##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479554
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6968951
Total Bases	1.1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.18708626305451	39.0	39.0	39.0	37.0	39.0
2	39.912723019576404	41.0	40.0	41.0	38.0	41.0
3	39.9663105681185	41.0	40.0	41.0	38.0	41.0
4	39.9930082734116	41.0	40.0	41.0	38.0	41.0
5	39.99679492652481	41.0	40.0	41.0	38.0	41.0
6	39.99524031665598	41.0	40.0	41.0	38.0	41.0
7	40.0229232491375	41.0	40.0	41.0	38.0	41.0
8	39.98872570635093	41.0	40.0	41.0	38.0	41.0
9	39.95562144144793	41.0	40.0	41.0	38.0	41.0
10-14	39.89728307746746	41.0	40.0	41.0	38.0	41.0
15-19	39.7563522831485	41.0	39.8	41.0	37.6	41.0
20-24	39.55630820190872	40.2	39.0	41.0	36.8	41.0
25-29	39.408084889677085	40.0	39.0	41.0	36.0	41.0
30-34	39.202925605302724	40.0	38.2	41.0	35.2	41.0
35-39	38.77727982303219	40.0	37.4	41.0	35.0	41.0
40-44	38.189657869599024	39.4	36.0	41.0	34.2	41.0
45-49	37.71481145440684	38.6	35.0	40.8	34.0	41.0
50-54	37.13440266691501	37.0	35.0	40.0	34.0	41.0
55-59	36.483311362068704	35.8	35.0	39.0	33.6	41.0
60-64	35.78730810418957	35.0	34.8	37.4	32.8	39.8
65-69	35.461612744873655	35.0	34.8	36.4	33.0	39.0
70-74	35.2602766183892	35.0	35.0	36.0	33.0	38.6
75-79	35.09128676611444	35.0	35.0	35.4	33.0	37.6
80-84	35.02263069434697	35.0	35.0	35.0	33.0	38.0
85-89	34.811304742995034	35.0	34.0	35.0	32.4	38.2
90-94	39.35450379485843	40.6	39.0	40.6	36.6	40.6
95-99	39.58565261035811	41.0	39.0	41.0	36.6	41.0
100-104	39.450087850442074	41.0	39.0	41.0	36.0	41.0
105-109	39.28222281364803	40.0	38.8	41.0	35.2	41.0
110-114	39.059769963231666	40.0	38.0	41.0	35.0	41.0
115-119	38.78622878202329	40.0	38.0	41.0	35.0	41.0
120-124	38.658212791686594	40.0	37.4	41.0	34.6	41.0
125-129	38.09440171637886	39.6	35.8	40.6	33.8	41.0
130-134	37.76744439536973	39.0	35.0	41.0	33.8	41.0
135-139	37.23464665784631	37.6	35.0	40.2	33.0	41.0
140-144	36.61497100979757	36.4	35.0	39.4	33.0	41.0
145-149	36.01995008216904	35.2	35.0	38.4	33.0	40.6
150-154	35.675949702266955	35.0	35.0	36.8	33.0	39.4
155-159	35.191949820485114	35.0	35.0	36.0	33.0	38.0
160-164	34.8264627722918	35.0	35.0	35.2	33.0	36.6
165-169	34.586008601183494	35.0	34.4	35.0	33.0	36.0
170-174	34.44816996588624	35.0	34.0	35.0	33.0	36.0
175-178	34.08276758729727	34.75	33.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	34.0
30	452.0
31	3339.0
32	18025.0
33	71667.0
34	218548.0
35	560267.0
36	1308695.0
37	2223465.0
38	2000049.0
39	554560.0
40	9849.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.37633755783331	27.140210915530904	27.827000075047163	22.656451451588623
2	23.20409484870822	27.174907672618158	27.719480306290002	21.90151717238362
3	21.56311617056857	27.0357762595834	28.65230362503625	22.74880394481178
4	22.038065700275407	26.340965806762025	28.855964118559598	22.765004374402977
5	22.672981916503645	26.908425672672976	27.57775165875036	22.840840752073017
6	22.427392587492722	27.162323282227124	27.733183946909655	22.677100183370495
7	22.500717826829316	27.094092066366947	27.490034009422654	22.915156097381086
8	22.62234301833949	27.308557629405055	27.30585994936684	22.763239402888612
9	22.593931281766796	27.42481615956261	27.431560359658146	22.549692199012448
10-14	22.64842441853874	27.16024549462322	27.30609240902971	22.885237677808323
15-19	22.739301797358024	27.074961497074668	27.259083899427615	22.926652806139693
20-24	22.770930660869908	27.031655122844167	27.192772628190383	23.004641588095538
25-29	22.86484723454075	26.96051959613434	27.057707824319614	23.116925345005296
30-34	22.859371518037648	27.022675292163772	27.04233391797417	23.07561927182441
35-39	22.836065284430898	27.04095063948649	27.039426737252136	23.08355733883048
40-44	22.913041001436227	26.989548355268965	26.9084830701206	23.18892757317421
45-49	23.115028359361403	26.9053577790976	26.86813553431499	23.111478327226006
50-54	23.10173511049224	26.943142518866896	26.858065152129782	23.09705721851108
55-59	23.066137213477322	26.893307184969444	26.852443072135245	23.188112529417985
60-64	23.017174320783717	26.93094843111969	26.918811740820104	23.13306550727649
65-69	22.99748986612189	26.945863157884165	26.91529040740852	23.14135656858543
70-74	22.957168159167715	26.90846585088631	26.900424468474526	23.23394152147145
75-79	22.935044312981965	26.91254680941221	26.9511666820444	23.201242195561427
80-84	22.916671389998296	26.95127573719488	26.92385410659366	23.208198766213165
85-89	22.90307393465674	26.88069983559936	26.885171096769085	23.33105513297482
90-94	22.324599030493676	27.024708158444653	27.95290001465469	22.697792796406972
95-99	22.48779874051812	27.282348001220015	27.384998902818214	22.844854355443655
100-104	22.632697950766058	27.18683793487938	27.266553949533147	22.91391016482141
105-109	22.653337055685267	27.092528440371222	27.216260924514064	23.037873579429448
110-114	22.713356121084	27.02769141694547	27.16227176210732	23.09668069986321
115-119	22.780297067087226	27.09640042188789	27.000201032882583	23.123101478142303
120-124	22.77617799611295	27.068423722733936	26.997273968897694	23.158124312255417
125-129	22.778975467063407	27.08217284328934	27.008556233351904	23.13029545629535
130-134	22.891844095023515	27.10950760877364	26.87356786083553	23.12508043536731
135-139	22.99915365259517	27.047916610615978	26.82643610785102	23.126493628937826
140-144	22.939517474935624	27.158053367412215	26.86856791026031	23.03386124739185
145-149	22.85394401994864	27.236852507627724	26.92207230130077	22.98713117112286
150-154	22.78910445016901	27.28781576386942	26.98985724261749	22.93322254334408
155-159	22.723899255109306	27.322707653923885	27.109979772792897	22.843413318173912
160-164	22.67417844185136	27.447927349608598	27.10416859194411	22.77372561659593
165-169	22.568574188365584	27.4481394275845	27.181872885196963	22.801413498852956
170-174	22.513558379237907	27.58562741077144	27.232325833996164	22.66848837599449
175-178	22.478204895111187	27.664789746712575	27.17868592342744	22.678319434748797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	1.5
9	1.5
10	4.5
11	11.0
12	41.5
13	84.5
14	129.0
15	284.0
16	535.5
17	978.5
18	1742.0
19	2955.0
20	4874.0
21	7509.0
22	11184.5
23	15973.5
24	21936.5
25	29210.0
26	37769.5
27	47546.0
28	58393.5
29	70085.0
30	82719.0
31	96422.0
32	111175.0
33	126372.0
34	141643.0
35	157984.0
36	174665.0
37	191225.5
38	207722.5
39	223001.0
40	236722.5
41	249254.0
42	259597.5
43	267220.0
44	272193.5
45	273070.5
46	270167.0
47	264105.5
48	255587.0
49	245117.0
50	233182.5
51	220020.0
52	206554.0
53	193021.5
54	179534.0
55	167110.0
56	154890.5
57	143275.5
58	132882.0
59	123267.5
60	113381.0
61	104024.5
62	95028.0
63	85547.0
64	75928.0
65	66384.5
66	56786.5
67	47382.0
68	38820.0
69	31137.0
70	24299.0
71	18265.5
72	13499.0
73	9775.0
74	6737.0
75	4526.5
76	2961.0
77	1855.5
78	1150.5
79	686.5
80	402.0
81	218.0
82	102.5
83	58.5
84	32.0
85	12.5
86	6.5
87	4.0
88	2.5
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1448.0
92-93	3224.0
94-95	4888.0
96-97	6461.0
98-99	8031.0
100-101	9420.0
102-103	10828.0
104-105	12040.0
106-107	12941.0
108-109	14233.0
110-111	15266.0
112-113	16152.0
114-115	16512.0
116-117	17269.0
118-119	18453.0
120-121	19215.0
122-123	20208.0
124-125	21353.0
126-127	21482.0
128-129	22245.0
130-131	23870.0
132-133	25851.0
134-135	100961.0
136-137	99271.0
138-139	99055.0
140-141	101811.0
142-143	99253.0
144-145	103506.0
146-147	106494.0
148-149	84538.0
150-151	80728.0
152-153	108254.0
154-155	80970.0
156-157	90219.0
158-159	84122.0
160-161	84583.0
162-163	84213.0
164-165	84712.0
166-167	94508.0
168-169	97142.0
170-171	98263.0
172-173	101445.0
174-175	102574.0
176-177	294296.0
178-179	4366643.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.29545673435156
#Duplication Level	Percentage of total
1	94.767617082946
2	4.7982745651821475
3	0.31029496986710875
4	0.06638674033965387
5	0.02880269510785861
6	0.009324319407392727
7	0.0031303136420119757
8	0.003152624537723956
9	0.0
>10	0.0066732571111265465
>50	0.0016164825026920198
>100	0.0047269493562662225
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.4349361905400109E-5	1.4349361905400109E-5	0.0	0.0	0.0
2	5.7397447621600436E-5	1.4349361905400109E-5	1.4349361905400109E-5	1.4349361905400109E-5	0.0	0.0
3	5.7397447621600436E-5	2.8698723810800218E-5	1.4349361905400109E-5	1.4349361905400109E-5	1.4349361905400109E-5	0.0
4	7.174680952700054E-5	2.8698723810800218E-5	1.4349361905400109E-5	1.4349361905400109E-5	1.4349361905400109E-5	0.0
5	7.174680952700054E-5	5.7397447621600436E-5	4.304808571620033E-5	2.8698723810800218E-5	1.4349361905400109E-5	0.0
6	8.609617143240066E-5	5.7397447621600436E-5	4.304808571620033E-5	2.8698723810800218E-5	2.8698723810800218E-5	0.0
7	8.609617143240066E-5	7.174680952700054E-5	4.304808571620033E-5	2.8698723810800218E-5	2.8698723810800218E-5	0.0
8	8.609617143240066E-5	7.174680952700054E-5	4.304808571620033E-5	2.8698723810800218E-5	2.8698723810800218E-5	0.0
9	8.609617143240066E-5	8.609617143240066E-5	4.304808571620033E-5	2.8698723810800218E-5	5.7397447621600436E-5	0.0
10-14	9.183591619456072E-5	1.5210323619724119E-4	6.026732000268045E-5	4.878783047836037E-5	6.60070647648405E-5	0.0
15-19	1.0044553333780077E-4	1.9802119429452155E-4	7.461668190808057E-5	8.609617143240066E-5	7.174680952700054E-5	0.0
20-24	1.0044553333780077E-4	2.0089106667560153E-4	8.609617143240066E-5	1.7219234286480132E-4	7.461668190808057E-5	0.0
25-29	1.0331540571888078E-4	2.2385004572424166E-4	9.757566095672077E-5	2.0089106667560153E-4	9.183591619456069E-5	0.0
30-34	1.176647676242809E-4	2.468090247728819E-4	1.0905515048104083E-4	2.3819940762964182E-4	1.2053464000536093E-4	0.0
35-39	1.34884001910761E-4	2.7263787620260206E-4	1.5210323619724116E-4	2.61158386678282E-4	1.7219234286480132E-4	2.8698723810800217E-6
40-44	1.664525981026413E-4	2.8698723810800217E-4	1.808019600080414E-4	2.783776209647621E-4	2.1237055619992162E-4	2.8698723810800218E-5
45-49	2.525487695350419E-4	2.955968552512423E-4	2.1237055619992162E-4	3.156859619188024E-4	2.5828851429720196E-4	3.156859619188024E-5
50-54	2.8698723810800217E-4	3.0420647239448237E-4	2.410692800107218E-4	3.8169302668364293E-4	3.185558342998824E-4	6.313719238376048E-5
55-59	3.0133660001340233E-4	3.415148133485226E-4	2.525487695350419E-4	4.304808571620033E-4	3.4438468572960265E-4	8.035642667024062E-5
60-64	3.329051962052826E-4	4.046520057322831E-4	2.7263787620260206E-4	4.4770009144848343E-4	4.0178213335120307E-4	8.609617143240066E-5
65-69	3.4438468572960265E-4	4.190013676376832E-4	2.8698723810800217E-4	4.706590704971236E-4	4.964879219268438E-4	8.609617143240066E-5
70-74	5.739744762160044E-4	4.304808571620033E-4	3.1281608953772245E-4	5.07967411451164E-4	5.538853695484442E-4	8.609617143240066E-5
75-79	0.0038714578420769494	4.304808571620033E-4	3.185558342998824E-4	5.481456247862841E-4	5.797142209781645E-4	1.0331540571888078E-4
80-84	0.005226037605946719	4.5056996382956336E-4	3.3003532382420254E-4	5.911937105024846E-4	7.088584781267654E-4	1.3775387429184103E-4
85-89	0.00526047607451968	5.022276666890039E-4	3.386449409674426E-4	6.170225619322047E-4	9.097495448023669E-4	1.4349361905400108E-4
90-94	0.005315003649760201	5.22316773356564E-4	3.644737923971628E-4	6.371116685997648E-4	9.327085238510071E-4	1.4349361905400108E-4
95-99	0.005352311990714241	5.452757524052041E-4	3.845628990647229E-4	6.65810392410565E-4	9.556675028996473E-4	1.4349361905400108E-4
100-104	0.005366661352619642	5.653648590727643E-4	3.9030264382688295E-4	6.744200095538051E-4	9.671469924239673E-4	1.4349361905400108E-4
105-109	0.005398229948811522	5.998033276457247E-4	4.2187124001876326E-4	6.858994990781252E-4	9.814963543293676E-4	1.4349361905400108E-4
110-114	0.005424058800241241	6.026732000268047E-4	4.304808571620033E-4	6.973789886024453E-4	9.929758438536878E-4	1.4349361905400108E-4
115-119	0.005432668417384481	6.198924343132847E-4	4.4770009144848343E-4	7.232078400321656E-4	0.001015934822902328	1.4349361905400108E-4
120-124	0.005510154971673642	6.313719238376049E-4	4.907481771646838E-4	7.834751600348459E-4	0.0010503732914752882	1.4349361905400108E-4
125-129	0.005596251143106043	6.313719238376049E-4	5.280565181187241E-4	8.179136286078062E-4	0.0010819418876671682	1.4349361905400108E-4
130-134	0.005659388335489803	6.399815409808449E-4	5.682347314538443E-4	8.293931181321265E-4	0.0011106406114779685	1.4349361905400108E-4
135-139	0.005711046038349244	6.54330902886245E-4	6.170225619322047E-4	8.638315867050865E-4	0.001153688697194169	1.4349361905400108E-4
140-144	0.005857409529784324	6.629405200294851E-4	6.60070647648405E-4	8.839206933726468E-4	0.0011823874210049692	1.5210323619724116E-4
145-149	0.006075519830746406	6.744200095538051E-4	6.858994990781252E-4	9.327085238510071E-4	0.0012225656343400895	1.5784298095940122E-4
150-154	0.006221883322181487	6.887693714592053E-4	7.117283505078455E-4	9.843662267104477E-4	0.0012570041029130498	1.5784298095940122E-4
155-159	0.0062936301317084884	6.887693714592053E-4	7.318174571754056E-4	0.0010101950781401677	0.0013115316781535699	1.5784298095940122E-4
160-164	0.006371116685997649	6.887693714592053E-4	7.461668190808057E-4	0.0010245444400455678	0.0013660592533940904	1.5784298095940122E-4
165-167	0.006418947892348983	6.935524920943388E-4	7.461668190808057E-4	0.0010379371778239412	0.0014301530699048777	1.5784298095940122E-4
>>END_MODULE
