##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479595
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11861422
Total Bases	1.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.90888402756432	41.0	40.0	41.0	38.0	41.0
2	40.016608716897515	41.0	40.0	41.0	38.0	41.0
3	40.064855040146114	41.0	40.0	41.0	38.0	41.0
4	40.06968886192566	41.0	40.0	41.0	38.0	41.0
5	40.05048947756854	41.0	40.0	41.0	38.0	41.0
6	40.03192365974333	41.0	40.0	41.0	38.0	41.0
7	39.9986537027348	41.0	40.0	41.0	38.0	41.0
8	39.979627822026735	41.0	40.0	41.0	38.0	41.0
9	39.94287396570158	41.0	40.0	41.0	38.0	41.0
10-14	39.84163494056615	40.8	40.0	41.0	38.0	41.0
15-19	39.745871666989	40.8	40.0	41.0	37.0	41.0
20-24	39.51623903103692	40.4	39.0	41.0	36.2	41.0
25-29	39.05324417257897	40.0	38.0	41.0	35.0	41.0
30-34	38.43688097430477	39.6	36.4	41.0	35.0	41.0
35-39	37.78638087406384	38.8	35.0	40.8	34.0	41.0
40-44	37.122527265280674	36.8	35.0	39.8	34.0	41.0
45-49	36.36501483548936	35.4	35.0	38.8	33.6	40.8
50-54	35.75407034670885	35.0	35.0	37.0	33.0	39.4
55-59	35.299018229011665	35.0	35.0	36.0	33.0	38.8
60-64	34.60602106560242	34.8	33.8	35.0	32.2	37.0
65-69	35.003736533444304	35.0	35.0	35.0	33.0	36.8
70-74	34.95356411735457	35.0	35.0	35.0	33.0	36.0
75-79	34.94126454652739	35.0	34.8	35.0	33.0	36.6
80-84	34.93310950407126	35.0	34.0	35.0	33.0	37.6
85-89	35.728642282518905	36.0	35.0	36.2	33.4	38.8
90-94	39.88002666172642	41.0	40.0	41.0	37.4	41.0
95-99	39.72660628587495	41.0	40.0	41.0	36.6	41.0
100-104	39.51741701262587	41.0	39.2	41.0	35.4	41.0
105-109	39.361903426457275	40.8	39.0	41.0	35.0	41.0
110-114	39.078388251081435	40.2	37.8	41.0	35.0	41.0
115-119	38.5929936853146	40.0	36.4	41.0	35.0	41.0
120-124	37.48749088715632	38.4	34.8	40.2	33.8	40.6
125-129	37.26144901943984	37.4	35.0	40.0	34.0	41.0
130-134	36.6329733843854	35.8	35.0	39.2	33.8	41.0
135-139	35.93822013521394	35.0	35.0	37.6	33.0	40.0
140-144	35.48915665600513	35.0	35.0	36.4	33.6	38.8
145-149	35.12667773098609	35.0	35.0	35.4	34.0	37.0
150-154	34.83876566965246	35.0	35.0	35.0	33.2	36.0
155-159	34.66770389408366	35.0	35.0	35.0	33.0	36.0
160-164	34.56912993953589	35.0	35.0	35.0	33.0	35.0
165-169	34.497623735202446	35.0	35.0	35.0	33.0	35.0
170-174	34.4418500331032	35.0	34.6	35.0	33.0	35.0
175-176	33.78396903873838	34.5	33.5	35.0	31.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	13.0
30	375.0
31	4441.0
32	26958.0
33	113085.0
34	388268.0
35	1310358.0
36	3422518.0
37	4374498.0
38	2137474.0
39	82972.0
40	462.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.051591621982595	24.683912266168424	25.251845857941824	25.012650253907164
2	24.677504939964194	24.732633237397675	25.550815070908023	25.039046751730105
3	24.69703042350234	24.89381121420349	25.37243005096691	25.03672831132726
4	24.727895188283497	25.015449243775322	25.289320285544175	24.967335282397002
5	24.826922100908305	25.00706070486321	25.2594672038479	24.90654999038058
6	24.66409170839719	24.93222144865936	25.334997776826423	25.068689066117027
7	24.70079894299351	24.910925519722678	25.353292379277963	25.034983158005847
8	24.869539250858793	24.810979661629105	25.307319813762636	25.012161273749474
9	24.718882778135708	24.669504212901288	25.236830794823756	25.374782214139245
10-14	24.888531914638904	24.72896420007652	25.173425243617505	25.209078641667077
15-19	24.926810630293737	24.68542473238032	25.105720039300515	25.282044598025433
20-24	24.96647029335943	24.650759411476972	25.036399514324675	25.346370780838924
25-29	24.946621071234123	24.754983002881104	25.01933747909821	25.279058446786568
30-34	24.890644646147823	24.66724478734506	25.05041301118871	25.39169755531841
35-39	25.006377818780916	24.708497851269435	24.959811732522457	25.325312597427192
40-44	25.056022793894357	24.62466136016407	24.90570354886623	25.413612297075343
45-49	25.21119643159142	24.613829606601975	24.79161098896911	25.383362972837492
50-54	25.203721779732653	24.56467192550775	24.815189949400672	25.41641634535893
55-59	25.1989550662644	24.66165861057806	24.764169085291798	25.375217237865748
60-64	25.108675840046836	24.636452526518323	24.842603188723917	25.41226844471093
65-69	25.131796170813246	24.67182771171956	24.788361800128182	25.408014317339017
70-74	25.097469763743334	24.7297280208056	24.73596841930082	25.436833796150243
75-79	25.07845012174763	24.748631319246545	24.698691269900017	25.474227289105812
80-84	25.091392920680168	24.82149273501946	24.64048745588851	25.44662688841186
85-89	25.125051616914057	24.822168876547853	24.606056508233163	25.446722998304928
90-94	24.877218413533683	25.197487114972244	24.972732208047177	24.952562263446897
95-99	24.931120267160704	25.073801402150664	24.973861968230093	25.02121636245854
100-104	24.96396202370919	25.059090910321512	24.911197810417978	25.065749255551317
105-109	25.074265028268066	24.914979229893635	24.8242945951014	25.1864611467369
110-114	25.15460840666499	24.969371185858442	24.66810812752116	25.207912279955412
115-119	25.070533463243088	24.989043868770338	24.729327011405225	25.211095656581346
120-124	25.05211318765311	24.989975224249445	24.72893731943841	25.228974268659037
125-129	25.06250161674906	25.04711396977706	24.677665194404824	25.212719219069047
130-134	25.180425940245517	24.914702431310435	24.617149248017192	25.287722380426857
135-139	25.243605185626556	25.028286326483563	24.563551419031533	25.16455706885835
140-144	25.162844710006908	25.0330219131829	24.655219562724305	25.148913814085883
145-149	25.147741524661406	25.156460365154427	24.669607255051893	25.02619085513228
150-154	25.05245772578833	25.159296896132876	24.80992753756602	24.97831784051278
155-159	25.020933483150387	25.353856437108398	24.75264842260149	24.872561657139727
160-164	24.911533023720487	25.40586621830223	24.80933148427155	24.873269273705738
165-169	24.918556123356	25.56162571415624	24.801190928516405	24.718627233971354
170-174	24.80226028706779	25.726960850466046	24.868790749196528	24.601988113269638
175-176	24.784832268987877	25.77826543000434	24.789093016136636	24.64780928487115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.5
15	0.5
16	0.5
17	1.0
18	4.0
19	8.0
20	10.5
21	34.0
22	92.0
23	220.0
24	489.0
25	1077.5
26	2173.0
27	4239.0
28	7744.0
29	13490.0
30	22279.0
31	34652.0
32	51291.5
33	73575.0
34	102009.5
35	135770.5
36	174873.0
37	218542.5
38	264218.5
39	309732.0
40	353612.0
41	393662.0
42	428184.5
43	453805.0
44	470847.0
45	479401.5
46	477609.5
47	467798.0
48	452375.5
49	432437.0
50	410337.0
51	387942.5
52	365838.5
53	347292.5
54	333417.0
55	323302.5
56	316795.5
57	313559.5
58	313383.0
59	312837.5
60	309207.0
61	303801.0
62	294121.0
63	278207.0
64	257543.5
65	232659.0
66	204005.5
67	174023.5
68	144142.5
69	115424.0
70	89565.5
71	67062.5
72	48467.0
73	33797.0
74	22772.0
75	14814.0
76	9294.5
77	5623.5
78	3256.5
79	1830.5
80	1006.5
81	528.0
82	272.5
83	130.0
84	60.5
85	27.0
86	10.5
87	4.0
88	1.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1627.0
92-93	3853.0
94-95	6002.0
96-97	8189.0
98-99	10065.0
100-101	11605.0
102-103	12982.0
104-105	15325.0
106-107	16379.0
108-109	17624.0
110-111	18556.0
112-113	19020.0
114-115	20062.0
116-117	21307.0
118-119	22588.0
120-121	23672.0
122-123	25202.0
124-125	26564.0
126-127	28287.0
128-129	29702.0
130-131	31570.0
132-133	89002.0
134-135	144590.0
136-137	144465.0
138-139	151605.0
140-141	135020.0
142-143	138245.0
144-145	130439.0
146-147	147408.0
148-149	127069.0
150-151	115433.0
152-153	116420.0
154-155	122742.0
156-157	123613.0
158-159	127937.0
160-161	132650.0
162-163	140283.0
164-165	148208.0
166-167	151868.0
168-169	157316.0
170-171	161018.0
172-173	168540.0
174-175	496431.0
176-177	8120939.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.43281903491874
#Duplication Level	Percentage of total
1	82.36305375051926
2	14.022647584346362
3	2.4863683604589406
4	0.5797058268195057
5	0.2072589701975893
6	0.1010057004189539
7	0.06454701420054341
8	0.04424114706794427
9	0.04339372430089628
>10	0.08295907646905157
>50	0.0013649521056399512
>100	0.003453893095341751
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.686138474796698E-5	8.43069237398349E-6	0.0	0.0
3	2.5292077121950473E-5	0.0	1.686138474796698E-5	8.43069237398349E-6	0.0	0.0
4	3.372276949593396E-5	0.0	2.5292077121950473E-5	8.43069237398349E-6	0.0	0.0
5	3.372276949593396E-5	8.43069237398349E-6	5.9014846617884435E-5	8.43069237398349E-6	0.0	0.0
6	3.372276949593396E-5	1.686138474796698E-5	7.587623136585141E-5	8.43069237398349E-6	0.0	0.0
7	3.372276949593396E-5	1.686138474796698E-5	1.0959900086178538E-4	8.43069237398349E-6	0.0	0.0
8	4.215346186991745E-5	4.215346186991745E-5	1.1802969323576887E-4	1.686138474796698E-5	0.0	0.0
9	4.215346186991745E-5	5.0584154243900945E-5	1.2646038560975236E-4	2.5292077121950473E-5	0.0	0.0
10-14	6.913167746666461E-5	5.3956431193494345E-5	1.652415705300764E-4	3.5408907970730656E-5	0.0	0.0
15-19	8.9365339164225E-5	6.070098509268114E-5	2.428039403707245E-4	5.7328708143087725E-5	0.0	0.0
20-24	1.045405854373953E-4	8.59930622146316E-5	2.8832967919023534E-4	9.77960315382085E-5	0.0	0.0
25-29	1.1634355476097217E-4	1.0116830848780189E-4	3.169940332617792E-4	1.3320493950893915E-4	8.43069237398349E-6	0.0
30-34	3.40599971908933E-4	1.4163563188292262E-4	3.5240294123250993E-4	1.568108781560929E-4	8.43069237398349E-6	1.6861384747966979E-6
35-39	0.0011802969323576886	1.702999859544665E-4	4.0973164937559766E-4	2.1582572477397738E-4	8.43069237398349E-6	1.5175246273170281E-5
40-44	0.002239191894530015	1.9390592460162028E-4	4.8392174226665233E-4	2.664098790178783E-4	1.5175246273170281E-5	3.709504644552735E-5
45-49	0.003035049254634057	2.1076730934958726E-4	5.412504504097401E-4	3.186801717365759E-4	1.686138474796698E-5	4.215346186991745E-5
50-54	0.0036977016752291593	2.4617621732031796E-4	6.002652970276245E-4	3.287970025853561E-4	1.686138474796698E-5	4.215346186991745E-5
55-59	0.0041967986637689815	2.950742330894221E-4	6.879444977170529E-4	3.7432274140486693E-4	2.5292077121950473E-5	4.215346186991745E-5
60-64	0.004604844174669782	3.4734452580811984E-4	7.334702365365636E-4	3.9961481852681746E-4	3.8781184920324055E-5	4.215346186991745E-5
65-69	0.004913407515557578	3.7095046445527355E-4	7.82368252305668E-4	4.232207571739713E-4	4.215346186991745E-5	5.0584154243900945E-5
70-74	0.024046020789075712	3.7095046445527355E-4	8.144048833268051E-4	4.6874649599348205E-4	4.7211877294307545E-5	5.901484661788443E-5
75-79	0.1511488251577256	3.91184126152834E-4	8.413830989235524E-4	5.142722348129929E-4	5.732870814308774E-5	6.913167746666461E-5
80-84	0.1781219823390484	4.198484802243778E-4	8.683613145202996E-4	5.429365888845368E-4	1.0959900086178538E-4	7.587623136585141E-5
85-89	0.17827373480178008	4.29965311073158E-4	9.358068535121675E-4	5.648563890568939E-4	1.8547523222763677E-4	7.587623136585141E-5
90-94	0.17837321697179312	4.518851112455151E-4	0.0010083108079284252	5.985791585528278E-4	1.8547523222763677E-4	7.587623136585141E-5
95-99	0.1785300278499492	4.721187729430754E-4	0.0010757563469202933	6.559078666959156E-4	1.8547523222763677E-4	7.587623136585141E-5
100-104	0.17864468526623536	4.889801576910425E-4	0.0011280266396389912	6.693969744942889E-4	1.8884750917723018E-4	7.587623136585141E-5
105-109	0.17880992683676541	5.092138193886029E-4	0.001190413763206469	6.828860822926628E-4	1.9390592460162028E-4	7.587623136585141E-5
110-114	0.17903755553086298	5.345058965105533E-4	0.0012258226711771996	6.963751900910364E-4	1.9390592460162028E-4	7.587623136585141E-5
115-119	0.179236519870889	5.665425275316905E-4	0.0012764068254211007	7.233534056877834E-4	1.9390592460162028E-4	7.587623136585141E-5
120-124	0.18035780195662882	5.91834604653641E-4	0.001316874148816221	7.587623136585142E-4	1.95592063076417E-4	7.756236984064811E-5
125-129	0.18251268692741898	6.221850971999817E-4	0.0013640860261105287	7.705652829820911E-4	2.0739503239999385E-4	8.9365339164225E-5
130-134	0.18504695305503843	6.441048973723386E-4	0.0013944365186568694	7.82368252305668E-4	2.1076730934958726E-4	1.0116830848780189E-4
135-139	0.1879235052930416	6.710831129690859E-4	0.0014399622574763802	8.04288052478025E-4	2.1582572477397738E-4	1.0959900086178538E-4
140-144	0.19014246352587405	6.913167746666462E-4	0.0014652543345983304	8.464415143479424E-4	2.2425641714796086E-4	1.1802969323576886E-4
145-149	0.19301227120997805	7.132365748390033E-4	0.0015208969042666217	8.531860682471292E-4	2.2762869409755424E-4	1.2140197018536228E-4
150-154	0.19531047795112594	7.216672672129868E-4	0.0015664226430861321	8.59930622146316E-4	2.360593864715377E-4	1.2646038560975236E-4
155-159	0.19702190850304457	7.385286519609538E-4	0.0016035176895316597	8.76792006894283E-4	2.4111780189592786E-4	1.3151880103414246E-4
160-164	0.19867095193139575	7.739375599316844E-4	0.001642298874451984	8.76792006894283E-4	2.4449007884552124E-4	1.3826335493332922E-4
165	0.19952919641506728	7.840543907804646E-4	0.0016524157053007641	8.852226992682665E-4	2.4449007884552124E-4	1.4332177035771934E-4
>>END_MODULE
