##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479504
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6591052
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.21120103437205	39.0	39.0	39.0	37.0	39.0
2	39.92636820343702	41.0	40.0	41.0	38.0	41.0
3	39.972758369984035	41.0	40.0	41.0	38.0	41.0
4	39.994197739602114	41.0	40.0	41.0	38.0	41.0
5	39.998747999560614	41.0	40.0	41.0	38.0	41.0
6	39.989947280039665	41.0	40.0	41.0	38.0	41.0
7	40.017423318766106	41.0	40.0	41.0	38.0	41.0
8	39.984632650447914	41.0	40.0	41.0	38.0	41.0
9	39.94610738923013	41.0	40.0	41.0	38.0	41.0
10-14	39.888711453042696	41.0	40.0	41.0	38.0	41.0
15-19	39.74418563227842	41.0	39.8	41.0	37.6	41.0
20-24	39.54763770639345	40.0	39.0	41.0	36.8	41.0
25-29	39.46319528354503	40.0	39.0	41.0	36.4	41.0
30-34	39.35059155958715	40.0	38.8	41.0	35.6	41.0
35-39	38.989248332436155	40.0	38.0	41.0	35.0	41.0
40-44	38.470783176949595	39.8	36.6	41.0	34.6	41.0
45-49	38.051105680853375	39.0	35.6	41.0	34.4	41.0
50-54	37.48689480829464	37.8	35.0	40.4	34.0	41.0
55-59	36.80786824318788	36.4	35.0	39.4	34.0	41.0
60-64	36.030551329287036	35.4	34.8	38.2	33.2	40.2
65-69	35.61157979029751	35.0	34.8	36.8	33.0	39.0
70-74	35.338191869825934	35.0	35.0	36.0	33.0	38.6
75-79	35.12018442579424	35.0	35.0	35.6	33.0	37.0
80-84	35.029582136508715	35.0	35.0	35.0	33.0	37.0
85-89	34.796010348575614	34.8	34.6	35.0	32.2	38.0
90-94	39.48372186630725	40.6	39.8	40.6	37.0	40.6
95-99	39.725696566947434	41.0	40.0	41.0	37.0	41.0
100-104	39.602231389295284	41.0	39.0	41.0	36.6	41.0
105-109	39.43960613149643	40.4	39.0	41.0	36.0	41.0
110-114	39.22951965506964	40.0	38.4	41.0	35.2	41.0
115-119	38.97244935269872	40.0	38.0	41.0	35.0	41.0
120-124	38.954781759606774	40.0	38.0	41.0	35.0	41.0
125-129	38.44933256108399	39.6	36.8	40.6	34.6	41.0
130-134	38.17849953001252	39.4	35.8	41.0	34.0	41.0
135-139	37.678204198885	38.6	35.0	40.8	34.0	41.0
140-144	37.05715823685916	37.2	35.0	40.0	33.4	41.0
145-149	36.41563012325133	36.0	35.0	39.0	33.0	41.0
150-154	35.97921607440873	35.0	35.0	37.6	33.8	39.8
155-159	35.410393430137646	35.0	35.0	36.4	33.0	38.4
160-164	34.97957802990476	35.0	35.0	35.6	33.0	36.8
165-169	34.70303602192537	35.0	35.0	35.0	33.0	36.0
170-174	34.548899437376356	35.0	35.0	35.0	33.0	36.0
175-178	34.159557160673316	34.75	33.5	35.0	32.0	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	33.0
30	363.0
31	2929.0
32	15816.0
33	61143.0
34	182341.0
35	448716.0
36	1043772.0
37	1958194.0
38	2182985.0
39	686620.0
40	8139.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.40432210214697	28.02587508033619	29.119615502957647	21.450187314559194
2	22.086641100692272	28.425128492386342	28.75973365101656	20.728496755904825
3	20.201251636309348	27.885942942037172	30.184862750286296	21.72794267136718
4	20.86650203943164	26.97910743231885	30.446459836760503	21.707930691489004
5	21.595035208340036	27.719004492757758	28.833363778650206	21.852596520252003
6	21.454981693362456	28.018182833332222	28.84559247901549	21.681242994289835
7	21.517172069041482	28.05955710863759	28.47241988077169	21.95085094154924
8	21.684808434222642	28.182754437379646	28.271662854427486	21.860774273970225
9	21.719992498921265	28.32423412833035	28.32874023752202	21.627033135226366
10-14	21.755774343761818	28.070107776421732	28.204920853302323	21.969197026514127
15-19	21.879530005225266	27.934508785547436	28.152753156855688	22.03320805237161
20-24	21.91761497254156	27.836230088914487	28.086763691137623	22.159391247406333
25-29	22.03339315180642	27.795835930288522	27.884343804297096	22.286427113607964
30-34	22.041998758316577	27.853723502712462	27.890263951794037	22.214013787176917
35-39	22.008158940333043	27.8650691877412	27.892418387838543	22.23435348408721
40-44	22.084031502103155	27.81945886635396	27.770853575423164	22.325656056119723
45-49	22.273743250698068	27.733269286905944	27.7068577216505	22.286129740745483
50-54	22.25198648106554	27.792334213111957	27.724496787462762	22.23118251835974
55-59	22.203460084975813	27.69949926051258	27.733281424573804	22.363759229937802
60-64	22.185702676901958	27.778491202921778	27.745910667978347	22.289895452197918
65-69	22.139946703500442	27.74970065476649	27.787998031270273	22.322354610462792
70-74	22.122656595639057	27.746930232078277	27.758964729757864	22.371448442524805
75-79	22.063600772683937	27.744545180344502	27.822754243176963	22.369099803794597
80-84	22.062687413177745	27.767327582910895	27.80032686739537	22.36965813651599
85-89	22.048540961291156	27.689352170184666	27.77225244164361	22.489854426880566
90-94	21.271671384826178	27.853748982987824	29.239143627416208	21.635436004769787
95-99	21.58795650124778	28.167770152433313	28.331219736672526	21.913053609646376
100-104	21.76918209717132	28.0529701601143	28.162644330986492	22.01520341172789
105-109	21.827194485478508	27.94340262350098	28.08192162166722	22.14748126935329
110-114	21.90709589522473	27.83083271153907	28.0334064670614	22.228664926174798
115-119	22.007153067261918	27.87320862555582	27.850691812127852	22.26894649505441
120-124	22.010277567777525	27.854058512745784	27.83166135774587	22.304002561730822
125-129	21.986793837215586	27.87398768820118	27.867005931559376	22.272212543023866
130-134	22.086654361476068	27.906208778821394	27.73856014185475	22.268576717847786
135-139	22.165355313386037	27.852988844997757	27.6880472188049	22.293608622811306
140-144	22.122745458728737	27.955726363437755	27.73472699935155	22.18680117848196
145-149	22.057260952514884	28.00480366472733	27.77024081771893	22.167694565038857
150-154	22.006907614816924	28.05967727173248	27.8134897208518	22.119925392598798
155-159	21.92638818554006	28.082132505100983	27.937571678914907	22.05390763044405
160-164	21.90707903896632	28.19544138637628	27.922909152691645	21.97457042196576
165-169	21.797266833612415	28.19270410819634	28.00453819294763	22.005490865243612
170-174	21.76821341558691	28.315178877244897	28.05497758939447	21.86163011777373
175-178	21.709263536426207	28.37796860633004	28.00142365353861	21.911344203705145
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	6.5
10	18.5
11	48.5
12	113.5
13	224.5
14	434.5
15	754.5
16	1226.0
17	2005.5
18	3167.0
19	4953.0
20	7358.5
21	10617.5
22	15036.5
23	20619.0
24	27580.0
25	35902.0
26	45702.0
27	57140.0
28	69609.0
29	82939.0
30	98011.5
31	113613.0
32	129555.0
33	146580.5
34	163263.0
35	180207.0
36	197274.0
37	213392.0
38	228953.5
39	242455.5
40	253318.5
41	262225.5
42	268699.5
43	271065.0
44	270754.5
45	266584.5
46	257996.5
47	247246.5
48	234119.5
49	219131.5
50	202516.0
51	185589.5
52	169001.0
53	153246.5
54	138896.0
55	125999.5
56	114594.5
57	104920.5
58	96572.0
59	88792.5
60	81313.5
61	74426.0
62	67643.0
63	60737.5
64	53745.0
65	46562.0
66	39726.0
67	33081.0
68	26792.5
69	21253.0
70	16457.0
71	12426.5
72	9086.0
73	6437.5
74	4388.0
75	2907.5
76	1902.5
77	1180.5
78	713.0
79	426.0
80	253.0
81	142.0
82	75.5
83	37.0
84	15.0
85	9.0
86	4.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1044.0
92-93	2454.0
94-95	3601.0
96-97	4891.0
98-99	6010.0
100-101	7342.0
102-103	8321.0
104-105	9152.0
106-107	10077.0
108-109	10785.0
110-111	11680.0
112-113	12278.0
114-115	12608.0
116-117	13386.0
118-119	14166.0
120-121	15008.0
122-123	15309.0
124-125	16679.0
126-127	16406.0
128-129	16829.0
130-131	18579.0
132-133	20119.0
134-135	79498.0
136-137	77914.0
138-139	77217.0
140-141	80100.0
142-143	79377.0
144-145	83268.0
146-147	87001.0
148-149	70242.0
150-151	67025.0
152-153	69764.0
154-155	68320.0
156-157	78164.0
158-159	72526.0
160-161	71503.0
162-163	70075.0
164-165	72355.0
166-167	77948.0
168-169	86272.0
170-171	82057.0
172-173	86331.0
174-175	88753.0
176-177	284870.0
178-179	4433748.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.99447935567315
#Duplication Level	Percentage of total
1	92.25275275695083
2	7.038751322044816
3	0.5895185624696027
4	0.07463432999707578
5	0.024723671653455865
6	0.008303652796869286
7	0.0020915978286577433
8	0.0010536799179441752
9	0.0021231991937403095
>10	0.0022200118455716713
>50	0.001636663009316385
>100	0.002190552292104582
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.586042410225257E-5	0.0	0.0	6.0688339281802054E-5	0.0	0.0
2	4.551625446135154E-4	0.0	0.0	9.103250892270308E-5	0.0	0.0
3	4.551625446135154E-4	0.0	1.5172084820450513E-5	9.103250892270308E-5	0.0	0.0
4	4.551625446135154E-4	1.5172084820450513E-5	1.5172084820450513E-5	1.2137667856360411E-4	1.5172084820450513E-5	0.0
5	4.551625446135154E-4	3.0344169640901027E-5	4.551625446135154E-5	1.3654876338405463E-4	1.5172084820450513E-5	0.0
6	4.551625446135154E-4	4.551625446135154E-5	4.551625446135154E-5	1.5172084820450513E-4	1.5172084820450513E-5	0.0
7	4.551625446135154E-4	4.551625446135154E-5	4.551625446135154E-5	1.5172084820450513E-4	1.5172084820450513E-5	0.0
8	4.703346294339659E-4	4.551625446135154E-5	4.551625446135154E-5	1.5172084820450513E-4	1.5172084820450513E-5	0.0
9	4.703346294339659E-4	4.551625446135154E-5	4.551625446135154E-5	1.5172084820450513E-4	1.5172084820450513E-5	0.0
10-14	4.7336904639805604E-4	4.551625446135154E-5	4.551625446135154E-5	1.7599618391722597E-4	1.5172084820450513E-5	0.0
15-19	4.8550671425441643E-4	4.551625446135154E-5	5.461950535362185E-5	2.488221910553884E-4	1.5172084820450513E-5	0.0
20-24	4.915755481825967E-4	6.0688339281802054E-5	8.799809195861299E-5	3.3075144908582116E-4	1.5172084820450513E-5	0.0
25-29	5.31022968715768E-4	9.103250892270308E-5	9.406692588679318E-5	4.035774562239837E-4	3.0344169640901027E-5	0.0
30-34	5.674359722848492E-4	1.031701767790635E-4	1.153078446354239E-4	4.855067142544165E-4	6.0688339281802054E-5	1.213766785636041E-5
35-39	6.463308133511918E-4	1.062045937431536E-4	1.3654876338405463E-4	6.008145588898403E-4	1.031701767790635E-4	1.5172084820450513E-5
40-44	7.37363322273895E-4	1.1834226159951401E-4	1.5172084820450513E-4	6.675717320998225E-4	1.4565201427632493E-4	3.0344169640901027E-5
45-49	8.132237463761476E-4	1.4565201427632493E-4	1.7903060088131606E-4	7.98051661555697E-4	1.7599618391722597E-4	6.0688339281802054E-5
50-54	8.799809195861298E-4	1.5172084820450513E-4	2.124091874863072E-4	8.769465026220398E-4	2.9433844551674E-4	8.496367499452289E-5
55-59	9.224627570833912E-4	1.6689293302495566E-4	2.275812723067577E-4	9.710134285088329E-4	4.460592937212451E-4	1.3351434641996454E-4
60-64	9.619101776165626E-4	1.6689293302495566E-4	2.6095985891174886E-4	0.0010772180222519865	5.158508838953175E-4	1.7599618391722597E-4
65-69	0.0010104608490420042	1.7296176695313586E-4	3.0040727944492015E-4	0.001125768693677428	5.947457249616602E-4	1.9723710266585665E-4
70-74	0.001326040213307375	1.8206501784540617E-4	3.4895795087036183E-4	0.0011591472802824191	6.432963963871018E-4	2.245468553426676E-4
75-79	0.0069063330102690735	1.8206501784540617E-4	4.157151240803441E-4	0.0012319732874205816	6.827438169202732E-4	2.3971894016311816E-4
80-84	0.009082009973521677	1.8206501784540617E-4	4.369560428289748E-4	0.0013381778811637356	8.162581633402376E-4	2.5792544194765875E-4
85-89	0.009103250892270308	1.9420268570176656E-4	4.490937106853352E-4	0.0014019006374096274	0.0011652161142105995	2.5792544194765875E-4
90-94	0.009236765238690273	2.2151243837857751E-4	4.824722972903263E-4	0.0014898987293682406	0.0011985947008155905	2.5792544194765875E-4
95-99	0.009309591245828435	2.488221910553884E-4	5.037132160389572E-4	0.0015718279873986733	0.0012289388704564916	2.5792544194765875E-4
100-104	0.009361176334217968	2.8523519462446965E-4	5.158508838953175E-4	0.0016446539945368355	0.001241076538312852	2.5792544194765875E-4
105-109	0.00939758933778705	2.9130402855264987E-4	5.461950535362185E-4	0.0017569274222081696	0.0012562486231333023	2.5792544194765875E-4
110-114	0.00943703675832022	3.0344169640901026E-4	5.461950535362185E-4	0.001850994348094963	0.0012805239588460232	2.6095985891174886E-4
115-119	0.009440071175284311	3.0344169640901026E-4	5.674359722848492E-4	0.0019359580230894856	0.0013047992945587443	2.7309752676810925E-4
120-124	0.009503793931530202	3.0344169640901026E-4	5.795736401412097E-4	0.0020269905320121884	0.0013412122981278254	2.7613194373219936E-4
125-129	0.009606964108309265	3.0344169640901026E-4	6.038489758539305E-4	0.002093747705222171	0.0013654876338405463	2.973728624808301E-4
130-134	0.009682824532411517	3.1861378122946077E-4	6.159866437102908E-4	0.002181745797180784	0.0013654876338405463	3.368202830140014E-4
135-139	0.009816338878831482	3.307514490858212E-4	6.372275624589215E-4	0.002215124383785775	0.0013806597186609968	3.944742053317133E-4
140-144	0.009916474638646457	3.610956187267222E-4	6.402619794230117E-4	0.0022545718043189464	0.0014231415561582583	4.248183749726144E-4
145-149	0.009995369479712798	3.6413003569081234E-4	6.736405660280029E-4	0.002336501062349379	0.001453485725799159	4.399904597930649E-4
150-154	0.010083367571671411	3.6413003569081234E-4	6.979159017407237E-4	0.0024002238185952705	0.00148382989544006	4.399904597930649E-4
155-159	0.010198675416306837	3.7019886961899256E-4	7.495009901302554E-4	0.0024669809918052535	0.0015475526516859524	4.460592937212451E-4
160-164	0.010320052094870441	3.7930212051126284E-4	7.889484106634266E-4	0.0025458758328715963	0.001623413075788205	4.703346294339659E-4
165-167	0.010402992825222236	3.7930212051126284E-4	7.889484106634267E-4	0.0025640823346561366	0.0016638719686427397	4.905640758612333E-4
>>END_MODULE
