-129	37.592643961148404	38.8	35.6	40.4	33.2	40.6
130-134	37.54185471370003	38.8	35.0	40.0	33.0	41.0
135-139	37.27988710094063	37.6	35.0	40.0	33.0	41.0
140-144	36.98227625820938	36.8	35.0	40.0	33.0	41.0
145-149	36.367257995632585	35.6	35.0	39.0	33.0	41.0
150-154	35.74601193618824	35.0	35.0	37.2	33.0	39.6
155-159	35.22844428415495	35.0	35.0	36.0	33.0	38.4
160-164	34.83444507550907	35.0	34.4	35.4	33.0	36.8
165-169	34.578494332457566	35.0	34.0	35.0	33.0	36.0
170-174	34.42198200931778	35.0	34.0	35.0	33.0	36.0
175-178	34.176781333963774	35.0	33.75	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	47.0
29	393.0
30	2373.0
31	11671.0
32	42963.0
33	122403.0
34	289657.0
35	669793.0
36	1542247.0
37	2512915.0
38	2373323.0
39	733476.0
40	10630.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.663286770328586	27.716938574274064	28.586776632805144	22.03299802259221
2	22.40157836507019	28.540385269683483	28.363458043006336	20.69457832223999
3	19.580331409503398	26.9703937442883	31.062518617903116	22.38675622830518
4	20.766306876942096	25.343688296238692	31.435622599523672	22.45438222729554
5	22.303044842257336	26.359762614817424	28.733121171449294	22.604071371475953
6	22.060248136044116	26.93154579005598	28.6070247303502	22.401181343549702
7	22.278622003269533	27.007304714738833	27.881846876739978	22.832226405251657
8	22.57750702246853	27.32203450189199	27.445628504316467	22.654829971323014
9	22.41375369169859	27.564819177150042	27.679919325227036	22.341507805924333
10-14	22.62992830031959	27.441242018062795	27.25147294984102	22.67735673177659
15-19	22.618927194915432	27.335711291725158	27.38386639287421	22.661495120485203
20-24	22.63217327655364	27.195767221229534	27.42809699644798	22.743962505768845
25-29	22.726183160223933	27.074487974579075	27.221359469059514	22.977969396137485
30-34	22.735110128957402	27.128826986683656	27.186402325728004	22.949660558630935
35-39	22.77974256643373	27.153805655800145	27.102683721107056	22.963768056659063
40-44	22.87883432556631	27.0319272675824	26.99321887243001	23.096019534421288
45-49	22.984995473954665	26.98288187575103	27.02774290137552	23.004379748918776
50-54	23.01431250550416	27.04375610270201	26.981811120741224	22.96012027105261
55-59	22.974379359115833	26.99814675166617	26.95826654148057	23.069207347737432
60-64	22.90522061643727	27.043847537961273	27.029027807387294	23.021904038214164
65-69	22.947959381570403	27.063903140223672	26.99069237184506	22.99744510636086
70-74	22.905894349926587	27.03248550390212	26.948728400224642	23.11289174594665
75-79	22.84756346689779	27.05588330550974	27.037803186085668	23.058750041506794
80-84	22.867376043865825	27.095558989457512	26.97784090553631	23.059224061140352
85-89	22.84377852840244	27.05222108275709	26.920135632176162	23.183864756664306
90-94	21.433708861918667	27.147930970705353	29.25263830882536	22.16572185855062
95-99	22.24245557528373	27.390704535028814	27.69710739537354	22.66973249431392
100-104	22.535308208449905	27.553984713859	27.23534318495134	22.675363892739757
105-109	22.518823577455624	27.38752300788571	27.290813454167463	22.8028399604912
110-114	22.519837281957855	27.231056185417913	27.36885835222127	22.880248180402965
115-119	22.62062055496105	27.30519614712592	27.157951231537847	22.916232066375184
120-124	22.64289296809331	27.256421601848977	27.04020450912001	23.0604809209377
125-129	22.584585630003108	27.25370935360385	27.123945552180277	23.03775946421277
130-134	22.785187450995615	27.2302132199498	26.97646234829312	23.008136980761464
135-139	22.898647887329656	27.16635840716805	26.880687195055597	23.054306510446697
140-144	22.818703752756285	27.24294593753517	27.038538114857896	22.89981219485065
145-149	22.785681709312115	27.34931770355819	27.038230577014037	22.82677001011566
150-154	22.72462629956319	27.35341463201717	27.070417213031035	22.851541855388604
155-159	22.648627177667503	27.438077481451362	27.15915464810944	22.754140692771692
160-164	22.588361925355045	27.557249606156564	27.193042104734673	22.66134636375372
165-169	22.48743935541139	27.589144236372714	27.24625648330294	22.67715992491296
170-174	22.373041584512052	27.695167073156597	27.395334309996137	22.536457032335218
175-178	22.324228143113057	27.844707104751905	27.32549910911909	22.505565643015952
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.0
4	1.5
5	3.0
6	6.0
7	14.0
8	24.0
9	35.0
10	75.0
11	143.5
12	270.0
13	503.5
14	871.5
15	1540.5
16	2494.5
17	4006.0
18	6414.5
19	9593.5
20	13848.0
21	19479.5
22	26649.5
23	34884.5
24	44243.5
25	54667.5
26	65515.0
27	77208.5
28	89186.0
29	101126.0
30	113429.0
31	126309.5
32	139568.5
33	153997.0
34	169494.0
35	185715.5
36	202994.5
37	220862.5
38	239035.5
39	256297.0
40	271976.5
41	286160.5
42	297562.5
43	304951.0
44	308637.5
45	308602.0
46	303538.0
47	294323.0
48	282757.0
49	269340.5
50	254360.0
51	238423.5
52	223716.5
53	210373.0
54	198033.5
55	187381.0
56	177447.5
57	168722.5
58	161665.0
59	154139.0
60	146297.5
61	137543.0
62	127618.0
63	116514.5
64	103678.5
65	90216.5
66	76655.5
67	63473.5
68	50898.5
69	39659.5
70	30095.5
71	22146.0
72	15811.0
73	10884.0
74	7215.0
75	4648.5
76	2901.0
77	1738.5
78	997.5
79	557.5
80	296.5
81	139.5
82	69.5
83	36.5
84	16.5
85	7.5
86	3.5
87	0.0
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1313.0
92-93	2985.0
94-95	4182.0
96-97	5147.0
98-99	6261.0
100-101	7366.0
102-103	8269.0
104-105	9191.0
106-107	10015.0
108-109	10998.0
110-111	11671.0
112-113	12513.0
114-115	13054.0
116-117	13632.0
118-119	14606.0
120-121	15715.0
122-123	15587.0
124-125	16359.0
126-127	17260.0
128-129	17642.0
130-131	18857.0
132-133	20623.0
134-135	113081.0
136-137	118170.0
138-139	91335.0
140-141	93371.0
142-143	90947.0
144-145	85236.0
146-147	88174.0
148-149	83164.0
150-151	106298.0
152-153	101205.0
154-155	86373.0
156-157	84039.0
158-159	85194.0
160-161	88076.0
162-163	87655.0
164-165	90229.0
166-167	91813.0
168-169	96604.0
170-171	95656.0
172-173	102029.0
174-175	104382.0
176-177	222727.0
178-179	5852888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.4102435345992
#Duplication Level	Percentage of total
1	93.13524425033903
2	6.055791657276692
3	0.6080743132683684
4	0.1292803340432621
5	0.043014922100575566
6	0.014425588489403156
7	0.004146633918909194
8	0.003128828138329428
9	0.0
>10	0.0010876298487576173
>50	0.0
>100	0.005805842576691666
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2030955166404953E-5	1.2030955166404953E-5	0.0	0.0	0.0
2	0.0	2.4061910332809906E-5	2.4061910332809906E-5	2.4061910332809906E-5	0.0	0.0
3	2.4061910332809906E-5	2.4061910332809906E-5	3.609286549921486E-5	3.609286549921486E-5	1.2030955166404953E-5	0.0
4	2.4061910332809906E-5	2.4061910332809906E-5	6.0154775832024764E-5	7.218573099842972E-5	1.2030955166404953E-5	0.0
5	2.4061910332809906E-5	2.4061910332809906E-5	6.0154775832024764E-5	8.421668616483468E-5	1.2030955166404953E-5	0.0
6	2.4061910332809906E-5	3.609286549921486E-5	7.218573099842972E-5	9.624764133123962E-5	1.2030955166404953E-5	0.0
7	2.4061910332809906E-5	3.609286549921486E-5	7.218573099842972E-5	9.624764133123962E-5	1.2030955166404953E-5	0.0
8	2.4061910332809906E-5	3.609286549921486E-5	7.218573099842972E-5	9.624764133123962E-5	2.4061910332809906E-5	0.0
9	2.4061910332809906E-5	3.609286549921486E-5	7.218573099842972E-5	1.0827859649764458E-4	2.4061910332809906E-5	0.0
10-14	2.4061910332809906E-5	6.496715789858673E-5	1.1309097856420656E-4	1.564024171632644E-4	5.05300116989008E-5	0.0
15-19	3.1280483432652876E-5	1.0827859649764457E-4	1.6843337232966935E-4	1.8768290059591728E-4	6.256096686530575E-5	0.0
20-24	4.331143859905783E-5	1.7083956336295033E-4	2.334005302282561E-4	1.9249528266247924E-4	7.459192203171071E-5	0.0
25-29	4.812382066561981E-5	2.045262378288842E-4	2.59868631594347E-4	1.973076647290412E-4	9.86538323645206E-5	0.0
30-34	7.699811306499169E-5	2.62274822627628E-4	3.0077387916012377E-4	2.3099433919497513E-4	1.3715288889701646E-4	7.218573099842972E-6
35-39	1.2030955166404953E-4	2.791181598605949E-4	3.5130389085902465E-4	2.887429239937189E-4	1.9008909162919826E-4	2.4061910332809902E-5
40-44	1.8046432749607428E-4	3.3686674465933876E-4	3.7536580119183456E-4	3.0799245225996677E-4	2.2858814816169412E-4	3.609286549921486E-5
45-49	2.4543148539466104E-4	3.561162729255866E-4	4.1386485772433037E-4	3.6092865499214857E-4	3.0318007019340486E-4	6.737334893186774E-5
50-54	2.59868631594347E-4	3.7777199222511555E-4	4.403329590904214E-4	3.8258437429167745E-4	3.609286549921486E-4	1.1068478753092556E-4
55-59	3.128048343265288E-4	4.307081949572973E-4	4.6439486942323114E-4	4.018339025579254E-4	4.090524756577684E-4	1.3715288889701646E-4
60-64	3.5130389085902465E-4	4.7161344252307413E-4	4.908629707893221E-4	4.619886783899502E-4	4.5477010529010727E-4	1.4918384406342142E-4
65-69	3.705534191252726E-4	5.17331072155413E-4	5.26955836288537E-4	5.07706308022289E-4	5.028939259557272E-4	1.9490147369576023E-4
70-74	6.953892086182063E-4	5.534239376546278E-4	5.750796569541567E-4	5.437991735215039E-4	5.341744093883799E-4	2.2377576609513213E-4
75-79	0.004631917739065907	5.798920390207187E-4	6.207972865864955E-4	6.015477583202475E-4	5.750796569541567E-4	2.4302529436138006E-4
80-84	0.006220003821031361	6.135787134866526E-4	6.448591969193055E-4	6.520777700191485E-4	7.266696920508592E-4	2.5265005849450404E-4
85-89	0.0062994081251296335	6.689211072521154E-4	6.544839610524295E-4	6.881706355183634E-4	0.0010130064250112969	2.5265005849450404E-4
90-94	0.006345125754761973	7.218573099842971E-4	6.833582534518014E-4	7.60356366516793E-4	0.001046693099477231	2.5265005849450404E-4
95-99	0.0063691876650947814	7.89230658916165E-4	7.122325458511732E-4	8.445730526816277E-4	0.001058724054643636	2.5265005849450404E-4
100-104	0.006393249575427593	8.349482885485037E-4	7.483254113503882E-4	8.566040078480327E-4	0.001090004538076289	2.5265005849450404E-4
105-109	0.00641249910369384	9.047278285136525E-4	7.62762557550074E-4	9.071340195469334E-4	0.0011140664484090988	2.5265005849450404E-4
110-114	0.006426936249893526	9.504454581459913E-4	8.229173333820988E-4	9.745073684788013E-4	0.0011333159766753465	2.5265005849450404E-4
115-119	0.0064582167333261786	9.528516491792722E-4	8.541978168147517E-4	0.001022631189144421	0.0011525655049415945	2.5265005849450404E-4
120-124	0.006489497216758832	9.889445146784872E-4	8.710411540477187E-4	0.0010707550098100406	0.0011838459883742477	2.5986863159434693E-4
125-129	0.006578526284990229	0.0010178188070778592	9.263835478131813E-4	0.0011260974035755037	0.0011982831345739335	2.6468101366090895E-4
130-134	0.006614619150489444	0.0010274435712109829	9.408206940128673E-4	0.0011621902690747185	0.001244000764206272	2.6708720469418993E-4
135-139	0.006657930589088501	0.0010490992905105119	9.576640312458343E-4	0.00126325029247252	0.0013209988772712638	2.8874292399371887E-4
140-144	0.006732522511120212	0.0010779735829098839	9.961630877783302E-4	0.001301749349005016	0.0013474669786373546	3.007738791601238E-4
145-149	0.0068023020510853596	0.0011068478753092556	0.001034662144310826	0.0013378422145042307	0.0013907784172364128	3.031800701934048E-4
150-154	0.006879300164150353	0.0011309097856420655	0.0010515054815437928	0.0013787474620700077	0.0014437146199685943	3.128048343265288E-4
155-159	0.006912986838616286	0.0011309097856420655	0.0010611302456769167	0.0014100279455026604	0.0015062755868339001	3.128048343265288E-4
160-164	0.006973141614448311	0.0011309097856420655	0.0010851921560097269	0.0014581517661682805	0.0015808675088656108	3.296481715594957E-4
165-167	0.007014046862014088	0.0011429407408084706	0.0010988272385316522	0.001479807485467809	0.0016402202210198753	3.4087706304814034E-4
>>END_MODULE
