##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR479596
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5912096
Total Bases	996.8 Mbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.94947189626149	41.0	40.0	41.0	38.0	41.0
2	40.04483942750591	41.0	40.0	41.0	38.0	41.0
3	40.080248866053594	41.0	40.0	41.0	38.0	41.0
4	40.09572594896971	41.0	40.0	41.0	38.0	41.0
5	40.064526184960464	41.0	40.0	41.0	38.0	41.0
6	40.052358926512696	41.0	40.0	41.0	38.0	41.0
7	40.015309798758345	41.0	40.0	41.0	38.0	41.0
8	39.9932788642133	41.0	40.0	41.0	38.0	41.0
9	39.948433854930634	41.0	40.0	41.0	38.0	41.0
10-14	39.849415063625486	41.0	40.0	41.0	38.0	41.0
15-19	39.772749055495716	40.8	40.0	41.0	37.2	41.0
20-24	39.54524530724806	40.6	39.2	41.0	36.2	41.0
25-29	39.087760685888725	40.0	38.0	41.0	35.0	41.0
30-34	38.46510740015047	39.8	36.6	41.0	35.0	41.0
35-39	37.81692787126596	38.8	35.0	41.0	34.0	41.0
40-44	37.153114766742625	36.8	35.0	39.8	34.0	41.0
45-49	36.38720930783262	35.4	35.0	38.8	33.8	40.8
50-54	35.77136812392762	35.0	35.0	37.0	33.0	39.4
55-59	35.32074205154991	35.0	35.0	36.0	33.0	38.8
60-64	34.61500418802401	34.8	33.8	35.0	32.2	37.0
65-69	35.01698730873112	35.0	35.0	35.0	33.0	36.8
70-74	34.97518612688292	35.0	35.0	35.0	33.0	36.0
75-79	34.968036040010176	35.0	35.0	35.0	33.0	36.6
80-84	34.95635760988996	35.0	34.2	35.0	33.0	37.8
85-89	35.73128741481871	36.0	34.8	36.2	33.2	39.0
90-94	39.90068935345682	41.0	40.0	41.0	37.6	41.0
95-99	39.74003288033394	41.0	40.0	41.0	36.6	41.0
100-104	39.51893546246551	41.0	39.2	41.0	35.4	41.0
105-109	39.372971687471455	40.8	39.0	41.0	35.0	41.0
110-114	39.097585902951664	40.4	38.0	41.0	35.0	41.0
115-119	38.605767921175065	40.0	36.6	41.0	35.0	41.0
120-124	37.48276368749826	38.4	34.8	40.2	33.8	40.6
125-129	37.273279758799625	37.4	35.0	40.0	34.0	41.0
130-134	36.64054629691877	35.8	35.0	39.2	34.0	41.0
135-139	35.93879694729448	35.0	35.0	37.6	33.0	40.0
140-144	35.49231722740563	35.0	35.0	36.4	33.6	38.8
145-149	35.13051923135221	35.0	35.0	35.4	34.0	37.0
150-154	34.841111967266635	35.0	35.0	35.0	33.4	36.0
155-159	34.6696838547561	35.0	35.0	35.0	33.0	35.8
160-164	34.567113805258586	35.0	35.0	35.0	33.0	35.0
165-169	34.50521231671424	35.0	35.0	35.0	33.0	35.0
170-174	34.43488170890899	35.0	34.6	35.0	33.0	35.0
175-176	33.729469477789365	34.5	33.0	35.0	31.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	11.0
30	184.0
31	1969.0
32	12383.0
33	52522.0
34	184781.0
35	639299.0
36	1705443.0
37	2200696.0
38	1073637.0
39	40957.0
40	214.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	25.10412550811083	24.682464560791974	25.22724597164863	24.98616395944856
2	24.69288387739306	24.7259685904965	25.55181783245739	25.02932969965305
3	24.715819905495444	24.865039403960964	25.37797085838931	25.04116983215428
4	24.722264320471115	25.00348438185036	25.300874681331294	24.97337661634723
5	24.80336922810455	25.0211938371772	25.244870854600464	24.930566080117778
6	24.64785754493838	24.944385206194216	25.343972763635776	25.063784485231633
7	24.719202800495797	24.938904916293648	25.34760937576115	24.994282907449406
8	24.894470590464024	24.818710656931145	25.296172457280804	24.990646295324026
9	24.741885111473156	24.66478893441514	25.185044356519242	25.408281597592463
10-14	24.89749489859434	24.732182968612147	25.15548462000617	25.21483751278734
15-19	24.921019550426788	24.67799575649651	25.088760399019232	25.312224294057472
20-24	24.97028465031691	24.63281042797681	25.028409552212956	25.36849536949332
25-29	24.94587706288937	24.759043154914938	25.00435378586545	25.290725996330238
30-34	24.889565392713514	24.675884830016294	25.04624079175981	25.38830898551038
35-39	24.998034538004795	24.692471164203017	24.9567395387355	25.352754759056683
40-44	25.07999193517832	24.591031674722466	24.91901687658658	25.409959513512636
45-49	25.219661521057844	24.62241140874573	24.772266891471315	25.38566017872511
50-54	25.207171872716543	24.561857588239434	24.801589825334368	25.429380713709655
55-59	25.190944125399856	24.6596232537496	24.758075646944842	25.391356973905697
60-64	25.11622274063209	24.629779354056495	24.823504219146646	25.430493686164773
65-69	25.13895579503445	24.672356470530925	24.770413065011123	25.418274669423504
70-74	25.090698797854433	24.710542589294896	24.73210854492214	25.46665006792853
75-79	25.085610923773906	24.737514411132704	24.676473453746354	25.50040121134704
80-84	25.10023179596542	24.81856180955113	24.616761974095144	25.464444420388304
85-89	25.129876104853505	24.810151932580254	24.596004530372984	25.46396743219325
90-94	24.904034118044525	25.17909384982965	24.967921709448156	24.948950322677675
95-99	24.937732123117723	25.05712412293237	24.96517676882215	25.03996698512776
100-104	24.969617637114418	25.02832179369323	24.909671028600837	25.092389540591515
105-109	25.074613316902266	24.911661210527434	24.816083434529325	25.197642038040975
110-114	25.148900686739722	24.955465296490775	24.669047501063687	25.22658651570582
115-119	25.06305333070633	24.96788690975756	24.72642227897736	25.24263748055875
120-124	25.044807397507174	24.972683799303137	24.734193813772002	25.248314989417686
125-129	25.06478336207893	25.04269875556294	24.669532024136036	25.222985858222096
130-134	25.161174379601814	24.924170293512436	24.617308251932553	25.297347074953198
135-139	25.244920969470613	25.01344968944781	24.571133034542186	25.17049630653939
140-144	25.154621404146287	25.04451539317369	24.644397253390796	25.15646594928923
145-149	25.14133183349674	25.16145659402088	24.668080914757674	25.029130657724707
150-154	25.054162298516943	25.174860939271284	24.782843511511288	24.98813325070049
155-159	25.017402690917734	25.35768001805608	24.75748514690178	24.867432144124415
160-164	24.926727370441046	25.38975551644993	24.812355809208523	24.8711613039005
165-169	24.91545539101374	25.577362431713635	24.80579383225326	24.701388345019364
170-174	24.78879558417529	25.74368025252062	24.873650691067986	24.593873472236105
175-176	24.804921923346708	25.7816295628393	24.762067187456278	24.65138132635771
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	0.0
19	2.0
20	8.5
21	17.5
22	40.0
23	98.5
24	231.0
25	512.0
26	1089.5
27	2079.5
28	3786.5
29	6489.0
30	10651.5
31	16902.5
32	25395.5
33	36350.0
34	50078.0
35	67034.0
36	86196.5
37	107629.5
38	130625.0
39	152908.0
40	175073.5
41	195879.0
42	212930.0
43	225929.5
44	234705.0
45	239296.0
46	238959.5
47	234635.5
48	226664.5
49	216108.0
50	205251.5
51	193803.5
52	183016.0
53	174169.5
54	166435.5
55	161270.0
56	158929.0
57	157531.0
58	156905.0
59	156144.0
60	154790.0
61	152200.0
62	147113.0
63	139250.0
64	128486.0
65	115767.0
66	101808.5
67	86603.5
68	71499.5
69	57029.5
70	43842.5
71	32923.0
72	23986.0
73	16595.5
74	11134.5
75	7265.5
76	4489.5
77	2692.5
78	1567.0
79	887.0
80	476.5
81	243.0
82	137.5
83	76.0
84	28.0
85	12.0
86	3.0
87	1.0
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	812.0
92-93	1910.0
94-95	2964.0
96-97	4054.0
98-99	4925.0
100-101	5678.0
102-103	6370.0
104-105	7565.0
106-107	8044.0
108-109	8499.0
110-111	8784.0
112-113	9488.0
114-115	9987.0
116-117	10547.0
118-119	10862.0
120-121	12011.0
122-123	12418.0
124-125	13178.0
126-127	13915.0
128-129	14962.0
130-131	15488.0
132-133	44157.0
134-135	71868.0
136-137	71967.0
138-139	76079.0
140-141	66829.0
142-143	63118.0
144-145	62688.0
146-147	78294.0
148-149	60291.0
150-151	58491.0
152-153	58179.0
154-155	60650.0
156-157	63567.0
158-159	65338.0
160-161	67608.0
162-163	69463.0
164-165	71653.0
166-167	75127.0
168-169	78789.0
170-171	81957.0
172-173	88331.0
174-175	279952.0
176-177	4015239.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.75280888987089
#Duplication Level	Percentage of total
1	90.05235531823487
2	8.604926131513075
3	0.9459586796049262
4	0.20186554896563044
5	0.08783706951750493
6	0.04376838998331562
7	0.02732287759660014
8	0.012716441071589703
9	0.009617175428116338
>10	0.010179023529326953
>50	0.0
>100	0.003453344555014057
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.6914475001759105E-5	1.6914475001759105E-5	0.0	0.0
2	0.0	1.6914475001759105E-5	1.6914475001759105E-5	3.382895000351821E-5	0.0	0.0
3	0.0	1.6914475001759105E-5	1.6914475001759105E-5	3.382895000351821E-5	0.0	0.0
4	0.0	1.6914475001759105E-5	3.382895000351821E-5	3.382895000351821E-5	0.0	0.0
5	0.0	1.6914475001759105E-5	5.0743425005277315E-5	6.765790000703642E-5	0.0	0.0
6	0.0	1.6914475001759105E-5	6.765790000703642E-5	8.457237500879553E-5	0.0	0.0
7	0.0	3.382895000351821E-5	1.0148685001055463E-4	1.0148685001055463E-4	0.0	0.0
8	0.0	3.382895000351821E-5	1.0148685001055463E-4	1.1840132501231374E-4	0.0	0.0
9	0.0	3.382895000351821E-5	1.0148685001055463E-4	1.1840132501231374E-4	0.0	0.0
10-14	1.0148685001055463E-5	5.0743425005277315E-5	1.1840132501231373E-4	1.31932905013721E-4	0.0	0.0
15-19	3.382895000351821E-5	9.472106000985099E-5	1.454644850151283E-4	2.0635659502146107E-4	0.0	0.0
20-24	3.382895000351821E-5	1.1163553501161009E-4	1.8605922501935016E-4	2.5710002002673837E-4	0.0	0.0
25-29	4.397763500457367E-5	1.1840132501231373E-4	2.2327107002322017E-4	2.976947600309602E-4	0.0	0.0
30-34	1.7252764501794288E-4	1.7591054001829472E-4	2.63865810027442E-4	3.146092350327194E-4	0.0	0.0
35-39	8.592553300893625E-4	2.1650528002251654E-4	3.21375025033423E-4	3.21375025033423E-4	0.0	1.3531580001407284E-5
40-44	0.00211769227022024	2.3680265002462745E-4	3.9241582004081126E-4	3.5182108003658944E-4	0.0	2.0297370002110926E-5
45-49	0.0029262041753043253	2.3680265002462745E-4	4.6007372004784765E-4	4.1271319004292214E-4	0.0	5.4126320005629136E-5
50-54	0.0034031923703539317	2.3680265002462745E-4	5.784750450601613E-4	4.668395100485513E-4	0.0	6.765790000703642E-5
55-59	0.0037888424003940393	2.773973900288493E-4	6.359842600661425E-4	5.27731620054884E-4	6.765790000703642E-6	7.104079500738825E-5
60-64	0.004076388475423944	3.2475792003377487E-4	6.901105800717715E-4	5.446460950566431E-4	1.6914475001759105E-5	1.0148685001055463E-4
65-69	0.004404529290458071	3.4505529003588575E-4	7.543855850784562E-4	5.683263600591059E-4	2.0297370002110926E-5	1.1501843001196191E-4
70-74	0.02303413205739555	3.5858687003729306E-4	8.32192170086548E-4	5.953895200619204E-4	3.382895000351821E-5	1.31932905013721E-4
75-79	0.14747730754033764	3.822671350397558E-4	9.133816500949916E-4	6.089211000633277E-4	3.382895000351821E-5	1.3531580001407284E-4
80-84	0.17440515174313814	4.0933029504257036E-4	9.404448100978062E-4	6.123039950636796E-4	5.750921500598096E-5	1.3531580001407284E-4
85-89	0.17460812544315923	4.4654214004644034E-4	0.0010081027101048426	6.42750050066846E-4	9.810395501020281E-5	1.3531580001407284E-4
90-94	0.17469946360816874	4.566908250474958E-4	0.0010656119251108236	6.799618950707161E-4	1.0148685001055463E-4	1.3531580001407284E-4
95-99	0.17485169388318458	4.566908250474958E-4	0.0011028237701146935	7.442369000774006E-4	1.0148685001055463E-4	1.3531580001407284E-4
100-104	0.17502083863320217	4.837539850503104E-4	0.0011434185101189154	7.814487450812707E-4	1.0148685001055463E-4	1.3531580001407284E-4
105-109	0.17519674917322045	5.040513550524213E-4	0.0011975448301245444	7.949803250826779E-4	1.0148685001055463E-4	1.3531580001407284E-4
110-114	0.17548767814325072	5.243487250545323E-4	0.0012178422001266555	8.152776950847889E-4	1.0148685001055463E-4	1.3531580001407284E-4
115-119	0.17583611632828694	5.378803050559395E-4	0.0012584369401308776	8.389579600872517E-4	1.0148685001055463E-4	1.3531580001407284E-4
120-124	0.1771791256434266	5.480289900569949E-4	0.0013024145751354512	8.69404015090418E-4	1.0148685001055463E-4	1.6576185501723925E-4
125-129	0.17953362056367148	5.750921500598096E-4	0.0013599237901414322	8.897013850925291E-4	1.1163553501161009E-4	1.6914475001759106E-4
130-134	0.182067408918935	5.953895200619204E-4	0.0014275816901484686	8.964671750932327E-4	1.1840132501231373E-4	1.6914475001759106E-4
135-139	0.18517628942425832	6.292184700654388E-4	0.001495239590155505	9.133816500949916E-4	1.2178422001266555E-4	1.6914475001759106E-4
140-144	0.18776082120452714	6.42750050066846E-4	0.0015392172251600786	9.201474400956953E-4	1.3531580001407284E-4	1.6914475001759106E-4
145-149	0.19086970170985046	6.562816300682533E-4	0.0015730461751635966	9.370619150974545E-4	1.3531580001407284E-4	1.8267633001899835E-4
150-154	0.19328170584510132	6.731961050700124E-4	0.0016508527601716886	9.607421800999171E-4	1.3531580001407284E-4	1.8605922501935016E-4
155-159	0.19507802309028813	6.765790000703642E-4	0.0016779159201745033	9.742737601013244E-4	1.3531580001407284E-4	1.8605922501935016E-4
160-164	0.19683374559547068	6.901105800717715E-4	0.0017117448701780215	9.810395501020281E-4	1.3531580001407284E-4	1.8605922501935016E-4
165	0.1977809561955692	6.934934750721233E-4	0.0017421909251811878	9.810395501020281E-4	1.3531580001407284E-4	1.8605922501935016E-4
>>END_MODULE
