##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479562
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14024500
Total Bases	2.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.22700795037256	39.0	39.0	39.0	37.0	39.0
2	39.955657100074866	41.0	40.0	41.0	38.0	41.0
3	40.00568883026133	41.0	40.0	41.0	38.0	41.0
4	40.03114642233235	41.0	40.0	41.0	38.0	41.0
5	40.03589553994795	41.0	40.0	41.0	38.0	41.0
6	40.037557346072944	41.0	40.0	41.0	38.0	41.0
7	40.06650518735071	41.0	40.0	41.0	38.0	41.0
8	40.03612898855574	41.0	40.0	41.0	38.0	41.0
9	40.00590816071874	41.0	40.0	41.0	38.0	41.0
10-14	39.95357634140255	41.0	40.0	41.0	38.0	41.0
15-19	39.82820601090948	41.0	40.0	41.0	38.0	41.0
20-24	39.65334148097971	40.8	39.4	41.0	37.0	41.0
25-29	39.5368694356305	40.4	39.0	41.0	36.6	41.0
30-34	39.38672836821277	40.0	38.8	41.0	35.6	41.0
35-39	39.043146693286744	40.0	38.0	41.0	35.0	41.0
40-44	38.546916367784945	40.0	36.8	41.0	34.8	41.0
45-49	38.12776729295162	39.0	35.8	41.0	34.6	41.0
50-54	37.55060977574958	37.8	35.0	40.4	34.0	41.0
55-59	36.841733224000855	36.6	35.0	39.4	34.0	41.0
60-64	36.03970477378873	35.4	34.8	38.0	33.4	40.2
65-69	35.59654302114158	35.0	34.8	36.8	33.0	39.0
70-74	35.307542985489675	35.0	35.0	36.0	33.0	38.2
75-79	35.09351229633855	35.0	35.0	35.6	33.0	37.0
80-84	35.00739404613355	35.0	35.0	35.0	33.0	37.0
85-89	34.79024434382687	35.0	34.6	35.0	32.4	37.6
90-94	39.43722160517562	40.6	39.8	40.6	36.8	40.6
95-99	39.69649596709702	41.0	39.8	41.0	37.0	41.0
100-104	39.5816279656046	41.0	39.0	41.0	36.4	41.0
105-109	39.43292355714757	40.6	39.0	41.0	36.0	41.0
110-114	39.236422115688406	40.0	38.4	41.0	35.2	41.0
115-119	38.998151337586606	40.0	38.0	41.0	35.0	41.0
120-124	38.92529305439102	40.0	38.0	41.0	35.0	41.0
125-129	38.4263848592429	39.6	36.8	40.6	34.4	41.0
130-134	38.172132327513275	39.4	35.8	41.0	34.0	41.0
135-139	37.68455989416729	38.6	35.0	40.8	34.0	41.0
140-144	37.05829576914627	37.2	35.0	40.0	33.2	41.0
145-149	36.39712529980007	36.0	35.0	39.0	33.0	41.0
150-154	35.9350778712045	35.0	35.0	37.4	33.6	39.6
155-159	35.354412914623694	35.0	35.0	36.2	33.0	38.2
160-164	34.925534161766436	35.0	35.0	35.6	33.0	36.8
165-169	34.65799425046917	35.0	35.0	35.0	33.0	36.0
170-174	34.51504231243135	35.0	34.6	35.0	33.0	36.0
175-178	34.159988501941285	34.75	33.75	35.0	32.0	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	4.0
29	119.0
30	974.0
31	7021.0
32	35871.0
33	135510.0
34	389174.0
35	940621.0
36	2150246.0
37	4056183.0
38	4897665.0
39	1399015.0
40	12097.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.81438197440194	27.826988484437948	28.43567328603515	21.92295625512496
2	22.429020642447146	27.967827730043854	28.363720631751576	21.239430995757424
3	20.916125352062462	27.68604228314735	29.3297372455346	22.068095119255588
4	21.372868907982458	27.01592926664052	29.509879140076293	22.101322685300723
5	21.93835074334201	27.60211772255695	28.347991015722485	22.111540518378554
6	21.803016150308387	27.77958572498128	28.429013512068167	21.98838461264216
7	21.808620628186386	27.771656743555923	28.16004848657706	22.25967414168063
8	21.906470818924024	28.032108096545333	28.01378302256765	22.047638061962992
9	21.943377660522657	28.092716317872295	28.092702057114337	21.87120396449071
10-14	22.003827587436273	27.880390744768086	27.951111269563977	22.164670398231667
15-19	22.087606688295484	27.788025241541586	27.91189703732753	22.212471032835396
20-24	22.1108588541481	27.720761524475023	27.886184890726938	22.282194730649934
25-29	22.190612143035402	27.684740275945668	27.722076366358873	22.402571214660057
30-34	22.19794359870227	27.720337979963634	27.738443438268746	22.34327498306535
35-39	22.167438411351565	27.74758601019644	27.74054975221933	22.344425826232666
40-44	22.231211094869693	27.689372170130845	27.63745873293166	22.44195800206781
45-49	22.393487111839995	27.63349852044636	27.580501265642273	22.392513102071376
50-54	22.373666084352383	27.681557274769155	27.592196513244676	22.352580127633782
55-59	22.334236514670756	27.609336518235946	27.595413740240293	22.46101322685301
60-64	22.2993689614603	27.657938607436982	27.62901351206817	22.413678919034545
65-69	22.280032799743307	27.642064957752506	27.64923669293023	22.42866554957396
70-74	22.239014581625014	27.623339156476167	27.653392277799565	22.484253984099254
75-79	22.224063602980497	27.610093764483583	27.679810331919143	22.486032300616777
80-84	22.19657884416557	27.651290242076364	27.66650789689472	22.485623016863347
85-89	22.18595885771329	27.597053727405612	27.605078255909298	22.611909158971798
90-94	21.685634090088946	27.70222469635284	28.618678127583692	21.993463085974522
95-99	21.815895154405286	27.95846010389359	28.079559124098267	22.14608561760286
100-104	21.99699570287009	27.908170789080415	27.883952007985496	22.210881500063998
105-109	22.009176474191538	27.806895532042798	27.868522349583547	22.315405644182114
110-114	22.0633474743869	27.720134880728924	27.829759630174923	22.386758014709248
115-119	22.13843823114534	27.786546628523322	27.68530467914169	22.389710461189644
120-124	22.137449033671725	27.76183176340598	27.66285273099531	22.43786647192698
125-129	22.131366761745905	27.769615945432648	27.698648771209943	22.400368521611504
130-134	22.224398749198855	27.796464436576144	27.590817361238095	22.388319452986906
135-139	22.29834094936199	27.75286403738184	27.539513074390324	22.409281938865846
140-144	22.268753113534252	27.81953545768225	27.57625035325193	22.33546107553157
145-149	22.196509133395388	27.88979468454936	27.60820899415967	22.305487187895576
150-154	22.1509500128009	27.916974703319813	27.666683145833197	22.26539213804609
155-159	22.096380438422898	27.945206936939528	27.750638080595895	22.20777454404168
160-164	22.06645609813929	28.050947587044085	27.749591511583464	22.13300480323316
165-169	21.970808049860675	28.051541082081886	27.805468581416186	22.172182286641252
170-174	21.91385606983271	28.146463015282215	27.866799866877212	22.072881048007858
175-178	21.908594569853086	28.210110740041074	27.789205737274	22.092088952831844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	1.0
9	1.0
10	3.0
11	9.5
12	14.0
13	31.5
14	71.0
15	159.5
16	340.0
17	660.0
18	1284.0
19	2388.0
20	4182.0
21	7048.5
22	11521.5
23	18057.0
24	27369.5
25	40416.5
26	57097.0
27	78311.5
28	105022.0
29	135843.5
30	170195.5
31	208406.5
32	249581.0
33	294161.0
34	341511.5
35	389966.0
36	437835.5
37	483446.0
38	527340.5
39	567668.0
40	600768.0
41	624172.0
42	638006.0
43	641200.5
44	633812.0
45	617153.5
46	589412.0
47	553389.0
48	512748.5
49	469119.5
50	425931.0
51	383180.5
52	342999.5
53	306771.5
54	275062.0
55	249581.5
56	227984.0
57	209983.5
58	195178.0
59	181523.0
60	168952.0
61	156752.5
62	143964.0
63	130398.5
64	115424.5
65	100388.5
66	85623.0
67	71030.0
68	57571.5
69	45211.0
70	34315.5
71	25321.0
72	18190.0
73	12623.0
74	8485.0
75	5550.5
76	3498.0
77	2185.0
78	1288.5
79	736.0
80	385.0
81	205.0
82	113.5
83	47.5
84	21.5
85	6.0
86	3.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2461.0
92-93	5225.0
94-95	8006.0
96-97	10485.0
98-99	13095.0
100-101	15346.0
102-103	17505.0
104-105	19684.0
106-107	21276.0
108-109	23315.0
110-111	25374.0
112-113	26618.0
114-115	27380.0
116-117	28552.0
118-119	30596.0
120-121	32205.0
122-123	33390.0
124-125	36073.0
126-127	35764.0
128-129	36809.0
130-131	39287.0
132-133	42630.0
134-135	173218.0
136-137	172567.0
138-139	171629.0
140-141	176124.0
142-143	169377.0
144-145	179345.0
146-147	186036.0
148-149	150646.0
150-151	143084.0
152-153	203649.0
154-155	145094.0
156-157	162349.0
158-159	149284.0
160-161	149370.0
162-163	150847.0
164-165	151028.0
166-167	170704.0
168-169	176729.0
170-171	177902.0
172-173	183574.0
174-175	185586.0
176-177	557769.0
178-179	9407513.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.49322897952102
#Duplication Level	Percentage of total
1	89.51771810095649
2	9.093380901148219
3	1.0882642660180806
4	0.18892939091097735
5	0.04866834080825534
6	0.03663138437364895
7	0.009190454957355963
8	0.006148760325513514
9	0.003085300893422322
>10	0.006225588629821018
>50	0.0
>100	0.0017575109781938546
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.130378979642768E-6	0.0	1.4260757959285536E-5	1.4260757959285536E-5	0.0	0.0
2	4.991265285749938E-5	0.0	2.1391136938928305E-5	2.1391136938928305E-5	0.0	0.0
3	5.7043031837142144E-5	1.4260757959285536E-5	2.1391136938928305E-5	2.8521515918571072E-5	0.0	0.0
4	5.7043031837142144E-5	1.4260757959285536E-5	2.8521515918571072E-5	2.8521515918571072E-5	0.0	0.0
5	5.7043031837142144E-5	2.1391136938928305E-5	2.8521515918571072E-5	2.8521515918571072E-5	0.0	0.0
6	5.7043031837142144E-5	2.1391136938928305E-5	3.565189489821384E-5	2.8521515918571072E-5	0.0	0.0
7	6.417341081678491E-5	2.1391136938928305E-5	3.565189489821384E-5	4.278227387785661E-5	0.0	0.0
8	6.417341081678491E-5	2.1391136938928305E-5	5.7043031837142144E-5	4.278227387785661E-5	0.0	0.0
9	6.417341081678491E-5	2.1391136938928305E-5	7.130378979642769E-5	5.7043031837142144E-5	7.130378979642768E-6	0.0
10-14	6.702556240864201E-5	4.278227387785661E-5	9.126885093942744E-5	8.27123961638561E-5	8.556454775571321E-6	0.0
15-19	7.9860244571999E-5	7.843416877607046E-5	1.3690327640914114E-4	1.3405112481728403E-4	1.4260757959285538E-5	0.0
20-24	8.556454775571322E-5	9.2694926735356E-5	1.85389853470712E-4	2.0392883881778317E-4	1.85389853470712E-5	0.0
25-29	9.269492673535598E-5	1.0838176049057007E-4	2.210417483689258E-4	2.310242789404257E-4	3.1373667510428184E-5	0.0
30-34	1.0267745730685586E-4	1.2549467004171273E-4	2.623979464508539E-4	2.595457948589968E-4	5.133872865342793E-5	1.4260757959285537E-6
35-39	1.2834682163356982E-4	1.4545973118471246E-4	3.023280687368533E-4	2.6667617383863953E-4	9.412100253128454E-5	1.996506114299975E-5
40-44	1.354772006132126E-4	1.525901101643552E-4	3.265713572676388E-4	2.909194623694249E-4	1.3690327640914114E-4	6.274733502085635E-5
45-49	1.4973795857249812E-4	1.6257264073585512E-4	3.5509287318620986E-4	3.222931298798531E-4	1.7825947449106919E-4	1.2834682163356982E-4
50-54	1.7255517130735498E-4	1.9252023245035473E-4	3.9359691967628084E-4	3.636493279617812E-4	2.067809904096403E-4	1.8966808085849762E-4
55-59	1.8538985347071196E-4	2.4243288530785412E-4	4.321009661663517E-4	4.035794502477806E-4	2.310242789404257E-4	2.324503547363542E-4
60-64	1.9109415665442616E-4	2.5384149167528256E-4	4.706050126564227E-4	4.492138757174944E-4	2.7238047702235376E-4	2.980498413490677E-4
65-69	1.9822453563406896E-4	2.7523262861421085E-4	4.962743769831368E-4	4.8201361902385116E-4	3.1801490249206744E-4	3.4511034261470997E-4
70-74	2.8378908338978217E-4	2.866412349816393E-4	5.547434846162073E-4	5.091090591464937E-4	3.5937110057399546E-4	3.8504046490070947E-4
75-79	0.0018096901850333344	3.037541445327819E-4	6.26047274412635E-4	5.219437413098505E-4	4.235445113907804E-4	4.0072729865592353E-4
80-84	0.0025384149167528253	3.0803237192056755E-4	6.54568790331206E-4	5.461870298406361E-4	5.419088024528503E-4	4.135619808192805E-4
85-89	0.0025512495989161825	3.265713572676388E-4	6.716816998823487E-4	5.747085457592071E-4	8.27123961638561E-4	4.135619808192805E-4
90-94	0.0025697885842632536	3.6650147955363826E-4	6.987771400049911E-4	6.117865164533495E-4	8.513672501693464E-4	4.135619808192805E-4
95-99	0.002584049342222539	4.0215337445185205E-4	7.372811864950621E-4	6.374558807800636E-4	8.599237049449176E-4	4.135619808192805E-4
100-104	0.0025954579485899677	4.321009661663517E-4	7.700809298014189E-4	6.830903062497772E-4	8.699062355164176E-4	4.135619808192805E-4
105-109	0.0026097187065492527	4.563442546971372E-4	7.857677635566331E-4	7.31576883311348E-4	8.756105387001318E-4	4.135619808192805E-4
110-114	0.002619701237120753	4.606224820849228E-4	8.100110520874185E-4	7.914720667403473E-4	8.841669934757032E-4	4.1926628400299474E-4
115-119	0.002636814146671896	4.8771792220756534E-4	8.527933259652752E-4	8.27123961638561E-4	8.841669934757032E-4	4.206923597989233E-4
120-124	0.002666761738386395	4.977004527790651E-4	9.112624335983457E-4	8.55645477557132E-4	8.94149524047203E-4	4.235445113907804E-4
125-129	0.002699561481692752	5.347784234732076E-4	9.483404042924881E-4	8.88445220863489E-4	9.383578737209884E-4	4.392313451459945E-4
130-134	0.0027352133765909657	5.533174088202788E-4	9.626011622517738E-4	9.155406609861313E-4	9.825662233947734E-4	4.506399515134229E-4
135-139	0.002769439195693251	5.561695604121359E-4	9.911226781703448E-4	9.497664800884167E-4	0.001016792042497059	4.763093158401369E-4
140-144	0.002807943242183322	5.632999393917787E-4	0.0010253484972726301	9.754358444151307E-4	0.0010267745730685586	4.991265285749938E-4
145-149	0.0028350386823059646	5.775606973510642E-4	0.0010552960889871296	9.982530571499876E-4	0.001063852543762701	5.190915897179936E-4
150-154	0.0028778209561838215	5.832650005347784E-4	0.001086669756497558	0.0010267745730685586	0.0011066348176405575	5.276480444935648E-4
155-159	0.0029248814574494636	6.117865164533495E-4	0.0011251738029876286	0.001041035331027844	0.0011536953189062	5.276480444935648E-4
160-164	0.002964811579735463	6.174908196370638E-4	0.0011451388641306286	0.00104531355841563	0.001223573032906699	5.333523476772791E-4
165-167	0.0030113967224024625	6.393573151746349E-4	0.0011622517736817712	0.0010600496749735581	0.0012501931144306986	5.442855954460647E-4
>>END_MODULE
