##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479520
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7776766
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-177
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.74397275165538	41.0	40.0	41.0	38.0	41.0
2	39.90159842793264	41.0	40.0	41.0	38.0	41.0
3	39.962346558968086	41.0	40.0	41.0	38.0	41.0
4	39.98494155539719	41.0	40.0	41.0	38.0	41.0
5	39.990521381252826	41.0	40.0	41.0	38.0	41.0
6	40.010764757484026	41.0	40.0	41.0	38.0	41.0
7	39.978675583140856	41.0	40.0	41.0	38.0	41.0
8	39.94626262382075	41.0	40.0	41.0	38.0	41.0
9	39.92892945473736	41.0	40.0	41.0	38.0	41.0
10-14	39.857863975848055	41.0	40.0	41.0	38.0	41.0
15-19	39.68675153656417	41.0	39.4	41.0	37.2	41.0
20-24	39.45237588478295	40.0	39.0	41.0	36.4	41.0
25-29	39.288994962687575	40.0	38.6	41.0	35.6	41.0
30-34	38.94072700657317	40.0	37.8	41.0	35.0	41.0
35-39	38.372627824985344	39.6	36.4	41.0	34.6	41.0
40-44	37.65286148509547	38.6	35.0	40.6	33.8	41.0
45-49	37.14377153690879	37.2	35.0	40.0	34.0	41.0
50-54	36.496880657075195	35.6	35.0	39.0	33.4	41.0
55-59	35.89063685341696	35.0	35.0	37.4	33.0	40.0
60-64	35.25798150542269	35.0	34.6	36.4	32.6	39.0
65-69	35.174922660653536	35.0	35.0	36.0	33.0	38.4
70-74	35.0496319164033	35.0	35.0	35.0	33.0	37.0
75-79	34.99351195599816	35.0	35.0	35.0	33.0	37.6
80-84	34.99060560649504	35.0	34.8	35.0	33.0	38.0
85-89	35.47076113644155	35.8	34.8	35.8	32.8	39.2
90-94	39.6339362276305	41.0	39.0	41.0	37.0	41.0
95-99	39.544238852267284	41.0	39.0	41.0	36.0	41.0
100-104	39.385006375447574	40.8	39.0	41.0	35.6	41.0
105-109	39.185235912949494	40.0	38.4	41.0	35.0	41.0
110-114	38.922560795056945	40.0	38.0	41.0	35.0	41.0
115-119	38.61410348826247	40.0	37.4	41.0	34.4	41.0
120-124	38.38135798981772	40.0	36.4	41.0	34.0	41.0
125-129	37.68575823459634	38.8	35.0	40.4	33.4	41.0
130-134	37.27776282146591	37.6	35.0	40.4	33.0	41.0
135-139	36.63989149831558	36.4	35.0	39.6	33.0	41.0
140-144	35.98932643352044	35.0	35.0	38.4	33.0	40.6
145-149	35.46781034311041	35.0	34.8	36.8	33.0	39.2
150-154	35.14682367972652	35.0	35.0	35.8	33.0	37.8
155-159	34.78617840792255	35.0	35.0	35.0	33.0	36.6
160-164	34.55273931102492	35.0	34.6	35.0	33.0	36.0
165-169	34.415443985740836	35.0	34.0	35.0	33.0	35.4
170-174	34.34011907347914	35.0	34.0	35.0	33.0	35.0
175-177	33.89627728421547	34.666666666666664	33.333333333333336	35.0	31.666666666666668	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	2.0
29	62.0
30	584.0
31	4220.0
32	22925.0
33	88654.0
34	271570.0
35	714465.0
36	1737820.0
37	2697472.0
38	1953421.0
39	284146.0
40	1424.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.13670849810834	25.718711351222346	25.929840244646684	23.214739906022633
2	22.883882580496827	25.1565367917718	27.260483342304497	24.69909728542687
3	23.38812817564525	24.44446701880962	27.63188451343399	24.535520292111144
4	24.43164677965108	24.974520256877987	25.928400057298884	24.665432906172054
5	24.247482822551174	25.308232239468182	25.937748416243977	24.50653652173667
6	24.180874157715433	25.377489820318626	25.79232807056301	24.649307951402935
7	24.449983450704316	25.423254345058087	25.417995089475497	24.7087671147621
8	24.510561331021147	25.631104240503056	25.471269162528486	24.38706526594731
9	24.210629457026226	25.513973289153874	25.598892907411642	24.67650434640826
10-14	24.507225754253117	25.40098544819273	25.436429487527334	24.655359310026817
15-19	24.49335623574118	25.284785475093376	25.45135085715579	24.770507432009655
20-24	24.512232977049845	25.19514924327156	25.437969973636854	24.85464780604174
25-29	24.653201600768238	25.250056385906426	25.22791864896025	24.868823364365085
30-34	24.56454263893243	25.23069872489413	25.284613166964263	24.920145469209178
35-39	24.52516637378571	25.26531980002999	25.323508512407344	24.88600531377696
40-44	24.69805829312596	25.237318443167766	25.123119301776597	24.941503961929676
45-49	24.856892955246433	25.118333250608288	25.073517706460503	24.95125608768478
50-54	24.828420965733056	25.177923573886623	25.119719430930544	24.873936029449776
55-59	24.812092841677376	25.178021300885227	25.085486692025967	24.924399165411433
60-64	24.742961277219862	25.175387815449252	25.134131591461028	24.94751931586986
65-69	24.70082036671799	25.17560641531454	25.199328358343305	24.924244859624167
70-74	24.71490848509522	25.211899136479097	25.148600330780173	24.924592047645515
75-79	24.6399544489316	25.154584823562903	25.171530170767642	25.033930556737854
80-84	24.63349675173459	25.225326311734207	25.178743966322248	24.962432970208955
85-89	24.51448326978078	25.216664613542445	25.29658986781909	24.97226224885769
90-94	23.969061032836073	25.181527882389982	26.366854655216454	24.482556429557494
95-99	24.369582823992186	25.50867909068661	25.45280607298111	24.668932012340097
100-104	24.446061696165195	25.398268632865097	25.4066378588102	24.74903181215951
105-109	24.469458582191876	25.349700307346374	25.400493074803325	24.780348035658424
110-114	24.51469574940541	25.22824921546272	25.298496223864042	24.95855881126782
115-119	24.591465098969444	25.303605527207964	25.1846042071805	24.92032516664209
120-124	24.555668809120217	25.325006811115315	25.20854999040613	24.910774389358334
125-129	24.5573799867488	25.306315127380586	25.177459632958566	24.958845252912052
130-134	24.70181761212271	25.28017562608505	25.09917357402988	24.91883318776236
135-139	24.816014466851243	25.320867756628086	25.0121169030323	24.85100087348837
140-144	24.73572253596795	25.366577195805974	25.02377620030034	24.87392406792574
145-149	24.657226917467227	25.440299563090395	25.113622299463177	24.7888512199792
150-154	24.58936903609643	25.55597176432104	25.193005288967324	24.661653910615208
155-159	24.522365030978065	25.52828522140627	25.246913752475216	24.702435995140444
160-164	24.48551616654778	25.639823498880045	25.29633466655194	24.57832566802024
165-169	24.428195183861945	25.69133419462675	25.334477472816985	24.545993148694315
170-174	24.332523033120893	25.767085418529074	25.38028499639023	24.520106551959806
175-177	24.382139487645933	25.81719085314725	25.249530183586433	24.55113947562038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.5
8	0.5
9	0.0
10	0.0
11	0.5
12	4.5
13	11.0
14	25.5
15	48.5
16	71.0
17	113.5
18	213.5
19	356.5
20	593.0
21	975.5
22	1548.0
23	2325.0
24	3478.5
25	5180.0
26	7514.5
27	10650.5
28	14763.5
29	20197.5
30	27414.0
31	36811.5
32	48692.0
33	63262.0
34	81128.5
35	102001.0
36	125614.5
37	151114.5
38	177397.5
39	203805.5
40	228390.0
41	250738.5
42	270035.5
43	285439.5
44	296293.0
45	302853.0
46	305735.0
47	304917.0
48	301702.0
49	295752.5
50	288228.5
51	280511.0
52	272701.0
53	264660.5
54	256777.5
55	248594.5
56	239606.5
57	229976.5
58	218479.0
59	205632.0
60	192188.0
61	178171.5
62	162350.0
63	145514.5
64	128882.5
65	111694.0
66	94531.0
67	78366.5
68	63933.0
69	50930.5
70	39575.5
71	30183.5
72	22188.0
73	15795.0
74	11040.5
75	7519.0
76	4977.0
77	3231.5
78	1995.0
79	1169.0
80	688.5
81	384.5
82	203.0
83	104.0
84	47.5
85	21.0
86	10.0
87	5.5
88	2.0
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-177	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1754.0
92-93	3853.0
94-95	5779.0
96-97	7900.0
98-99	9920.0
100-101	11635.0
102-103	13437.0
104-105	14789.0
106-107	15984.0
108-109	17964.0
110-111	18721.0
112-113	19817.0
114-115	20534.0
116-117	21459.0
118-119	23167.0
120-121	23268.0
122-123	24511.0
124-125	26281.0
126-127	26725.0
128-129	27803.0
130-131	29126.0
132-133	61613.0
134-135	121726.0
136-137	121169.0
138-139	123089.0
140-141	121157.0
142-143	122334.0
144-145	126102.0
146-147	116294.0
148-149	99431.0
150-151	99534.0
152-153	124651.0
154-155	101219.0
156-157	104975.0
158-159	101918.0
160-161	101586.0
162-163	103928.0
164-165	105680.0
166-167	112793.0
168-169	120370.0
170-171	120726.0
172-173	120686.0
174-175	131864.0
176-177	4949494.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.72782807055744
#Duplication Level	Percentage of total
1	93.65332342786758
2	5.825789511328243
3	0.42221623015247617
4	0.05910421243903232
5	0.01640952177968092
6	0.011353978220326704
7	0.0020775846426525084
8	0.0010454325545264835
9	0.0021042010616714475
>10	0.003292237713951971
>50	0.0014204509343249848
>100	0.0018632113055500137
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.5430578726426897E-4	0.0	0.0	0.0	0.0	0.0
2	1.5430578726426897E-4	1.2858815605355749E-5	0.0	0.0	1.2858815605355749E-5	0.0
3	1.5430578726426897E-4	6.429407802677874E-5	2.5717631210711498E-5	1.2858815605355749E-5	1.2858815605355749E-5	0.0
4	1.5430578726426897E-4	7.715289363213449E-5	2.5717631210711498E-5	1.2858815605355749E-5	1.2858815605355749E-5	0.0
5	1.5430578726426897E-4	7.715289363213449E-5	3.8576446816067243E-5	1.2858815605355749E-5	1.2858815605355749E-5	0.0
6	1.6716460286962472E-4	7.715289363213449E-5	5.1435262421422996E-5	1.2858815605355749E-5	1.2858815605355749E-5	0.0
7	1.6716460286962472E-4	7.715289363213449E-5	5.1435262421422996E-5	3.8576446816067243E-5	1.2858815605355749E-5	0.0
8	1.6716460286962472E-4	7.715289363213449E-5	5.1435262421422996E-5	5.1435262421422996E-5	1.2858815605355749E-5	0.0
9	1.6716460286962472E-4	7.715289363213449E-5	5.1435262421422996E-5	5.1435262421422996E-5	1.2858815605355749E-5	0.0
10-14	1.7745165535390932E-4	7.972465675320564E-5	6.429407802677874E-5	6.429407802677874E-5	2.314586808964035E-5	0.0
15-19	2.0574104968569198E-4	1.2858815605355748E-4	8.74399461164191E-5	6.429407802677874E-5	3.8576446816067243E-5	0.0
20-24	2.2117162841211888E-4	1.5430578726426897E-4	1.3373168229569978E-4	7.200936738999219E-5	3.8576446816067243E-5	0.0
25-29	2.3145868089640347E-4	1.7745165535390932E-4	1.8773870783819394E-4	9.001170923749023E-5	3.8576446816067243E-5	0.0
30-34	2.494610227439015E-4	2.0316928656462083E-4	2.3917397025961692E-4	9.001170923749023E-5	4.886349930035185E-5	0.0
35-39	3.0861157452853795E-4	2.1859986529104773E-4	2.8803746955996875E-4	9.77269986007037E-5	1.1830110356927289E-4	3.60046836949961E-5
40-44	3.6004683694996093E-4	2.443174965017592E-4	3.626186000710321E-4	1.2344462981141518E-4	1.4401873477998438E-4	9.001170923749023E-5
45-49	4.0376681000817047E-4	2.803221801967553E-4	3.8833623128174357E-4	1.5173402414319782E-4	1.8002341847498047E-4	1.2087286669034404E-4
50-54	4.2691267809781086E-4	2.8803746955996875E-4	4.269126780978109E-4	1.5430578726426897E-4	2.1602810216997658E-4	1.6202107662748242E-4
55-59	4.7834794051923384E-4	3.0861157452853795E-4	4.500585461874512E-4	1.6202107662748242E-4	2.75178653954613E-4	1.928822340803362E-4
60-64	5.092090979720876E-4	3.0861157452853795E-4	4.860632298824473E-4	1.6973636599069587E-4	3.2918567949710714E-4	2.0059752344354969E-4
65-69	5.477855447881549E-4	3.111833376496091E-4	5.374984923038703E-4	2.0059752344354969E-4	4.1405386249245506E-4	2.2374339153319003E-4
70-74	9.97844090975606E-4	3.3432920573924944E-4	5.863619916042221E-4	2.1859986529104773E-4	4.552020724295935E-4	2.4688925962283037E-4
75-79	0.003873075260333151	3.523315475867475E-4	6.146513859360047E-4	2.3917397025961692E-4	5.117808610931588E-4	2.5717631210711497E-4
80-84	0.004526303093085224	3.703338894342455E-4	6.377972540256451E-4	2.6231983834925726E-4	6.043643334517202E-4	2.5717631210711497E-4
85-89	0.00456230777678022	4.1148209937138397E-4	6.583713589942143E-4	2.9832452204425335E-4	6.815172270838546E-4	2.6746336459139956E-4
90-94	0.004672893590986278	4.5005854618745115E-4	6.789454639627836E-4	3.2404215325496484E-4	6.918042795681391E-4	2.700351277124707E-4
95-99	0.004693467695954848	4.757761773981627E-4	6.943760426892105E-4	3.471880213446052E-4	6.943760426892104E-4	2.700351277124707E-4
100-104	0.00469861122219699	4.860632298824473E-4	7.072348582945661E-4	3.8319270503960127E-4	6.995195689313528E-4	2.700351277124707E-4
105-109	0.004706326511560204	5.297832029406568E-4	7.56098357594918E-4	4.0376681000817047E-4	7.149501476577796E-4	2.700351277124707E-4
110-114	0.004719185327165559	5.426420185460126E-4	7.895312781688429E-4	4.1148209937138397E-4	7.355242526263489E-4	2.700351277124707E-4
115-119	0.004755190010860557	5.632161235145817E-4	8.306794881059815E-4	4.1148209937138397E-4	7.792442256845583E-4	2.700351277124707E-4
120-124	0.0048169123257662625	5.837902284831508E-4	8.58968882437764E-4	4.1148209937138397E-4	7.946748044109851E-4	2.700351277124707E-4
125-129	0.004909495798124825	6.172231490570759E-4	8.641124086799063E-4	4.243409149767397E-4	8.306794881059815E-4	2.700351277124707E-4
130-134	0.004989220454878031	6.172231490570759E-4	8.949735661327602E-4	4.423432568242378E-4	8.435383037113372E-4	2.700351277124707E-4
135-139	0.005045799243541595	6.300819646624316E-4	9.001170923749024E-4	4.7577617739816264E-4	8.666841718009775E-4	2.7260689083354186E-4
140-144	0.005153813294626584	6.429407802677874E-4	9.181194342224005E-4	5.014938086088741E-4	8.846865136484755E-4	3.0346804828639565E-4
145-149	0.0052361097145008605	6.53227832752072E-4	9.515523547963253E-4	5.297832029406568E-4	8.949735661327602E-4	3.0861157452853795E-4
150-154	0.005338980239343707	6.763737008417123E-4	9.695546966438234E-4	5.477855447881549E-4	9.232629604645426E-4	3.0861157452853795E-4
155-159	0.005395559028007271	6.840889902049258E-4	9.875570384913215E-4	5.632161235145818E-4	9.438370654331118E-4	3.1889862701282254E-4
160-164	0.00545985310603405	6.943760426892104E-4	0.0010158464328231041	5.966490440885068E-4	9.875570384913215E-4	3.214703901338937E-4
165-166	0.005529290710302972	6.943760426892104E-4	0.0010158464328231041	6.043643334517202E-4	0.0010029876172177484	3.214703901338937E-4
>>END_MODULE
