##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479530
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6801081
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.2994396037924	39.0	39.0	39.0	37.0	39.0
2	40.0910368513476	41.0	40.0	41.0	38.0	41.0
3	40.1363261222738	41.0	40.0	41.0	38.0	41.0
4	40.1615809310314	41.0	40.0	41.0	38.0	41.0
5	40.176817920562925	41.0	40.0	41.0	38.0	41.0
6	40.17015647953612	41.0	40.0	41.0	38.0	41.0
7	40.19233971775957	41.0	40.0	41.0	38.0	41.0
8	40.16270619332427	41.0	40.0	41.0	38.0	41.0
9	40.13715349074654	41.0	40.0	41.0	38.0	41.0
10-14	40.076299400051255	41.0	40.0	41.0	38.0	41.0
15-19	39.94435311092458	41.0	40.0	41.0	38.0	41.0
20-24	39.76620351970517	41.0	39.8	41.0	37.6	41.0
25-29	39.68965418879734	40.6	39.6	41.0	37.0	41.0
30-34	39.607337333579764	41.0	39.0	41.0	36.6	41.0
35-39	39.29936002820729	40.2	38.6	41.0	35.2	41.0
40-44	38.828880320643144	40.0	37.4	41.0	35.0	41.0
45-49	38.394185159682706	39.4	36.4	41.0	35.0	41.0
50-54	37.81624638789039	38.6	35.2	40.8	34.8	41.0
55-59	37.090229273846326	36.8	35.0	39.6	34.0	41.0
60-64	36.27516799167662	35.8	34.8	38.6	33.6	40.6
65-69	35.81552320873696	35.0	35.0	37.0	33.8	39.4
70-74	35.48423313882014	35.0	35.0	36.2	34.0	39.0
75-79	35.23657662656863	35.0	35.0	36.0	33.2	37.4
80-84	35.1207416291616	35.0	35.0	35.0	33.0	37.0
85-89	34.87172115726897	35.0	34.6	35.0	32.4	38.0
90-94	39.56234500742966	40.6	39.8	40.6	37.6	40.6
95-99	39.83140574659137	41.0	40.0	41.0	37.6	41.0
100-104	39.718563255767585	41.0	39.8	41.0	37.0	41.0
105-109	39.567296531293636	41.0	39.0	41.0	36.4	41.0
110-114	39.37695570155644	40.2	39.0	41.0	35.8	41.0
115-119	39.133013532151146	40.0	38.0	41.0	35.0	41.0
120-124	39.147924858627604	40.0	38.0	41.0	35.0	41.0
125-129	38.68426089000766	39.8	37.6	40.8	34.8	41.0
130-134	38.4368624303299	40.0	36.4	41.0	34.8	41.0
135-139	37.94079419644356	39.0	35.2	41.0	34.0	41.0
140-144	37.305802564250186	37.4	35.0	40.2	34.0	41.0
145-149	36.62348785090508	36.2	35.0	39.2	33.4	41.0
150-154	36.122934982230504	35.4	35.0	37.8	34.0	40.2
155-159	35.53646016466659	35.0	35.0	36.4	34.0	38.8
160-164	35.07589070382391	35.0	35.0	35.8	33.0	37.0
165-169	34.77913079965035	35.0	35.0	35.0	33.0	36.0
170-174	34.60697877052269	35.0	35.0	35.0	33.0	36.0
175-178	34.215432750704046	34.75	34.5	35.0	32.0	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	15.0
30	223.0
31	1842.0
32	10460.0
33	43362.0
34	135849.0
35	350534.0
36	879667.0
37	1971986.0
38	2525890.0
39	860179.0
40	21074.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.984193542173664	28.236231857847304	29.26739146321004	21.512183136768993
2	21.943114631335813	28.752193952696636	28.711891536066105	20.592799879901445
3	19.813012078521048	27.886787409236856	30.5026068649969	21.797593647245193
4	20.463011688877106	26.961875619478725	30.996028425481185	21.579084266162983
5	21.477820952286848	27.66357289377968	29.039721773641574	21.818884380291898
6	21.303378095335137	28.017590144860794	29.031002571502974	21.648029188301095
7	21.308347893518693	28.11963274661778	28.834827875156904	21.73719148470662
8	21.53801726519652	28.27463751718293	28.438905521048785	21.74843969657177
9	21.544075125704282	28.452035786663917	28.508732655882202	21.4951564317496
10-14	21.627511861717277	28.210694741027197	28.332390100926602	21.829403296328923
15-19	21.758546913350983	28.061862518620202	28.299230666419056	21.880359901609758
20-24	21.8256862401727	27.894694975695778	28.240884059460548	22.038734724670974
25-29	21.952266117695114	27.83754817800288	28.046944301942588	22.163241402359418
30-34	21.978076720450765	27.913230264424136	27.993773342796537	22.11491967232856
35-39	21.980443991183165	27.91235099243782	27.961922523787024	22.145282492591985
40-44	22.010489214876284	27.86162670316675	27.89174544458447	22.2361386373725
45-49	22.163364912136764	27.824570829254935	27.841991589278237	22.170072669330068
50-54	22.178571318294843	27.851804735159014	27.7974045596575	22.172219386888642
55-59	22.126347267441748	27.775014001450653	27.847487774369988	22.25115095673761
60-64	22.098595796756427	27.83620427399703	27.883508518719303	22.181691410527236
65-69	22.105053593686065	27.809285023954278	27.8659583675007	22.21970301485896
70-74	22.038478882989338	27.817095547016716	27.87769179634826	22.26673377364569
75-79	22.021113996436743	27.82155072112801	27.914959401306938	22.24237588112831
80-84	22.030327237684716	27.831902016752924	27.869951850301444	22.267818895260916
85-89	21.987892807040527	27.803315384716047	27.838262770286075	22.370529037957347
90-94	21.030197202015444	27.978692577356	29.428318631547405	21.56279158908115
95-99	21.3965383056062	28.266252458504425	28.566686102431056	21.77052313345832
100-104	21.64770757711856	28.193534546677284	28.283264949321214	21.875492926882945
105-109	21.715262486948845	28.03788767383383	28.237128030189623	22.009721809027706
110-114	21.78628413423641	27.906591214011016	28.190755705349474	22.1163689464031
115-119	21.93697468481462	27.940975511295484	27.937963667915405	22.18408613597449
120-124	21.94052308476238	27.928794809717576	27.919721329888393	22.210960775631648
125-129	21.934839948855828	27.93014398165165	27.94482924691975	22.19018682257277
130-134	22.026120962227143	27.950213917931556	27.823982745118087	22.199682374723217
135-139	22.11406974138224	27.887714911514554	27.77200862326914	22.226206723834068
140-144	22.097422261827372	27.962523202140215	27.803617149242378	22.13643738679004
145-149	22.05261132130307	28.01076115652601	27.792833947998968	22.14379357417195
150-154	21.997881298781785	28.038671877086024	27.873764952467177	22.08968187166501
155-159	21.95126683530829	28.072525892340174	27.93765751503595	22.03854975731559
160-164	21.92798497461179	28.14372274237768	27.914332220885658	22.013960062124873
165-169	21.858106401063022	28.13356992517566	27.973359882870046	22.03496379089127
170-174	21.832440600995866	28.230418369678677	28.01089120828251	21.926249821042944
175-178	21.808066867831553	28.30188746995556	27.905379867999397	21.98466579421349
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	1.0
8	5.5
9	14.0
10	30.0
11	58.5
12	117.0
13	204.5
14	384.0
15	674.0
16	1110.5
17	1807.0
18	2908.5
19	4545.5
20	6998.5
21	10321.5
22	14549.0
23	20162.0
24	27158.0
25	35563.5
26	45388.5
27	56363.5
28	68498.0
29	81683.0
30	95493.0
31	110275.5
32	126164.5
33	142856.5
34	160581.0
35	178705.0
36	197252.5
37	216306.0
38	235069.0
39	253341.0
40	268934.0
41	281740.0
42	291312.0
43	296210.5
44	297294.5
45	294275.0
46	286557.5
47	274404.5
48	260046.5
49	243429.5
50	224170.0
51	204590.5
52	185502.5
53	166836.0
54	149079.0
55	132573.0
56	117649.5
57	104355.0
58	92456.0
59	82049.5
60	72792.0
61	64406.5
62	56469.0
63	49105.0
64	42712.5
65	36430.5
66	30403.5
67	24906.0
68	19957.0
69	15840.0
70	12138.5
71	9034.5
72	6585.5
73	4587.5
74	3139.0
75	2080.5
76	1332.5
77	835.0
78	506.0
79	270.0
80	152.5
81	84.5
82	40.0
83	19.0
84	11.0
85	6.0
86	3.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	927.0
92-93	2273.0
94-95	4550.0
96-97	4830.0
98-99	5619.0
100-101	6956.0
102-103	7403.0
104-105	8695.0
106-107	9653.0
108-109	9718.0
110-111	10691.0
112-113	11622.0
114-115	11584.0
116-117	12461.0
118-119	13180.0
120-121	12951.0
122-123	13623.0
124-125	15073.0
126-127	15961.0
128-129	16614.0
130-131	17283.0
132-133	18797.0
134-135	76550.0
136-137	86963.0
138-139	78075.0
140-141	82382.0
142-143	77371.0
144-145	91152.0
146-147	90916.0
148-149	70381.0
150-151	75260.0
152-153	69261.0
154-155	69110.0
156-157	68428.0
158-159	64912.0
160-161	68479.0
162-163	70343.0
164-165	73836.0
166-167	73411.0
168-169	78696.0
170-171	81049.0
172-173	86976.0
174-175	91827.0
176-177	280584.0
178-179	4664655.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.54687816450794
#Duplication Level	Percentage of total
1	86.7793865381026
2	9.847174393916728
3	1.5278849123293956
4	0.5674934252089181
5	0.37426239194299127
6	0.2817244197840305
7	0.19510571992348216
8	0.12611724379301156
9	0.09210205745676482
>10	0.204977792733131
>50	0.0015449851327616537
>100	0.002226119676176633
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.0292481445229074E-4	0.0	0.0	2.9407089843511643E-5	1.4703544921755822E-5	0.0
2	6.322524316355003E-4	0.0	0.0	2.9407089843511643E-5	2.9407089843511643E-5	0.0
3	6.322524316355003E-4	0.0	0.0	2.9407089843511643E-5	2.9407089843511643E-5	0.0
4	6.322524316355003E-4	0.0	0.0	4.4110634765267465E-5	2.9407089843511643E-5	0.0
5	6.322524316355003E-4	0.0	0.0	7.35177246087791E-5	2.9407089843511643E-5	0.0
6	6.322524316355003E-4	0.0	0.0	8.822126953053493E-5	2.9407089843511643E-5	0.0
7	6.61659521479012E-4	0.0	2.9407089843511643E-5	8.822126953053493E-5	2.9407089843511643E-5	0.0
8	6.61659521479012E-4	0.0	5.8814179687023286E-5	1.0292481445229074E-4	2.9407089843511643E-5	0.0
9	6.61659521479012E-4	0.0	5.8814179687023286E-5	1.0292481445229074E-4	2.9407089843511643E-5	0.0
10-14	6.793037753851189E-4	1.7644253906106985E-5	5.881417968702329E-5	1.1762835937404656E-4	4.1169925780916295E-5	0.0
15-19	6.969480292912259E-4	7.057701562442794E-5	5.881417968702329E-5	1.5879828515496285E-4	7.939914257748143E-5	0.0
20-24	7.116515742129818E-4	8.822126953053493E-5	8.822126953053494E-5	1.7938324804542104E-4	8.822126953053493E-5	0.0
25-29	7.557622089782492E-4	1.0292481445229074E-4	1.0292481445229074E-4	2.0879033788893264E-4	9.410268749923726E-5	0.0
30-34	8.05754261712219E-4	1.235097773427489E-4	1.2056906835839774E-4	2.9113018945076526E-4	1.1174694140534425E-4	2.9407089843511642E-6
35-39	8.469241874931352E-4	1.7350183007671868E-4	1.470354492175582E-4	3.2935940624733036E-4	1.5879828515496285E-4	2.3525671874809314E-5
40-44	8.704498593679446E-4	1.9114608398282566E-4	1.5291686718626056E-4	3.6464791405954437E-4	2.2643459179503964E-4	2.9407089843511647E-5
45-49	9.175012031175633E-4	2.0879033788893264E-4	1.6762041210801636E-4	3.675886230438955E-4	3.1465586132557456E-4	2.9407089843511647E-5
50-54	0.0010292481445229073	2.234938828106885E-4	2.058496289045815E-4	3.675886230438955E-4	3.6464791405954437E-4	4.705134374961863E-5
55-59	0.0010968844511629842	2.646638085916048E-4	2.058496289045815E-4	3.999364218717583E-4	3.8523287695000247E-4	6.469559765572561E-5
60-64	0.0011233508320221447	2.7054522656030716E-4	2.058496289045815E-4	4.6463201952748394E-4	4.175806757778653E-4	1.058655234366419E-4
65-69	0.0011468765038969541	2.9701160741946755E-4	2.234938828106885E-4	4.940391093709956E-4	4.616913105431328E-4	1.235097773427489E-4
70-74	0.0015497536347530635	3.117151523412234E-4	2.5878239062290245E-4	5.793196699171794E-4	5.087426542927513E-4	1.5291686718626056E-4
75-79	0.007672309740172188	3.4700366015343737E-4	2.940708984351164E-4	5.940232148389351E-4	5.499125800736678E-4	1.7350183007671868E-4
80-84	0.010098394652261899	3.528850781221397E-4	3.323001152316815E-4	6.028453417919887E-4	6.763630664007676E-4	1.7644253906106986E-4
85-89	0.01010721677921495	3.528850781221397E-4	3.675886230438955E-4	6.146081777293933E-4	9.939596367106935E-4	1.7644253906106986E-4
90-94	0.01025425222843251	3.528850781221397E-4	3.76410749996949E-4	6.734223574164166E-4	0.0010321888535072586	1.7644253906106986E-4
95-99	0.010292481445229073	3.558257871064909E-4	4.0287713085610953E-4	7.381179550721422E-4	0.0010439516894446632	1.7644253906106986E-4
100-104	0.010316007117103884	3.675886230438955E-4	4.352249296839723E-4	7.939914257748144E-4	0.0010704180703038238	1.7644253906106986E-4
105-109	0.010357177042884799	3.999364218717583E-4	4.734541464805375E-4	8.204578066339748E-4	0.001073358779288175	1.7644253906106986E-4
110-114	0.010365999169837853	4.234620937465676E-4	4.999205273396979E-4	8.763312773366469E-4	0.001073358779288175	1.7644253906106986E-4
115-119	0.010368939878822205	4.411063476526747E-4	5.058019453084002E-4	8.969162402271051E-4	0.001073358779288175	1.7644253906106986E-4
120-124	0.010454220439368389	4.411063476526747E-4	5.646161249954235E-4	9.057383671801586E-4	0.0011057065781160378	1.8526466601412333E-4
125-129	0.010598315179601595	4.5875060155878164E-4	6.116674687450421E-4	9.233826210862656E-4	0.0011409950859282518	1.9114608398282566E-4
130-134	0.010695358576085185	5.058019453084002E-4	6.293117226511491E-4	9.380861660080213E-4	0.0011733428847561145	1.9702750195152801E-4
135-139	0.010774757718662663	5.146240722614537E-4	6.675409394477143E-4	9.498490019454259E-4	0.0011939278476465727	2.058496289045815E-4
140-144	0.010868860406161904	5.293276171832096E-4	6.940073203068747E-4	9.763153828045865E-4	0.0012321570644431377	2.058496289045815E-4
145-149	0.010945318839755034	5.352090351519119E-4	7.17532992181684E-4	0.001038070271475961	0.0012498013183492448	2.058496289045815E-4
150-154	0.011001192310457705	5.793196699171794E-4	7.52821499993898E-4	0.001076299488272526	0.0012645048632710005	2.1761246484198614E-4
155-159	0.011083532162019539	5.881417968702328E-4	7.763471718687073E-4	0.0011380543769439005	0.0012645048632710005	2.2055317382633734E-4
160-164	0.011207041939362287	6.057860507763398E-4	7.822285898374098E-4	0.0011586393398343587	0.0012733269902240542	2.2055317382633734E-4
165-167	0.011326630771392567	6.175488867137445E-4	7.939914257748144E-4	0.0011909871386622216	0.0012939119531145122	2.3525671874809315E-4
>>END_MODULE
