##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479528
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6784176
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.300072108978306	39.0	39.0	39.0	37.0	39.0
2	40.083969814462364	41.0	40.0	41.0	38.0	41.0
3	40.1339315194653	41.0	40.0	41.0	38.0	41.0
4	40.15902432366141	41.0	40.0	41.0	38.0	41.0
5	40.171865382030184	41.0	40.0	41.0	38.0	41.0
6	40.160373492668825	41.0	40.0	41.0	38.0	41.0
7	40.186831090466995	41.0	40.0	41.0	38.0	41.0
8	40.160535487286886	41.0	40.0	41.0	38.0	41.0
9	40.134719087476505	41.0	40.0	41.0	38.0	41.0
10-14	40.073515250783586	41.0	40.0	41.0	38.0	41.0
15-19	39.94425451226501	41.0	40.0	41.0	38.0	41.0
20-24	39.758771175747796	41.0	39.8	41.0	37.6	41.0
25-29	39.69043942845822	40.6	39.6	41.0	37.0	41.0
30-34	39.599555966708415	41.0	39.0	41.0	36.6	41.0
35-39	39.290875413609555	40.0	38.6	41.0	35.4	41.0
40-44	38.82796949843282	40.0	37.6	41.0	35.0	41.0
45-49	38.39821304753886	39.6	36.4	41.0	35.0	41.0
50-54	37.80538137571902	38.6	35.2	40.8	34.8	41.0
55-59	37.07386836662256	36.8	35.0	39.6	34.0	41.0
60-64	36.250893933176265	35.8	34.8	38.4	33.6	40.4
65-69	35.78677375115268	35.0	35.0	37.0	33.8	39.0
70-74	35.46602393569978	35.0	35.0	36.0	34.0	38.8
75-79	35.221849639514076	35.0	35.0	35.8	33.2	37.4
80-84	35.09283423661178	35.0	35.0	35.0	33.0	37.0
85-89	34.84393529884837	35.0	34.6	35.0	32.4	37.8
90-94	39.548783902719705	40.6	39.8	40.6	37.6	40.6
95-99	39.83286893866362	41.0	40.0	41.0	37.8	41.0
100-104	39.72211310147175	41.0	39.8	41.0	37.0	41.0
105-109	39.56864573122478	41.0	39.0	41.0	36.4	41.0
110-114	39.37609370525191	40.2	39.0	41.0	35.8	41.0
115-119	39.141329806228455	40.0	38.0	41.0	35.0	41.0
120-124	39.15379909245452	40.0	38.0	41.0	35.0	41.0
125-129	38.68401398237793	39.8	37.6	40.8	34.8	41.0
130-134	38.436430548956096	39.8	36.4	41.0	34.6	41.0
135-139	37.939382471744366	39.0	35.2	41.0	34.0	41.0
140-144	37.293397153993446	37.4	35.0	40.2	34.0	41.0
145-149	36.61367338005526	36.2	35.0	39.2	33.4	41.0
150-154	36.12073193410071	35.4	35.0	37.8	34.0	40.2
155-159	35.53216300977771	35.0	35.0	36.4	33.8	38.8
160-164	35.07271019099306	35.0	35.0	35.8	33.0	37.0
165-169	34.77176108163577	35.0	35.0	35.0	33.0	36.0
170-174	34.59942469995367	35.0	35.0	35.0	33.0	36.0
175-178	34.20955593825595	34.75	34.5	35.0	32.0	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	13.0
30	202.0
31	1868.0
32	10522.0
33	43764.0
34	136548.0
35	353783.0
36	885037.0
37	1970034.0
38	2512607.0
39	849252.0
40	20544.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.958123727922153	28.273706342524136	29.25761359964718	21.510556329906535
2	21.94144137770011	28.80604807422449	28.72998872670756	20.522521821367842
3	19.778776376084583	27.91944076922533	30.536162387296557	21.765620467393536
4	20.45826936093639	27.011887663291756	31.02308961324117	21.506753362530688
5	21.435823598916066	27.743575638367872	29.05555516248399	21.76504560023207
6	21.257202053720302	28.087390421474918	29.078019202332015	21.577388322472764
7	21.272782427814374	28.174121661938017	28.883669881205915	21.6694260290417
8	21.49675951803137	28.302140156741217	28.484490968394688	21.716609356832723
9	21.512192490289166	28.466050998676923	28.531335271962284	21.490421239071626
10-14	21.601120018112738	28.248580225513013	28.36765732492789	21.78264243144635
15-19	21.730880802620685	28.07611712903675	28.3363049543526	21.856697113989966
20-24	21.78434639667367	27.943903577973213	28.27142751013535	22.000322515217764
25-29	21.907067269481217	27.883079684253474	28.063605661173884	22.146247385091424
30-34	21.941721441189028	27.943019166955573	28.045463443165392	22.06979594869001
35-39	21.92958142595357	27.94036003782921	28.007333536158256	22.12272500005896
40-44	21.981611326121257	27.89446500208721	27.91323515191823	22.210688519873305
45-49	22.137285353446018	27.850465553959687	27.861178719420014	22.15107037317428
50-54	22.13665447358677	27.893079424826244	27.8409846678506	22.129281433736388
55-59	22.069701611514795	27.817191063439388	27.886039513125837	22.227067811919973
60-64	22.06856661737549	27.86731947991915	27.9200628049744	22.144051097730955
65-69	22.0529832952447	27.865420944267953	27.891089500036554	22.19050626045079
70-74	22.016825624806906	27.842016480704512	27.9170410673308	22.224116827157786
75-79	21.978409758237405	27.8645807538012	27.93901278504567	22.217996702915727
80-84	21.98691779222709	27.870096530514537	27.9120972097422	22.23088846751617
85-89	21.96550914952678	27.827382426399318	27.877274999941037	22.329833424132865
90-94	20.976114466099773	28.028363537077023	29.468268451381068	21.527253545442136
95-99	21.358359378270006	28.314174284150184	28.60701931927122	21.720447018308587
100-104	21.604989707319184	28.225538159471135	28.325481860534758	21.843990272674922
105-109	21.682226438737516	28.056787679864435	28.282999176474217	21.97798670492383
110-114	21.75689885091655	27.950731209207007	28.210062104233423	22.082307835643018
115-119	21.88994619392614	27.979164387791517	27.99068909177498	22.140200326507365
120-124	21.90221330961803	27.96181291792606	27.96330934758912	22.17266442486679
125-129	21.899069438179232	27.970795725682013	27.982159221275747	22.147975614863004
130-134	21.99637057032188	27.97432771529904	27.861821293625006	22.16748042075407
135-139	22.060923520586538	27.92120792193832	27.8182106251399	22.199657932335246
140-144	22.0468065413926	28.008362323893	27.83205193201853	22.11277920269587
145-149	21.98987910520415	28.05844449738286	27.84629298445563	22.105383412957362
150-154	21.95380820173177	28.05148015809103	27.909058391386537	22.085653248790656
155-159	21.89992734545306	28.098035842109688	27.993226486694816	22.008810325742438
160-164	21.89216806410484	28.182352154711733	27.957250783336708	21.968228997846722
165-169	21.812268472432397	28.197202841718955	27.997752106201656	21.992776579646993
170-174	21.77034863281495	28.279983840874678	28.06490942979758	21.884758096512794
175-178	21.770864782947786	28.353631169365855	27.94181403432286	21.9336900133635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	3.0
8	5.5
9	10.0
10	32.0
11	63.5
12	129.5
13	246.0
14	434.0
15	708.5
16	1157.5
17	1947.5
18	3068.0
19	4702.5
20	7139.5
21	10503.5
22	15034.5
23	20701.0
24	27659.0
25	36047.0
26	45968.0
27	57148.5
28	69205.0
29	82229.0
30	96486.5
31	111333.5
32	126911.5
33	143532.0
34	161247.5
35	179514.0
36	198022.5
37	216936.5
38	235360.0
39	253261.0
40	269351.5
41	282030.0
42	290842.0
43	295743.5
44	296838.5
45	293113.0
46	284750.5
47	273246.0
48	258433.0
49	241582.0
50	222921.5
51	202785.5
52	183384.5
53	165208.5
54	147862.5
55	131539.0
56	116750.5
57	103217.0
58	91246.0
59	81044.5
60	71745.0
61	63370.0
62	55656.5
63	48415.5
64	41947.5
65	35770.0
66	29918.5
67	24691.5
68	19780.0
69	15396.0
70	11817.5
71	8845.0
72	6370.0
73	4405.5
74	3037.5
75	2011.5
76	1266.5
77	774.5
78	465.0
79	247.0
80	128.5
81	75.0
82	40.5
83	22.0
84	9.0
85	7.0
86	2.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	945.0
92-93	2133.0
94-95	3205.0
96-97	4350.0
98-99	5444.0
100-101	6534.0
102-103	7268.0
104-105	7990.0
106-107	8865.0
108-109	9961.0
110-111	10361.0
112-113	11158.0
114-115	11377.0
116-117	12521.0
118-119	12813.0
120-121	13384.0
122-123	13692.0
124-125	14833.0
126-127	15453.0
128-129	16784.0
130-131	16559.0
132-133	18469.0
134-135	78949.0
136-137	81208.0
138-139	76258.0
140-141	79386.0
142-143	81407.0
144-145	85169.0
146-147	82298.0
148-149	72236.0
150-151	66750.0
152-153	79635.0
154-155	74343.0
156-157	74040.0
158-159	77045.0
160-161	75723.0
162-163	68888.0
164-165	71587.0
166-167	75619.0
168-169	78147.0
170-171	79729.0
172-173	93305.0
174-175	93079.0
176-177	283707.0
178-179	4631569.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.54975261276095
#Duplication Level	Percentage of total
1	86.80322724222968
2	9.837161437337617
3	1.4661125343327106
4	0.5573084608516182
5	0.3753116617935124
6	0.28899583424628955
7	0.18364546849857713
8	0.15661233770247995
9	0.10693631634220535
>10	0.22129678547688453
>50	0.0014326650235277707
>100	0.001959256164937688
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.2110275440967333E-4	0.0	0.0	2.948036725462311E-5	2.948036725462311E-5	0.0
2	5.30646610583216E-4	0.0	0.0	2.948036725462311E-5	2.948036725462311E-5	0.0
3	5.30646610583216E-4	0.0	0.0	2.948036725462311E-5	2.948036725462311E-5	0.0
4	5.453867942105276E-4	0.0	0.0	5.896073450924622E-5	2.948036725462311E-5	0.0
5	5.453867942105276E-4	1.4740183627311556E-5	0.0	5.896073450924622E-5	4.422055088193467E-5	0.0
6	5.453867942105276E-4	1.4740183627311556E-5	0.0	5.896073450924622E-5	4.422055088193467E-5	0.0
7	5.453867942105276E-4	2.948036725462311E-5	1.4740183627311556E-5	5.896073450924622E-5	4.422055088193467E-5	0.0
8	5.453867942105276E-4	4.422055088193467E-5	1.4740183627311556E-5	5.896073450924622E-5	4.422055088193467E-5	0.0
9	5.453867942105276E-4	4.422055088193467E-5	1.4740183627311556E-5	7.370091813655778E-5	4.422055088193467E-5	0.0
10-14	5.453867942105276E-4	4.422055088193467E-5	2.65323305291608E-5	1.061293221166432E-4	5.30646610583216E-5	0.0
15-19	5.512828676614522E-4	5.30646610583216E-5	3.8324477431010045E-5	1.6803809335135173E-4	5.896073450924622E-5	0.0
20-24	5.630750145633014E-4	7.664895486202009E-5	7.95969915874824E-5	1.827782769786633E-4	5.896073450924622E-5	0.0
25-29	6.013994919943115E-4	1.1202539556756782E-4	1.2676557919487938E-4	1.9162238715505022E-4	5.896073450924622E-5	0.0
30-34	6.751004101308692E-4	1.4445379954765324E-4	1.32661652645804E-4	1.9162238715505022E-4	5.896073450924622E-5	0.0
35-39	7.871258056984371E-4	1.6803809335135173E-4	1.4740183627311556E-4	2.1815471768421102E-4	7.664895486202009E-5	1.4740183627311556E-5
40-44	8.107100995021356E-4	1.9162238715505022E-4	1.562459464495025E-4	2.5058312166429644E-4	1.2381754246941707E-4	5.30646610583216E-5
45-49	8.313463565803716E-4	1.9162238715505022E-4	1.9162238715505022E-4	2.712193787425326E-4	1.7688220352773867E-4	9.728521194025627E-5
50-54	8.81462980913231E-4	2.0046649733143716E-4	2.2405079113513564E-4	2.918556358207688E-4	2.0046649733143716E-4	1.1792146901849244E-4
55-59	9.433717521479396E-4	2.0636257078236178E-4	2.4763508493883413E-4	3.50816370330015E-4	2.4763508493883413E-4	1.4445379954765324E-4
60-64	9.66956045951638E-4	2.0636257078236178E-4	2.8301152564438187E-4	3.773487008591758E-4	2.859595623698442E-4	1.7393416680227636E-4
65-69	0.001005280523382648	2.2110275440967333E-4	2.948036725462311E-4	3.8914084776102507E-4	3.2723207652631653E-4	1.9457042388051253E-4
70-74	0.001279447938850643	2.2110275440967333E-4	3.478683336045527E-4	4.009329946628743E-4	3.50816370330015E-4	2.5058312166429644E-4
75-79	0.006839445203072561	2.5353115838975876E-4	3.8619281103556276E-4	4.392574720938843E-4	4.186212150156482E-4	2.8006348891891956E-4
80-84	0.009256835317951656	2.65323305291608E-4	3.9798495793741205E-4	4.716858760739698E-4	6.16139675621623E-4	2.8006348891891956E-4
85-89	0.009327588199362752	3.12491892899005E-4	4.2156925174111054E-4	5.011662433285929E-4	9.581119357752512E-4	2.8006348891891956E-4
90-94	0.009442561631655782	3.242840398008542E-4	4.4220550881934667E-4	5.218025004068291E-4	9.905403397553365E-4	2.8006348891891956E-4
95-99	0.009480886109086793	3.3607618670270347E-4	4.4220550881934667E-4	5.424387574850652E-4	0.0010023324866571858	2.8006348891891956E-4
100-104	0.009522158623243265	3.744006641337135E-4	4.4220550881934667E-4	5.68971088014226E-4	0.0010023324866571858	2.8006348891891956E-4
105-109	0.009536898806870576	3.9798495793741205E-4	4.6578980262304516E-4	6.16139675621623E-4	0.0010141246335590348	2.8006348891891956E-4
110-114	0.009569327210850662	3.9798495793741205E-4	4.864260597012813E-4	6.603602265035577E-4	0.001040656964088196	2.8006348891891956E-4
115-119	0.009592911504654359	4.1272514156472356E-4	4.864260597012813E-4	7.340611446401155E-4	0.001052449110990045	2.8006348891891956E-4
120-124	0.009645976165712682	4.481015822702713E-4	4.864260597012813E-4	7.871258056984371E-4	0.0010671892946173566	2.8301152564438187E-4
125-129	0.009737365304202014	4.716858760739698E-4	4.982182066031306E-4	8.34294393305834E-4	0.001081929478244668	2.948036725462311E-4
130-134	0.009875923030298741	4.716858760739698E-4	5.011662433285929E-4	9.256835317951658E-4	0.0011025657353229044	2.948036725462311E-4
135-139	0.009993844499317233	4.864260597012813E-4	5.30646610583216E-4	9.37475678697015E-4	0.0011232019924011405	3.0364778272261805E-4
140-144	0.010117662041786652	5.247505371322914E-4	5.542309043869145E-4	9.993844499317235E-4	0.0011349941393029898	3.154399296244673E-4
145-149	0.010265063878059767	5.335946473086783E-4	5.601269778378391E-4	0.001022968743735422	0.0011497343229303013	3.3312814997724116E-4
150-154	0.010326972649294476	5.512828676614522E-4	5.630750145633014E-4	0.0010642412578918943	0.0011497343229303013	3.390242234281658E-4
155-159	0.010374141236901873	5.955034185433868E-4	5.748671614651507E-4	0.0010996176985974423	0.0011644745065576129	3.390242234281658E-4
160-164	0.010465530375391205	6.338278959743969E-4	5.896073450924622E-4	0.001137942176028452	0.001193954873812236	3.478683336045527E-4
165-167	0.010711200102513063	6.387412905168341E-4	5.896073450924622E-4	0.0011743012956424873	0.0012234352410668591	3.5376440705547733E-4
>>END_MODULE
