##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479538
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5330336
Total Bases	896.7 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.273789869906885	39.0	39.0	39.0	37.0	39.0
2	40.01404920815499	41.0	40.0	41.0	38.0	41.0
3	40.06496119569198	41.0	40.0	41.0	38.0	41.0
4	40.08414854148031	41.0	40.0	41.0	38.0	41.0
5	40.091626306484244	41.0	40.0	41.0	38.0	41.0
6	40.082369291541845	41.0	40.0	41.0	38.0	41.0
7	40.1043926686798	41.0	40.0	41.0	38.0	41.0
8	40.07269954464409	41.0	40.0	41.0	38.0	41.0
9	40.03474621487276	41.0	40.0	41.0	38.0	41.0
10-14	39.98262987549003	41.0	40.0	41.0	38.0	41.0
15-19	39.8311070446591	41.0	40.0	41.0	37.8	41.0
20-24	39.61169228356336	40.6	39.2	41.0	36.8	41.0
25-29	39.39635291283702	40.0	39.0	41.0	35.8	41.0
30-34	39.06974738553067	40.0	38.0	41.0	35.0	41.0
35-39	38.489613112569266	39.8	36.6	41.0	35.0	41.0
40-44	37.74881257016443	38.6	35.0	40.8	34.0	41.0
45-49	37.18086814789912	37.0	35.0	40.0	34.0	41.0
50-54	36.60054525643412	35.6	35.0	39.2	34.0	41.0
55-59	36.04315288942386	35.0	35.0	37.8	33.0	40.6
60-64	35.46916907301904	35.0	34.8	36.6	32.6	39.4
65-69	35.2635734782948	35.0	34.8	36.0	33.0	39.0
70-74	35.154370793886166	35.0	35.0	35.4	33.0	37.8
75-79	35.03019014185973	35.0	35.0	35.0	33.0	37.0
80-84	34.98214090068618	35.0	35.0	35.0	33.0	37.4
85-89	34.77654444297695	35.0	34.6	35.0	32.2	38.2
90-94	39.363263407413015	40.6	39.0	40.6	36.6	40.6
95-99	39.58095981335751	41.0	39.0	41.0	36.2	41.0
100-104	39.44000671921882	41.0	39.0	41.0	35.8	41.0
105-109	39.252156432466606	40.2	38.6	41.0	35.0	41.0
110-114	39.006178156365365	40.0	38.0	41.0	35.0	41.0
115-119	38.67132995528648	40.0	37.6	41.0	34.6	41.0
120-124	38.431238118067576	40.0	36.6	41.0	34.0	41.0
125-129	37.74620808858785	38.6	35.2	40.6	33.6	41.0
130-134	37.29813850136178	37.6	35.0	40.0	33.0	41.0
135-139	36.695119489079026	36.4	35.0	39.8	33.0	41.0
140-144	36.09138093166422	35.0	35.0	38.8	33.0	41.0
145-149	35.58210535977632	35.0	35.0	37.0	33.0	39.8
150-154	35.34764443025885	35.0	35.0	36.0	33.0	39.0
155-159	34.97767348668569	35.0	35.0	35.4	33.0	37.0
160-164	34.695115734358446	35.0	35.0	35.0	33.0	36.2
165-169	34.504463347364556	35.0	34.2	35.0	33.0	36.0
170-174	34.39021975167527	35.0	34.0	35.0	33.0	35.4
175-178	34.030232074118715	34.75	33.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	32.0
30	315.0
31	2536.0
32	14456.0
33	58664.0
34	182942.0
35	484181.0
36	1219678.0
37	1854024.0
38	1160784.0
39	348049.0
40	4674.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.595191747762243	26.20343257910946	26.32999120505724	23.871384468071057
2	24.05885857852113	26.417434097963056	26.621192360106377	22.902514963409438
3	22.412358245333877	25.9633163838077	27.755754984301177	23.868570386557245
4	22.89593001266712	24.993658936322213	28.048494503911197	24.06191654709947
5	23.700457156922187	25.605796707749757	26.738445756515162	23.955300378812893
6	23.496004754672125	25.983258841468903	26.802250364704967	23.718486039154005
7	23.59267783494324	25.914276323293688	26.42932828249476	24.06371755926831
8	23.84410663793052	26.075448151861348	26.180751082108145	23.89969412809999
9	23.703252477892576	26.263353754810204	26.277499204552957	23.755894562744263
10-14	23.852327508059528	26.033143126437057	26.081582849561453	24.03294651594196
15-19	23.932705180311334	25.875592082750504	26.070570410570742	24.121132326367416
20-24	24.014392338494233	25.794081273675808	25.97112076987267	24.22040561795729
25-29	24.11756782311659	25.731105881505407	25.818012222869253	24.33331407250875
30-34	24.11419842951739	25.7676251553373	25.773744844602664	24.344431570542646
35-39	24.125424738703153	25.77387992051533	25.761790626331997	24.338904714449523
40-44	24.21045877783314	25.690290443229095	25.62198705672588	24.477263722211884
45-49	24.413327790218105	25.61811863267156	25.56341288804308	24.405140689067256
50-54	24.436924801738577	25.64573040048507	25.554843071806353	24.362501725969995
55-59	24.374827403000488	25.60371803953822	25.55975458207513	24.461699975386168
60-64	24.307435778907745	25.651399836708226	25.625172596999512	24.41599178738451
65-69	24.319168622765993	25.6644346622802	25.609379971544005	24.407016743409795
70-74	24.271588132530482	25.652296590683964	25.60745513978856	24.46866013699699
75-79	24.24068201329147	25.643212735557384	25.650075342342397	24.46602990880875
80-84	24.245728599472905	25.68544271880797	25.60619067916169	24.462638002557437
85-89	24.24858395418225	25.62720623990683	25.556302642084848	24.56790716382607
90-94	23.362820074183244	25.90929419329288	26.888231820909336	23.83965391161454
95-99	23.61056571895868	26.062138842945632	26.30520643565998	24.022089002435713
100-104	23.8136707479993	26.023030823212395	26.06683951580271	24.096458912985597
105-109	23.870884348078583	25.855080029581828	26.01805882120579	24.255976801133798
110-114	23.926905499356092	25.803621406447892	25.94382981704861	24.32564327714741
115-119	24.056130040350737	25.812923645473358	25.785035174377274	24.345911139798634
120-124	24.05624662647224	25.80129977932127	25.716382088093155	24.426071506113338
125-129	24.035601534280673	25.816872225922342	25.737661340113466	24.409864899683516
130-134	24.195280272909308	25.79137363162305	25.59286345843769	24.420482637029952
135-139	24.305595090279212	25.754774096752296	25.50917274578561	24.430458067182887
140-144	24.228194634058045	25.85662338337874	25.559409002635842	24.355772979927373
145-149	24.182515525763918	25.94213788072605	25.59140765714112	24.283938936368912
150-154	24.08696138024286	25.971878089481987	25.69889828597116	24.24226224430399
155-159	24.0145469794665	26.04323946818396	25.79385105265765	24.148362499691896
160-164	23.979017201363526	26.149248844999494	25.827840904079384	24.04389304955759
165-169	23.878601280135197	26.1591223516838	25.889755257139825	24.072521111041176
170-174	23.79436238727838	26.298753211840655	25.95983741255593	23.947046988325038
175-178	23.787595983631054	26.378995965238982	25.87393239763662	23.959475653493346
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	1.0
10	3.0
11	5.0
12	20.5
13	41.0
14	74.5
15	141.0
16	234.5
17	423.0
18	767.5
19	1295.5
20	2171.5
21	3544.5
22	5375.5
23	7959.0
24	11318.0
25	15600.0
26	21068.0
27	27408.5
28	34716.5
29	42753.5
30	51292.0
31	60288.0
32	69478.0
33	78595.5
34	87976.0
35	97385.0
36	105559.0
37	113180.5
38	121073.5
39	128649.0
40	135853.0
41	142644.5
42	149274.5
43	155035.0
44	160610.0
45	167380.5
46	172893.0
47	177298.0
48	180965.5
49	183626.5
50	186023.0
51	187202.5
52	187021.0
53	185281.0
54	181829.0
55	177268.0
56	171235.5
57	163512.5
58	154812.0
59	145137.5
60	134058.5
61	121778.0
62	109612.0
63	97430.0
64	84422.5
65	71894.0
66	60283.5
67	49246.5
68	39357.5
69	30722.5
70	23532.5
71	17512.0
72	12585.5
73	8883.0
74	6064.0
75	4034.0
76	2562.0
77	1627.5
78	998.0
79	549.0
80	321.5
81	165.5
82	88.5
83	44.5
84	18.0
85	8.5
86	3.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1149.0
92-93	2477.0
94-95	3728.0
96-97	4991.0
98-99	6274.0
100-101	7434.0
102-103	8682.0
104-105	9568.0
106-107	10293.0
108-109	11224.0
110-111	12078.0
112-113	12514.0
114-115	12982.0
116-117	13624.0
118-119	14517.0
120-121	15324.0
122-123	15544.0
124-125	16985.0
126-127	16977.0
128-129	17571.0
130-131	18848.0
132-133	20196.0
134-135	78776.0
136-137	76210.0
138-139	75746.0
140-141	79188.0
142-143	78843.0
144-145	82197.0
146-147	86111.0
148-149	64660.0
150-151	63244.0
152-153	65045.0
154-155	63692.0
156-157	72260.0
158-159	67319.0
160-161	65836.0
162-163	64651.0
164-165	67524.0
166-167	72005.0
168-169	79255.0
170-171	74193.0
172-173	79432.0
174-175	81401.0
176-177	234281.0
178-179	3305487.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.39431375799961
#Duplication Level	Percentage of total
1	95.00981692436277
2	4.446322336125754
3	0.3758093817664104
4	0.09046785275858125
5	0.04047003484533722
6	0.019900635139210393
7	0.006343018216663505
8	0.004267986934308477
9	0.001076882909978823
>10	0.005524946940970816
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.7521086850810156E-5	0.0	0.0	1.8760543425405078E-5	0.0	0.0
2	9.380271712702539E-5	1.8760543425405078E-5	1.8760543425405078E-5	3.7521086850810156E-5	0.0	0.0
3	1.1256326055243046E-4	3.7521086850810156E-5	1.8760543425405078E-5	3.7521086850810156E-5	0.0	0.0
4	1.1256326055243046E-4	3.7521086850810156E-5	5.628163027621523E-5	3.7521086850810156E-5	0.0	0.0
5	1.1256326055243046E-4	5.628163027621523E-5	7.504217370162031E-5	3.7521086850810156E-5	0.0	0.0
6	1.1256326055243046E-4	5.628163027621523E-5	7.504217370162031E-5	3.7521086850810156E-5	0.0	0.0
7	1.1256326055243046E-4	5.628163027621523E-5	7.504217370162031E-5	5.628163027621523E-5	0.0	0.0
8	1.3132380397783554E-4	5.628163027621523E-5	7.504217370162031E-5	7.504217370162031E-5	0.0	0.0
9	1.5008434740324062E-4	5.628163027621523E-5	7.504217370162031E-5	7.504217370162031E-5	0.0	0.0
10-14	1.688448908286457E-4	5.628163027621523E-5	7.504217370162031E-5	1.1631536923751146E-4	1.5008434740324062E-5	0.0
15-19	1.688448908286457E-4	6.753795633145828E-5	7.504217370162031E-5	1.463322387181596E-4	2.6264760795567107E-5	0.0
20-24	1.7634910819880773E-4	9.380271712702539E-5	7.504217370162031E-5	1.650927821435647E-4	4.127319553589117E-5	0.0
25-29	2.2137441241977993E-4	9.380271712702539E-5	8.254639107178234E-5	2.0636597767945586E-4	5.628163027621523E-5	0.0
30-34	3.0767291217664324E-4	1.0881115186734945E-4	1.275716952927545E-4	2.3638284716010396E-4	9.755482581210641E-5	0.0
35-39	3.714587598230205E-4	1.3132380397783554E-4	1.5008434740324062E-4	2.9641658612140025E-4	1.3507591266291656E-4	0.0
40-44	3.827150858782636E-4	1.5383645608832165E-4	1.6134067345848367E-4	3.4144189034237236E-4	1.6134067345848367E-4	3.0016869480648123E-5
45-49	4.314924987843168E-4	1.913575429391318E-4	2.0636597767945586E-4	3.714587598230205E-4	2.3263073847502297E-4	3.7521086850810156E-5
50-54	4.314924987843168E-4	2.3263073847502297E-4	2.4388706453026598E-4	4.052277379887496E-4	3.9021930324842564E-4	3.7521086850810156E-5
55-59	5.14038889856099E-4	2.739039340109141E-4	2.7765604269599516E-4	4.314924987843168E-4	4.5025304220972184E-4	5.628163027621523E-5
60-64	5.965852809278815E-4	2.8140815138107616E-4	2.8140815138107616E-4	4.314924987843168E-4	4.8402202037545097E-4	5.628163027621523E-5
65-69	6.978922154250689E-4	2.8140815138107616E-4	2.8140815138107616E-4	4.6526147695004593E-4	5.1779099854118E-4	5.628163027621523E-5
70-74	0.0010168214536569553	2.926644774363192E-4	2.8140815138107616E-4	4.952783464306941E-4	6.003373896129625E-4	7.504217370162031E-5
75-79	0.004791442790848457	3.1892923823188633E-4	2.8891236875123815E-4	5.14038889856099E-4	6.528669112040968E-4	7.504217370162031E-5
80-84	0.006224748308549405	3.1892923823188633E-4	3.1517712954680534E-4	5.290473245964232E-4	8.029512586073372E-4	7.504217370162031E-5
85-89	0.006288534156195782	3.1892923823188633E-4	3.264334556020483E-4	5.440557593367472E-4	0.0010881115186734945	7.504217370162031E-5
90-94	0.006333559460416754	3.2268134691696733E-4	3.376897816572914E-4	5.478078680218282E-4	0.0010881115186734945	7.504217370162031E-5
95-99	0.006386088982007888	3.376897816572914E-4	3.639545424528585E-4	6.078416069831246E-4	0.0010881115186734945	7.504217370162031E-5
100-104	0.006404849525433294	3.5645032508269646E-4	3.7521086850810154E-4	6.416105851488537E-4	0.0010881115186734945	7.504217370162031E-5
105-109	0.00643111428622886	3.789629771931826E-4	3.827150858782636E-4	7.166527588504739E-4	0.0010881115186734945	7.504217370162031E-5
110-114	0.006446122720969184	3.9397141193350663E-4	4.127319553589117E-4	7.654301717565272E-4	0.0010993678447287374	7.504217370162031E-5
115-119	0.006453626938339346	4.239882814141547E-4	4.127319553589117E-4	7.879428238670133E-4	0.0011518973663198718	7.504217370162031E-5
120-124	0.006457379047024428	4.4650093352464085E-4	4.202361727290737E-4	8.142075846625805E-4	0.0011819142358005198	7.879428238670132E-5
125-129	0.006472387481764752	4.5025304220972184E-4	4.76517803005289E-4	8.667371062537146E-4	0.0011819142358005198	9.380271712702539E-5
130-134	0.006558685981521614	4.5025304220972184E-4	4.8777412906053196E-4	9.080103017896057E-4	0.0011819142358005198	9.380271712702539E-5
135-139	0.0066787534594442075	4.76517803005289E-4	4.915262377456131E-4	9.642919320658209E-4	0.0012231874313364112	9.380271712702539E-5
140-144	0.006716274546295017	5.06534672485937E-4	5.14038889856099E-4	0.0010018130189166311	0.0012757169529275453	1.1256326055243046E-4
145-149	0.006757547741830909	5.06534672485937E-4	5.252952159113421E-4	0.001031829888397279	0.0012982296050380313	1.1256326055243046E-4
150-154	0.006877615219753502	5.403036506516662E-4	5.290473245964232E-4	0.0010618467578779275	0.0013395028005739225	1.1256326055243046E-4
155-159	0.007035203784526903	5.478078680218282E-4	5.440557593367472E-4	0.0010881115186734945	0.0013807759961098137	1.1256326055243046E-4
160-164	0.007110245958228524	5.703205201323143E-4	5.440557593367472E-4	0.0011256326055243046	0.0013995365395352189	1.1256326055243046E-4
165-167	0.007179034617455009	5.815768461875574E-4	5.440557593367472E-4	0.001138139634474575	0.001407040756905381	1.3132380397783554E-4
>>END_MODULE
